Bell pepper or sweet pepper (Capsicum annuum L. var. grossum) is highly susceptible to high-temperature stress (HT). Hence, search for donor across C. annuum for HT tolerance was undertaken by following Temperature Induction Response (TIR) technique. The induction and challenging temperature requirement for TIR screening were standardized in 1 d-old Capsicum seedlings. Forty Capsicum genotypes were evaluated based on the recovery growth (RG) and per cent reduction in recovery growth (%RRG) of the seedlings. The genotypes Punjab Guchhedar and Ajeet 1 were found to have maximum cellular level tolerance (CLT) to HT with higher RG and lower %RRG compared to the non-induced seedlings and HDC 75 was found to have minimum CLT. In order to confirm the findings, another experiment was conducted under managed stress and control conditions. Absolute yield obtained from both the environments were used to calculate stress tolerance indices such as heat susceptibility index, tolerance index, stress tolerance index, mean productivity, geometric mean productivity and yield stability index. Based on these tolerance indices, Punjab Guchhedar and Ajeet 1 were found to be highly tolerant and HDC 75 as highly susceptible. Further, the combined result of TIR and tolerance indices also gave the same result confirming Punjab Guchhedar and Ajeet 1 can be used as a donor for the future breeding programme aimed at evolving high-temperature-tolerant bell pepper cultivars. The result also confirms the fitness of TIR technique to screen Capsicum genotypes for tolerance to HT based on variability in acquired thermotolerance.

Yellow and purple nutsedge and southern root-knot nematode are common pests in intensively managed chile pepper production. Greenhouse studies were conducted to identify relationships among nutsedge species, chile peppers, and root-knot nematode. All practical combinations of the plant species and nematodes were grown together in pots for 14 wk. Both nutsedges and root-knot nematode reduced chile pepper height over time with no interaction. Competition from purple nutsedge or either nutsedge species plus nematodes reduced chile pepper top weight more than yellow nutsedge or nematodes alone. All pest combinations reduced chile pepper root weight. Chile pepper and nematodes individually reduced rates of shoot production, as well as top and belowground weights of yellow and purple nutsedge. Chile pepper and purple and yellow nutsedge hosted root-knot nematode host race 3. Nematode egg production was greater on chile pepper roots than on either nutsedge species. Purple nutsedge growing with chile pepper increased nematode production g−1 of chile pepper root. Purple nutsedge grown with chile pepper was the most detrimental for chile pepper growth, but most efficient for maintaining the root-knot nematode population on the least amount of root biomass. Since root-knot nematode infests yellow and purple nutsedge, and few nematicides are available for nematode control, root-knot nematode cannot be managed with crop rotation if nutsedge is not controlled.

Field research was conducted in southern New Mexico to determine the effect of halosulfuron rate, timing of application, and adjuvant on purple nutsedge suppression and chile pepper injury under cultivated conditions. POST-directed treatments of 36 or 53 g ai/ha were applied 2 or 4 wk after thinning direct-seeded chile pepper. Herbicide treatments included a nonionic surfactant or methylated seed oil plus ammonium sulfate plus polyacrylamide polymer. Herbicide rate, adjuvant, and timing of the application had little effect on overall nutsedge suppression; however, herbicide treatment proved to be an important component for managing purple nutsedge in this experiment. Yields were higher with halosulfuron treatment compared with the weedy control. Whereas halosulfuron treatment with 53 g/ha increased chile yield relative to the weedy control, injury and yield results comparing 36 and 53 g/ha rates consistently suggested that halosulfuron should be applied at rates ≤ 36 g/ha in a single application to avoid possible injury. Halosulfuron treatment combined with cultivation and effective crop rotation may reduce the impact of purple nutsedge interference in chile pepper.

This is the first report of Bactra bactrana (Kennel, 1901) (Lepidoptera: Tortricidae) attacking a major solanaceous crop, sweet pepper Capsicum annuum L. The infestation was detected in two greenhouses at the area of Tympaki (Southern Crete, Greece). The moth larvae caused typical symptoms of a fruit borer with numerous small holes on the surface of the peppers and extensive damage on the inside of the fruit as a result of the feeding activity. Unknown factors facilitated this major shift in host range since B. bactrana is typically a stem borer of sedges. In addition, the pest status of B. bactrana is currently under question, as in both cases the infestations by the moth were associated with significant yield losses. B. bactrana was moderately controlled with chemicals registered for Lepidoptera management in sweet pepper due to the boring nature of the infestation. Some comparative taxonomic notes are provided to facilitate accurate pest discrimination of related Bactra species. Finally, biological attributes of the species are summarized and are discussed from pest control and ecological perspectives. Because Bactra species have been used in augmentative releases for the control of sage, the implications of our findings on the release of biocontrol agents are placed in perspective.

A primary challenge of managing vegetable production on a small land base is the maintenance and building of soil quality. Previous studies have demonstrated the benefits of cover crops for improved soil quality; however, small growers struggle to fit cover crops into rotations. Small-scale growers with limited available land are under significant pressure to maximize their saleable yield and often work to maximize output by using intensive cropping practices that may include both early and late season crops, thus limiting the typical shoulder season windows in which cover crops can be grown. In-season living mulches may be an effective strategy to provide small-scale growers the benefits of cover crops with less land commitment than cover crops used in typical rotations. However, research on living mulches is generally not suited to small-scale organic production systems due to the typical reliance on chemical herbicide to suppress mulches. An experiment was designed with the goal of evaluating living mulch systems for space-limited organic vegetable production. In a 2-year study, four living mulch crops (buckwheat (Fagopyrum esculentum), field pea (Pisum sativum), crimson clover (Trifolium incarnatum) and medium red clover (Trifolium pratense)) and a cultivated control with no mulch cover were planted in early spring each year. Snap beans (Phaseolus vulgaris var. Tavera), transplanted bell peppers (Capsicum annuum var. Revolution), and transplanted fall broccoli (Brassica oleracea var. Imperial) were then planted directly into living mulches. During each summer growing season, living mulches and weeds were mown between-rows and hand-weeded in-row approximately every 10–14 days as needed for management. Labor times for mowing and cultivation were found to be higher in all treatments relative to the cultivated control. An inverse relationship between living mulch biomass and weed biomass was observed, demonstrating that living mulches may contribute to weed suppression. However, lower vegetable yields were seen in the living mulch treatments, most likely due to resource competition among vegetables, living mulches and weeds. High pre-existing weed seedbank and drought conditions likely increased competition and contributed to reduced vegetable yield.

Root-knot nematodes (RKNs), Meloidogyne spp., are considered, worldwide, as one of the main pathogens of solanaceous crops, including pepper (Capsicum spp.). Restrictions on the use of standard nematicides have motivated the development and use of resistant cultivars and rootstocks. Three genes in pepper, called Me1, Me3 and N, confer resistance to the three main RKN species (Meloidogyne incognita, Meloidogyne javanica and Meloidogyne arenaria). However, their effectiveness seems to be limited because nematode populations that have overcome the resistance have been found, leading to a search for new sources of resistance and strategies to preserve their effectiveness. In two greenhouses and over a 7-month growing period, we evaluated the resistance to M. incognita and the agronomic behaviour as rootstocks of nine pepper (Capsicum annuum) accessions – HDA330 (Me1 carrier), Serrano Criollo de Morelos, (Me3 carrier), Yolo Wonder (partially resistant) and another six accessions of unknown resistance originating from cultivars well adapted to the local growing conditions. The resistance conferred by the Me1 gene was more robust than that conferred by Me3. Resistance to M. incognita was found in four new accessions: P13, CTL, CT5, and P14. In P13, the level of resistance was similar to that of HDA330. The resistant accessions showed better agronomic behaviour than the susceptible accessions, which was most noticeable towards the final of the growing period. Some accessions constitute a potential resource for use in the genetic breeding of RKN-resistant rootstocks.

Next-generation sequencing technique has been known as a useful tool for de novo transcriptome assembly, functional annotation of genes and identification of molecular markers. This study was carried out to mine molecular markers from de novo assembled transcriptomes of four chilli pepper varieties, the highly pungent ‘Saengryeg 211’ and non-pungent ‘Saengryeg 213’ and variably pigmented ‘Mandarin’ and ‘Blackcluster’. Pyrosequencing of the complementary DNA library resulted in 361,671, 274,269, 279,221, and 316,357 raw reads, which were assembled in 23,607, 19,894, 18,340 and 20,357 contigs, for the four varieties, respectively. Detailed sequence variant analysis identified numerous potential single-nucleotide polymorphisms (SNPs) and simple sequence repeats (SSRs) for all the varieties for which the primers were designed. The transcriptome information and SNP/SSR markers generated in this study provide valuable resources for high-density molecular genetic mapping in chilli pepper and Quantitative trait loci analysis related to fruit qualities. These markers for pepper will be highly valuable for marker-assisted breeding and other genetic studies.

Using flow cytometry, we determined relative DNA amounts expressed as C-values, of large numbers of nuclei isolated from embryo root tips in three seed lots of pepper (Capsicum annuum) and tomato (Lycopersicon esculentum). The nuclei in embryos of fully matured dry pepper and tomato seeds predominantly revealed 2C signals, which indicates that the cells of maturing seeds have arrested at G1 phase of the cell cycle. Priming of pepper and tomato seeds in −1.1, −1.3 or −1.5 MPa polyethylene glycol (PEG) for 14 days reduced the mean time to germination. In all seed lots an induction of 4C signals was found after priming, indicating that during priming the cells of the embryonic root tip had replicated their DNA and arrested at the G2 phase of the cell cycle. For both tomato and pepper, the frequency of 4C signals was highest at the lowest PEG concentration (osmotic potential of −1.1 MPa). A significant inverse correlation was found between the frequency of root tip cells expressing 4C DNA signals and the mean time to germination for each individual pepper and tomato seed lot. However, the effect of a specific osmotic treatment differed between seed lots of the same cultivar, with regard to both the decrease in the mean time to germination and to the percentage of 4C cells, suggesting specific differences among seed lots in the response to priming. The present results indicate that flow cytometry can be used to follow the progress in nuclear replication activities during priming. The advancement in DNA synthesis can thus be used to measure the efficiency of a priming treatment with regard to enhancement of seed lot germination rate.

The effect of two priming treatments in PEG solutions on nuclear replication stages of pepper (Capsicum annuum L.) embryos were evaluated using flow cytometry. Priming at −1.1 MPa for 10 d induced almost 40% of nuclei in the embryo root tips to enter the synthetic phase of nuclear division, while no DNA synthesis occurred during 6 d at−1.5 MPa and in less than 10% of the nuclei at 10 d. Both priming treatments effectively increased seed performance. A decrease of 2.7 d in mean germination time (MGT) was observed after priming in PEG solution at −1.1 MPa, while the treatment at−1.5 MPa lowered the MGT by 2.1 d. Primed and untreated seeds were subjected to controlled deterioration treatments by exposure to 45°C after equilibration at 75% RH. Both osmotic treatments considerably lowered seed tolerance to adverse storage conditions as compared with untreated seeds. However, seeds in which DNA replication was induced by priming were more sensitive to controlled deterioration than seeds in which priming did not induce nuclei to enter the synthetic phase. This could be a consequence of a higher DNA content, which is a more vulnerable target for mutation inducing factors. Alternatively the increased sensitivity could be a consequence of their more advanced progress in germinative events, making seeds less resistant to deteriorative factors imposed during storage. On the whole, from the present results it appears that the nucleic acid DNA content of the embryo only plays an additive role in influencing seed storability.

The bell-type pepper cultivar Golden California Wonder, a source of resistance to southern blight, was crossed with the susceptible cultivar Sweet Banana. The F1, F2 and backcross progenies of this cross and the parental lines were evaluated in an inoculated field study for their reactions to Sclerotium rolfsii Sacc. A categorical rating system was used to rate plants for severity of wilting and severity of stem-lesion development. The plants within all three non-segregating populations (the resistant parent, the susceptible parent and the F1) displayed a range of reactions to S. rolfsii. Variable reactions to S. rolfsii among homogeneous pepper populations were expected because this phenomenon is well documented in the literature and we had observed similar responses in field trials conducted over multiple years. The high level of variation for reactions to S. rolfsii exhibited by the parental and F1 hybrid populations and the categorical system of rating individual plants placed severe limitations on the effective use of any quantitative type of analysis. Accordingly, a weighting procedure was used to correct the F2 and backcross populations based on frequencies of apparent mis-classifications in the parental and F1 populations. Examination of the comparative frequency distributions of the parental and progeny populations and examination of the weighted segregation data suggest that the southern blight resistance in ‘Golden California Wonder’ is conditioned by a single recessive gene. The level of resistance conditioned by this reputed recessive gene appears high enough to recommend it for use in pepper breeding programmes.

The developmental regulation and organ-specific expression of shikimate dehydrogenase was studied in seedlings of Capsicum annuum L. during their early development. The results obtained suggest that there is both a developmental regulation and an organ-specific expression of shikimate dehydrogenase in seedlings of C. annuum, seen mainly in the cotyledons. For example, shikimate dehydrogenase isoenzyme 3 was differentially expressed in cotyledons, but not at all in roots or hypocotyls. When the amounts of shikimate dehydrogenase were compared with the end-products of the shikimate pathway, chlorogenic acid and lignins, it was observed that the fall in shikimate dehydrogenase activity in cotyledons coincided with a decrease in the concentrations of phenolics during primary leaf development. However, this did not seem to be the case with the hypocotyls, where the fall in phenolics was not related to the amount of shikimate dehydrogenase but rather to an increase in lignin deposition, supporting the view that chlorogenic acid might act as a precursor of the aromatic moiety of cinnamoyl alcohols during cell wall lignification.