Alfalfa seed producers have a limited number of herbicide options to manage weed problems. MON-37500 (proposed name sulfosulfuron) is a sulfonylurea herbicide that controls dandelion and quackgrass, two common weeds in alfalfa fields. A study was conducted in two alfalfa fields at Valparaiso and Carrot River, Saskatchewan, Canada, from 1999 to 2001 to evaluate perennial weed control and alfalfa production responses with 0.5×, 1×, and 1.5× label-recommended rates of MON-37500 and also 2,4-DB and hexazinone. MON-37500 applied at the 1× and 1.5× rates at both locations reduced mid-May alfalfa vigor from 100% to between 80 and 90% and increased early-season control of dandelion and quackgrass by about 10 to 40 percentage units, when compared with other herbicide treatments. Improved weed control with 1× and 1.5× MON-37500 rates was sustained into mid-June only at Carrot River and was completely eliminated (100% vigor and 0% weed control), or almost so, by mid-July. MON-37500 did not control Canada thistle. Improved early-season weed control with the 1× MON-37500 rate apparently compensated for the loss of alfalfa vigor at Valparaiso, thus resulting in 27% (57 kg/ha) greater seed yield than with the other herbicide treatments. At Carrot River, hexazinone generally provided levels of weed control similar to MON-37500 but did not injure alfalfa. Consequently, alfalfa yields were highest and the proportion of dead (decaying) seed was least with this treatment. The 0.5× MON-37500 rate often resulted in inferior weed control relative to the 1× and 1.5× rates and never was among the herbicide treatments providing the greatest seed yield. Managing the residual activity of MON-37500 and its negative effect on alfalfa growth, especially at locations with soils having coarse texture and low organic matter content, represents the greatest challenge in making MON-37500 a reliable weed management tool for alfalfa seed producers.