Two sediment sections are investigated at Cape Arkhangelos, island of Rhodes, where Pleistocene marine sediments crop out in horsts and grabens of a Mesozoic basement. There, hemipelagic sediments characterized by upper bathyal communities are atypically mixed with much shallower faunal components because they were deposited close to rugged coastal landforms. Biostratigraphic analyses show that the sections were deposited between 1.8 and 0.9 Ma, and between 1.8 and 1.6 Ma, respectively. By combining the planktonic/benthic foraminiferal ratio with 31 bathymetric indicators chosen among extant species of benthic foraminifera, mollusks, and bryozoans, we show that relative sea-level fluctuations can be reconstructed in these atypical settings despite the proximity of steep slopes that favored transportation of allochthonous fauna. The shallow-water components (including gravels and calcareous algae) were transported downslope by the combined action of gravity, currents, and tectonic disturbance that promoted drowning (with a maximum flooding recorded at ca. 1.7 Ma) and then uplift of fault-bounded paleovalleys that formed during the Early Pleistocene. Abrupt facies changes and age differences between sections have been triggered by the irregular paleotopography of the Mesozoic basement, which fostered differential depositional settings, with outer to middle neritic deposits above the horsts and upper bathyal deposits in paleovalleys.

Late Quaternary fluvial channel deposits are notoriously difficult to date. In the midwestern United States, shells of aquatic mollusks can be found within many fluvial channel sediments and therefore can be radiocarbon (14C) dated to determine the age of the deposits. However, carbonate platform rocks are abundant in this region, potentially causing freshwater 14C reservoir effects (FRE) in mollusk shells. We 14C dated 11 aquatic gastropod and bivalve shell samples from specimens collected live from a stream in southwestern Ohio during three different years to assess the modern 14C reservoir effect. Modern samples yielded an average 14C FREmodern of 518 ± 65 14C yrs for 2020 (n=5), 640 ± 34 14C yrs for 2021 (n=2), and 707 ± 76 14C yrs for 2022 (n=4). We also 14C dated matched pairs of organic wood or charcoal and aquatic mollusk shells from late Pleistocene and Holocene deposits in the Four Mile Creek floodplain to determine the FREfossil. These samples, free of any potential influence from nuclear bomb testing, yielded an overall weighted mean FREfossil of 1029 ± 345 14C yrs. We then assess the advantages and limitations of both the FREmodern and FREfossil methods for determining freshwater reservoir effects. Finally, we apply the FREfossil correction to a series of shell ages from fluvial terrace deposits as a case study. The results indicate that although there is a 14C FRE in streams from the midwestern United States, aquatic shells can provide robust age control on fluvial channel deposits. More research is needed to understand the spatial and temporal variability of FREs, as well as any species effects, among various watersheds across the midwestern United States.

Studies of pre-bomb mollusks live-collected around the Australian coastline have concluded that near-shore marine radiocarbon reservoir effects are small and relatively uniform. These studies are based on limited samples of sometimes dubious quality representing only selective parts of Australia’s lengthy coastline. We systematically examine spatial variability in the marine radiocarbon reservoir effect (ΔR) through analysis of 292 live-collected mollusk samples across the Australian mainland coasts and near-shore islands subject to strict selection criteria. This study presents 233 new ΔR values combined with an evaluation of 59 previously published values. Results demonstrate significant spatial variability in marine radiocarbon reservoir effects across the study region. ΔR values range from 68 ± 24 14C years off the Pilbara region of Western Australia to –337 ± 46 14C years in the southern Gulf of Carpentaria in Queensland. Most sets of local values exhibit internal consistency, reflecting the dominant influence of regional oceanography, including depletion in ΔR values southwards along the eastern Australian coastline coincident with the East Australian Current. Anomalous values are attributed to inaccurate documentation, species-specific relationships with the carbon cycle and/or short-term fluctuations in marine radiocarbon activities. To account for the heterogeneous distribution of marine 14C, we recommend using a location specific ΔR value calculated using the Australian ΔR Calculator, available at: https://delta-r-calc.jcu.io/.

Perkinsus olseni is an industrially significant protozoan parasite of Manila clam, Ruditapes philippinarum. So far, various media, based on Dulbecco's Modified Eagle Medium and Ham's F-12 nutrient mixture with supplementation of fetal bovine serum (FBS), have been developed to proliferate the parasitizing trophozoite stage of P. olseni. The present study showed that P. olseni did not proliferate in FBS-deficient Perkinsus broth medium (PBMΔF), but proliferated well in PBMΔF supplemented with tissue extract of host Manila clams, indicating that FBS and Manila clam tissue contained molecule(s) required for P. olseni proliferation. Preliminary characterization suggested that the host-derived molecule(s) was a heat-stable molecule(s) with a molecular weight of less than 3 kDa, and finally a single molecule required for the proliferation was purified by high-performance liquid chromatography processes. High-resolution electrospray ionization mass spectrometry and nuclear magnetic resonance analyses identified this molecule as glycine betaine (=trimethylglycine), and the requirement of this molecule for P. olsseni proliferation was confirmed by an assay using chemically synthesized, standard glycine betaine. Although glycine betaine was required for the proliferation of all examined Perkinsus species, supplementation of glycine betaine precursors, such as choline and betaine aldehyde, enhanced the proliferation of 4 Perkinsus species (P. marinus, P. chesapeaki, P. mediterraneus and P. honshuensis), but not of 2 others (P. olseni and P. beihaiensis). Thus, it was concluded that the ability to biosynthesise glycine betaine from its precursors varied among Perkinsus species, and that P. olseni and P. beihaiensis lack the ability required to biosynthesize glycine betaine for proliferation.

Mollusk and ostracode assemblages from the distal Old River Bed delta (ORBD) contribute to our understanding of the Lake Bonneville basin Pleistocene-Holocene transition (PHT) wetland and human presence on the ORBD (ca. 13,000–7500 cal yr BP). Located on U.S. Air Force-managed lands of the Great Salt Lake Desert (GSLD) in western Utah, USA, the area provided 30 samples from 12 localities. The biological assemblages and the potential water sources using 87Sr/86Sr analyses showed wetland expansion and contraction across the PHT, including the Younger-Dryas Chronozone (YDC). The record reflects cold, freshwater conditions, which is uncharacteristic of the Great Salt Lake Desert, after recession of Lake Bonneville. Lymnaea stagnalis jugularis, Cytherissa lacustris, and possibly Candona sp. cf. C. adunca, an endemic and extinct species only reported from Lake Bonneville, suggest cold-water environments. Between 13,000–12,400 cal yr BP, a shallow lake formed, referred to as the Old River Bed delta lake, fed by Lake Gunnison, as shown by 87Sr/86Sr ratios of 0.71024–0.71063 in mollusk fossils collected at the ORBD, characteristic of the Sevier basin. These findings add paleoenvironmental context to the long-term use of the ORBD by humans in constantly changing wetland habitats between 13,000–9500 cal yr BP.

Marine mollusk shells have been extensively used to provide radiocarbon (14C)-based chronologies in paleoenvironmental and archaeological studies, however uncertainties in age measurements are introduced because secondary factors such as vital effects and diet may influence 14C incorporation into these shells. Deep burrowing and deposit feeding mollusks, in particular, may incorporate “old” carbon resulting in apparently older ages than their contemporary environment. In this study, we present paired 14C and stable isotope (δ13C and δ18O) measurements for nine species of known-age bivalves having different feeding strategies and collected in six localities around the NE Atlantic. We exclude potential “old” carbon contamination in these known-age mollusk shells, acquire a better understanding of local ecology and provide an improved context for the environmental interpretation of 14C ages. Our results indicate that, in the NE Atlantic, marine mollusk-derived 14C ages provide a reliable basis for environmental and archaeological investigation, independently of vital effects and differences in microhabitats, feeding strategies and sample location—all of which are apparent from stable isotopes.

The Mockingbird Gap Clovis site is a surface archaeological site located along Chupadera Draw in central New Mexico. Coring of the draw during archaeological investigation of the Clovis assemblage on the adjacent uplands revealed evidence for a regionally rare continuous, stratified depositional record beginning immediately before the Younger Dryas chronozone (YDC). Thirty sediment samples from the draw adjacent to the archaeological site were analyzed for microinvertebrates (ostracodes and mollusks) and gyrogonites (calcareous algae) to reconstruct its environmental history. Wet-dry cycles marked the presence/absence of microfossils. Based upon microfossils, this investigation highlights environmental change marked by the evolution from wetter/cooler to warmer/drier conditions at the Mockingbird Gap site and its response to climate change and groundwater fluctuations during and after the YDC. Four biofacies are recognized: the pre-Ciénega setting is sterile. Holarctic species near the base of core 08-1 indicate cooling conditions prior to 13,000 cal yr BP during the early Ciénega phase. Warmer, more saline conditions characterize the late-Ciénega biofacies between 11,000 and 10,000 cal yr BP. Presence of gypsum during formation of the post-Ciénega phase and the most salinity tolerant species after 10,000 cal yr BP is consistent with the aridification typifying the early Holocene.

The extent of racemization of aspartic acid (Asp) has been used to estimate the ages of 9 shells of the epifaunal calcitic brachiopod Bouchardia rosea and 9 shells of the infaunal aragonitic bivalve Semele casali. Both taxa were collected concurrently from the same sites at depths of 10 m and 30 m off the coast of Brazil. Asp D/L values show an excellent correlation with radiocarbon age at both sites and for both taxa (r2Site 9 B. rosea  = 0.97, r2Site 1 B. rosea = 0.997, r2Site 9 S. casali = 0.9998, r2Site 1 S. casali = 0.93). The Asp ratios plotted against reservoir-corrected AMS radiocarbon ages over the time span of multiple millennia can thus be used to develop reliable and precise geochronologies not only for aragonitic mollusks (widely used for dating previously), but also for calcitic brachiopods. At each collection site, Bouchardia specimens display consistently higher D/L values than specimens of Semele. Thermal differences between sites are also notable and in agreement with theoretical expectations, as extents of racemization for both taxa are greater at the warmer, shallower site than at the cooler, deeper one. In late Holocene marine settings, concurrent time series of aragonitic and calcitic shells can be assembled using Asp racemization dating, and parallel multi-centennial to multi-millennial records can be developed simultaneously for multiple biomineral systems.

A thick alluvial sequence in central New Mexico contains the Scholle wet meadow deposit that traces upstream to a paleospring. The wet meadow sediments contain an abundant fauna of twenty-one species of freshwater and terrestrial mollusks and ten species of ostracodes. The mollusks and ostracodes are indicative of a local high alluvial water table with spring-supported perennial flow but without standing water. Pollen analysis documents shrub grassland vegetation with sedges, willow, and alder in a riparian community. Stable carbon isotopes from the wet meadow sediments have δ13C values ranging from − 22.8 to − 23.3‰, indicating that 80% of the organic carbon in the sediment is derived from C3 species. The wet meadow deposit is AMS dated 10,400 to 9700 14C yr BP, corresponding to 12,300 to 11,100 cal yr BP and overlapping in time with the Younger Dryas event (YD). The wet meadow became active about 500 yr after the beginning of the YD and persisted 400 yr after the YD ended. The Scholle wet meadow is the only record of perennial flow and high water table conditions in the Abo Arroyo drainage basin during the past 13 ka.

The distribution, sedimentology, and age of early Holocene Mangyshlakian deposits beneath the northern Caspian Sea are studied. Analyses and interpretations derive from high-resolution sub-bottom profiling, lithological analysis of core sections and boreholes, biostratigraphic analysis of mollusc shells, and radiocarbon dating. We show that the Mangyshlakian deposits overlie Upper Pleistocene Khvalynian deposits, filling valleys and depressions that formed during lower water levels, similar to present lacustrine water bodies (ilmeni) of the Volga Delta. Transgressive marine Holocene Neo-Caspian sediments that are filling facies overlie the Mangyshlakian deposits. The deposits are composed of both organogenous and terrigenous materials. The flora and fauna in the sediment indicate deposition into freshwater, differing from marine biota in the underlying and overlying sediments. Eighteen radiocarbon ages indicate that the Mangyshlakian deposits accumulated between 9 and 12 ka.

In northeastern Kuwait, ancient beach ridges and associated berms are separated from the present shoreline by a 4–6 km-wide sabkha. A diverse mollusk fauna in the beach ridges attests to a former open marine environment. A total of 21 AMS dates were obtained in this study. Thirteen mollusk samples from beach ridges yielded AMS dates ranging from ~ 6990 cal yr BP in the southeast to ~ 3370 cal yr BP in the northwest, suggesting a southeast to northwest age progression during the Holocene transgression. In contrast, four samples from berms throughout the study area yielded AMS dates of 5195–3350 cal yr BP showing no age progression; these berms consist largely of Conomurex persicus gastropods that aggregated by storms during a highstand at ~ 5000–3500 cal yr BP. The berms are presently at ~ + 6 m above sea level, 2–3 m above the beach ridges. Human settlements were common on the ridge crests before and after the highstand. Regression to present-day sea level commenced after the highstand, which is when the sabkha began forming. A landward, marine-built terrace, which yielded AMS dates > 43,500 14C yr BP, probably formed during Marine Oxygen Isotope Stage 5e and hence is not genetically related to the beach ridges.

One of the most intriguing episodes in the Quaternary evolution of the Grand Canyon of the Colorado River, Arizona, was the development of vast lakes that are thought to have backed up behind lava erupted into the gorge. Stratigraphic evidence for these deep lava-dammed lakes is expectedly sparse. Possible lacustrine deposits at six areas in the eastern canyon yielded no compelling evidence for sediment deposited in a deep lake. At two of the sites the sediment was associated with late Quaternary spring-fed pools and marshes. Water-lain silt and sand at lower Havasu Creek was deposited ∼3000 cal yr ago. The deposit contains an ostracode assemblage similar to that living in the modern travertine-dammed pools adjacent to the outcrop. The second deposit, at Lees Ferry, formed in a spring-fed marsh ∼43,000 cal yr ago, as determined by 14C and amino acid geochronology. It contains abundant ostracode and mollusk fossils, the richest assemblages reported from the Grand Canyon to date. Our interpretation of these sediments as spring-fed deposits, and their relative youth, provides an alternative to the conventional view that deposits like these were formed in deep lava-dammed lakes that filled the Grand Canyon.

A 25 m-thick outcrop section exposed at Torre Mucchia, on the sea-cliff north of Ortona, eastern central Italy, comprises a rare Middle Pleistocene succession of shallow-water and paralic sediments along the western Adriatic Sea. An integrated study of the section, including facies and microfacies analyses, and characterization of paleobiological associations (mollusks, fishes, ostracods, foraminifers and pollen), enable a detailed reconstruction of the paleoenvironmental and paleoclimatic conditions during deposition. The shallow-water deposits include a transgressive, deepening- and fining-upward shoreface to offshore-transition facies succession overlain by a regressive shoreface-foreshore sandstone body with an erosive base and a rooted and pedogenically altered horizon at the top that imply deposition during sea-level fall. This forced regressive unit is overlain by paralic strata forming a transgressive succession comprising palustrine carbonates and back-barrier lagoonal mudstones. The palustrine carbonates exhibit some of the typical features encountered in palustrine limestones deposited within seasonal freshwater wetlands (marl prairies). Following the sea-level rising trend, the freshwater marshes were abruptly replaced by a barrier-lagoon system that allowed deposition of the overlying mud-rich unit. Within these deposits, the faunal assemblages are consistent with a low-energy brackish environment characterized by a relatively high degree of confinement. The pollen record documents the development of open forest vegetation dominated by Pinus and accompanied by a number of mesophilous and thermophilous tree taxa, whose composition supports a tentative correlation with Marine Oxygen Isotope Stage 17. The new pollen record from Torre Mucchia improves our understanding of the vegetation development in the Italian Peninsula during the Middle Pleistocene and sheds new light on the role played by the most marked glacial periods in determining the history of tree taxa.

The main purpose of this study was to establish the pattern of variability of some hemato-immunological parameters in the mangrove oyster, Crassostrea rhizophorae, from 3 different environmental conditions in Santa Catarina Island: two natural habitats (mangrove and rocks of the coastal bay) and one oyster culture station. The water quality was adequate in all localities. The animals were collected seasonally over a period of one year (n = 30, per locality). The oyster hemolymph comprised two basic cell populations, hyaline (HH) and granular hemocytes (GH). Both cell populations contained carbohydrates and glycogen in their cytoplasm (periodic acid Schiff reaction, PAS staining) and the occurrence of lysosomes was suggested by the detection of acid phosphatase (Gomori's method). Both hemocyte populations were able of phagocytosis of zymosan particles in vitro and producing cytotoxic molecules, such as the superoxide anions (nitroblue-tetrazolium, NBT reduction). The oyster hemograms significantly differed in the different habitats and also among seasons. The total circulating hemocyte counts (THC) and the percentage of GH in the mangrove and rock oysters always differed from each other, but not from those of the cultured oysters. The highest THC and the lowest percentage of GH were always found in summer. The total plasma protein concentration (PC) exhibited a similar seasonal pattern in all oyster populations, with a marked decrease in spring. The PC of the cultured oysters was almost always significantly lower than that of the other oyster populations. The plasma agglutinating titer was higher in the cultured oysters regardless of season. The histological organization of the oyster digestive gland and gills was basically similar to that of other oyster species. Their structural aspect did not show any detectable alteration, corroborating that the oysters were in good health. The results of this study will serve as a basis for further analyses on the monitoring of C. rhizophorae health status and environmental quality in different aquatic habitats.

Gonadal neoplasms (germinomas) in softshell clams, Mya arenaria, have only been reported from locations in Maine, USA despite the fact that the geographic range of M. arenaria extends from Labrador to North Carolina on the east coast of North America. To more accurately determine the geographic distribution of this disease, adult clams (n = 18–60 per sample) obtained between 1989 and 1997 from sites along the entire coast of Maine and from Atlantic Canada (New Brunswick, Nova Scotia, and Prince Edward Island) were examined histologically for the presence of neoplasia. Gonadal neoplasms were present at 10 of the 28 locations sampled, including sites in Maine, New Brunswick, Nova Scotia, and Prince Edward Island, at prevalences ranging from 3.3 to 50% and at all stages of development. Prevalence and stage of development, however, were consistently greater at sites located between Penobscot Bay, Maine and Passamaquoddy Bay, New Brunswick. There was no correlation between mean clam size (shell length) and prevalence. Clams with neoplasia were predominantly female. To assess possible disease transmissibility and subsequent mortality rates, naïve clams were transplanted to a site where neoplasia is enzootic and placed in close proximity to clams having the disease. After 6 months, no evidence of neoplasia was found in the transplanted clams even though cumulative mortality (14.7%) was greater than that in local clams (3.4%). These results suggest that gonadal neoplasms in M. arenaria progress slowly and cause little mortality once present in an individual and may not have an infectious etiology. Loss of reproductive output is a potential long-term effect of the disease.