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Chapter 8 - Tests of Sperm-Cervical Mucus Interaction
David Mortimer, Lars Björndahl, Karolinska Institutet, Stockholm, Christopher L. R. Barratt, University of Dundee, José Antonio Castilla, Roelof Menkveld, Stellenbosch University, South Africa, Ulrik Kvist, Karolinska Institutet, Stockholm, Juan G. Alvarez, Trine B. Haugen
Book:

A Practical Guide to Basic Laboratory Andrology

Published online:

16 February 2022

Print publication:

10 March 2022, pp 175-193
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Summary

Sampling of cervical mucus, its assessment, and the investigation of sperm-mucus interaction are described in detail. Methods are provided for a range of common tests that are structured as standard operating procedures (SOPs) for easy use at the bench. Methods are focussed on objectivity, robustness, standardized reporting, controlling the risk of errors, and minimizing measurement uncertainty. Includes the post-coital test, Kurzrok-Miller slide test, the Kremer capillary tube sperm penetration test, and the sperm-cervical mucus contact sldie test. An explanation of crosed hostility testing is also included, along with a discussion of complementary tests and the use of cervical mucus surrogates.