Synthetic α-tocopherol has eight isomeric configurations including four 2R (RSS, RRS, RSR, RRR) and four 2S (SRR, SSR, SRS, SSS). Only the RRR stereoisomer is naturally synthesised by plants. A ratio of 1·36:1 in biopotency of RRR-α-tocopheryl acetate to all-rac-α-tocopheryl acetate is generally accepted; however, studies indicate that neither biopotency of α-tocopherol stereoisomers nor bioavailability between them is constant, but depend on dose, time, animal species and organs. A total of forty growing young male mink were, after weaning, assigned one of the following treatments for 90 d: no α-tocopherol in diet (ALFA_0), 40 mg/kg RRR-α-tocopheryl acetate (NAT_40), 40 mg/kg all-rac-α-tocopheryl acetate (SYN_40) and 80 mg/kg feed all-rac-α-tocopheryl acetate (SYN_80). Mink were euthanised in CO2 and blood was collected by heart puncture. Mink were pelted and liver, heart, lungs, brain and abdominal fat were collected for α-tocopherol stereoisomer analysis. The proportion of RRR-α-tocopherol decreased in all organs and plasma with increasing amount of synthetic α-tocopherol stereoisomers in the diet (P≤0·05), whereas the proportion of all synthetic α-tocopherol stereoisomers increased with increasing amount of synthetic α-tocopherol stereoisomers in the diet (P≤0·05). The proportion of α-tocopherol stereoisomers in plasma, brain, heart, lungs and abdominal fat showed the following order: RRR>RRS, RSR, RSS>Σ2S, regardless of α-tocopherol supplement. The liver had the highest proportion of Σ2S stereoisomers, and lowest proportion of RRR-α-tocopherol. In conclusion, distribution of α-tocopherol stereoisomers differs with dose and form of α-tocopherol supplementation. The results did also reveal the liver’s role as the major organ for accumulation of Σ2S α-tocopherol stereoisomers.

The biological function of the stereoisomers of α-tocopherol is believed to depend on their bioavailability. Assessment of bioavailability within the body is therefore considered to be a good and easy way to predict biological value. The separation of α-tocopherol methyl ethers by chiral column HPLC is a good and easy tool with which to study the distribution of α-tocopherol stereoisomers. The objective of this investigation was to evaluate the bioavailability and distribution of the stereoisomers of α-tocopherol in the plasma and tissue in growing rats fed 25, 50, 100 or 200mg/kg diet of either RRR- or all-rac-α-tocopheryl acetate for 10d. The ratio between the two vitamin E sources based on their α-tocopherol concentration in plasma and tissues varied in the plasma between 1·04 and 1·74 and in tissues, ratios of 0·84–1·24 for liver, 0·34–1·59 for lung and 0·75–1·50 for spleen were obtained. An increasing dietary level of all-rac-α-tocopheryl acetate decreased the proportion of RRR-α-tocopherol, whereas the other stereoisomers were not affected. RRS-α-Tocopherol was present in the highest proportion, followed by RSR-, RSS- and RRR-α-tocopherol. In contrast to the other tissues and plasma, the liver contained the highest proportion (29–33%) of the four 2S stereoisomers of total α-tocopherol. Rats fed RRR-α-tocopheryl acetate for 10d showed a significant increase in the plasma and tissue content of RRR-α-tocopherol and a simultaneous decrease in the other three 2R isomers, whereas the absolute content of the 2S isomers was unaffected. In adipose tissue, concentrations of the three synthetic 2R isomers remained constant, whereas there was a steep increase in the content of RRR-α-tocopherol.

The effect of supplementation with different levels of all-rac-α-tocopheryl acetate and the inclusion of different dietary contents of PUFA on the deposition of α-tocopherol stereoisomers in liver and thigh of chickens was evaluated. Ninety-six 1-d-old Ross female broiler chickens were randomly distributed into eight experimental treatments (three replicates each) resulting from four levels of α-tocopheryl acetate without supplementation and supplemented with 100, 200 and 400 mg α-tocopheryl acetate/kg and two levels of dietary PUFA (15 and 61 g/kg). The feeds supplemented with α-tocopheryl acetate contained a similar proportion of each stereoisomer. The diets without α-tocopheryl acetate had the following α-tocopherol stereoisomers (%): RRR 35·1, RRS 24·5, RSR 25·3, RSS 13·9 and total 2S forms 1·3. Consumption of different levels of α-tocopheryl acetate did not lead to statistical differences in α-tocopherol stereoisomer proportion in the liver and thigh. In general, the stereoisomer profiles in the tissues studied were similar, responding to the stereoisomer profile of the diet. Both tissues preferentially accumulated 2R stereoisomer (69–100%). However, when α-tocopheryl acetate was used the discrimination was not specific for the RRR α-tocopherol form. Furthermore, the 2R:2S ratio had a tendency to increase as the polyunsaturation level of the diet increased.