The patterns and extent of genetic variation among 56 cashew germplasm with respect to 32 qualitative and 33 quantitative traits were evaluated for two successive years in the present study. Additionally, maturase K gene-based genetic diversity among those breeding materials was also assessed. The cashew hybrids were developed from five crosses (local parent × 2/9 Dicherla; H-2/15 × red hazari; WBDC-V × JGM-1; BLA-39-4 × H-2/15 and H-2/15 × yellow hazari) involving eight parents of Indian cashew. Different genotype groups (parents and their hybrids) showed significant variation in both the years of assessment based on quantitative characters. The highest Shannon–Weaver diversity (H′) was obtained for the colour of the young leaf (0.96), possibly indicating differential exposure to sunlight, mixing of various pigments and another set of chemicals such as phenolics, carotenoids, etc. in trees. From correlation studies, canopy spread, tree spread, nuts/m2 and nuts/panicle were found to be significantly and positively correlated with nut yield. In the year 2021, nuts/m2, area and tree height were the significant explanatory variables that explained 80% of the variation in the yield, whereas in 2022, nuts/m2, tree area, nuts/panicle, kernel weight, shell thickness, inflorescence breadth and sex ratio explained 86% of the variation. Principal component analysis indicated that the genotypes under study are diverse enough to be exploited for the future cashew improvement programmes.

The A allele of the 5-HT2A gene (–1438A/G polymorphism) has been associated with anorexia nervosa in four studies, but not in three others. One possibility to explain such a discrepancy is that the A allele acts as a modifying rather than a vulnerability allele. To test this hypothesis, we increased our initial sample of 102 trios [Mol. Psychiatry 7 (2002) 90] with 43 new patients with anorexia nervosa and 98 healthy controls. In addition to confirming the absence of association on the global sample of 145 patients, we found that patients with the A allele had a significantly later age at onset of the disease (P = 0.032). Furthermore, the A allele was also transmitted with an older age at onset (P = 0.023) using a quantitative-trait TDT approach. The A allele may thus act as a modifying factor (delaying onset), potentially explaining variations of allele frequency across samples, in which differences in average age at onset are not only possible, but also expected. Taking into account vulnerability genes, but also genes modifying the expression of the disorder, will help to disentangle the complexity of the etiological factors involved in anorexia nervosa.

Yellow nutsedge is one of the most problematic weedy sedges in rice–soybean systems of the Mississippi Delta region. An acetolactate synthase (ALS)-inhibiting, herbicide-resistant (Res) yellow nutsedge biotype was recently documented in eastern Arkansas, which showed intermediary growth habit between yellow nutsedge and purple nutsedge and also exhibited differential photoperiodic sensitivity to flowering. The objectives of this study were to: (a) determine variation in reproductive characteristics of the Res biotype and three susceptible (Sus) yellow nutsedge biotypes, (b) understand the influence of photoperiod on growth and reproduction, (c) understand the potential role of seeds in population establishment, and (d) elucidate the phylogenetic relationships between the Res yellow nutsedge biotype and purple nutsedge. Tuber production per plant and tuber weight of the Res biotype were less than that of the Sus biotypes. Differences in quantitative traits, such as shoot and tuber production existed between the Res and Sus biotypes for photoperiods ranging from 12 to 16 h. Generally, photoperiods greater than 12 h increased shoot development in all yellow nutsedge biotypes, with differential responses among the biotypes. Number of tubers reached the maximum for the Res biotype at a 14-h photoperiod. Over a 90-d period, inflorescence formation was only observed in the Res biotype with maximum flowering and seed production in the 14-h photoperiod. Subsequent tests revealed up to 18% seed germination, suggesting that seed could also play a role (in addition to tubers) in the persistence and spread of the Res yellow nutsedge. Phylogenetic analysis based on ribosomal DNA internal transcribed spacer (ITS) regions and mitochondrial nad4 gene intergenic spacer sequences indicated that the Res biotype was more closely associated with Sus yellow nutsedge biotypes. Nevertheless, 100% similarity for the nad4 gene sequences between the Res yellow nutsedge biotype and a reference purple nutsedge suggests that the Res biotype is likely a result of hybridization between yellow and purple nutsedges, which perhaps explains the intermediary growth characteristics observed in the Res biotype.

The study examines phenotypic characterization of Central Highland and Woyto-Guji goat breeds at Meta Robi and Konso districts of Ethiopia. Purposive and random sampling techniques were employed to select sample villages and respondent. For administration of semi-structured questionnaire and phenotypic characterization, a total of 240 households and 601 adult animals, respectively, were selected. The average goat flock size (31.25 ± 22.46) owned per household of Konso was significantly (P < 0.01) higher than Meta Robi (12.73 ± 8). In Konso, natural pasture was the most frequently mentioned feed source during wet season, while hay was the most important feed source during dry season. On the other hand, in Meta Robi, natural pasture was the most frequently mentioned feed source during wet and dry seasons. Coat colour type, horn shape, ear orientation, wattle and beard were found to differ highly significantly (P < 0.001) among the two goat breeds. Goat breed had a significant effect (P < 0.001) on body weight and other body measurements except pelvic width (P > 0.05). The least-square means of body weight, body length, height at wither, chest girth, chest width, ramp length, horn length, ear length and pelvic width of Central Highland female goats were 29.5 ± 0.2 kg, 62.2 ± 0.2 cm, 67.5 ± 0.2 cm, 72.9 ± 0.2 cm, 13.4 ± 0.1 cm, 19.7 ± 0.1 cm, 12.8 ± 0.2 cm, 14.6 ± 0.1 cm and 13.5 ± 0.1 cm, respectively. The corresponding values for Woyto-Guji female goats were 24.8 ± 0.3 kg, 57.4 ± 0.2 cm, 61.9 ± 0.2 cm, 68.3 ± 0.2 cm, 12.1 ± 0.1 cm, 17.3 ± 0.1 cm, 10 ± 0.2 cm, 13 ± 0.1 cm and 13.4 ± 0.1 cm, respectively. The observed variations in production system and morphological traits among the sample populations coupled with their adaptive traits would indeed justify the need for designing breed improvement programme for both breeds.

The study was carried out in selected districts in the Northwestern Amhara, from October 2012 to May 2013. The objective of the study were to undertake on-farm and on-station phenotypic characterization of Fogera Cattle in comparison with two different local cattle population, to characterize the population structure and to identifying trait preferences, breeding management and to recommend breeding strategy for Fogera cattle. Both purposive and random samplings were employed. Data were gathered through semi-structured questionnaire, focus group discussions, field observations, census data, direct count and body measurements. About 126 smallholder farmers were interviewed. About 21 quantitative and 17 qualitative phenotypic data types were also generated from 332 cattle. The Effective population size (Ne) and rate of inbreeding (ΔF) were calculated from the counted population structure data. Both GLM procedures of SAS and descriptive statistics of SPSS software's were employed for data analyses. The results indicated that Fogera cattle were kept mostly for milk (97.62 percent). The main threats identified for the survival of Fogera cattle were scarcity of feed resources and interbreeding with other indigenous cattle, which are less demanding in terms of feed. Fogera cattle population has specific morphological appearance. Generally about 65.2 percent of male pure-Fogera cattle population are having large hump and large dewlap (93.5 percent) with cervico-thoracic (82.6 percent) hump position and long tail (97.8 percent), respectively. The coat pattern of male pure-Fogera cattle is dominated by the spotted coat pattern (82.6 percent) with 43.5 percent white black and 39.1 percent black white coat colour. Female Fogera cattle have medium (94.4 percent) hump size at cervico-thoracic positions (73.2 percent), large dewlap (62.7 percent) and long tail which is well below the hock (91.5 percent). The coat pattern of female pure-Fogera cattle is dominated by white spotted (80.3 percent) with 43.0 percent white black and 33.1 percent black white coat colour Most of the quantitative traits were highly significantly (P ≤ 0.001) affected by breed type. Except horn length and horn space all of quantitative traits for both sexes of pure-Fogera cattle from on-station were significantly (P ≤ 0.05) larger than those of the on-farm. The average linear body measurement taken on a total of 46 male pure-Fogera cattle populations were 42.68 ± 0.56 cm (mouth circumference), 16.35 ± 0.72 cm (horn length), 37.04 ± 1.16 cm (dewlap width) and 129.17 ± 1.33 cm (height at wither). The average linear body measurements for female pure-Fogera cattle were 38.23 ± 0.18 cm (mouth circumference), 13.81 ± 0.37 cm (horn length), 27.20 ± 0.42 cm (dewlap width) and 123.68 ± 0.52 cm (height at wither). The population structure were dominated by Pure-Fogera constituting 37.02 percent, Interbred with Fogera (33.71 percent) and non-Fogera (29.23 percent). The effective population size of pure-Fogera cattle was 4295, with 9016 total population. The average inbreeding level for the population was 0.012 percent. Inbreeding is at a low level and the effective population size is large. The calculated parameters indicate satisfactory genetic diversity in Fogera cattle. Milk yield, colour, power, body size and growth rate of Fogera were the most dominant traits perceived to be good by the respondents. The special qualification of this breed is to live at high amount of flooding areas with adapting other very challenging environment. Pure breeding of pure-Fogera, interbred with Fogera and non-Fogera type of breeds was used for breeding practice with natural mating. The Andassa Research Center established in 1964 as Fogera cattle population improving centre, but according to different source, population viability and population structure indicated that the population are not viable and highly admixture with other indigenous cattle breeds. According to this in order to improve the population status of Fogera cattle we recommended control with open-nucleus breeding strategy. So in order to minimize the risk status of this breed and conserve for the future generation any responsible agent should be given priority.

Knowledge of the genetic diversity of germplasm of breeding material is invaluable in crop improvement programmes. Frequently, qualitative and quantitative data are used separately to assess genetic diversity of crop genotypes. While assessing diversity based on qualitative and quantitative traits separately, there may occur a problem when the degree of correspondence between the clusters formed does not agree with each other. This study compares five different procedures of clustering based on the criterion of weighted average of observed proportion of misclassification in black gram genotypes using qualitative, quantitative traits and mixture data. The INDOMIX- and PRINQUAL-based clustering procedures, i.e. INDOMIX and PRINQUAL methods in conjunction with the k-means clustering procedure, show better performance compared with other clustering procedures, followed by clustering based on either quantitative or qualitative data alone. The use of the INDOMIX- and PRINQUAL-based procedures can help breeders in capturing the variation present in both qualitative and quantitative trait data simultaneously and solving the problem of ambiguity over the degree of correspondence between clustering based on either qualitative or quantitative traits alone.

We analysed the patterns of variation for 14 qualitative and 12 quantitative traits in 11,402 pigeonpea germplasm accessions from 54 countries, which were grouped into 11 regions. Semi-spreading growth habit, green stem colour, indeterminate flowering pattern and yellow flower colour were predominant among qualitative traits. Primary seed colour had maximum variability and orange colour followed by cream were the two most frequent seed colours in the collection. Variances for all the traits were heterogeneous among regions. The germplasm accessions from Oceania were conspicuous by short growth duration, short height, fewer branches, pods with fewer seeds, smaller seed size and lower seed yields. The accessions from Africa were of longer duration, taller, with multi-seeded pods and larger seeds. The germplasm diversity indicated by Shannon–Weaver diversity index (H′) pooled over all traits, was highest for Africa (0.464±0.039) and lowest for Oceania (0.337±0.037). The cluster analysis based on three principal component scores using 12 quantitative traits revealed formation of three clusters: cluster 1 includes accessions from Oceania; cluster 2 from India and adjacent countries; and cluster 3 from Indonesia, Thailand, the Philippines, Europe, Africa, America and the Caribbean countries. Pigeonpea-rich countries such as Myanmar, Uganda, and others like Bahamas, Burundi, Comoros, Haiti and Panama are not adequately represented in the collection, and need priority attention for germplasm exploration.

A theoretical analysis of the potential benefits of marker-assisted selection (MAS) of candidate bulls prior to entry into a young sire progeny testing programme was carried out. It is assumed that quantitative trait loci (QTL) affecting milk production have been mapped with respect to known genetic markers, and MAS is based on evaluation of elite sires in order to identify marker alleles in coupling to favourable or unfavourable QTL alleles. Candidate bulls, descendants of the elite sire will then be selected, prior to conventional progeny testing, on the basis of the marker alleles derived from the elite-sire ancestor.

The analysis considers recombination between marker and QTL, the difficulty of tracing specific marker alleles from sire to progeny, and the expectation that MAS, in practice, will be implemented in the grandsons, rather than in the sons of elite sires. It is shown that MAS of candidate bulls, based on the use of a single diallelic marker in linkage to a QTL will have only a negligible effect on the rate of genetic progress. Increases of 15 to 20% in the rate of genetic gain, however, can be obtained by the use of single polyallelic markers, and increases of 20 to 30% can be obtained by utilizing haplotypes of diallelic or polyallelic markers.