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Chapter 8 - Tests of Sperm-Cervical Mucus Interaction

Published online by Cambridge University Press:  16 February 2022

David Mortimer ,
Lars Björndahl ,
Christopher L. R. Barratt ,
José Antonio Castilla ,
Roelof Menkveld ,
Ulrik Kvist ,
Juan G. Alvarez  and
Trine B. Haugen
David Mortimer
Affiliation:
Oozoa Biomedical Inc., Vancouver
Lars Björndahl
Affiliation:
Karolinska Institutet, Stockholm
Christopher L. R. Barratt
Affiliation:
University of Dundee
José Antonio Castilla
Affiliation:
HU Virgen de las Nieves, Granada
Roelof Menkveld
Affiliation:
Stellenbosch University, South Africa
Ulrik Kvist
Affiliation:
Karolinska Institutet, Stockholm
Juan G. Alvarez
Affiliation:
Centro ANDROGEN, La Coruña
Trine B. Haugen
Affiliation:
Oslo Metropolitan University
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Summary

Sampling of cervical mucus, its assessment, and the investigation of sperm-mucus interaction are described in detail. Methods are provided for a range of common tests that are structured as standard operating procedures (SOPs) for easy use at the bench. Methods are focussed on objectivity, robustness, standardized reporting, controlling the risk of errors, and minimizing measurement uncertainty. Includes the post-coital test, Kurzrok-Miller slide test, the Kremer capillary tube sperm penetration test, and the sperm-cervical mucus contact sldie test. An explanation of crosed hostility testing is also included, along with a discussion of complementary tests and the use of cervical mucus surrogates.

Keywords

cervical mucusInsler scoresperm-mucus interactionsperm transportpost-coital test
Type
Chapter
Information
A Practical Guide to Basic Laboratory Andrology , pp. 175 - 193
Publisher: Cambridge University Press
Print publication year: 2022

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References

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