Cowpea is a major specialty crop in the southern US. In recent years, no new herbicide programs have been evaluated for cowpea despite additional herbicide registrations. Studies were conducted from 2014 to 2016 at Fayetteville and Kibler, Arkansas to assess new herbicide programs for cowpea production. The herbicide programs included: three commercial standard programs; fomesafen (PPL, 0.21 kgha−1)-, flumioxazin (PPL, 0.21 kgha−1)-, and halosulfuron (PPL, 0.054 kgha−1)-based programs with or without S-metolachlor (1.12 kgha−1) fb imazethapyr (0.07 kgha−1); and two sets of sulfentrazone (PPL/PRE)-based programs applied alone (0.21 kgha−1) or as a pre-mixture with carfentrazone (0.11 kgha−1+0.01 kgha−1) with or without S-metolachlor (1.12 kgha−1). The sulfentrazone-based programs included POST applications of imazethapyr fb sethoxydim (0.32 kgha−1) or fluthiacet-methyl (0.0067 kgha−1) and sethoxydim as necessary. In 2014 and 2015, crop stand loss was minimal and crop injury was generally low (<20%). Weed control from sulfentrazone- and flumioxazin-based programs was excellent (>90%). In 2016, with heavy rainfall around planting time, sulfentrazone-containing programs reduced cowpea yield 45% to 60%. Flumioxazin-based programs caused >85% injury at Kibler early-season, which lasted until harvest. Heavy rainfall also reduced efficacy of residual herbicides. In general, the sulfentrazone- and flumioxazin-based treatments consistently yielded similar to the weed-free controls. The majority of the programs had <60% weed control in Fayetteville early in the season. POST herbicides improved weed control to >90% in most treatments. Palmer amaranth and annual grass control was generally better in Kibler, with >80% control at harvest. Sulfentrazone is registered for cowpea and is effective on Palmer amaranth, but growers need to be careful about where and when to use it. Flumioxazin should be considered for registration in cowpea once its use pattern and location-specific recommendations are well defined.

Intercropping with functionally diverse crops can reduce the availability of resources that could otherwise be used by weeds. An experiment was conducted across 6 site-years in New York and Maryland in 2013 and 2014 to examine the effects of functional diversity and crop species richness on weed suppression. We compared four annual crop species that differed in stature and nitrogen acquisition traits: (1) pearl millet, (2) sorghum sudangrass, (3) cowpea, and (4) sunn hemp. Crops were seeded in monoculture and in three- and four-species mixtures using a replacement design in which monoculture seeding rates were divided by the number of species in the intercrop. Crop and weed biomass were sampled at ~45 and 90 d after planting. At the first sampling date, intercrops produced more crop biomass than monocultures in all but 1 site-year; however, weed biomass in intercrops was lower than monocultures in only 1 site-year. By the second sampling date, crop biomass was consistently greater in the intercrops than in the monocultures, and weed biomass was lower in the intercrops than in monocultures in 2 site-years. Although we observed several negative relationships between crop species richness and weed biomass, crop biomass was a more important factor than species richness for suppressing weeds. Despite the weak weed suppression from the two legumes compared with the two grasses, legume crops can provide other benefits, including increased forage quality, soil nitrogen for subsequent crops, and resources for pollinators if allowed to flower. On the other hand, if weed suppression is the top priority, our results suggest that monocultures of high biomass–producing grasses will provide more effective suppression at a lower seed cost than functionally diverse intercrops that include low biomass–producing legumes in warm-season intercrops.

N fertilizers suppress witchweed plant growth and development, thus reducing the severity of parasite attack and increasing host yield simultaneously. However, the underlying physiological mode of N action occurring within the parasite cells remains largely unknown. This study aims at screening for the effects of N forms and different growth conditions on some N assimilation enzymes in witchweed seedlings grown aseptically without host plant, and in pots with host plants. Results show that supply of N in NH4 + or urea forms resulted in 83 to 92% reduction in nitrate reductase activity (NRc), compared with control. Increasing NO3 − concentrations from 0 mM to 100 mM, led to a corresponding increase in NRc in giant witchweed. NRc of giant witchweed seedlings grown under light and dark cycles were about 270 times higher than seedlings grown in continuous darkness. A combination of NH4 + and NO3 −, resulted in increased giant witchweed NRc, compared with NH4 + or NO3 − supplied singly. Highest shoot development and NRc was at NH4 + and NO3 − ratio 1:1, followed by ratios 1:3, 3:1, 0:1, and 1:0, respectively. Addition of N in soils resulted in increased NRc, followed by rapid deterioration and death of giant witchweed plants. NRc, GSc, and GDHc in witchweed, maize, cowpea, and tobacco were affected by diurnal fluctuations with higher enzyme activities occurring during the day than at night. Higher GSc than GDHc suggests that NH4 + assimilation occurs mainly through the GS pathway in witchweed plants. NRc and GDHc were two and four times higher in giant witchweed grown in aseptic media without host plant, than that grown in potted soils with host plants. These findings provide insight into the physiological mode of N action and their implications on witchweed control.

The U. S. Plant Introduction Collection of cowpea germplasm and a collection of cowpea cultivars were evaluated for bentazon tolerance in the field. Most accessions were intermediate in bentazon tolerance; however, several highly tolerant and highly susceptible accessions were identified. Tolerant and susceptible selections from the field experiment were evaluated in greenhouse experiments. The most susceptible selections were killed or severely injured by bentazon at 2 kg ae ha−1; the most tolerant selections were not severely injured by bentazon at 16 kg ha−1.

Tests with 18 growth regulators and chemicals revealed that the cytokinins [zeatin, kinetin, and 6-benzylaminopurine (BAP)], ethylene, and sodium hypochlorite stimulate germination of cowpea witchweed seeds at various concentrations. Witchweed seeds preconditioned in different concentrations of sodium hypochlorite for 3 wk had germination rates of 25 to 89% after incubation in distilled water, 10−5M solution of kinetin, 50 mg L−1 of 2-chloroethylphosphonic acid, and exudates from cowpea roots. Witchweed seeds preconditioned in distilled water and subsequently incubated in distilled water did not germinate.

Bioassay experiments were conducted to determine the phytotoxicity of methanol and ethyl acetate extracts of hairy vetch and cowpea residues on the germination and radicle elongation of three vegetable crops and three weed species. The species tested included common chickweed, redroot pigweed, wild carrot, tomato, corn, and cucumber. The extracts of both species were dissolved in methanol to yield seven concentrations ranging from 0 to 8 g/L. Germination was significantly reduced by methanol and ethyl acetate extracts of hairy vetch extracts except for corn and tomato. Common chickweed and wild carrot were the only species that showed consistent reduction in germination with the methanol and ethyl acetate cowpea extracts. The radicle growth of most species, with the exception of corn and cucumber, was reduced by the extracts of both cover crops. Corn and cucumber radicle elongation was stimulated at low concentrations of the extracts; however, these observations were not significantly different among treatments. This study demonstrated that methanol and ethyl acetate extracts of hairy vetch and cowpea contained allelopathic compounds and that their phytotoxicity is likely species specific. Future studies should focus on the identification and isolation of the allelochemical(s) found in the methanol and ethyl acetate extracts of the hairy vetch and cowpea residues.

Canada thistle is a perennial spreading weed that is difficult to control in farming systems with reduced reliance upon herbicides for weed management. Experiments were conducted from 2006 to 2008 at Champaign, IL, to evaluate the combined effects of summer annual cover crops and mowing on Canada thistle growth and survival. Whole plot treatments were fallow, buckwheat, sudangrass–cowpea mixture (MIX), and sudangrass. The subplot treatments were mowing frequencies (0 to 2 times). Cover crop and mowing did not interact to suppress Canada thistle. MIX and sudangrass produced more standing biomass, greater regrowth, and more surface mulch following mowing than the buckwheat. A single season with sudangrass or MIX reduced Canada thistle shoot density and mass to less than 20% of the initial values through two growing seasons. Mowing alone only suppressed Canada thistle shoot density and mass on the site with greater initial density. A sudangrass or MIX cover crop alone or combined with mowing suppresses Canada thistle, but intensive management must continue for several years to eliminate patches.

Greenhouse replacement-series experiments were conducted to evaluate the competitiveness of cowpea, sunn hemp, and velvetbean when grown in combination with yellow nutsedge and smooth pigweed. The effect of the cover crop species on yellow nutsedge tuber production was also evaluated. Cowpea and velvetbean were equally competitive with yellow nutsedge, but sunn hemp was less competitive. Although sunn hemp height was double that of cowpea or velvetbean, photosynthetically active radiation penetrating to the soil surface was twofold to eightfold greater than with the other two species. Leaf area per plant with sunn hemp monocultures were only 63 to 70% of cowpea and 37 to 41% of velvetbean. Increasing the proportion of cover crops in crop : weed mixtures did not significantly affect nutsedge tuber number per plant or tuber weight per plant. Cowpea was more competitive than smooth pigweed, whereas both sunn hemp and velvetbean were less competitive than smooth pigweed. The utility and efficacy of leguminous cover crop species for nutsedge and smooth pigweed suppression do not appear to be due to inherent competitiveness. Until cultivars that are more competitive become available, cultural measures should be employed that enhance cover crop modification of soil environmental conditions to minimize weed seed germination and vegetative propagule sprouting.