The adoption of conservation tillage systems has been challenged by concerns potential weed species shifts. A 9-yr study from 1988 to 1996 was conducted Delhi, Ontario, on a loamy sand soil to evaluate the effect of tillage systems (conventional [CT] and no-till [NT]), cover crop Secale cereale, and nitrogen (N) rate (0, 50, 100, 125, 150, and 200 kg N ha−1) on monocrop Zea mays L. (corn) yield and changes in the composition of the weed flora. CT consisted of spring moldboard plowing followed by cultivation with a tooth cultivator. Weed counts were taken in the last 3 yr of the study (1994, 1995, and 1996) prior to postemergence herbicide application and then again 2 to 3 wk after herbicide treatment. Composition or the weed flora was analyzed by canonical discriminant analysis (CDA). The relationship between weed density and tillage system was not consistent. Weed species composition differed between CT and NT systems. Chenopodium album and Amaranthus retroflexus were associated with CT and Digitaria sanguinalis with NT. N rate and cover crop did not affect weed density or species composition. Proper management of weeds with herbicides appeared to minimize any long-term effect on the weed flora resulting from varying N rates. Zea mays yields did not differ between CT and NT systems but were greater in both systems with a cover crop at the higher N rates. Disturbance caused by tillage was more important than N rate and cover crop as a mechanism influencing composition of the weed flora.