The monogenean (platyhelminth) skin parasite Entobdella soleae
from the common sole (Solea solea) lays tetrahedral eggs.
One of the 4 corners of the tetrahedron is a detachable operculum which
is bonded to the rest of the egg-shell by cement.
Most of this cement layer, beginning at the inner surface of the shell
and running through almost to the outer surface (a
distance of about 2 μm), is more or less uniform in thickness (30–38
nm), or tapers slightly. About 345 nm from the outer
surface the cement layer narrows abruptly to about 10 nm. The cement is
exposed on the inner surface of the shell, but
in most eggs a layer of shell about 10 nm thick covers the narrow outer
region of the cement layer. When experimentally
perforated eggs were incubated with trypsin, the wide inner layer of cement
was digested, but the narrow outer region
initially remained intact. These observations are discussed in relation
to the following (1) survival of the eggs during
embryonic development, (2) hatching, (3) the ‘hinge’ often
connecting the operculum to the empty egg-shell, (4) the
rapid hatching that occurs in some other monogeneans.