Effects of linuron on annual bluegrass control and Kentucky bluegrass cultivar tolerance were studied in field and growth chamber experiments. In controlled environment experiments, linuron at 0.06, 0.12, 0.25, 0.50, and 0.75 kg ai ha-1 was applied to pure stands of annual bluegrass and eight Kentucky bluegrass cultivars. Linuron at the two highest rates controlled annual bluegrass, reducing the clipping dry weight by more than 85% 4 wk after treatment, and by 65 to 92% 6 wk after treatment. Growth of Kentucky bluegrass was reduced with the most severe reduction occurring 2 wk after linuron application. All cultivars exhibited normal growth 8 wk after treatment. In field experiments, linuron at rates from 1.5 to 2.0 kg ai ha-1 controlled annual bluegrass in old (> 5 yr) Kentucky bluegrass stands, and in 16 cultivars of 1-yr and 2-yr-old Kentucky bluegrass stands, with little or no damage. At rates of 1.5, 2.0, and 2.5 kg ai ha-1 linuron, damage to newly seeded cultivars was moderate to severe. However, 6 to 7 wk after linuron application to newly seeded cultivars, stand density and turf quality were equivalent to untreated checks.

Sixty isolates of the plant pathogenic fungus Sclerotinia sclerotiorum (Lib.) de Bary and six isolates of Sclerotinia minor Jagger were evaluated in a controlled environment for virulence on leaves excised from 8-week-old dandelion plants. Significant negative correlations were obtained between the relative virulence of the isolates and the dry weights of dandelion plants inoculated in a controlled environment; and positive correlations were detected between the relative virulence of isolates and reduction in number of dandelion plants in turfgrass swards infested with inoculum of the isolates. In August 1988, an 80.7% reduction in the number of dandelion plants was detected in a turfgrass sward treated in 1987 with four applications of heat-killed seed of perennial ryegrass (100 g m−2 application–1) infested with isolate R30 of S. sclerotiorum, followed by six applications at the same rate in 1988. Populations of dandelions in untreated swards increased by 22.2% during the same period. Heat-killed seed of perennial ryegrass (100 g m−2) infested with isolate R30 applied simultaneously with dandelion seed (25 g m−2) onto a sward of Kentucky bluegrass reduced the establishment of dandelion seedlings by 85.5%. Necrosis or discoloration did not develop on Kentucky bluegrass, creeping bentgrass, annual bluegrass, or quackgrass treated with inoculum of Sclerotinia. Sclerotia of Sclerotinia spp. were found, on occasion, in crowns but not on the foliage of diseased dandelions.

Under greenhouse conditions, annual bluegrass (Poa annua L.), common groundsel (Senecio vulgaris L.), shepherd's purse [Capsella bursa-pastoris (L.) Medicus], small nettle (Urtica urens L.), canola (Brassica napus L. ssp. napus), and pea (Pisum sativa L.) differed in sensitivity to ultraviolet (UV) radiation. Of the weed species, annual bluegrass was the least sensitive; whereas, among the crop species, canola was about sevenfold more sensitive than was pea. The sensitivity of a species to UV radiation was highly dependent upon its stage of development. The study indicates some potential for using UV radiation to control weeds, but the method needs further investigation to unravel the selectivity of the methods and potential health hazards.

Field studies were conducted for 4 yr to investigate the effects of tillage and herbicide programs on weed control and wheat (Triticum aestivum L. ‘Potomac’ in 1981 and ‘Wheeler’ from 1982 to 1984) grain yields in a wheat-soy bean [Glycine max (L.) Merr.] double-crop rotation. Predominant weed species were common chickweed [Stellaria media (L.) Vill. # STEME], corn chamomile (Anthemis arvensis L. # ANTAR), and annual bluegrass (Poa annua L. # POAAN) at the onset of research and cheat (Bromus secalinus L. # BROSE) and soft brome (Bromus mollis L. # BROMO) at the conclusion of the study. Control of all species was excellent with conventional tillage and no-till plus nonselective herbicides but was significantly less with no-till without nonselective herbicides and with minimum tillage. After 4 yr, maximum wheat grain yields were significantly higher in conventional- than in minimum- or no-till systems.

Clear and black polyethylene mulches were placed over raised seed beds in four studies during a 3-yr period. At intervals during treatment times of up to 12 weeks, soil samples were taken and the recovered seed were tested for germination. The number of seed killed and depths to which they were killed varied with species. The study included natural seed populations of annual bluegrass (Poa annua L. ♯3 POAAN), barnyardgrass (Echinochloa crus-galli (L.) Beauv. ♯ ECHCG), annual species of Cyperus, dayflower (Commelina communis L. ♯ COMCO), and goosegrass (Eleusine indica L. Gaertn. ♯ ELEIN). After 40 days exposure under clear mulch, seed of dayflower were killed to a depth of 11 cm. Under the same conditions, seed of annual Cyperus sedges and barnyardgrass were killed only in the upper 3 to 4 cm. After 3 weeks under clear plastic, germinable seed of goosegrass were recovered only at depths greater than 5 cm. Seed in plots under clear polyethylene mulch were killed more quickly than those in plots covered with black polyethylene mulch.

Field experiments were conducted in 1982 through 1986 to evaluate the effect of six postemergence grass herbicides, clethodim, the methyl ester of diclofop, the butyl ester of fluazifop-P, the methyl ester of haloxyfop, the methyl ester of quizalofop, and sethoxydim, on 31 seedling grass species. All herbicides except diclofop controlled most of the species tested. None of the herbicides controlled rattail fescue or red fescue. Only clethodim and haloxyfop controlled annual bluegrass.

Two previously identified triazine-resistant annual bluegrass (Poa annua) biotypes in Mississippi were evaluated to determine the level as well as the mode of resistance compared to a triazine-susceptible biotype. Annual bluegrass seeds were collected at two sites (Meridian and Tupelo, MS) from field plots previously treated with 11.2 kg ai/ha simazine. Seeds were planted on agar containing simazine (0, 0.001, 0.01, 0.1, 1, 10, or 100 mM) or diuron (0, 0.01, 0.1, 1, 10, 100, or 1,000 μM), placed in the growth chamber with an 8 h daylength and alternating temperatures of 15 and 20 C, and allowed to grow for 3 wk, after which fresh weight was determined. Data were subjected to regression analyses and the amount of simazine or diuron required to reduce fresh weight to 50% of the untreated plants was determined. These data indicated a greater than 1,000-fold level of resistance to simazine with either biotype. Diuron concentrations of 5.0, 3.0, and 3.2 μM were required to reduce fresh weight to 50% of the untreated for the triazine-susceptible, Meridian, and Tupelo biotypes, respectively. Sequencing a portion of the chloroplast psbA gene revealed a serine 264 to glycine mutation, which reduced the affinity of the triazine herbicides for the QB-binding niche on the D1 protein in photosystem II.

Morphological and physiological variation among annual bluegrass populations from the green, fairway, and rough were measured to determine the role annual bluegrass infestations outside the green play in maintaining the population in the green. Annual bluegrass populations from the fairway and the rough were similar to each other but differed from that of the green in habit, dry mass production, flowering, seed size, and germination. Seeds from each population established best in the type of turf from which they originated. It is concluded that, since few genotypes were common to the green and its surroundings, the populations in the fairway and rough played little or no role in maintaining the population of the green. The same is likely to be true wherever the management of greens and their surroundings differs sufficiently for marked population differentiation to occur. This finding does not preclude the possibility that some genotypes adapted to the green persist in the surroundings, and that these genotypes serve as sources of seeds for the colonization or reinfestation of annual bluegrass-free greens.

Postemergence applications of BAS 9052 OH, {2-[1-(ethoxyimino)butyl]-5-[2-(ethylthio)propyl]-3-hydroxy-2-cyclohexen-1-one}, at 0.25 and 0.5 kg ai/ha were made to 27 temperate and 28 tropical species of Gramineae. Annual bluegrass (Poa annua L. ♯3 POAAN) and rattail fescue (Festuca myuros L. ♯ VLPMY) were resistant to these rates of BAS 9052 OH. Five species of fescue and three species of bluegrass received postemergence applications of BAS 9052 OH at rates of 0.1 to 6.4 kg ai/ha. The germination of seeds and the subsequent growth of the seedlings of these species as influenced by various concentrations of BAS 9052 OH were also studied. Meadow fescue (Festuca pratensis Huds. ♯ FESPR), tall fescue (Festuca arundinacea Schreb. ♯ FESAR), Kentucky bluegrass (Poa pratensis L. ♯ POAPR), and rough-stalked meadowgrass (Poa trivialis L. ♯ POATR) were most susceptible; annual bluegrass was somewhat less resistant; hard fescue (Festuca longifolia Thuill) was resistant; red fescue (Festuca rubra L. ♯ FESRU) and rattail fescue were very resistant.

California and Michigan strains of Xanthomonas campestris pv. poannua were compared as biocontrol agents for the annual and perennial subspecies of annual bluegrass. Additionally, inoculation frequency was evaluated for improved control of the perennial subspecies in New York. The two bacterial strains were similarly virulent to annual bluegrass in both growth chamber and field tests. When inoculated once per week for 4 wk, the annual and perennial subspecies were controlled 92 and 82% in growth chamber tests, but only 11 and 7% in field tests, respectively. Control of established annual bluegrass in the field was improved to a maximum of about 40% by increasing inoculation frequency to three times per wk for 4 wk, but recovery occurred 2 to 5 wk after inoculations were discontinued. Creeping bentgrass was not affected by the bacterium.

Sethoxydim {2-[1-(ethoxyimino)butyl]-5-[2-(ethylthio)propyl]-3-hydroxy-2-cyclohexen-1-one} is used to control weedy grasses in red fescue (Festuca rubra L. # FESRU and F. rubra ssp. commutata), an important seed crop in Oregon. The sethoxydim rate required to reduce growth rate of 11 cultivars of red fescue and four cultivars of bentgrass (Agrostis tenuis Sibth. # AGSTE and A. stolonifera L. var. palustris # AGSST), a common contaminant in red fescue fields, by 50% (GR50) was determined in greenhouse and laboratory experiments. Field experiments compared only red fescue cv. ‘Pennlawn’ and bentgrass cv. ‘Penncross'. The GR50 values for Pennlawn red fescue in the field, the greenhouse, and the laboratory were 30 kg ai/ha, 15 kg/ha, and 5300 μM, respectively. Penncross bentgrass was approximately 400, 1400, and 12 900 times more susceptible than Pennlawn red fescue in the field, in the greenhouse, and in laboratory experiments, respectively. Plants were at different growth stages in the different experiments. The xylene and surfactant used as a solvent for the technical grade sethoxydim was as toxic to red fescue without sethoxydim as when sethoxydim was included. Cultivars of red fescue differed in their response to sethoxydim, but cultivars of bentgrass did not. The GR50's of other species tested in the greenhouse were >4 kg/ha for annual bluegrass (Poa annua L. # POAAN), 0.22 kg/ha for tall fescue (F. arundinacea Schreb. # FESAR), 0.37 kg/ha for downy brome (Bromus tectorum L. # BROTE), and 0.017 kg/ha for Italian ryegrass (Lolium multiflorum Lam. # LOLMU).

Nonfungicidal effects of agricultural fungicides on crop plants have been reported previously; however, there are few reports of nontarget effects of fungicides on weedy species. Field research trials in Oregon demonstrated that the growth of several broadleaf weeds was reduced after multiple applications of the fungicide propiconazole. Greenhouse experiments confirmed that preemergence applications of propiconazole reduced the biomass accumulation of several common broadleaf and grass weeds 15 to 63%. Laboratory experiments were performed on redroot pigweed, the most sensitive species, to examine the effects of propiconazole on germination and early seedling growth. Redroot pigweed germination and total seedling length (root plus shoot) were reduced at propiconazole concentrations above 37 and 0.36 mg/L, respectively. Growth-regulating effects of fungicides such as propiconazole on the germination and early growth of weeds may contribute to integrated weed management, especially when adequate moisture ensures the presence of germinating seeds and small seedlings throughout the growing season.