Safflower, a semiarid crop, contains a healthy oil with high unsaturated fatty acids. Genetically diverse accessions are important for genetic maintenance of safflower and breeding proposes. The objectives of present investigation were to evaluate the morphological variation of 100 safflower accessions across two years (2022 and 2023), to explore similar genotypic groups and to identify the higher contribution of traits with to the observed variability. The highest coefficient of variation (CV) was observed for seeds per secondary capitulum, number of capitula per plant and weight of lateral capitulum in the first year and the highest CV values were observed for number of capitula per plant and capitula per lateral branch in the second year. The factor analysis identified five factors in the first year and six factors in the second as yield components, height, seed yield, capitulum diameter and phonology while number of branches was identified as the extra factor in the second year. Results showed that the variation of morphologic traits was made up of from the most measured traits of safflower. We defined seven distinct clusters, which made it possible to differentiate safflower accessions based on measured traits across two years. Of 45 accessions were grouped in similar clusters across two years, without any or similar genotype by environment interaction. Some high yielding accessions like C-47 and Lesaf-175 can be entered directly in multi-environmental trials for cultivar release proposes. The recognized variation improves as a good resource, indicating an important issue for future projects for safflower germplasm maintenance and breeding.

Safflower grown in the western U.S. is often produced for birdseed mixes. Increasing demand for birdseed products, combined with regional drought, has shrunk western safflower availability. To satisfy the growing demand, processors may look to contracting strategies to incentivize production. We compare expected risk and corresponding certainty equivalents both from the processor and producer viewpoints under various contracting mechanisms and risk aversion levels. Results suggest that contracts containing a combination of lump sum acreage payments and fixed price performance payments would incentivize producer adoption of safflower while maintaining processor profitability and limiting the risk exposure of both parties.

Understanding the reasons for the yield gap between potential and actual yield can provide insights for enhancing canola production by adapting measures for ensuring food security. The canola yield gap under different management practices (e.g. water, nitrogen, N- and sowing dates) was quantified using research trials that were conducted at on-station and historical data (1980–2016) and the CROPGRO-Canola model for Punjab, Pakistan. The integrated approach revealed that low inputs of N, the amount of irrigation, sowing date and the use of seeds from home stocks were the principal causes for a low yield. The CROPGRO-Canola model was able to simulate the canola yield from research trials (R2 = >0.90) and farm survey data (R2 = 0.63). The average yield gap between potential (YP), N-limited (YNL), water-limited (YWL), N- and water-limited (YNWL), and overall farmer field yield (YOFF) was 50, 46, 62 and 72%, respectively. The yield-gap with achievable yield (YA) for YNL, YWL, YNWL and YOFF was 34, 28, 49 and 63%, respectively. Overall, the results showed that a high canola yield for farmers’ fields can be obtained by selecting appropriate varieties and sowing dates with N rate of 120 kg/ha and efficient irrigation management. However, further studies are necessary to fully comprehend the underlying causes for the low actual yield and the high yield variability of farmers’ fields.

This research communication describes the influence of diet, mammary quarter position and milking process on the temperature of teats and udder of cows fed diets containing different lipid sources. Five primiparous cows were fed diets containing cottonseed, sunflower seed, soybeans or soybean oil as a source of lipids and a reference diet without the inclusion of lipid sources in a 5 × 5 Latin Square design. Milk yield was determined in the last five days of each period. Milk samples were collected for SCC analysis on the last two days of each experimental period. The images of the mammary gland were obtained using an infrared camera and were analyzed with appropriate computer software. Milk yield was 14.8% higher for cows fed soybeans as a source of lipids. Diets and somatic cell counts did not influence the temperature of teats and udder. The milking process reduced the temperature of teats and udder by 0.79°C. Rear teats and rear quarters had higher surface temperatures than front teats and fore quarters. Changes in temperature of teats and mammary quarters occurred as a function of the milking process and quarter position. However, the diet and the SCC did not influence the temperature of teats and mammary quarters in this experiment.

The mitigation of enteric methane emission in beef cattle production is important for reducing feed energy loss and increasing environmental sustainability. The main objective of this study was to evaluate the effect of different oilseeds included in fermented total mixed rations (whole soyabean seed (SBS, control), whole kapok seed (KPS) and cracked oil palm fruit (OPF)) on feed intake, digestibility, rumen microbial populations, energy partition and methane emissions in different cattle genotypes (Charolais crossbred v. Japanese Black crossbred). Three Charolais crossbred and three Japanese Black crossbred bulls were studied in a replicated 3×3 Latin square experimental design; genotypes were analysed in separate squares including three periods of 21 days each and three dietary oilseed treatments fed ad libitum. The cattle were placed in a metabolic cage equipped with a ventilated head box respiration system for evaluating digestibility and energy balance. As compared with Charolais crossbred individuals, Japanese Black crossbred bulls showed consistently lower dry matter intake (15.5%, P<0.01), metabolisable energy (ME) intake (13.8%, P<0.05), ME requirement for maintenance (10.3%; 386 v. 430 kJ/kg metabolic BW, respectively), faeces energy loss (19.2%, P<0.001) and enteric methane emissions (18.5%, P<0.001). However, these two genotypes did not differ in energy retention (ER) (P=0.80). Among the three dietary oilseed treatments, OPF exhibited higher NDF intake (P<0.01) and digestibility (P<0.01), which was associated with a larger (P<0.05) total number of bacteria in the rumen. In addition, the OPF diet contributed to higher ME intake and ER than that of the KPS diet, whereas the SBS diet presented intermediate values (P<0.05). The methane conversion factor of these crossbreds was not significantly affected by genotype (P>0.05) or diet (P>0.05) under the experimental conditions and ranged from 5.8% to 6.0% of gross energy intake. This value is lower than that reported by the Intergovernmental Panel on Climate Change (6.5%) for cattle fed with low-quality crop residues or by-products. Thus, our results imply that the Japanese Black crossbred cattle consume less feed and emits less enteric methane than the Charolais crossbred does, mainly owing to its lower ME requirement for maintenance. The OPF diet could be used to replace SBS for high beef production, although further studies are required to evaluate their application across a wide range of beef production systems.

Calendula is an alternative oilseed crop whose seed oil is valued as a substitute for tung oil and a replacement for petroleum-based volatile organic compounds in paints and other coatings. Calendula tolerances to most POST-applied herbicides are unknown. Two POST-applied herbicides were tested for tolerance by calendula. Imazamethabenz at 0.44 kg ai ha−1 plus surfactant and desmedipham plus phenmedipham at 0.36 + 0.36 kg ai ha−1 were tolerated by calendula, but the latter herbicide must be applied after the four–leaf-pair stage of growth to avoid severe injury. Neither herbicide adversely affected calendula seed yield if applied at the four–leaf-pair stage. Because these herbicides can control several weed species, calendula tolerance to them may encourage more growers and crop advisors to test this new oilseed crop on commercial farms.

Cuphea is a new crop of temperate regions that produces seed oil that can substitute for imported coconut and palm kernel oils. Only four herbicides are known to be tolerated by cuphea to date. More herbicides, especially POST products, are needed for continued commercialization. In Minnesota and North Dakota, where cuphea currently is grown, greater control of Canada thistle and biennial wormwood is needed in cuphea. Because clopyralid is effective on both of these species, it was tested at rates ranging from about 25 to 850 g ae ha−1 in greenhouse and field trials. Visual assessment of injury, height, growth, and seed yield of cuphea were not reduced significantly in field-grown plants when clopyralid was applied at rates up to 400 g ae ha−1. Thus, at the rate commonly used in other crops, 200 g ae ha−1, clopyralid can be applied safely to cuphea.

Partial seed retention line #23(‘PSR23’) cuphea is a hybrid of Cuphea viscosissima × C. lanceolata. It is a new, spring-planted, annual, potential oilseed crop that is highly susceptible to interference by weeds because of its slow growth during spring and early summer. Grass weeds are controlled easily in this broadleaf crop, but broadleaf weeds are an appreciable problem. Consequently, several broadleaf herbicides were screened for tolerance by ‘PSR23’ cuphea. Broadleaf herbicides to which cuphea showed tolerance in a spray cabinet and a greenhouse were tested in a field setting for 2 yr. Field tolerance was considered as absence of negative impact (P > 0.05) both years to any of four measured traits: overall vigor, dry weight, stand density, and time to anthesis. Cuphea showed tolerance in the field to three soil-applied herbicides (ethalfluralin, isoxaflutole, and trifluralin) and one postemergence herbicide (mesotrione). A few combinations of soil-applied and postemergence herbicides did not damage cuphea. These combinations were ethalfluralin followed by (fb) mesotrione, isoxaflutole fb imazethapyr, and isoxaflutole fb mesotrione. Availability of these herbicides for use in cuphea production may facilitate the domestication and acceptance of this new crop.

Safflower is a traditional oilseed crop in the world. Its seed oil is a healthy edible oil containing high amount of unsaturated fatty acids. Genetically diverse exotic cultivars are valuable germplasm for introducing new diversity in safflower improvement programmes. In this study, we characterized safflower cultivars of India (30) and Mexico (23) comprising varieties, hybrids and advanced lines developed over 50 years for genetic distinctiveness using 38 simple sequence repeat (SSR) loci. Genetic diversity estimates across cultivar groups (total, India and Mexico) were as follows: mean number of alleles (3.2, 3.1, 2.6), expected heterozygosity (0.42, 0.37, 0.37) and polymorphism information content (0.36, 0.33, 0.32) respectively, which suggested narrow SSR allelic diversity within and between cultivar groups. However, distance-based cluster analysis (neighbour-joining tree) and model-based STRUCTURE analysis revealed that safflower cultivars of India and Mexico, with the exception of a few, form two genetically distinct groups. High level of genetic variation explained between the populations (40%) and Fst estimate (0.4) suggested that the cultivar groups were highly differentiated with limited gene flow supporting a strong genetic structuring. High oil (~38%) and high oleic (73–79%) contents of a subset of Mexican safflower varieties and advanced lines were confirmed in field trials in India. These exotic sources from Mexico are valuable for safflower breeding programmes in India to develop new cultivars with high oil yielding potential and high oleic acid content, which is the current market demand.

Oilseeds offer some protection to the access of ruminal microorganisms and may be an alternative to calcium salts of fatty acids (FA), which are not fully inert in the ruminal environment. This study aimed to evaluate the effects of different sources of FA supplementation on apparent total tract nutrient digestibility, milk yield and composition, and energy balance (EB) of cows during the transition period and early lactation. We compared diets rich in C18:2 and C18:3 FA. Multiparous Holstein cows were randomly assigned to receive one of the four diets: control (n=11); whole flaxseed (WF, n=10), 60 and 80 g/kg (diet dry matter (DM) basis) of WF during the prepartum and postpartum periods, respectively; whole raw soybeans (WS, n=10), 120 and 160 g/kg (diet DM basis) of WS during the prepartum and postpartum periods, respectively; and calcium salts of unsaturated fatty acids (CSFA, n=11), 24 and 32 g/kg (diet DM basis) of CSFA during the prepartum and postpartum periods, respectively. Dry cows fed WF had higher DM and net energy of lactation (NEL) intake than those fed WS or CSFA. The FA supplementation did not alter DM and NDF apparent total tract digestibility, dry cows fed WF exhibited greater NDF total tract digestion than cows fed WS or CSFA. Feeding WS instead of CSFA did not alter NEL intake and total tract digestion of nutrients, but increased milk fat yield and concentration. Calculated efficiency of milk yield was not altered by diets. FA supplementation increased EB during the postpartum period. Experimental diets increased long-chain FA (saturated and unsaturated FA) in milk. In addition, cows fed WS and CSFA had higher C18:1 trans-11 FA and C18:2 cis, and lower C18:3 FA in milk than those fed WF. Furthermore, cows fed CSFA had higher C18:1 trans-11 and cis-9, trans-11 FA than cows fed WS. Although supplemental C18:2 and C18:3 FA did not influence the milk yield of cows, they positively affected EB and increased unsaturated long-chain FA in milk fat.

This study investigated effects of roasted or extruded oilseed supplementation ranging in n-6/n-3 ratios from 0.3 to 5.0 on the fatty acid composition and expression of delta-5 desaturase (Δ5d) and Δ6-desaturase (Δ6d) protein in commercial steer cheek (m. masseter) and diaphragm (pars costalis diaphragmatis) muscles. In general, the n-6/n-3 ratio of the diet had a subsequent effect on the muscle n-6/n-3 ratio (P < 0.05), with muscle 18:2n-6 and 18:3n-3 content relating to proportion of dietary soya bean and linseed (P < 0.01). Compared with canola, pure linseed and soya bean diets reduced 14:1c-9 and 16:1c-9 (P < 0.05) but increased 18:1t-11 and c-9,t-11 conjugated linoleic acid (CLA) content (P < 0.01). Oilseed processing had a minor influence but extruded oilseeds increase 18:1t-11 and c-9,t-11 CLA compared with roasted (P < 0.05). Polar lipid 18:3n-3 and n-3 long-chain polyunsaturated fatty acid (LC, ⩾20 carbons PUFA) derivative content increased in relation to dietary linseed supplementation in the diaphragm (P < 0.01), whereas only 18:3n-3 was increased in the cheek (P < 0.01). Protein expression did not differ between diets; however, in each muscle the Δ5d protein expression had a stronger association with the desaturase products rather than the precursors. The relationship between Δ5d protein expression and the muscle LC n-6/n-3 ratio was negative in both muscles (P < 0.05). The relationship between Δ6d protein expression and the LC n-6/n-3 ratio was positive in the cheek (P < 0.001) and negative in the diaphragm (P < 0.05). In conclusion, diet n-6/n-3 ratio affected muscle 18:2n-6 and 18:3n-3 deposition, whereas the Δ5d and Δ6d protein expression had some influence on the polar lipid LC-PUFA profile. Results reaffirm that processed oilseeds can be used to increase the proportion of fatty acids potentially beneficial for human health, by influencing the formation of LC-PUFA and reducing the n-6/n-3 ratio.

Oilseed crops are major sources of oils for human nutrition, and an increasing proportion is also being utilized for industrial purposes. Recent advances in our understanding of the basic biochemistry of seed oil biosynthesis, coupled with identification of genes for oilseed modification, have set the stage for the genetic engineering of oilseed crops that produce ‘designer’ plant seed oils tailored for specific applications. In this review we provide an overview of seed oil biosynthesis and highlight the enzymatic steps that have already been targeted for genetic manipulation, with the end goal of producing seed oils containing desired amounts of fatty acid components. Furthermore, we describe the identification of genes from various wild plant species that are capable of producing structurally diverse fatty acids, and how these advances open the door to the production of entirely novel oils in conventional oilseed crops. Transgenic oilseeds producing high amounts of these novel fatty acids represent renewable sources of raw materials that may compete with, and eventually replace, some petrochemicals that are derived from non-renewable crude oil.

Small-scale oil expelling is a natural component of agricultural systems in the poorer African and Asian countries. This paper relates to the work of the Natural Resources Institute and the assistance it has given to the installation and operation of small-scale oilseed expellers in a number of countries including Zambia, Gambia and Sudan. A concept of integrated agricultural development is outlined comprising oilseed cultivation, small-scale expelling and poultry production. Consideration is given to differences in nutritive value of the oilseed meals produced, oilseed processing technology and the need for interdisciplinary research, coupled with co-ordinated strategies, in order to maximize the impact on farming communities which have desperately few resources.