Triazine-resistant (R) biotypes of common lambsquarters, yellow foxtail, and hairy fleabane were found in the Province of Córdoba (Andalusia, Southern Spain). The former two R biotypes came from atrazine-treated cornfields, whereas the latter came from simazine-treated nontilled olive orchards. The R biotypes of common lambsquarters and yellow foxtail and the R biotype of hairy fleabane survived at doses up to 5 kg ai/ha of soil-applied atrazine or simazine, respectively. Photosynthetic electron transport in R biotypes was unaffected by atrazine and simazine but was inhibited by diuron, as shown by fluorescence induction measurements in whole leaves. In Hill reaction assays, the R biotypes showed high resistance to atrazine and simazine (resistance factors in the range of 350 to 550), medium to high resistance to ametryn, terbumeton, metribuzin, and monolinuron (resistance factors in the range of 80 to 250), slight resistance to diuron and methabenzthiazuron (resistance factors in the range of 1.1 to 15.7), and reverse resistance to swep, ioxynil, and DNOC (resistance factors less than 1). It is concluded that the R biotypes have a chloroplast mode of resistance similar to that previously described for other triazine-resistant weed biotypes.

Cross-resistance of the paraquat-resistant (R) hairy fleabane to other compounds that accept electrons from photosystem I (PSI) or produce toxic oxygen species was determined by chlorophyll loss, electron microscopy, and chlorophyll fluorescence suppression. Although the R bioype is approximately 100 x more resistant to paraquat than the susceptible (S) biotype based upon the assays for tissue damage, little or no cross-resistance was observed to a number of other PSI electron acceptors, including the bipyridilium herbicide morfamquat. A low level of resistance (approximately 10-fold) was noted to diquat and the singlet oxygen generator rose bengal. As measured by chlorophyll fluorescence suppression, the R biotype was about 100-fold resistant to paraquat, but only 10-fold resistant to diquat, and exhibited no resistance to morfamquat. Because differences observed with this protocol are direct measures of the ability of the herbicide to reach the active site and the results correlate with the level of resistance observed by chlorophyll bleaching or electron microscopy, these data suggest that compartmentalization is the major factor in paraquat resistance in hairy fleabane.

Applications of isopropylamine glyphosate at 0.28, 0.56, 0.84, and 1.12 kg ae/ha in combination with the dimethylamine salts of 2,4-D or dicamba at 0.14, 0.28, 0.42, and 0.56 kg ae/ha produced additive or synergistic field bindweed control compared to the herbicides applied alone. Leaf and root growth was inhibited more from herbicide combinations than would be predicted from the effects of the chemicals applied alone at the same rate. The uptake of 14C from glyphosate into the treated leaf and its accumulation in roots increased when 2,4-D or dicamba was combined with the 0.28 kg/ha rate of 14C-glyphosate. The combination of 2,4-D or dicamba with a higher (0.84 kg/ha) 14C-glyphosate rate did not change total absorption of 14C from glyphosate. However, compared to 0.84 kg/ha of 14C-glyphosate applied alone, less 14C accumulated above the treated leaf and more accumulated in the roots when 2,4-D was added to the glyphosate. The combination of glyphosate with 2,4-D or dicamba generally resulted in both increased uptake of 14C from 2,4-D or dicamba and greater accumulation in the roots. The additive or synergistic field bindweed control observed from mixtures of glyphosate with 2,4-D or dicamba appeared to be due to greater accumulation of the herbicides in the roots.

Foliar sprays of the monoethanolamine salt, potassium salt, free acid, and 1-decyl ester of clopyralid were more effective in killing greenhouse-grown honey mesquite than the 2-ethylhexyl ester at rates of 0.28 ae/ha or less. More clopyralid was transported to the lower canopy from application of the monoethanolamine salt and potassium salt than the 2-ethylhexyl ester of clopyralid at 4 h or 1, 3, or 8 days after treatment. Application of the monoethanolamine salt and the 2-ethylhexyl ester to leaves with a pipet indicated that about twice as much clopyralid was absorbed within 15 min from the ester form (26%) than from the amine form (12%) of the total recovered. However, after 24 h, absorption of the ester was less than the amine. More than twice as much clopyralid was transported from the treated leaf after application of amine than the ester. Only the acid form of clopyralid was transported away from the site of application of either ester or amine.

Seedlings of kudzu were grown at 350, 675, or 1000 μl/L CO2 in controlled-environment chambers. At elevated CO2 concentrations, maximum leaf expansion rates were approximately 40% greater, leaves were fully expanded several days sooner, fully expanded leaves were larger at each leaf position, and leaf production rates were increased 12%. Peak starch accumulation was much greater in plants grown at elevated CO2 concentrations. Total xylem water potentials were higher (less negative) at full hydration, and osmotic potentials were decreased (more negative) by CO2 enrichment. At 1000 μl/L CO2, leaf turgor pressure was twice that at 350 μl/L CO2. Results suggest that leaf expansion rates and leaf expansivity may have been increased due to higher turgor pressure at the higher CO2 concentrations. The potential for successful seedling establishment may be enhanced as the atmospheric CO2 concentration continues to rise, increasing kudzu invasiveness.

Absorption of ethyl ester of chlorimuron by excised velvetleaf root tissue was investigated. Chlorimuron uptake increased rapidly, reaching a maximum after 2 h. After 4 h, however, a portion of the previously absorbed herbicide was lost to the external solution. The temperature coefficient (Q10) for chlorimuron absorption between 15 and 25 C was 1.9. The herbicide uptake was severely inhibited by metabolic inhibitors, DNP and KCN, and by anaerobic conditions (anoxia). Results of permeation experiments indicated that chlorimuron did not accumulate in the root tissue against a concentration gradient, and Ci/Co reached 1.0 after a 2-h permeation period. Chlorimuron efflux was continuous and after 2 h of washing the tissue in a herbicide-free solution, 90% of the previously absorbed herbicide was removed, suggesting that its accumulation in the tissue was reversible.

Antidotes were evaluated for their ability to prevent corn and sorghum injury caused by imazaquin. Plant injury was reduced in both preemergence and early postemergence imazaquin applications. Naphthalic anhydride (NA) seed treatment was the most consistent compound in reducing the imazaquin injury. Seed treatment with CGA 92194 or flurazole also gave injury protection to corn and sorghum, while soil treatment with dichlormid provided the least protection from imazaquin injury. Treatment with the antidotes did not affect the acetolactate synthase (ALS) (EC4.1.3.18) activity in corn or sorghum tissues. Imazaquin treatments decreased extractable ALS activity but this decrease was eliminated, partially or totally, by the most effective antidotes. Antidote treatments had little effect on absorption and distribution of 14C in plants growing in soil treated with 14C-imazaquin. Antidote treatments increased the metabolism of 14C-imazaquin to both soluble and unextractable 14C after 24 h of exposure. The increased rate of imazaquin conversion to less toxic metabolites when antidotes were used resulted in a reduction in imazaquin injury to corn and sorghum.

Sulfometuron, when applied as a foliar and/or soil application, prevented regrowth of bahiagrass. Sulfometuron application did not reduce regrowth of centipedegrass regardless of method of application. Sulfometuron was absorbed by the roots and foliage of centipedegrass and bahiagrass. Symplasmic translocation of the herbicide was evident in both species. Translocation of foliar-applied sulfometuron increased from approximately 1% at 48 h after application to 23% at 72 h in bahiagrass. Metabolism of sulfometuron was greater in centipedegrass (69% of foliar-applied, 10% of root-applied) at 72 h after application than in bahiagrass (30% of foliar-applied and 4% of root-applied). Tolerance of centipedegrass to sulfometuron appeared to be related to a high degree of herbicide metabolism in this species.

Establishment of seedlings of big sagebrush subsequent to range weed control treatments limits the economic feasibility of such treatments for increased forage production. This study investigated the dynamics of seed production by this species as one step in understanding seedling establishment. Studies were conducted on five different ecological sites along the arid extremity of big sagebrush distribution in western Nevada. Demography, physical dimensional parameters, biomass, and community structure were determined for each site and related to seed production over a 4-yr period. In addition, seeds harvested from each site were used to grow plants that were transplanted to establish reciprocal gardens at all sites. Seeds were subsequently harvested from the plants in these gardens. The average stand studied was capable of producing about 50 million seeds/ha annually. The plant characteristics most closely correlated with seed production were leaf weight, canopy density, and root crown diameter. The stand that consistently had the highest average seed production had the lowest woody biomass accumulation rate. In reciprocal gardens, plants grown from this stand had the highest average seed production. Seed production of big sagebrush plants appears to be related to site characteristics, shrub biomass characteristics, and inherent genetic potential of the plants in a given collection.

Morphological and physiological variation among annual bluegrass populations from the green, fairway, and rough were measured to determine the role annual bluegrass infestations outside the green play in maintaining the population in the green. Annual bluegrass populations from the fairway and the rough were similar to each other but differed from that of the green in habit, dry mass production, flowering, seed size, and germination. Seeds from each population established best in the type of turf from which they originated. It is concluded that, since few genotypes were common to the green and its surroundings, the populations in the fairway and rough played little or no role in maintaining the population of the green. The same is likely to be true wherever the management of greens and their surroundings differs sufficiently for marked population differentiation to occur. This finding does not preclude the possibility that some genotypes adapted to the green persist in the surroundings, and that these genotypes serve as sources of seeds for the colonization or reinfestation of annual bluegrass-free greens.

Paraquat and diquat were used from 1963 until 1986 to control capeweed and other annual weeds on an alfalfa field at Elmhurst, Victoria, Australia. From 1983, control of capeweed with these bipyridyl herbicides was no longer satisfactory. In experiments conducted on this field in 1986 and 1987, 800 g ai/ha of diquat was required to equal the control of capeweed obtained by 100 g/ha diquat in an adjacent pasture field with no history of herbicide use. There were substantial numbers of a diquat-resistant biotype within the population in the alfalfa field. It is concluded that the diquat-resistant biotype of capeweed became dominant due to the strong selection pressure provided by over 20 yr of use of the bipyridyl herbicides.

Longspine sandbur germinated readily after 3 months of storage at 0 to 4 C. Germination was 15 to 82 and 10 to 76% for seed retained by or that passed through a 1.7-mm-mesh screen, respectively, and was greatest in complete darkness near 30 C. Light inhibited seed germination at most of the temperatures tested. Incubating imbibed seed at 40 C and 40/10 C for 3 weeks reduced the viability of seed that did not germinate by more than 50%. Primary seed within the bur germinated 77% and secondary seed germinated 35% during 2 weeks at 35/10 C (16 light/8 dark h). Primary seed placed in polyethylene glycol (PEG) solutions ranging from −0.5 to −1.5 kPa water potential germinated as much or greater than seed placed in water, while secondary seed germination was inhibited by similar osmotic potentials. Seedlings emerged from depths to 11 cm but emerged most readily from 1- to 3-cm depths.

The influence of depth (0 to 30 cm) and duration (1 to 24 months) of burial on the deterioration, germination, and viability of ripgut brome seed was studied in the field. Both surface-sown and buried ripgut brome seed were depleted within 15 months. Persistence of surface-sown seed declined relatively slowly during the first year, falling from 83 to 62 to 23% after 1, 9, and 12 months, respectively. Seed covered by soil, however, germinated more rapidly, with less than 10% of the initial population ungerminated after 1 month at all depths. The mode of seed disappearance was closely related to whether or not the seed was covered with soil. Seed loss at depths of 1 to 30 cm was primarily due to germination in situ, with little effect from viability loss or enforced or induced dormancy. In contrast, the persistence of surface-sown seed was due primarily to induced dormancy for up to 12 months, with viability loss and enforced dormancy becoming important thereafter. Tillage practices aimed at providing favorable germination conditions may reduce ripgut brome seed survival in the soil. Because seed is relatively short lived, seed supply in soil may be reduced by short-term rotation to a crop that allows for effective control of ripgut brome.

Experiments conducted for two consecutive years indicated that barnyardgrass, large crabgrass, and giant foxtail interfered with the growth and development of container-grown ‘Gold Drop’ bush cinquefoil. Grass weed densities ranging from one to six weeds per container reduced Gold Drop bush cinquefoil growth. Bush cinquefoil shoot dry weight, averaged over all grass weeds, was decreased as much as 75% after 83 days of interference. The reduction of cinquefoil growth was independent of the number of grass weeds in the container.

Field studies were conducted in 1985 and 1986 to evaluate the stability of reciprocal interference relationships between common cocklebur and soybean under high and low soil moisture conditions. A significant soil moisture differential was established with portable rain exclusion shelters. Well-watered and drought-stressed common cocklebur reduced soybean yield 29 and 12%, respectively. Drought-stressed common cocklebur interfered with soybean over a shorter distance and the magnitude of the effect at a given distance was reduced. The reduced common cocklebur interference in drier soils was attributed to both common cocklebur and soybean growth responses to moisture stress. First, moisture stress caused greater reductions in common cocklebur canopy diameter, stem diameter, node number, and plant height than in soybean. Second, the soybean yield potential was reduced by moisture stress. The reduction in yield potential decreased the effect of the weed interference. Third, soybean canopy development was slowed, and canopy closure that occurred in about 12 weeks in well-watered soybeans never occurred in the moisture-stressed soybeans. This reduced the degree of light interference between both the common cocklebur and soybean and among the soybean plants. The results of this study indicate that the reciprocal interference relationships between common cocklebur and soybean are not stable across soil moisture conditions. The implications of unstable competitive parameters must be considered as threshold models are developed for various field crops.

The effects of soybean row width and redroot pigweed density on growth of crop and weed were studied in field trials in 1983, 1984, and Structural relationships within the canopies of soybean and redroot pigweed in relation to row width and weed density were studied in 1984 and 1985 to assess canopy geometry in relation to intra- and interspecific competition. Early in the growing season, soybean biomass was reduced in the presence of both high and low densities of pigweed. Pigweed biomass was also reduced in the presence of soybeans, especially when grown in narrow rows (25 cm). By midseason, pigweed's contribution to total biomass had reached 43% in wide-row (76-cm) stands and 24% in narrow rows. Soybean produced two to four times more leaf area than pigweed during the first half of the growth season. Narrow-row planting favored soybean leaf area production. Soybean LAI values from weedy stands were reduced compared to those from weed-free stands. Pigweed's contribution to total leaf area averaged 29% in wide-row spacing and 15% in narrow rows. Pigweed leaf area was concentrated in the upper strata of the canopy and thus reduced light available to soybean leaves lower in the canopy. Leaf area distribution patterns suggested that soybean and pigweed were competing for light even though soybean had produced more leaf area than pigweed. These relations were consistent from year to year in spite of variable water conditions.

Substances having anesthetic-like properties were examined for stimulation of redroot pigweed and witchgrass seed germination. Chemicals included the n-alcohols through C-5, 2-propanol, benzyl alcohol, diethyl ether, chloroform, and 2-bromo-2-chloro-1,1,1-trifluoroethanol. Redroot pigweed seeds required the far-red absorbing form of phytochrome (Pfr) for an anesthetic substance to increase germination, but in witchgrass seeds the active anesthetic substances stimulated germination without added Pfr. The correlation coefficient comparing relative activity (RA) versus the membrane/buffer partition coefficient (M/B) of the active substances was very poor (−0.37) for pigweed seeds but was −0.91 for witchgrass. Leakage of cellular materials, including electrolytes, substances absorbing at 280 nm, and amino acids from seeds treated with either active or inactive anesthetics, was not particularly indicative of effects on germination in both species. Application of increased pressure (0.965 MPa) during anesthetic treatment, which in animals prevents anesthesia, was found to act oppositely and increase seed response to ethanol in redroot pigweed seeds, but in witchgrass seeds pressure suppressed the stimulatory action of ethanol. In both species, pressure appeared to have an action of its own, but for this action to be expressed the presence of a stimulatory anesthetic was also required. The results do not appear to support a good relationship of anesthetic activity with seed membrane lipophilic components in redroot pigweed seeds, but the relationship seems likely for witchgrass seeds.

Effects of difenzoquat on wild oats grown under controlled environmental conditions were studied. Seedling height and fresh weight were significantly reduced 5 days after postemergence treatment. Dose-dependent increase in cell membrane permeability was detected after a 12-h exposure to the herbicide. Scanning electron micrographs showed normal leaf hairs and cuticular wax but swollen guard cells 10 days after treatment. Ultrastructural changes occurred before the visible symptoms. The primary effect of difenzoquat appears to be the disruption of the tonoplast and plasmalemma. The tonoplast showed greater damage than the plasmalemma. Secondary effects included damage to mitochondria and chloroplasts. Mitochondria were swollen and often ruptured, but the effect did not increase with the duration of exposure. Chloroplasts became spherical in shape, and their contents were also affected. The changes included: accumulation and then disappearance of starch granules, swelling of frets, fusion of granal thylakoids, detachment and then rupture of the outer membrane of the envelope, and clumping of ribosomes. By contrast, natural senescence caused greater injury of the plasmalemma than the tonoplast, a marked increase in size of plastoglobuli, and loss of starch grains without early accumulation.

Crown bud, tiller, and rhizome development was studied in 10 quackgrass biotypes at two stages (three-to four- and seven- to eight-leaf stages) of development to determine if differential development of these structures influenced control with sethoxydim and haloxyfop. There was a differential response among the 10 biotypes to both sethoxydim and haloxyfop at both stages of development. The biotypes differed in the number of crown buds, tillers, and rhizomes that formed at both stages of development. In the three- to four-leaf stage, the biotypes with the greatest number of crown buds were the most difficult to control. These results suggest that in the susceptible stage (three-to four-leaf) the quackgrass biotypes with the least number of crown buds will be the easiest to control; however, by the time all the biotypes reach the seven- to eight-leaf stage even the biotypes with the lowest number of crown buds have enough crown buds that they become difficult to control with either sethoxydim or haloxyfop.

Field experiments were conducted at two locations in Georgia to evaluate wild radish control and soft red winter wheat tolerance of herbicides applied February 1 (one- to five-tiller stage) or March 1 (three- to seven-tiller stage). Bromoxynil controlled wild radish with no wheat grain or forage yield reductions in any experiment. Thiameturon controlled wild radish when applied at rates >0.02 kg/ha on March 1. Metribuzin, dimethylamine salt of 2,4-D, and dimethylamine salt of MCPA provided late-season control of wild radish. February 1 treatments of metribuzin reduced wheat stands at Plains. The difference was attributed to environmental conditions, wheat tiller number at application, and possibly to differences in soil fertility at planting. Metribuzin, thiameturon, dimethylamine salt of dicamba, MCPA, and 2,4-D reduced wheat forage yield at Tifton. Dicamba did not control wild radish and reduced grain yield when applied at a rate of 0.3 kg ai/ha on March 1.