Hostname: page-component-6bb9c88b65-vmslq Total loading time: 0 Render date: 2025-07-24T08:52:58.503Z Has data issue: false hasContentIssue false
  • English
  • Français

Tuber development and propagation are inhibited by GA3 effects on the DELLA-dependent pathway in purple nutsedge (Cyperus rotundus)

Published online by Cambridge University Press:  04 September 2023

Chen-Yang Wei ,
Dan-Li Fan ,
Shu-Yu Liu ,
Shan-Chi Yi ,
Shi-Xian Yu ,
Guo-Chao Zhao Open the ORCID record for Guo-Chao Zhao [Opens in a new window] ,
Xiao-Liang Liu  and
Wen-Wei Tang Open the ORCID record for Wen-Wei Tang [Opens in a new window]
Chen-Yang Wei
Affiliation:
Graduate Student, Guangxi Key Laboratory of Agro-Environment and Agric-Product Safety, National Demonstration Center for Experimental Plant Science Education, College of Agriculture, Guangxi University, Nanning, Guangxi, People’s Republic of China
Dan-Li Fan
Affiliation:
Graduate Student, Guangxi Key Laboratory of Agro-Environment and Agric-Product Safety, National Demonstration Center for Experimental Plant Science Education, College of Agriculture, Guangxi University, Nanning, Guangxi, People’s Republic of China
Shu-Yu Liu
Affiliation:
Graduate Student, Guangxi Key Laboratory of Agro-Environment and Agric-Product Safety, National Demonstration Center for Experimental Plant Science Education, College of Agriculture, Guangxi University, Nanning, Guangxi, People’s Republic of China
Shan-Chi Yi
Affiliation:
Graduate Student, Guangxi Key Laboratory of Agro-Environment and Agric-Product Safety, National Demonstration Center for Experimental Plant Science Education, College of Agriculture, Guangxi University, Nanning, Guangxi, People’s Republic of China
Shi-Xian Yu
Affiliation:
Graduate Student, Guangxi Key Laboratory of Agro-Environment and Agric-Product Safety, National Demonstration Center for Experimental Plant Science Education, College of Agriculture, Guangxi University, Nanning, Guangxi, People’s Republic of China
Guo-Chao Zhao
Affiliation:
Undergraduate Student, Guangxi Key Laboratory of Agro-Environment and Agric-Product Safety, National Demonstration Center for Experimental Plant Science Education, College of Agriculture, Guangxi University, Nanning, Guangxi, People’s Republic of China
Xiao-Liang Liu
Affiliation:
Assistant Professor, Guangxi Key Laboratory of Agro-Environment and Agric-Product Safety, National Demonstration Center for Experimental Plant Science Education, College of Agriculture, Guangxi University, Nanning, Guangxi, People’s Republic of China
Wen-Wei Tang*
Affiliation:
Professor, Guangxi Key Laboratory of Agro-Environment and Agric-Product Safety, National Demonstration Center for Experimental Plant Science Education, College of Agriculture, Guangxi University, Nanning, Guangxi, People’s Republic of China
*
Corresponding author: Wen-Wei Tang; Email: wenweitg@163.com
Get access Rights & Permissions [Opens in a new window]

Abstract

Purple nutsedge (Cyperus rotundus L.) is a globally distributed noxious weed that poses a significant challenge for control due to its fast and efficient propagation through the tuber, which is the primary reproductive organ. Gibberellic acid (GA3) has proven to be crucial for tuberization in tuberous plants. Therefore, understanding the relationship between GA3 and tuber development and propagation of C. rotundus will provide valuable information for controlling this weed. This study shows that the GA3 content decreases with tuber development, which corresponds to lower expression of bioactive GA3 synthesis genes (CrGA20ox, two CrGA3ox genes) and two upregulated GA3 catabolism genes (CrGA2ox genes), indicating that GA3 is involved in tuber development. Simultaneously, the expression of two CrDELLA genes and CrGID1 declines with tuber growth and decreased GA3, and yeast two-hybrid assays confirm that the GA3 signaling is DELLA-dependent. Furthermore, exogenous application of GA3 markedly reduces the number and the width of tubers and represses the growth of the tuber chain, further confirming the negative impact that GA3 has on tuber development and propagation. Taken together, these results demonstrate that GA3 is involved in tuber development and regulated by the DELLA-dependent pathway in C. rotundus and plays a negative role in tuber development and propagation.

Keywords

tuber chaintuberizationgibberellic acidDELLA protein

Information

Type
Research Article
Information
Weed Science , Volume 71 , Issue 5 , September 2023 , pp. 453 - 461
Check for updates
Copyright
© The Author(s), 2023. Published by Cambridge University Press on behalf of the Weed Science Society of America

Access options

Get access to the full version of this content by using one of the access options below. (Log in options will check for institutional or personal access. Content may require purchase if you do not have access.)

Article purchase

Temporarily unavailable

Footnotes

Associate Editor: William Vencill, University of Georgia

References

Bendixen, LE, Nandihalli, UB (1987) Worldwide distribution of purple and yellow nutsedge (C. rotundus and C. esculentus). Weed Technol 1:61–65 CrossRefGoogle Scholar
Bou-Torrent, J, Martínez-García, JF, García-Martínez, JL, Prat, S, Blazquez, MA (2011) Gibberellin A1 metabolism contributes to the control of photoperiod-mediated tuberization in potato. PLoS ONE 6:e24458 CrossRefGoogle Scholar
Carrera, E, Bou, J, García Martínez, JL, Prat, S (2000) Changes in GA 20-oxidase gene expression strongly affect stem length, tuber induction and tuber yield of potato plants. Plant J 22:247–256 CrossRefGoogle Scholar
Chen, P, Yang, R, Bartels, D, Dong, T, Duan, H (2022) Roles of abscisic acid and gibberellins in stem/root tuber development. Int J Mol Sci 23:4955 CrossRefGoogle ScholarPubMed
Cheng, L, Wang, Y, Liu, Y, Zhang, Q, Gao, H, Zhang, F (2018) Comparative proteomics illustrates the molecular mechanism of potato (Solanum tuberosum L.) tuberization inhibited by exogenous gibberellins in vitro. Physiol Plant 163:103–123 CrossRefGoogle ScholarPubMed
Chetram, RS, Bendixen, LE (1974) Gibberellic acid plus cytokinins induced basal bulbs of purple nutsedge above ground. Weed Sci 22:55–58 CrossRefGoogle Scholar
Davière, J, Achard, P (2013) Gibberellin signaling in plants. Development 140:1147–1151 CrossRefGoogle ScholarPubMed
Gazara, RK, Moharana, KC, Bellieny-Rabelo, D, Venancio, TM (2018) Expansion and diversification of the GIBBERELLIN RECEPTOR GIBBERELLIN INSENSITIVE DWARF1 (GID1) family in land plants. Plant Mol Biol 97:435–449CrossRefGoogle Scholar
Gong, M, Luo, H, Wang, A, Zhou, Y, Huang, W, Zhu, P, He, L (2017) Phytohormone profiling during tuber development of Chinese yam by ultra-high performance liquid chromatography-triple quadrupole tandem mass spectrometry. J Plant Growth Regul 36:362–373 CrossRefGoogle Scholar
He, Z, Zhang, H, Gao, S, Lercher, MJ, Chen, W, Hu, S (2016) Evolview v2: an online visualization and management tool for customized and annotated phylogenetic trees. Nucleic Acids Res 44:W236–W241 CrossRefGoogle ScholarPubMed
Hedden, P, Sponsel, V (2015) A century of gibberellin research. J Plant Growth Regul 34:740–760 CrossRefGoogle ScholarPubMed
Hedden, P, Thomas, SG (2012) Gibberellin biosynthesis and its regulation. Biochem J 444:11–25 CrossRefGoogle ScholarPubMed
Horowitz, M (1972) Growth, tuber formation and spread of Cyperus rotundus L. from single tubers. Weed Res 12:348–363 CrossRefGoogle Scholar
Kloosterman, B, Navarro, C, Bijsterbosch, G, Lange, T, Prat, S, Visser, RGF, Bachem, CWB (2007) StGA2ox1 is induced prior to stolon swelling and controls GA levels during potato tuber development. Plant J 52:362–373 CrossRefGoogle ScholarPubMed
Kolachevskaya, OO, Lomin, SN, Kojima, M, Getman, IA, Sergeeva, L, Sakakibara, H, Romanov, GA (2019) Tuber-specific expression of two gibberellin oxidase transgenes from Arabidopsis regulates over wide ranges the potato tuber formation. Russ J Plant Physiol 66:984–991 CrossRefGoogle Scholar
Larridon, I, Bauters, K, Reynders, M, Huygh, W, Muthama Muasya, A, Simpson, DA, Paul, G (2013) Towards a new classification of the giant paraphyletic genus Cyperus (Cyperaceae): phylogenetic relationships and generic delimitation in C4 Cyperus . Bot J Linn Soc 172:106–126 CrossRefGoogle Scholar
Livak, KJ, Schmittgen, TD (2001) Analysis of relative gene expression data using real-time quantitative PCR and the 2−ΔΔCT method. Methods 25:402–408 CrossRefGoogle Scholar
Murase, K, Hirano, Y, Sun, T, Hakoshima, T (2008) Gibberellin-induced DELLA recognition by the gibberellin receptor GID1. Nature 456:459–463 CrossRefGoogle ScholarPubMed
Peerzada, AM (2017) Biology, agricultural impact, and management of Cyperus rotundus L.: the world’s most tenacious weed. Acta Physiol Plant 39:1–14 CrossRefGoogle Scholar
Potter, SC, Luciani, A, Eddy, SR, Park, Y, Lopez, R, Finn, RD (2018) HMMER web server: 2018 update. Nucleic Acids Res 46:W200–W204 CrossRefGoogle ScholarPubMed
Roumeliotis, E, Kloosterman, B, Oortwijn, M, Lange, T, Visser, RGF, Bachem, CWB (2013) Down regulation of StGA3ox genes in potato results in altered GA content and affect plant and tuber growth characteristics. J Plant Physiol 170:1228–1234 CrossRefGoogle ScholarPubMed
Takeshi, I, Kanako, O, Fukazawa, J, Takahashi, Y (2018) DELLA-dependent and -independent gibberellin signaling. Plant Signal Behav 13:e1445933 Google Scholar
Tamura, K, Stecher, G, Kumar, S (2021) Mega11: molecular evolutionary genetics analysis version 11. Mol Biol Evol 38:3022–3027 CrossRefGoogle ScholarPubMed
Wang, G, Mcgiffen, ME Jr, Ogbuchiekwe, EJ (2008) Crop rotation effects on Cyperus rotundus and C. esculentus population dynamics in southern California vegetable production. Weed Res 48:420–428 CrossRefGoogle Scholar
Webster, TM, Grey, TL (2014) Halosulfuron reduced purple nutsedge (Cyperus rotundus) tuber production and viability. Weed Sci 62:637–646 CrossRefGoogle Scholar
Webster, TM, Grey, TL, Davis, JW, Culpepper, AS (2008) Glyphosate hinders purple nutsedge (Cyperus rotundus) and yellow nutsedge (Cyperus esculentus) tuber production. Weed Sci 56:735–742 CrossRefGoogle Scholar
Wills, GD (1987) Description of purple and yellow nutsedge (Cyperus rotundus and C. esculentus). Weed Technol 1:2–9 CrossRefGoogle Scholar
Woffelman, C (2004) DNAMAN for windows. Leiden, Netherlands: Leiden University, Lynnon Biosoft, and the Institute for Molecular Plant Sciences Google Scholar
Wu, L, Lan, J, Xiang, X, Xiang, H, Jin, Z, Khan, S, Liu, Y (2020) Transcriptome sequencing and endogenous phytohormone analysis reveal new insights in CPPU controlling fruit development in kiwifruit (Actinidia chinensis). PLoS ONE 15:e240355 CrossRefGoogle ScholarPubMed
Xu, X, van Lammeren, AAM, Vermeer, E, Vreugdenhil, D (1998) The role of gibberellin, abscisic acid, and sucrose in the regulation of potato tuber formation in vitro. Plant Physiol 117:575–584 CrossRefGoogle ScholarPubMed
Yogev, M, Rubin, B, Negbi, M (1996) Hormonal induced transformation of the tuberous purple nutsedge (Cyperus rotundos L.) into a tuber-less phenotype. Isr J Plant Sci 44:19–28 CrossRefGoogle Scholar
Zhou, Y, Li, Y, Gong, M, Qin, F, Xiao, D, Zhan, J, Wang, A, He, L (2021) Regulatory mechanism of GA3 on tuber growth by DELLA-dependent pathway in yam (Dioscorea opposita). Plant Mol Biol 106:433–448 CrossRefGoogle ScholarPubMed
Zierer, W, Rüscher, D, Sonnewald, U, Sonnewald, S (2021) Tuber and tuberous root development. Annu Rev Plant Biol 72:551–580 CrossRefGoogle ScholarPubMed
Supplementary material: File

Wei et al. supplementary material

Tables S1-S4 and Figure S1

Download Wei et al. supplementary material(File)
File 5.1 MB