Non-alcoholic fatty liver disease (NAFLD) includes a range of disorders from simple steatosis to non-alcoholic steatohepatitis. There is no proven drug treatment for NAFLD, and diet modification is considered part of the main line of treatment for this disease. The aim of this study was to investigate the efficacy of garlic supplementation in NAFLD patients. The effect of garlic powder supplementation on hepatic steatosis, liver enzymes and lipid profile was investigated in NAFLD patients. Ninety NAFLD patients were randomly assigned to take either a garlic powder supplement or a placebo for 12 weeks. The treatment group received four tablets of garlic daily (each tablet contained 400 mg garlic powder). The control group received four tablets of placebo (each placebo contained 400 mg starch). At the end of the study, hepatic steatosis was significantly reduced in the treatment group compared with the control group (P = 0·001). In addition, a significant decrease was seen in the serum concentration of alanine transaminase (P < 0·001), aspartate transaminase (P = 0·002), γ-glutamyltransferase (P = 0·003) as well as total cholesterol (P = 0·009), TAG (P < 0·001), HDL-cholesterol (P < 0·001) and LDL-cholesterol (P = 0·01) in the treatment group compared with the control group. No significant difference was seen between the two groups in serum concentration of alkaline phosphatase. Overall, garlic powder supplementation improved hepatic features and lipid profile among NAFLD patients.

Field studies were conducted in 1991 and 1992 at the Faculty Farm of the U.A.E. Univ., Al-Ain, to evaluate the effectiveness of soil solarization for weed management in naturally infested plots of lettuce, and on subsequent planting of garlic. Solarization for 2 mo during the hottest summer period effectively suppressed weeds during the growing season of head lettuce. In subsequently-planted garlic, no significant effect was observed between solarized and nonsolarized treatments.

The aim of this review paper is to show the most important bioactive compounds of garlic (Allium sativum L.), modes of action of garlic powder and its effects on broiler chicken productive performance. Allicin is thought to be the principal bioactive compound present in aqueous garlic extract or raw garlic homogenate. When garlic is chopped or crushed, the allinase enzyme present in garlic is activated and acts on alliin to produce allicin. Other important sulphur containing compounds present in garlic are allyl methyl thiosulphonate, 1-propenyl allyl thiosulphonate and γ-L-glutamyl-S-alkyl-L-cysteine. These active compounds are thought to influence feed consumption, feed utilisation, body weight, blood lipid profiles and cholesterol levels in chickens. Biological responses to these bioactive compounds have been attributed to a reduction in risk factors for cardiovascular diseases and cancer, stimulation of immune function, enhanced detoxification of foreign compound, hepatoprotection, antimicrobial, antifungal, anti-inflammatory and antioxidant effects.

During the past fifteen years, phytoadditives in animal nutrition have attracted attention for their potential role as alternatives to antibiotic growth promoters. The goal of this paper is to review the current scientific data on the use of phytoadditives in broiler nutrition. The efficacy of phytogenic applications in broiler nutrition depends on several factors, such as composition and feed inclusion level of phytogenic preparations, bird genetics, and overall diet composition. Difficulty arises when comparing different studies using phytoadditives due to the large variation in composition and sourcing, thus the potential biological effects of phytogenic compounds may differ. However, a great amount of research data supports a potential role of phytoadditives as natural, non-antibiotic growth promoters in broiler nutrition. The mechanisms behind growth promotion are far from being elucidated, as data on phytoadditive effects on nutrient digestibility, gut function and the immune system are still scarce. Phytogenic intake may certainly depress pathogen growth in the gut, however an understanding of their effects on the complex gut ecosystem is still far from clear. There is insufficient amount of studies describing the effects of phytoadditive dietary intake on carcass meat safety, whereas the beneficial effect of phytogenics on carcass meat quality is very well documented. As the last point of this review, further considerations on the efficient applications of phytoadditive compounds in broiler nutrition are given.

Poultry researchers and nutritionists are looking for viable alternative feed additives since conventional supplements have been criticised for their potential negative impact on the food chain. Among the currently available poultry feed additives, natural herbs and plants have been widely advocated due to their reported widespread beneficial effects. Garlic (Allium sativum) is one such potential feed supplement which has recently been reported as having a wide range of beneficial effects on the production performance and physiological biochemistry of broilers and laying hens. Notable beneficial effects have been seen on growth, feed efficiency, egg production and quality, as well as stimulation of immune system and lowering blood cholesterol levels in poultry birds. The results reported vary from author to author probably due to variations in the dose of the product fed, the duration of feeding and processing techniques employed.

The effects of a compound including the secondary metabolites of garlic, propyl thiosulphinate (PTS) and propyl thiosulphinate oxide (PTSO), on the in vitro and in vivo parameters of chicken gut immunity during experimental Eimeria acervulina infection were evaluated. In in vitro assays, the compound comprised of PTSO (67 %) and PTS (33 %) dose-dependently killed invasive E. acervulina sporozoites and stimulated higher spleen cell proliferation. Broiler chickens continuously fed from hatch with PTSO/PTS compound-supplemented diet and orally challenged with live E. acervulina oocysts had increased body weight gain, decreased faecal oocyst excretion and greater E. acervulina profilin antibody responses, compared with chickens fed a non-supplemented diet. Differential gene expression by microarray hybridisation identified 1227 transcripts whose levels were significantly altered in the intestinal lymphocytes of PTSO/PTS-fed birds compared with non-supplemented controls (552 up-regulated, 675 down-regulated). Biological pathway analysis identified the altered transcripts as belonging to the categories ‘Disease and Disorder’ and ‘Physiological System Development and Function’. In the former category, the most significant function identified was ‘Inflammatory Response’, while the most significant function in the latter category was ‘Cardiovascular System Development and Function’. This new information documents the immunologic and genomic changes that occur in chickens following PTSO/PTS dietary supplementation, which are relevant to protective immunity during avian coccidiosis.

The present article reviews the historical and popular uses of garlic, its antioxidant, haematological, antimicrobial, hepatoprotective and antineoplastic properties and its potential toxicity (from sulfoxide). Garlic has been suggested to affect several cardiovascular risk factors. It has also been shown that garlic and its organic allyl sulfur components are effective inhibitors of the cancer process. Since garlic and its constituents can suppress carcinogen formation, bioactivation and tumour proliferation, it is imperative that biomarkers be established to identify which individuals might benefit most. Garlic powder, aged garlic and garlic oil have demonstrated antiplatelet and anticoagulant effects by interfering with cyclo-oxygenase-mediated thromboxane synthesis. Garlic has also been found to have synergistic effects against Helicobacter pylori with a proton pump inhibitor. The active compound allicin may affect atherosclerosis not only by acting as an antioxidant, but also by other mechanisms, such as lipoprotein modification and inhibition of LDL uptake and degradation by macrophages. Freshly prepared garlic homogenate protects against isoniazid+rifampicin-induced liver injury in experimental animal models. Several mechanisms are likely to account for this protection.

In order to determine the impact of garlic on total cholesterol (TC), TAG levels, as well as LDL and HDL, and establish if any variables have an impact on the magnitude of this effect, a meta-analysis was conducted. A systematic literature search of MEDLINE, CINAHL and the Cochrane Database from the earliest possible date through to November 2007 was conducted to identify randomised, placebo-controlled trials of garlic that reported effects on TC, TAG concentrations, LDL or HDL. The weighted mean difference of the change from baseline (with 95 % CI) was calculated as the difference between the means in the garlic groups and the control groups using a random-effects model. Subgroup and sensitivity analyses were performed to determine the effects on type, brand and duration of garlic therapy as well as baseline TC and TAG levels, the use of dietary modification, and study quality on the meta-analysis's conclusions. Twenty-nine trials were included in the analysis. Upon meta-analysis garlic was found to significantly reduce TC ( − 0·19; 95 % CI − 0·33, − 0·06 mmol/l) and TAG ( − 0·11; 95 % CI − 0·19, − 0·06 mmol/l) but exhibited no significant effect on LDL or HDL. There was a moderate degree of statistical heterogeneity for the TC and TAG analyses. Garlic reduces TC to a modest extent, an effect driven mostly by the modest reductions in TAG, without appreciable LDL lowering or HDL elevation. Higher baseline line TC levels and the use of dietary modification may alter the effect of garlic on these parameters. Future studies should be conducted evaluating the impact of adjunctive garlic therapy with fibrates or statins on TAG concentrations.

Funeral pyres identified at a fourth-century BC cemetery on Thasos have produced a range of plants. The authors show that strongly represented among them are pomegranate, garlic and grape, as well as bread – foodstuffs for funeral feasts and with significance for religious practice.