Chloris spp. are warm-season grasses that outcompete crops for scarce resources throughout Australia. In Queensland, mild winters and increased adoption of conservation tillage practices have led to an increase of this warm-season grass family in winter crops. The objective of this study is to understand whether droplet size (nozzle type) effects herbicide efficacy of summer perennial grasses, as previous research found no effect of droplet size (nozzle type) on herbicide efficacy of winter annual grasses. A study to compare droplet-size (nozzle type) effects on control of windmillgrass and its domesticated relative, rhodesgrass, was conducted at the University of Queensland in Gatton, QLD, Australia. Results showed little difference in dry weight reductions for windmillgrass or rhodesgrass across droplet size (nozzle type). Paraquat applications with the TTI nozzle resulted in significantly lower dry weight reductions compared with other droplet-size sprays (nozzle types) for rhodesgrass. Glyphosate, imazamox plus imazapyr, and clodinafop resulted in commercially acceptable control for both species, regardless of the droplet size (nozzle type) selected, indicating droplet size (nozzle type) has relatively little impact on the efficacy of these herbicides. Proper nozzle selection can result in control of Chloris spp., a hard to control weed species, while reducing the occurrence of spray drift to nearby sensitive areas.

Understanding the mechanism of herbicide resistance is fundamental for designing sustainable weed control strategies and exploiting herbicides rationally. Shortawn foxtail is a problem grass weed infesting several important crops in China. The repeated use of acetyl-CoA carboxylase (ACCase)-inhibiting herbicides has resulted in herbicide resistance in this weed. The ACCase gene of resistant individuals of a shortawn foxtail population (JSLS-1) has an Ile-2041-Thr mutation. F2 generation seeds, originated from the same heterozygous plant, were harvested, and two homozygous mutant (JSLS-1RR) and wild (JSLS-1SS) populations for the Ile-2041-Thr mutation were obtained. In whole plants, the JSLS-1RR population conferred high resistance to fenoxaprop and clodinafop, moderate resistance to haloxyfop, low resistance to pinoxaden, and no obvious resistance to clethodim and sethoxydim, compared with JSLS-1SS and a proven susceptible population (HNXY-1). A derived cleaved amplified polymorphic sequence (dCAPS) marker was developed to rapidly detect the rare Ile-2041-Thr mutation in the shortawn foxtail population. This is the first report of the cross-resistance pattern of Ile-2041-Thr mutation, and the robust dCAPS marker could quickly detect this mutation in shortawn foxtail.

Shortawn foxtail is a competitive annual grass weed widely spread in east, south-central, and southwest China and parts of the Yellow River basin. One shortawn foxtail population (JSQT-1) resistant to fenoxaprop was identified in Jiangyan, Jiangsu province. Whole-plant experiments determined that the resistant population conferred high-level resistance to fenoxaprop (93-fold), clodinafop (21-fold), sethoxydim (107-fold), mesosulfuron (41-fold), and pyroxsulam (12-fold); moderate-level resistance to haloxyfop (8-fold), clethodim (9-fold), and pinoxaden (8-fold), and no resistance to isoproturon. Molecular analyses confirmed that the Ile-1781-Leu mutation was present in the resistant population. A dCAPS marker was used to detect the Ile-1781-Leu mutation. All 97 plants of the resistant population analyzed were homozygous mutants at the 1781 position. Our study established the first case of fenoxaprop resistance in shortawn foxtail, determined cross resistance to other herbicides, and elucidated that the molecular basis of resistance resulted from, at least partly, an Ile to Leu mutation at amino acid position 1781 in the plastid ACCase.

Littleseed canarygrass is a major weed of winter-season crops, although it is most dominant in wheat-growing regions in the Indo-Gangetic Plains of India, Pakistan, Nepal, and Bangladesh. Resistance in this species to photosystem II–inhibiting herbicide isoproturon was first recorded in 1992, and has since spread to several Indian states covering more than a million ha. Genetic studies and resistance characterization from multiple locations indicate independent evolution of resistance due to continuous use of isoproturon and monoculture rice–wheat-cropping system. Isoproturon-resistant biotypes were found cross-resistant to diclofop, but not to chlortoluron, which has the same mode of action as isoproturon. The isoproturon-resistance mechanism is metabolic degradation, mediated by P-450 monooxygenase enzymes. This type of resistance could become serious and lead to the evolution of multiple resistances to herbicides of different modes of action. Adoption of fenoxaprop-P, clodinafop, and sulfosulfuron in isoproturon-resistant areas since 1997 initially led to high yields, but resulted in a weed flora shift which eventually reduced yields and increased the cost of weed management. Although isoproturon recommendation has been withdrawn from rice–wheat cropping zones, resistance in littleseed canarygrass is spreading in other areas where isoproturon has been used for several years because it is inexpensive and has broad-spectum weed control. Management factors, such zero or minimum tillage, early planting after rice harvest, and alternative herbicides provide effective control of resistant biotypes. However, lower efficacy of these herbicides has been observed in the field, although multiple resistances have yet to be confirmed. Herbicide rotations, mixtures, and sequences are beneficial, but only in the short term. Improved cultivation practices are also helpful; however, no current single system is sustainable. An integration of tillage method, planting time, varietal selection, crop rotation, timing and method of herbicide application, optimum dose, and sanitation practices is crucial in managing herbicide-resistant littleseed canarygrass.

Field studies were established in 1999 and 2000 to evaluate Italian ryegrass, wheat, and double-crop soybean response to fall and spring postemergence applications of flucarbazone, sulfosulfuron, clodinafop, diclofop, and tralkoxydim applied alone and in combination with thifensulfuron + tribenuron to winter wheat. Fall-applied herbicides caused 5% or less wheat injury. Spring-applied herbicides caused 3 to 45% wheat injury, and the greatest injury occurred with the combination of flucarbazone with thifensulfuron + tribenuron in the spring of 2001. Spring-applied sulfosulfuron, tralkoxydim, diclofop, and clodinafop caused 3 to 6% and 16 to 26% wheat injury in 2000 and 2001, respectively. Herbicide injury to wheat did not reduce wheat grain yield compared with the hand-weeded treatment. Italian ryegrass competition in the nontreated plots reduced wheat yield by as much as 33% compared with herbicide-treated plots. Italian ryegrass control was 89 to 99% from clodinafop and diclofop and 78 to 97% from flucarbazone, with no differences because of application timing in either year of the study. Italian ryegrass control from sulfosulfuron and tralkoxydim was greater from the spring of 2000 applications (94 to 99%) compared with the fall of 1999 applications (65 to 88%). However, in 2001, application timing (fall vs. spring) for sulfosulfuron and tralkoxydim did not affect Italian ryegrass control. Thifensulfuron + tribenuron combined with tralkoxydim reduced control of Italian ryegrass control compared with tralkoxydim alone in both years of the study. Italian ryegrass control was not reduced when thifensulfuron + tribenuron was combined with sulfosulfuron, flucarbazone, diclofop, or clodinafop. Italian ryegrass was controlled effectively by the acetyl-CoA carboxylase–inhibiting herbicides diclofop, clodinafop, and tralkoxydim. However, control of Italian ryegrass with the acetolactate synthase–inhibiting herbicides flucarbazone and sulfosulfuron was inconsistent. Double-crop soybean after wheat did not have foliar symptoms or yield loss from fall- or spring-applied herbicides.

A host-specific fungus Colletotrichum truncatum strain 00-3B1 (Ct) was mixed with herbicides to improve the control of scentless chamomile, a noxious weed in western Canada. The compatibility of Ct conidia (spores) with herbicides was evaluated in vitro, and varying effects were observed with different products on spore germination. Clodinafop, glufosinate, MCPA, and 2,4-D ester were relatively benign and delayed the germination slightly, whereas dicamba, imazethapyr, metribuzin, and 2,4-D amine were noticeably more inhibitive. Bromoxynil, glyphosate, sethoxydim, and Merge® (spray adjuvant) were most inhibitive, showing >50% inhibition after 24 h. To determine potential synergy, Ct was applied at 7 × 106 spores/ml in tank mixtures with selected herbicides at 1× and 0.1× registered rates under greenhouse conditions. Combining Ct with MCPA, 2,4-D ester, clopyralid, or metribuzin at 1× rate resulted in synergistic or additive interaction on scentless chamomile, increasing weed control significantly when compared to Ct or herbicides applied alone. Similar applications of Ct with imazethapyr, 2,4-D amine, dicamba, or glyphosate were antagonistic. Treatments with Ct plus 1× metribuzin killed scentless chamomile completely, whereas neither Ct nor the herbicide alone caused plant death, suggesting the value of this tank mixture.

Glyphosate-tolerant spring wheat currently is being developed and most likely will be the first major genetically engineered crop to be marketed and grown in several areas of the northern Great Plains of the United States. The public has expressed concerns about environmental risks from glyphosate-tolerant wheat. Replacement of traditional herbicide active ingredients with glyphosate in a glyphosate-tolerant spring wheat system may alter ecological risks associated with weed management. The objective of this study was to use a Tier 1 quantitative risk assessment methodology to compare ecological risks for 16 herbicide active ingredients used in spring wheat. The herbicide active ingredients included 2,4-D, bromoxynil, clodinafop, clopyralid, dicamba, fenoxaprop, flucarbazone, glyphosate, MCPA, metsulfuron, thifensulfuron, tralkoxydim, triallate, triasulfuron, tribenuron, and trifluralin. We compared the relative risks of these herbicides to glyphosate to provide an indication of the effect of glyphosate when it is used in a glyphosate-tolerant spring wheat system. Ecological receptors and effects evaluated were avian (acute dietary risk), wild mammal (acute dietary risk), aquatic vertebrates (acute risk), aquatic invertebrates (acute risk), aquatic plants (acute risk), nontarget terrestrial plants (seedling emergence and vegetative vigor), and groundwater exposure. Ecological risks were assessed by integrating toxicity and exposure, primarily using the risk quotient method. Ecological risks for the 15 herbicides relative to glyphosate were highly variable. For risks to duckweed, green algae, groundwater, and nontarget plant seedling emergence, glyphosate had less relative risk than most other active ingredients. The differences in relative risks were most pronounced when glyphosate was compared with herbicides currently widely used on spring wheat.

Acetyl-coenzyme A carboxylase (ACCase)–resistant Italian ryegrass is one of the most difficult-to-control weeds in United States wheat-production systems. Seed was collected from a suspected ACCase-resistant Italian ryegrass population in a winter wheat field with a history of ACCase-inhibitor herbicide use. This study investigated cross-resistance patterns in this Italian ryegrass population. Resistance was identified to the commercial dose of the ACCase herbicides pinoxaden, clethodim, sethoxydim, and clodinafop. Partial chloroplastic ACCase sequences revealed aspartate-to-glycine or isoleucine-to-asparagine substitutions at positions 2078 or 2041 in individuals of the resistant population. This is the first report, to our knowledge, of Asp-2078-Gly and Ile-2041-Asn substitutions in ACCase-resistant Italian ryegrass in the United States. Associating the occurrence of resistance alleles with resistance to specific active ingredients provides a better understanding of ACCase cross-resistance in Italian ryegrass and possibly options for its control.

Diclofop-resistant Italian ryegrass is a major weed problem in wheat production. This study aimed to determine the resistance pattern of diclofop-resistant Italian ryegrass accessions from the southern United States to the latest commercialized herbicides for wheat production, pinoxaden and mesosulfuron, and to other acetolactate synthase (ALS) and acetyl-CoA carboxylase (ACCase) inhibitors. Twenty-nine of 36 accessions were resistant to the commercial dose of diclofop. The majority (80%) of diclofop-resistant accessions were also resistant to clodinafop. Of 25 diclofop-resistant accessions, 5 were resistant to pinoxaden. All accessions tested were susceptible to the commercial dose of clethodim and sethoxydim. The cross-resistance pattern of diclofop-resistant Italian ryegrass to other ACCase inhibitors was 20% for pinoxaden and none with clethodim or sethoxydim. One accession was resistant to mesosulfuron but not to diclofop. This mesosulfuron-resistant accession was cross-resistant to sulfometuron but not to imazamox. All diclofop-resistant accessions tested were susceptible to ALS inhibitors, mesosulfuron, sulfometuron, and imazamox. Therefore, diclofop-resistant Italian ryegrass in Arkansas can be controlled with imazamox (in Clearfield wheat) and can mostly be controlled with mesosulfuron and pinoxaden. It could also be controlled by other selective grass herbicides in broadleaf crops.

Persistent use of herbicides has resulted in the selection of many herbicide-resistant weeds worldwide. A survey of 75 fields in the Palouse region of the inland Pacific Northwest was conducted to determine the extent of Italian ryegrass resistance to grass herbicides commonly used in winter wheat-cropping systems. Plants grown from collected seed samples were tested for resistance to diclofop, clodinafop, quizalofop, tralkoxydim, sethoxydim, clethodim, pinoxaden, triasulfuron, mesosulfuron, flucarbazone, imazamox, and flufenacet/metribuzin. Averaged across herbicide families within a herbicide group, some level of resistance was exhibited in 73, 31, and 31% of the populations to the aryloxyphenoxypropionates, cyclohexanediones, and phenylpyrazoline herbicides, respectively, and 39, 53, and 55% of the populations to the sulfonylureas, sulfonylaminocarbonyltriazolinone, and imidazolinone herbicides, respectively. Twelve percent of the populations showed some level of resistance to flufenacet/metribuzin. Cross-resistance to all acetyl coenzyme A carboxylase-inhibiting (group 1) herbicides was observed in 12% of the populations, whereas 25% of the populations were cross-resistant to all acetolactate synthase-inhibiting (group 2) herbicides tested. Of all the populations tested, 7% exhibited multiple resistance to at least one herbicide within all three groups tested. Only 5% of populations were completely susceptible to all 12 herbicides tested. These results indicate that herbicide-resistant Italian ryegrass populations are now common across much of the Palouse region in northern Idaho and eastern Washington.

Clodionafop, an acetyl-coenzyme A carboxylase (ACCase) inhibitor, changed the shape of the chlorophyll fluorescence induction curve (Kautsky curve) in barley and oat in greenhouse experiments. Biomass ED50, based on log-logistic dose–response curves, for barley was considerably higher than that for oat in all experiments. Biomass ED50 and relative potency (ED50 [barley]/ED50 [oat]) were consistent among experiments when sprayed at the same phenological stage of plant development. Especially at high doses, clodinafop changed the shape of the Kautsky curve more for oat than for barley. From the numerous parameters that can be derived from the OJIP steps of the Kautsky curve, we found that (1) F vj, the relative changes at the J step [F vj = (F m − F j)/F m], (2) area between Kautsky curve and maximum fluorescence (F m), and (3) F v/F m, maximum quantum efficiency of Photosystem II [F v/F m = (F m − F 0)/F m], were closely linked to the biomass dose–response relationships for both species. The linkage between biomass and the fluorescence parameters may be used to shorten the screening period for ACCase inhibitors.

A biotype of green foxtail found in Spain exhibited cross-resistance among acetyl-CoA carboxylase (ACCase)–inhibiting herbicides. Field doses that totally inhibited shoot fresh weight in the susceptible (S) biotype were determined for six aryloxyphenoxypropionates (clodinafop, diclofop, fenoxaprop-P, fluazifop-P, haloxyfop-P, and propaquizafop) and six cyclohexanediones (clefoxydim, clethodim, cycloxydim, sethoxydim, tepraloxydim, and tralkoxydim). The resistant (R) biotype showed cross-resistance to all herbicides except fenoxaprop-P, propaquizafop, clefoxydim, and tepraloxydim. There were no differences in the absorption, translocation, and metabolism of [14C]diclofop between the S and R biotypes. On the basis of herbicide dose that inhibited ACCase activity by 50% (I50 values), ACCase of the R biotype was 5.8-, 13.9-, 20.0-, 102.4-, 416.7-, and 625.0-fold less sensitive to clethodim, haloxyfop, diclofop, fluazifop, cycloxydim, and sethoxydim, respectively, than that of the S biotype. Two multifunctional ACCase isoforms (ACCase I and ACCase II) were purified partially and separated. ACCase II was highly resistant to diclofop acid in both biotypes, with I50 values ranging between 92 and 95 μM. However, the I50 values observed for ACCase I revealed that the R biotype was 30.8-fold less sensitive to diclofop than the S biotype. These results suggest the mechanism of resistance in green foxtail to diclofop relates to an altered ACCase I isoform.

Texas wheat producers have observed reduced efficacy and failure to control Italian ryegrass with registered rates of sulfonylurea herbicides that were previously effective. Growth chamber studies were conducted to quantify the sensitivity and distribution of Italian ryegrass ecotypes in Texas to triasulfuron and to determine alternative herbicide management options. Italian ryegrass seed samples were collected from over 40 wheat fields in 13 central and north Texas counties where declining Italian ryegrass control was reported by farmers following sulfonylurea herbicide application(s). Two-leaf Italian ryegrass was screened with an application of 150 g ai/ha triasulfuron, a rate five times the registered herbicide use rate. Sensitivity was determined by the response of an ecotype to that of a known susceptible population. Of the 48 Italian ryegrass ecotypes sampled, nine were comparable to susceptible standard, while the remaining 39 ecotypes were less sensitive to triasulfuron. Four of the least sensitive ecotypes to triasulfuron plus the susceptible standard were selected for a subsequent study. Diclofop, clodinafop, and metribuzin reduced fresh weights by at least 69, 71, and 62% across all ecotypes. No imazamox or triasulfuron treatment reduced fresh weights more than 60%.

Giant miscanthus is under consideration as a biofuel crop in the United States; however, there is little information on weed management for the establishment and survival of this crop. Therefore, greenhouse and field studies using ornamental pots were conducted in summer 2011 at Tifton, GA, with the objective of screening potential PPI, PRE, and POST herbicides and herbicide combinations for giant miscanthus when establishing from vegetative rhizomes. For the POST treatments, giant miscanthus was established from rhizomes in 7.6-L containers in the field and treated with 27 POST herbicides to evaluate efficacy. Thifensulfuron, metsulfuron, tribenuron, chlorimuron, halosulfuron, rimsulfuron, cloransulam, pinoxaden, bentazon, and metribuzin did not significantly lower shoot height, reduce shoot dry weight, or increase injury compared with nontreated control (NTC) when evaluated at 4 wk after treatment. Nicosulfuron, trifloxysulfuron, sulfometuron, clodinafop, fluazifop, and pyrithiobac caused the greatest injury, reduced plant height, and reduced dry weights compared with the NTC. Sethoxydim, diclofop, flumioxazin, imazamox, imazapic, and imazethapyr decreased plant heights or resulted in increased injury. PPI and PRE treatments included 21 herbicides and herbicide combinations applied at two rates. Results indicated that most treatments containing atrazine, metribuzin, pendimethalin, acetochlor, metolachlor, and mesotrione did not injure or stunt growth; however, EPTC at 4.5 kg ai ha−1 significantly reduced height and dry weight and oxadiazon resulted in greater injury compared with NTC at both rates. These results indicate that PPI, PRE, and POST herbicides can be utilized for establishment of giant miscanthus from vegetative rhizomes. Considering the invasive potential of giant miscanthus, several POST herbicides evaluated in this study such as fluazifop, pyrithiobac, and sulfometuron may be viable options to control this species if it becomes invasive.

Field experiments were conducted in Auburn, AL in 2008 and 2009 to assess new aryloxyphenoxypropionate (AOPP) herbicides applied alone and tank-mixed with triclopyr for Tifway bermudagrass control in Zorro zoysiagrass. Treatments included three sequential applications of clodinafop (0.07 kg ai ha−1); triclopyr (1.12 kg ae ha−1); clodinafop + triclopyr (0.07 + 1.12 kg ha−1); fenoxaprop (0.10 kg ai ha−1); fenoxaprop + triclopyr (0.10 + 1.12 kg ha−1); metamifop (0.40 kg ai ha−1); and metamifop + triclopyr (0.40 + 1.12 kg ha−1). Clodinafop, fenoxaprop, and metamifop applied alone controlled Tifway bermudagrass 32 to 65% when rated 3 wk after the final application; however, the addition of triclopyr to these AOPP herbicides increased bermudagrass control to ≥ 89%. All AOPP herbicides applied alone caused unacceptable injury to Zorro zoysiagrass (20 to 42%) 3 wk after the final application. Clodinafop resulted in the greatest zoysiagrass injury (42%), whereas metamifop caused moderate injury (20%). Zorro zoysiagrass exhibited less injury (< 3%) and greater turf coverage (≥ 95%) when AOPP herbicides were tank-mixed with triclopyr. Of the three AOPP herbicides evaluated in this study, only fenoxaprop is currently labeled for use on turfgrass. However, the nonlabeled herbicides (clodinafop and metamifop) provided bermudagrass control and zoysiagrass safety equal to the commercial standard (fenoxaprop) 3 wk after the final application when tank-mixed with triclopyr. Where zoysiagrass is contaminated with bermudagrass, turf managers will likely have to make applications of AOPP herbicides tank-mixed with triclopyr over multiple years to control bermudagrass as it will continue to regrow from deep rhizomes.

Field experiments were conducted at the Montana State University Southern Agricultural Research Center, Huntley, MT, in 2011 through 2013 to determine the effect of nitrogen (N) rate, seeding rate, and weed removal timing on weed interference in barley. A delay in weed removal timing from the 3- to 4-leaf (LF) stage to the 8- to 10-LF stage of barley resulted in up to 3.5-fold increase in total weed biomass and 10% reduction in barley biomass, and this was unaffected by a N rate that ranged from 56 (low) to 168 (high) kg ha−1. The effect of N rate on barley biomass was more pronounced when weed removal was delayed from the 3- to 4-LF stage to the 8- to 10-LF stage of barley and in nontreated plots. Increasing the barley seeding rate from 38 to 152 kg ha−1 increased the barley plant density by 50%, biomass by 13%, and grain yield by 29%, averaged over N rates and weed removal timing. On the basis of 5 and 10% levels of acceptable yield loss, the addition of ≥112 kg N ha−1 delayed the critical timing of weed removal by at least 1.3 wk in barley, compared with the 56 kg N ha−1 rate. A medium or high N rate prevented reduction in barley grain quality (plumpness and test weight) observed when the seeding rate was increased from 38 to 76 or 152 kg ha−1 at the low N rate. In a separate greenhouse study, the effect of N rate on the effectiveness of various herbicides for controlling wild oat, green foxtail, kochia, or Russian thistle was investigated. Results highlighted that wild oat or green foxtail grown under 56 kg N ha−1 (low N) soil required 1.4 to 2.6 times higher doses of clodinafop, fenoxaprop, flucarbazone, glyphosate, glufosinate, pinoxaden, or tralkoxydim for 50% reduction in shoot dry weights (GR50) compared with plants grown under 168 kg N ha−1 (high N). Similarly, a reduced efficacy of thifensulfuron methyl + tribenuron methyl, metsulfuron methyl, or bromoxynil+pyrasulfotole was observed (evident from the GR50 values) for kochia or Russian thistle grown under low- vs. high-N soil. Information gained from this research will aid in developing cost-effective, integrated weed management (IWM) strategies in cereals and in educating growers on the importance of fertilizer N management as a component of IWM programs.