Smutgrass is a non-native perennial weed that is problematic because of its poor palatability to cattle and its difficulty to control once established. Limited literature exists to explain the effectiveness of herbicides other than hexazinone for smutgrass control and forage injury. This study aimed to evaluate seasonal applications of labeled herbicides used on forage for maximum smutgrass control. The second objective was to evaluate preemergent herbicides and hexazinone for their ability to control smutgrass germinating from seed. Hexazinone, nicosulfuron + metsulfuron-methyl, and glyphosate + imazapic were the most effective postemergence treatments, while quinclorac exhibited little activity on smutgrass. Common bermudagrass forage fully recovered from all treatments by 3 mo after treatment. Hexazinone, nicosulfuron + metsulfuron methyl, glyphosate, and imazapic were applied postemergence to smutgrass in spring, summer, and fall. Summer applications of hexazinone resulted in the greatest level of control, while spring treatments provided the least control. Applications of hexazinone or glyphosate resulted in the most effective smutgrass control. However, fall applications resulted in the least forage injury. Results of the study of preemergence herbicides indicate that treatments with indaziflam and hexazinone provide adequate control of germinating smutgrass seedlings in the greenhouse at 0.25×, 0.5×, and 0.75× of the lowest recommended labeled rate for seedling grass control. Indaziflam treatments prevented the emergence of any visible smutgrass seedling tissue, compared to hexazinone, which fully controlled the germinating seedlings by 21 d after treatment, whereas pendimethalin significantly reduced seedling numbers at the 0.5× and 0.75× rates.

Smutgrass is an invasive weed species that can quickly outcompete bahiagrass because of its aggressive growth, prolific seed production, and rhizomatous nature. Total renovation of bahiagrass pastures or hayfields is generally not a feasible or economically viable option for most producers. Therefore, controlling the continual spread of smutgrass will require an integrated weed management (IWM) plan that incorporates multiple strategies. The objective of this study was to test the interactions of herbicides and fertilizers on smutgrass control in bahiagrass and determine the most efficacious and economical IWM plan for low-input bahiagrass systems. This research was conducted on a mixture of ‘Tifton 9’ and ‘Pensacola’ bahiagrass at the Alapaha Beef Station in Alapaha, GA. The study design was a randomized complete block with a three-by-four factorial treatment arrangement with six replications. Fertility treatments included 56 kg N ha–1 (ammonium nitrate, 34% N) + 56 kg K2O ha–1, 56 kg N ha–1, and an unfertilized control. Smutgrass was reduced to <15% ground coverage when a postemergent herbicide was applied. The addition of a preemergent herbicide and/or fertilizer further reduced the coverage of smutgrass (P < 0.01). As smutgrass declined, the bahiagrass ground coverage increased; other vegetation and dead material did not differ by treatment. Generally, herbage accumulation and crude protein were only affected following the second N application (P < 0.01). Treatments that included preemergent (indaziflam) and postemergent (hexazinone) herbicides in addition to N and K2O resulted in an improved bahiagrass stand as timely weed suppression removed competition, while fertilizer provided essential nutrients for optimum growth to fill in the gaps. Combining herbicide and fertilizer is a more economical option for producers when compared to a complete bahiagrass renovation.

Pintoi peanut is a warm-season perennial legume that shows promise as a forage crop for the southeastern United States, however, little is known about the proper methods of weed management during establishment for this species. The objective of this study was to determine the ability of pintoi peanut to tolerate applications of PRE and POST herbicides during the year of and year after planting. The effects of herbicide treatments on percentage of visual estimates of injury and stand counts of pintoi peanut were investigated at Ona and Marianna, FL, in 2015 and 2016. All PRE herbicides did not result in significant injury or stand reduction. Pintoi peanut’s tolerance to POST herbicides was higher when plants were emerged for at least 2 wk prior to herbicide application. Stands of pintoi peanut that were planted the previous year appear to tolerate all herbicides examined in this work, except sulfosulfuron. Results of this study indicate that at the year of planting pintoi peanut is tolerant to PRE applications of pendimethalin, imazethapyr, and imazapic. Pintoi peanut appears to tolerate applications of 2,4-D, carfentrazone, imazapic and imazethapyr the year after planting at the rates utilized in this study. Future research should evaluate the effects of multiple herbicide applications and tank-mixes to obtain satisfactory weed control and selectivity in pintoi peanut swards.

This study investigated the effect of forage type (grass or red clover) and harvesting time (primary growth or regrowth) of silage on energy and N utilisation by sheep fed at maintenance level. Specifically, the assumption of constant loss of energy of digestible organic matter from energy losses in urine and CH4 applied in evaluation of silage metabolisable energy (ME) was investigated. Urinary excretion of high-energy phenolic compounds related to solubilisation of lignin was assumed to affect urinary energy (UE) losses from sheep fed highly digestible grass silage (GS). A total of 25 primary growth and regrowth silages of timothy (Phleum pratense) and meadow fescue (Festuca pratensis) grass mixtures and red clover (Trifolium pratense) samples collected in digestibility trials with sheep, including faecal and urine samples, were used for energy and N determinations. Urinary concentration of monophenolic compounds and CH4 emissions in vitro were also analysed. Daily faecal N output, CH4 yield (MJ/kg DM intake), proportion of CH4 energy in digestible energy (DE) and proportion of UE in DE were greater (P ≤ 0.03) in sheep fed red clover silage (RCS) than GS. Furthermore, less (P = 0.01) energy was lost as UE of DE in sheep fed primary growth GS compared with the other treatments. The relationship between UE and silage N intake or urinary N output for both silage types (i.e. grass v. red clover) was strong, but the fit of the regressions was better for GS than RCS. The CH4/DE ratio decreased (P < 0.05) and the UE/DE ratio increased (P < 0.05) with increasing organic matter digestibility in RCS. These relationships were not significant (P < 0.05) for the GS diets. The regression coefficient was higher (P < 0.05) for GS than RCS when regressing ME concentration on digestible organic matter. The results of this study imply that ME/DE ratio is not constant across first-cut GS of different maturities. The ME production response may be smaller from highly digestible first-cut GS but could not be clearly related to urinary excretion of monophenols derived from solubilisation of lignin. Furthermore, energy lost in urine was not clearly defined for RCS and was much more predictable for GS from silage N concentration.

The drive to increase the output of animal product in some sectors of ruminant livestock production has led to greater use of feeds such as cereal grains and soyabean meal that are potentially human-edible. This trend has caused concern since, by so doing, ruminants compete not only with monogastric livestock but also with the human population for a limited global area of cultivatable land on which to produce grain crops. Reasons for using potentially human-edible feeds in ruminant diets include increased total daily energy intake, greater supply of essential amino acids and improved ruminal balance between fermentable energy and degradable protein. Soyabean meal, produced on land that has been in arable cultivation for many years can fulfil a useful role as a supplier of undegraded dietary protein in diets for high-yielding dairy cows. However, in the context of sustaining the production of high-quality foods from livestock to meet the demands of a growing human population, the use of potentially human-edible feed resources by livestock should be restricted to livestock with the highest daily nutrient requirements; that is, potentially human-edible feed inputs should be constrained to meeting requirements for energy and protein and to rectifying imbalances in nutrient supply from pastures and forage crops such as high concentrations of nitrogen (N). There is therefore a role for human-edible feeds in milk production because forage-only systems are associated with relatively low output per head and also low N use efficiency compared with systems with greater reliance on human-edible feeds. Profitability on farm is driven by control of input costs as well as product value and examples are given of low-cost bovine milk and meat production with little or no reliance on potentially human-edible feeds. In beef production, the forage-only systems currently under detailed real-time life-cycle analysis at the North Wyke Farm Platform, can sustain high levels of animal growth at low feed cost. The potential of all-forage diets should be demonstrated for a wide range of ruminant milk and meat production systems. The challenge for the future development of ruminant systems is to ensure that potentially human-edible feeds, or preferably human-inedible by-products if available locally, are used to complement pastures and forage crops strategically rather than replace them.

Field trials were conducted in 2013 and 2014 to investigate the tolerance of limpograss to increasing rates of hexazinone. Dose-response curves were generated using linear and quadratic regression models to determine the hexazinone estimated dose (ED) required to provide 10% (ED10) and 20% (ED20) of visual injury and herbage mass reduction. The ED10 and ED20 for visual estimates of injury were estimated to be 0.05 and 0.14 kg ai ha-1 at 60 d after treatment (DAT). Regarding forage herbage mass reduction, the ED10 and ED20 were estimated to be 0.07 and 0.19 kg ai ha-1 in 2013, whereas in 2014, the ED10 and ED20 were estimated to be 0.03 and 0.06 kg ai ha-1, respectively. The significant difference in herbage mass reduction between 2013 and 2014 was likely due to rainfall patterns, which possibly promoted hexazinone leaching in 2013 and consequently, less activity. Overall, hexazinone resulted in high degrees of limpograss injury across all response variables in both years; therefore, smutgrass control in limpograss pastures with hexazinone may not be a viable option. The presence or absence of smutgrass should be considered before limpograss establishment as there is no viable herbicide to selectively remove smutgrass from limpograss swards.

Yields of birdsfoot trefoil (Lotus corniculatus L.) and red clover (Trifolium pratense L.) were increased during establishment after herbicide treatments. All treatments, which included preplanting application and incorporation of EPTC (S-ethyl dipropylthiocarbamate) at 2, and 4 kg/ha in all combinations with postemergence applications of dinoseb (2-sec-butyl-4,6-dinitrophenol) at 0.25 and 0.5 kg/ha, controlled weeds and increased yields over those of untreated plots during a 2-yr period. EPTC and dinoseb treatments alone and in combinations were economical in birdsfoot trefoil, using the cost for weed control and the value of the extra forage. However, with red clover only dinoseb proved economically feasible at hay prices less than $80/metric ton.

Dogfennel is one of the most problematic weeds in Florida pasturelands and its control can become inconsistent as the plant matures. A premix of triclopyr + fluroxypyr has been recently introduced for weed control in pastures and rangeland; however, little published information exists concerning the control of dogfennel in pastures with this herbicide combination. Therefore, experiments were initiated to determine the efficacy of triclopyr + fluroxypyr compared with commonly used pasture herbicides on dogfennel at three heights. All herbicides utilized in this study are commonly used for dogfennel control. Dogfennel control was affected by both herbicide treatment and dogfennel height. In general, 0.80 + 0.28 kg ai/ha of 2,4-D amine + dicamba resulted in inconsistent control, especially as dogfennel plants increased in size. Increasing the rate of 2,4-D amine + dicamba to 1.21 + 0.42 kg/ha increased the consistency. Triclopyr + fluroxypyr provided similar levels of control as that of 1.21 + 0.42 kg/ha 2,4-D amine + dicamba. In all locations, control of 154-cm dogfennel was signficanatly lower than that of 38-cm dogfennel. These data indicate that triclopyr + fluroxypyr is an effective option for dogfennel control, but dogfennel height at the time of application is an important factor for optimizing control.

The tolerance of bermudagrass and stargrass to the relatively new herbicide, aminocyclopyrachlor (ACP), must be known before it can be recommended for weed control in these forage systems. Field experiments were conducted in 2012 and 2013 in south-central Florida to determine the tolerance of established bermudagrass and stargrass to various rates and combinations of ACP, chlorsulfuron, 2,4-D, triclopyr, and metsulfuron. Overall, bermudagrass and stargrass injury was transient and was minimal by 60 d after treatment (DAT). Similarly, biomass production was negatively affected at 30 DAT when treated with ACP at rates of 70 g ae ha−1 or greater, but was similar to the nontreated control by 60 DAT. Tank-mixing ACP with chlorsulfuron, 2,4-D amine, triclopyr, or metsulfuron did not increase injury compared with ACP alone applied at equivalent rates. Forage nutritive values were unaffected by herbicides. These data suggest that long-term effects of ACP on bermudagrass and stargrass are negligible, and this herbicide could be an important component of weed management programs in these forage systems.

Field studies were used to examine the management strategies of mowing, herbicide, fertility, and all possible combinations on tall ironweed populations, weed biomass, and pasture yield at three Kentucky locations. Mowing was performed in July 2008 and 2009, herbicide was applied in August 2008, and fertilizer was applied in September 2008 and 2009 at all locations. Weed populations were measured in 2008, 2009, and 2010, and forage grass, clover, and weed biomass was collected in May or early June of 2009 and 2010. All treatments with herbicide reduced tall ironweed stems by 64% or greater in 2009 at all locations. Mowing alone, fertilizer alone, and mowing plus fertilizer did not reduce tall ironweed populations, except at one location where mowing alone reduced tall ironweed stems by 64% in 2009. Tall ironweed stems were not reduced in 2010 with any treatment at two locations, but herbicide combined with mowing or fertilizer reduced tall ironweed stems by 78% at the other location. Tall goldenrod population was reduced up to 100% by all treatments with herbicide or mowing alone, and mowing with fertilizer reduced tall goldenrod from 59 to 89%. Treatments did not reduce horsenettle populations. Herbicide-containing treatments reduced weed biomass at all locations. Weed biomass did not differ when comparing all treatments with and without mowing or treatments with or without fertilizer. Forage grass biomass was greatest with herbicide plus fertilizer and with the combination of mowing plus herbicide plus fertilizer at all locations in both years.

The objective of the present study was to estimate ruminal feed N outflow in lactating cows using the omasal sampling, compartmental flux or in situ method. A total of five ruminally fistulated Finnish Ayrshire dairy cows were used in a 5 × 5 Latin square study with 21 d periods. Experimental silages of grass or red clover harvested at two stages of maturity in addition to a supplement of 9·0 kg concentrate/d were fed to the cows. In vivo omasal N flow was determined using the omasal sampling technique. Ruminal in situ N flow was calculated from N intake and degradability (38 μm nylon bags). The samples of ruminal contents and faeces were divided into seven particle-size fractions by wet sieving; the concentrations of indigestible neutral-detergent fibre and N were used to calculate N flow in the compartmental flux method. In vivo omasal N flow was greater for the red clover silage diets than for the grass silage diets. The N flow calculated using the compartmental flux technique and that calculated using the in situ technique were highly correlated, but both were less than and poorly correlated with the in vivo N flow. In both in situ and compartmental flux techniques, forage maturity increased the particle-associated N flow, with the increase being significantly greater for the red clover diets than for the grass silage diets. In conclusion, the compartmental flux and in situ methods described the N flow associated with the particle fractions rather than the total ruminal outflow of feed N.

The feeding value of forage mixtures from permanent and temporary multi-species grasslands cannot always be precisely defined. Indeed, the digestibility and feed intake of a combination of forages can differ from the balanced median values calculated from forages considered separately. In order to present an overview of the associative effects between forages on digestion and intake, a literature study was carried out. The associative effects can be studied in a complementary way in vitro to test digestive interactions of a large number of mixtures and to carry out explanatory experiments, and in vivo to investigate intake and digestion at the whole animal scale. We identified three main situations in which interactions between forages can lead to associative effects on intake and digestion: (i) increased intake that can be observed with grass and legume association can be explained by fast digestion of the soluble fraction of legumes, and a higher rate of particle breakdown and passage through the rumen, (ii) increased digestion when a poor forage is supplemented by a high nitrogen content plant can be explained by stimulation of the microbial activity and (iii) modification of digestive processes in the rumen, including proteolysis and methane production when certain bioactive secondary metabolites such as tannins, saponins or polyphenol oxidase are present. According to the type and concentration of these compounds in the diet, the effects can be favourable or unfavourable on intake and digestive parameters. Reported associative effects between forages show a large variability among studies. This reflects the complexity and multiplicity of nutritional situations affecting intake and the rumen function in a given animal. In order to provide more reliable information, further accumulation of data combining in vitro and in vivo studies is required. A better understanding of the associative effects between forages could help to optimise feed use efficiency, resulting in greater productivity, a reduction of the environmental impact of animal emissions and more sustainable animal production.

Even though extensive research has examined the role of nutrition on milk fat composition, there is less information on the impact of forages on milk fatty acid (FA) composition. In the current study, the effect of replacing grass silage (GS) with maize silage (MS) as part of a total mixed ration on animal performance and milk FA composition was examined using eight multiparous mid-lactation cows in a replicated 4 × 4 Latin square with 28-day experimental periods. Four treatments comprised the stepwise replacement of GS with MS (0, 160, 334 and 500 g/kg dry matter (DM)) in diets containing a 54 : 46 forage : concentrate ratio on a DM basis. Replacing GS with MS increased (P < 0.001) the DM intake, milk yield and milk protein content. Incremental replacement of GS with MS in the diet enhanced linearly (P < 0.001) the proportions of 6:0–14:0, decreased (P < 0.01) the 16:0 concentrations, but had no effect on the total milk fat saturated fatty acid content. Inclusion of MS altered the distribution of trans-18:1 isomers and enhanced (P < 0.05) total trans monounsaturated fatty acid and total conjugated linoleic acid content. Milk total n-3 polyunsaturated fatty acid (PUFA) content decreased with higher amounts of MS in the diet and n-6 PUFA concentration increased, leading to an elevated n-6 : n-3 PUFA ratio. Despite some beneficial changes associated with the replacement of GS with MS, the overall effects on milk FA composition would not be expected to substantially improve long-term human health. However, the role of forages on milk fat composition must also be balanced against the increases in total milk and protein yield on diets containing higher proportions of MS.

The transition away from forage-based cropping systems in Iowa to corn and soybean rotations since World War II has corresponded with degraded economic and environmental conditions in the state. Falling net incomes for farmers and concern over global warming and the effects of agriculture-related pollution on water, wildlife and human health have increased interest in diversified cropping systems. This paper reviews the benefits of diversifying Iowa corn and soybean rotations with perennial forage species such as alfalfa and red clover. Perennial forage crops improve soil quality, decrease NO3-N leaching and soil erosion, increase carbon sequestration and decrease pesticide and herbicide needs by controlling weed and insect pests. Forage legumes reduce N fertilizer needs for succeeding corn crops at a higher rate than soybeans, and corn crops following forages have higher yields than after corn or soybeans. Farmers who add alfalfa to corn and soybean rotations could realize significant economic gains. A simulated 5-year rotation in Iowa including corn–soybeans–oats/alfalfa–alfalfa–alfalfa would result in a 24% net income increase over 5 years of corn–soybean–corn–soybean–corn, even with government farm support payments for the row crops. Farm policies that encourage commodity production create little incentive for Iowa farmers to diversify their cropping systems beyond corn and soybeans, despite the clear economic and ecological benefits. We recommend increasing federal support for conservation programs that reward environmentally beneficial farm practices such as the Conservation Securities Program and we encourage land grant universities to hire researchers interested in alternative agricultural systems.

The aim of this study was to evaluate the sulphur hexafluoride (SF6) tracer technique for methane (CH4) emission measurement in sheep. Ten cryptorchid Romney sheep were involved in two indoor trials (T1 and T2), where daily CH4 emissions were individually measured both by the SF6 tracer (‘tracer CH4’) and by the indirect calorimetry chamber (‘chamber CH4’) techniques while fed on lucerne hay at 1.2 times maintenance requirements. Separate sets of permeation tubes with pre-calibrated permeation rates (‘pre-calibrated PRs’) were used in the two trials (for tracer CH4) and at the time of T1 and T2 these tubes had been deployed in the rumen for 250 and 30 days, respectively. The tracer CH4 measurements were carried out for 2 (T1) and 5 (T2) days in digestibility crates housed within a building (T1) or a well-ventilated covered yard (T2). Sheep were transferred to calorimetry chambers for 3 days acclimatisation, followed by measurement of CH4 emission for 7 (T1) and 3 (T2) days. In T1 samples from the chamber, outflow and inflow (collected over ∼22 h) were analysed for CH4 and SF6 concentrations using the tracer protocol. Thus, PRs of SF6 at the time of the trials (‘calculated PRs’) could be inferred and the corresponding CH4 emissions are then calculated using either the pre-calibrated PR or calculated PR. Permeation tubes were recovered at the end of the animal trials and their ‘post-recovery PR’ determined. In trial T1, the tracer CH4 estimates (based on the pre-calibrated PR) were much higher and more variable than the chamber CH4 values. In this trial, the calculated PR and the post-recovery PR were similar from each other but smaller than the pre-calibrated PR, and when the calculated PR was used in place of the pre-calibrated PR the CH4 emission estimates agreed well with the chamber CH4 values. This suggested that the discrepancy was due to a declining PR during the long deployment time of the tubes in T1, an observation reported elsewhere. When the long intra-ruminal deployment was avoided in T2, good agreement between the techniques for CH4 emission measurement was observed.

Two experiments were performed with post-valve T-cannulated growing pigs, using five animals in each experiment in a change-over design to evaluate the effect of inclusion of four different dried forage meals on ileal crude protein (CP) and amino acid (AA) digestibilities. The control diets (C1 and C2) were barley-based and the experimental diets were formulated by replacing the barley with 100 or 200 g/kg of either lucerne (Medicago sativa) or white clover (Trifolium repens) meal in Expt 1 and red clover (Trifolium pratense) or perennial ryegrass (Lolium perenne) meal in Expt 2. A decrease (P < 0·05) in the apparent ileal digestibility of CP and most of the essential and nonessential AA was found with the inclusion of lucerne, white clover and perennial ryegrass meal in the barley-based diets. When red clover meal was included, only the apparent ileal digestibilities of CP, leucine, phenylalanine, tyrosine and glutamic acid were found to decrease (P < 0·05). The estimated apparent ileal digestibilities of most essential AA in the forage meals were lower than in the barley-based diets. The ileal flow of glucosamine and ornithine was found to increase (P < 0·05) with increasing proportion of fibre in the diet, suggesting an increase in endogenous N secretions and small-intestinal microbial activity. With the minor changes found for ileal essential AA digestibilities with forage meal inclusion in the diet the present data confirm the potential of forage meals as a source of protein in pig diets.