The carbon isotope distribution and its relationship with stable N and S isotope ratio values were investigated within a fish assemblage from the shallow lake Tapeliai, which is constantly affected by inflows of 14C depleted water from the surrounding watershed mires. The “conventional” radiocarbon age within the fish from this lake varied from 119 to 693 yr. The 14C/12C and δ13C values correlated significantly (r=0.85 p<0.001), which is not typical in lakes of the temperate zone. There were no observed statistical differences (Kruskal–Wallis ANOVA tests) in the 14C/12C values among different fish species. The radiocarbon dating values and 15N/14N measurements did not correlate. The radiocarbon measurement values also did not correlate with δ34S, however, the distribution of these isotopes in carp (119 yr and 1.3‰, respectively) and roach (344 yr and 4.5‰, respectively) indicated that fish may include allochthonous food sources in their diet.

Farming elements other than the crop, when integrated into the system, are supplementary, with multifold uses that include weed and pest management. Elements such as fish and poultry birds are integrated with transplanted wetland rice (Oryza sativa L.) for ensuring farmers’ livelihoods and the nutritional security and sustainability of the system. Integrated animal components such as poultry birds and fish also supplement the system with weed control. The role and efficacy of these animal components as tools for managing weedy rice (Oryza sativa L.) were explored, as weedy rice infestation is increasing. This threat of weedy rice is due to scarcity of water resulting from poor water management and improper field leveling. Grass carp (Ctenopharyngodon idella val.) produced the highest reduction of weedy rice biomass, 28% within 24 h under laboratory conditions. Polyculture of C. idella, mrigal (Cirrhinus mrigala Ham.), and silver carp (Hypophthalmichthys molitrix val.) reduced the biomass of weedy rice by 21% within 24 h. In laboratory studies, poultry manure at the highest concentration of 5% reduced the weedy rice seed germination 100% compared with rice seed germination at 91%. This conformed with microplot experiments in which poultry manure at 15.6 g d−1 resulted in a weed control index (WCI) of 8% in both years. However, poultry manure at 15.6 g d−1 in combination with herbicide application resulted in the highest control indices of weedy rice: 52% in 2017 and 2018. Integrating fish and poultry with PRE application of oxyfluorfen (0.25 kg ha−1) resulted in the highest WCI and grain yield in field experiments.

Parasite composition can be affected by physiological and ecological changes during host ontogeny. Intertidal fish do not travel long distances and live in the same area throughout their lifetimes, meaning that parasite communities can differ across geographic ranges. The objective of this study was to analyse the parasite communities of three fish species (Hypsoblennius sordidus, Helcogrammoides cunninghami and Scartichthys viridis) collected from the Chilean coast. The composition of parasite species was compared among host ontogenetic stages (larvae, juveniles and mature fish) and geographic areas. A total of 184 larval, 252 juveniles and 217 mature individuals were collected in the northern area (c. 24°S), and 186 larval, 192 juveniles and 112 mature individuals from the central area (c. 33°S). Ectoparasites were most prevalent in fish from the central area, whereas endoparasites were most prevalent in the northern area. The parasite species richness varied significantly between geographical areas for H. sordidus and H. cunninghami, but the parasite composition varied significantly between geographical areas for all fish species analysed. Therefore, the geographical area was the most important factor determining the parasite composition of intertidal fish species. The absence of endoparasites in fish larvae and the increased infestation in juvenile and mature fish may be explained by the shift in habitat from the water column to intertidal pools where prey abundance and availability are higher. On the other hand, hydrographic barriers affecting prey distributions may also offer an explanation as to the differences in parasite composition.

Stan Wood was an exceptional fossil collector who, over a collecting career of more than 40 years, provided British palaeontology with an abundance and variety of new Carboniferous fossils, the like of which had not been collected since Victorian times. So, what made him a great collector? Here, with the help of Stan's family, his friends and colleagues, we try to provide the answer. There is no single factor that stands out, but a complex mixture of innate and learned behaviours that together produced a unique talent. Although he acquired an Open University degree in geology, Stan was largely self-taught as a collector and, in doing so, became an accomplished and confident field geologist. He was naturally curious, persistent and very observant, with a photographic memory. He was tough, very strong and enjoyed hard physical work. He was congenial, unorthodox and a calculated risk taker. He asked questions, tested ideas and had a healthy disregard for authority. He was systematic, kept detailed records and shared his discoveries. He not only loved collecting fossils but, in the process, discovered in himself the essential qualities of a true scientist.

Stan Wood had a gift for finding exceptional Early Carboniferous fossils. Among them are 32 type specimens. His discoveries significantly changed our understanding of the history of life on Earth. Many of the fossils he collected are on display in museums across the UK and the localities he discovered continue to yield important new material. Here we briefly review some of Stan Wood's key achievements and describe the legacy he left.

Late glacial scenarios of ice retreat and biogeography databases constrain the dispersal routes of obligate freshwater fishes into Atlantic Canada and Maine. Evidence indicates glacial ice covered the present-day mainland and offshore islands at 18,000 14C yr before present. Possible refugia for extirpated freshwater fishes were the exposed outer edge of the Grand Banks (east), exposed Georges Bank (south-Atlantic Refugium), and the Mississippi Refugium in the west. It is improbable that the region was recolonized from the offshore refugia. Rather, fishes recolonized from the east via the upper St. Lawrence River valley into the upper Saint John River, Maine (Lake Madawaska) from 11,000 to 12,000 14C yr BP. The short period of entry resulted in the low diversity of obligate freshwater species in the region. Lake Madawaska was breached and dispersal continued into the remainder of the region after 8000 14C yr BP. By 6000 14C yr BP, access routes to the east along low-lying coastal zones were blocked by rising sea levels, which isolated Prince Edward Island, Cape Breton Island, and most probably Nova Scotia. Natural dispersal across the region appeared complete by this time.

A 25 m-thick outcrop section exposed at Torre Mucchia, on the sea-cliff north of Ortona, eastern central Italy, comprises a rare Middle Pleistocene succession of shallow-water and paralic sediments along the western Adriatic Sea. An integrated study of the section, including facies and microfacies analyses, and characterization of paleobiological associations (mollusks, fishes, ostracods, foraminifers and pollen), enable a detailed reconstruction of the paleoenvironmental and paleoclimatic conditions during deposition. The shallow-water deposits include a transgressive, deepening- and fining-upward shoreface to offshore-transition facies succession overlain by a regressive shoreface-foreshore sandstone body with an erosive base and a rooted and pedogenically altered horizon at the top that imply deposition during sea-level fall. This forced regressive unit is overlain by paralic strata forming a transgressive succession comprising palustrine carbonates and back-barrier lagoonal mudstones. The palustrine carbonates exhibit some of the typical features encountered in palustrine limestones deposited within seasonal freshwater wetlands (marl prairies). Following the sea-level rising trend, the freshwater marshes were abruptly replaced by a barrier-lagoon system that allowed deposition of the overlying mud-rich unit. Within these deposits, the faunal assemblages are consistent with a low-energy brackish environment characterized by a relatively high degree of confinement. The pollen record documents the development of open forest vegetation dominated by Pinus and accompanied by a number of mesophilous and thermophilous tree taxa, whose composition supports a tentative correlation with Marine Oxygen Isotope Stage 17. The new pollen record from Torre Mucchia improves our understanding of the vegetation development in the Italian Peninsula during the Middle Pleistocene and sheds new light on the role played by the most marked glacial periods in determining the history of tree taxa.

New records of fish species are reported from Papua New Guinea: Gymnothorax angusticauda (Muraenidae) from Madang, Coloconger scholesi Chan 1967 (Colocongridae) from Madang and New Guinea, Acromycter longipectoralis Karmovskaya 2004 (Congridae), Doryrhamphus melanopleura (Bleeker, 1858) and Festucalex gibbsi Dawson 1977 (Syngnathidae) from Madang, Lioscorpius longiceps Günther 1880 (Setarchidae) from Madang, Morobe and New Guinea, Neomerinthe megalepis Fowler 1938 and Pontinus rhodochrous (Günther 1872) (Scorpaenidae) from Madang and New Guinea, Parabembras curtus (Temminck & Schlegel 1843) (Parabembridae) from Morobe and New Guinea, Chrionema chryseres Gilbert 1905 and Pteropsaron levitoni Iwamoto 2014 (Percophidae) from Madang and New Guinea, Blenniella caudolineata (Günther, 1877) and Petroscirtes xestus Jordan & Seale, 1906 (Blenniidae) from Madang, Synchiropus orstom Fricke 2000 (Callionymidae) from West Sepik, Sandaun and New Guinea, Rexea antefurcata Parin 1989 (Gempylidae), Lepidoblepharon ophthalmolepis Weber 1913 (Citharidae), Engyprosopon macrolepis (Regan, 1908) (Bothidae), Aseraggodes kimurai Randall & Desoutter-Meniger 2007 (Soleidae), Cynoglossus bilineatus (Lacepède, 1802) and Symphurus multimaculatus Lee, Munroe & Chen 2009 (Cynoglossidae) from Madang and Tylerius spinosissimus (Regan 1908) (Tetraodontidae) from Morobe and Papua New Guinea. This papers also includes new depths records for Coloconger scholesi, Lioscorpius longiceps, Neomerinthe megalepis, Pontinus rhodochrous, Parabembras curtus, Chrionema chryseres and Synchiropus orstom.

New records of fish species are reported from New Caledonia, including Polyipnus aquavitus Baird, 1971 (Sternoptychidae), Porogadus melampeplus (Alcock, 1896) (Ophidiidae), Hoplichthys citrinus Gilbert 1905 (Hoplichthyidae), Plectrogenium nanum Gilbert 1905 (Plectrogeniidae), Lioscorpius trifasciatus Last, Yearsley & Motomura 2005 (Setarchidae), Neomerinthe megalepis Fowler 1938 and Phenacoscorpius megalops Fowler 1938 (Scorpaenidae), Ocosia apia Poss & Eschmeyer, 1975 (Tetrarogidae), Hoplostethus atlanticus Collett 1889 (Trachichthyidae), Zenion longipinnis Kotthaus, 1970 (Zeniontidae), Plectranthias foresti Fourmanoir 1977, Plectranthias pelicieri Randall & Shimizu, 1994, Plectranthias rubrifasciatus Fourmanoir & Randall, 1979 and Rabaulichthys squirei Randall & Walsh, 2010 (Serranidae), Synagrops philippinensis (Günther 1880) (Acropomatidae), Stegastes insularis Allen & Emery 1995 (Pomacentridae), Cirrhilabrus rubrimarginatus Randall 1992 (Labridae), Pteropsaron neocaledonicus Fourmanoir & Rivaton, 1979 (Percophidae), Centrodraco ornatus (Fourmanoir & Rivaton, 1979) and Draconetta xenica Jordan & Fowler 1903 (Draconettidae), and Acanthurus maculiceps (Ahl 1923) (Acanthuridae). A record of Plectropomus maculatus (Bloch 1790) (Serranidae) from Grande Terre, New Caledonia is confirmed. This paper also includes new depth records of Zenion longipinnis, Plectranthias rubrifasciatus, Synagrops philippinensis, Centrodraco ornatus and Draconetta xenica.

This paper deals largely with the dynamics and changes in the helminth parasite communities of fish along the trophic gradient of lakes. The use of parasitological community data as a bioindicator of environmental health underlines the need to study parasite communities at comparable localities with known pollution levels. The comparison of the conditions in different habitats might be helpful to differentiate between normal fluctuations in ambient conditions and pollution-mediated effects. Therefore, the present study was designed to examine the community structure of parasites in snow trout (Schizothorax niger Heckel) inhabiting 3 lakes of contrasting trophic status in Kashmir. The idea of selecting the lakes, namely Anchar (strongly hypereutrophic), Dal (eutrophic) and Manasbal (mesotrophic) for this study was intentional as they depict different trophic gradients and exhibit the desirable pattern which was a prerequisite for this study. The findings presented in this article suggest an apparent lake-wise gradient in community structure, as the increase in trematode and cestode infections in Anchar was markedly greater, to levels clearly distinguishable from those in the other two water bodies. We conclude that human-induced eutrophication of lakes modifies the parasite community at component level and community-level studies on parasites may provide information on health status of lakes.

The present study examined the effect of a catastrophic cyclone (Aila) on ichthyofaunal assemblages in a tidal river of the Sundarban Delta. Sampling in six stations with a gill-net of 20-m length and 1-cm mesh size resulted in the collection of 63 species in a pre-Aila survey. Among them, 16 species were not available in the year after the cyclone. However, 12 new species were added to the assemblages in the post-Aila year during which 59 species were recorded. Analysis of Similarity (ANOSIM) confirmed significant changes in fish assemblages after the cyclone with a corresponding reduction of the species diversity and variation in the seasonal pattern of abundance. Hydrological parameters also differed with a significant surge in nutrient concentrations. Tolerance to low dissolved oxygen seemed to be a determinant factor as evident from the higher abundance of certain fishes viz. Harpadon nehereus, Liza parsia, Pampus argentius, Tenualosa ilisha and Toxotes chatareus during post-Aila year. Despite the recovery of the ichthyofaunal assemblages at the later stage of the study, a strong seasonal variation was persistent. The study therefore suggests that environmental variation in terms of increasing temperature and salinity elicit greater response in an estuarine community than temporary natural disturbances even as severe as cyclones.

Food webs of streams draining tropical rain forests on Costa Rica's Pacific and Caribbean coasts were examined in the 1980s via dietary analyses, and the same streams were surveyed again in 2004 to compare trophic structure based on analysis of stable isotope ratios of fish, macro-invertebrate and plant tissues. Estimates of species’ trophic positions (TP) were calculated from stomach-contents data (51 species; 5420 specimens) and compared with TP estimates derived from analysis of nitrogen isotope ratios (82 taxa; 240 samples). Coefficients of determination for TP based on dietary versus isotopic analysis ranged from 0.18 (Quebrada Camaronal, Corcovado) to 0.73 (Quebrada Estacion, Tortuguero). Consumer taxa within all four streams spanned a broad range of carbon isotope values, indicating assimilation of variable proportions of carbon from periphyton and terrestrial vegetation that in all but one of the streams had similar δ13C values. Approximately half the consumers in all four streams had carbon ratios heavier than any of the in situ production sources examined. This pattern could be explained by consumption of other production sources that were not sampled, including periphyton taxa with variable carbon isotope signatures, or migration of prey and/or consumers between these freshwater and coastal marine habitats.

The taxonomy of trematodes of Great Barrier Reef (GBR) fishes has been studied in some detail for over 20 years. Understanding of the fauna has been informed iteratively by approaches to sampling, understanding of morphology, the advent of molecular methodology and a feed-back loop from the emergent understanding of host specificity. Here we analyse 658 host-parasite combinations for 290 trematode species, 152 genera and 28 families from GBR fishes. These are reported from 8 orders, 38 families, 117 genera and 243 species of fishes. Of the 290 species, only 4 (1·4%) have been reported from more than one order of fishes and just 23 (7·9%) infect more than one family; 77·9% of species are known from only one genus, and 60% from only one species of fish. Molecular studies have revealed several complexes of cryptic species and others are suspected; we conclude that no euryxenous host distribution should be accepted on the basis of morphology only. The occurrence of individual trematode species in potential hosts is patchy and difficult to predict reliably a priori or explain convincingly a posteriori. These observations point to the need for a vigorous iterative interaction between the accretion of host specificity data and its interpretation.

We explored the relationships between diet and morphology in 30 fish species from forested tropical streams of the Bolivian Amazon. These species were first assigned to eight broad trophic guilds based on stomach contents analysis. The relationships between diet and morphology were then examined using Redundancy Analysis, after having checked for potential phylogenetical effects. Results show that, independently of any phylogenetic constraints, some of the trophic guilds could be grossly predicted from few relevant morphological attributes (i.e. relative intestinal length, standard length and mouth orientation) and thus suggest a significant link between diet and morphology. In other words, species having similar diet tend to converge to some extent on some morphological attributes. This link was nevertheless rather weak, suggesting that even if morphology may set limits to patterns of resource use, these limits are broad enough to allow fishes changing their choice of prey resources to respond to local biotic and/or abiotic conditions.

An 8-week feeding trial was conducted to determine the dietary copper (Cu) requirement of juvenile hybrid tilapia, Oreochromis niloticus 5 O. aureus. Purified diets with eight levels (0, 1, 2, 3, 4, 8, 12 or 20 mg Cu per kg diet) of supplemental Cu were given to tilapia (mean initial weight 0·79±0·03 g). Each diet was given to three replicate groups of fish. The rearing water contained 0·98 µg Cu per l. Weight gain was highest in fish given diets supplemented with 2 mg Cu per kg diet, followed by the group given 1 mg Cu per kg diet, then the unsupplemented control group, and was lowest in the 20 mg Cu per kg diet group. The differences between each of these four groups were significant (P < 0·05). Food efficiency (FE) of fish showed a similar trend to that of weight gain. Blood haemoglobin (Hb) and haematocrit (Hct) concentrations were lowest in fish given ≥ 12 mg Cu per kg diet, followed by fish given the unsupplemented control diet and highest in fish given 2 mg Cu per kg diet (Hb) and 2 and 3 mg Cu per kg diet (Hct). Plasma ceruloplasmin activity was higher in fish given 1 and 2 mg Cu per kg diet, followed by 3 to 12 mg Cu per kg diet and the control group, and lowest in fish given 20 mg Cu per kg diet. The body Cu content in fish generally increased as the dietary Cu supplementation level increased. Analysis by broken-line regression of weight gain percent and by linear regression of the whole-body Cu retention of fish indicated that the adequate dietary Cu concentration in growing tilapia is about 4 mg Cu per kg diet.

To reach adult habitats, juveniles of three species of Hawaiian gobies (fishes under 3 cm long) climb waterfalls up to 350 m high, over 10 000 times their body length. The demands of moving through such an extreme environment could constrain the range of viable locomotor mechanisms that these fishes use. Previous qualitative observations indicated that Lentipes concolor and Awaous guamensis use ‘powerbursts’ of axial undulation to climb, whereas Sicyopterus stimpsoni ‘inches up’ vertical surfaces by alternately attaching oral and pelvic suckers to the substrate. To compare these propulsive mechanisms and their physiological requirements, high-speed video footage of climbing by juveniles from these three species on an artificial waterfall were collected, and climbing kinematics and performance for the two climbing styles were quantified. Bouts of powerburst climbing by L. concolor and A. guamensis typically begin in or near direct water flow and are initiated by a single, rapid adduction of the pectoral fins. Powerburst climbing bouts are rapid (12.4±1.0 body lengths (BL) s−1), but short in duration (0.07±0.02 s) with few continuous locomotor cycles (3.8±1.3 cycles bout−1). Powerburst climbers use high amplitude undulations along the entire body, but minimum resultant velocities of these undulations are high (>6 BL s−1). This suggests that these species may hybridize terrestrial propulsive mechanisms with aquatic mechanisms. In contrast, climbing by inching in S. stimpsoni involves little axial undulation or fin movement. Sicyopterus stimpsoni typically exit the water outside of direct flow and seem to use terrestrial propulsive mechanisms. As the oral disc attaches to the substrate, it expands to almost twice its resting area, after which the posterior body is pulled upwards; once the pelvic disc attaches, the oral disc releases and the anterior body advances. Climbing bouts include several continuous cycles of disc attachment (11.0±1.4 cycles bout−1) and last several seconds at velocities of 0.21±0.01 BL s−1. Before climbing waterfalls during migration to adult habitats, S. stimpsoni undergo a non-feeding metamorphosis that leads to the development of the mouth as a secondary locomotor organ. The unusual behaviour and ontogenetic strategy of S. stimpsoni seem to be evolutionary novelties, rather than ancestral retentions, suggesting that the evolution of these features may have been closely correlated. The substantial performance and kinematic distinctions between powerburst and inching climbing indicate that considerable locomotor diversity can evolve even in the context of extreme environmental demands.

Large rivers have played a prominent role in biogeographic theory for their potential to act as barriers for the dispersal of terrestrial organisms,and therefore be involved in the generation of species diversity (Brown & Lomolino 1998). In this paper, we document the potential role of macrophyte rafts as a mechanism by which Amazonian rivers could act as dispersal agents rather than barriers, transferring organisms across banks and possibly across very large distances. These vectors could therefore act against speciation and towards homogenization of the local biota.

Tropical marine protected areas (MPAs) may promote conditions that are attractive to dive tourists, but a systematic basis for assessing their effectiveness in this regard is currently lacking. We therefore interviewed 195 dive tourists in Jamaica to determine which reef attributes they most preferred to see on dives. Attributes relating to fishes and other large animals (‘big fishes’, ‘other large animals’, ‘variety of fishes’, ‘abundance of fishes’, and ‘unusual fishes’) were more appreciated than those relating to reef structure and benthos (‘reef structure e.g., drop-offs’, ‘variety of corals’, ‘large corals’, ‘coral cover’, ‘unusual corals’, ‘sponges’, ‘unusual algae’, ‘lobsters, crabs etc.’). We then surveyed reef condition with regard to those aspects (abundance and variety of fishes, number of ‘unusual’, and number of ‘large’ fish) at four Caribbean MPAs and reference areas. In two cases, Hol Chan Marine Reserve in Belize and Parque Nacional Punta Frances in Cuba, these fish attributes were more pronounced in the MPAs than in the reference areas. Differences between the Montego Bay Marine Park in Jamaica (MBMP) and adjacent reference areas were mainly restricted to shallow sites (<6m), while at Grand Cayman no differences between fully protected and partially protected areas were detected. Management had not been fully effective in the MBMP in the preceding months, while fishing pressure in the partially protected areas on Grand Cayman was very light. We conclude that, if fishing restrictions are well enforced, western Caribbean MPAs can be expected to be effective in ways appreciated by dive tourists.

The capability to detect and predict the responses of marine populations and communities to the establishment of marine protected areas (MPAs) depends on the ability to distinguish between the influences of management and natural variability due to the effects of factors other than protection. Thus, it is important to understand and quantify the magnitude and range of this natural variability at each scale of observation. Here we review the scale of responses of target populations and communities to protection within Mediterranean MPAs, against their ‘normal’ spatio-temporal heterogeneity, and compare those with documented cases from other temperate and tropical marine ecosystems. Additionally, we approach the problem of the relative importance of habitat structure, considered as a set of biological and physical elements of the seascape hierarchically arranged in space at multiple scales, to drive natural variability. We conclude that much more effort has to be made to characterize heterogeneity in relation to Mediterranean MPAs, and to quantify and explain relationships between target species and their habitats as sources of such variability. These studies should be based on sound sampling designs, which (1) generate long-term data sets, and would ideally (2) be based on a Mediterranean-wide comparison of a number of protected and unprotected localities, (3) be designed from a multi-scaled perspective, and (4) control for factors other than protection, in order to avoid their confounding effects. The need for appropriate spatial and temporal replication, nested designs and power analysis is advocated.