Use of Patagonia's forests by hunter-gatherers remains relatively poorly understood. Regional archaeological records indicate initial forest colonization during the middle Holocene, infrequent use until late prehistory, and, even then, fairly sparse occupation, likely in conjunction with use of the adjacent steppe. Recent excavations at Río Ibáñez-6 West in Aysén, Chile, provide a new perspective for understanding the prehistoric use of Patagonian forests, particularly regarding timing of the initial occupation and the potential for development of a forest-specific adaptation. We provide chronological, lithic, archaeofaunal, and macrobotanical data that show use of the Ibáñez River valley at least a millennium earlier than previously documented. These data indicate increased dietary breadth in late prehistory, supporting the established hypothesis that the valley became a closed system at that time. From our data we develop hypotheses regarding prehistoric forest use in Aysén that have implications for the broader understanding of the suitability of the forest for prehistoric human foragers.