Asynchronous flowering was noted in a recently discovered infestation of siam weed in north Queensland. This may indicate some genetic diversity in the infestation, increasing concerns about the origin of the infestation. Internal transcribed spacer 1 (ITS1) sequence data were obtained for siam weed individuals from north Queensland, Indonesia, Thailand, South Africa, Ivory Coast, Brazil, Colombia, and the U.S. The ITS1 region is 258 base pairs long, and the populations that flower at different times in north Queensland differ by four base substitutions. The genotype common in north Queensland is also reported throughout the native and introduced ranges. The other genotype is reported only in north Queensland and southern Brazil. These data, in conjunction with prior investigations into possible origins, indicate that Brazil is the most likely source of the infestation in Australia.

Two conventional greenhouses were modified into a containment facility for the study of foreign noxious weed species that could be dangerous to North American agriculture. The facility was designed for research on the biology, ecology, and weed control technology of foreign noxious weeds, without the escape of propagules. Two growth chambers and a germinator are located within the containment facility for evaluations under controlled environmental conditions. A standard operating procedure is followed to preclude the escape of weed seeds or propagules while minimizing restrictions to research. The facilities are locked and located in a secure fenced area to reduce the probability of accidental or intentional damage. Procedures for emergencies have been designed to facilitate aid without compromising the containment security. Knowledge concerning weeds that have the potential to threaten North American agriculture will be gained from research performed within this facility.