Purple nutsedge (Cyperus rotundus L.) is a globally distributed noxious weed that poses a significant challenge for control due to its fast and efficient propagation through the tuber, which is the primary reproductive organ. Gibberellic acid (GA3) has proven to be crucial for tuberization in tuberous plants. Therefore, understanding the relationship between GA3 and tuber development and propagation of C. rotundus will provide valuable information for controlling this weed. This study shows that the GA3 content decreases with tuber development, which corresponds to lower expression of bioactive GA3 synthesis genes (CrGA20ox, two CrGA3ox genes) and two upregulated GA3 catabolism genes (CrGA2ox genes), indicating that GA3 is involved in tuber development. Simultaneously, the expression of two CrDELLA genes and CrGID1 declines with tuber growth and decreased GA3, and yeast two-hybrid assays confirm that the GA3 signaling is DELLA-dependent. Furthermore, exogenous application of GA3 markedly reduces the number and the width of tubers and represses the growth of the tuber chain, further confirming the negative impact that GA3 has on tuber development and propagation. Taken together, these results demonstrate that GA3 is involved in tuber development and regulated by the DELLA-dependent pathway in C. rotundus and plays a negative role in tuber development and propagation.

The potential for yellow nutsedge (Cyperus esculentus L. var. leptostachyus Boeck. # CYPES) regrowth was reduced by increasing plant age and by glyphosate [N-(phosphonomethyl)glycine] and oxyfluorfen [2-chloro-1-(3-ethoxy-4-nitrophenoxy)-4-(trifluoromethyl)benzene]. Of the amount of glyphosate transported to underground parts, most was found in the tubers, with immature rhizomes, roots, and tubers having 2 to 3 times higher concentrations than comparable mature tissues. Addition of unlabeled oxyfluorfen as a tank mixture with glyphosate increased absorption and translocation, mainly into leaves and new tubers, of radioactivity from 14C-glyphosate in 30-day-old plants. In contrast to the younger plants, the addition of oxyfluorfen decreased 14C-glyphosate detected in 60-day-old plants. The greater effectiveness of glyphosate when applied with oxyfluorfen on yellow nutsedge at the early growth stage has important implications in the field because the tuberization process must be stopped early in the development of the plant for successful control.

Growth and morphology of two U.S. hydrilla biotypes grown under 10- and 16-h photoperiods in outdoor aquaria were investigated. The dioecious hydrilla biotype elongated extensively under both photoperiods and reached the water surface within 2 to 3 weeks after planting. In contrast, the monoecious biotype grew more densely near the sediment, primarily by producing a high number of horizontal stems, root crowns, and higher shoot densities. Monoecious hydrilla grown from tubers produced new tubers after 4 weeks of exposure to the 10-h photoperiod, and the number of tubers produced was five- to sevenfold higher in the monoecious than in the dioecious biotype. Furthermore, monoecious hydrilla produced tubers under both 10- and 16-h photoperiods, with tuber production much higher under the shorter photoperiod. Dioecious hydrilla grown from tubers under similar conditions produced new tubers after 8 weeks only under the 10-h photoperiod. No tubers were produced by the dioecious biotype throughout a 10-week exposure to the 16-h photoperiod.

Yellow nutsedge (Cyperus esculentus L.) and purple nutsedge (C. rotundus L.) were grown in clay pots in the field to investigate the effect of sunlight intensity, planting date, and harvesting date on growth and development. Reducing the length of the growing season by delayed planting or early harvesting reduced the growth (dry matter accumulation) and tuber production of both species. Purple nutsedge growth (dry matter accumulation) was linearly reduced at 30 and 73% shade, but yellow nutsedge growth at 30% shade was not different from that at full sunlight. Tuber production in both species began about August 1, with slight delays in the initiation of tuber growth as planting date was delayed. At the end of the growing season a significant number of tubers were formed in both species even at the latest planting date under 73% shade.