This study reports the conodont succession across the Visean–Serpukhovian (V/S) boundary interval at the Naqing section, South China. Continuous centimetre-scale sampling of the relatively deep-water section in recent years has provided new data for a more detailed biostratigraphy of conodonts across the Visean–Serpukhovian boundary. Three conodont zones were described in ascending order: the Gnathodus bilineatus, Lochriea nodosa and Lochriea ziegleri zones. The first appearance datum (FAD) of L. ziegleri has been moved down to 60.1 m above the base of the Naqing section. The correlation of the conodont succession across the Visean–Serpukhovian boundary in the Naqing section with other sections in Eurasia is discussed.