Skip to main content Accessibility help

We use cookies to distinguish you from other users and to provide you with a better experience on our websites. Close this message to accept cookies or find out how to manage your cookie settings.

Close cookie message
×
  1. Home
  2. Search

Refine search

Refine search

Access:
Content type:
Publication date:
Apply   From and To filters
Subject: Show more
Journals Show more
Publishers: Show more
Societies Show more
Series: Show more
Collections: Show more

Actions for selected content:

Select all | Deselect all
  • View selected items
  • Export citations
  • Download PDF (zip)
  • Save to Kindle
  • Save to Dropbox
  • Save to Google Drive

Save Search

You can save your searches here and later view and run them again in "My saved searches".

Please provide a title, maximum of 40 characters.
Cancel
×

1 results

  • Cryo-Planing Biological Specimens for Scanning Electron Microscopy

  • William C. Plumley, Michael Kostrna, Steven L. Goodman
  • Journal:
    Microscopy Today / Volume 27 / Issue 2 / March 2019
    Published online by Cambridge University Press:
    14 March 2019, pp. 20-25
    Print publication:
    March 2019
    • Article
      • You have access
    • PDF
    • HTML
    • Export citation

  • Cryo-planing SEM provides a powerful 3D view of internal and external tissue and cell features. However, specimen preparation is tedious because it requires custom apparatus and difficult cryogenic manipulations. We present an easy method using mPrep/s capsules that provide a “handle” to hold and orient specimens throughout cryo-preparation and imaging. Cryo-facing is done with a cryo-ultramicrotome, requiring no custom equipment while providing accurate control of the image plane. We show this with fresh Christmas cactus leaves and aldehyde-fixed kidney tissue imaged by room temperature SEM after freeze-drying, demonstrating the method operating without an expensive cryo-SEM.