Field experiments were conducted in 2001 and 2002 to evaluate the efficacy of herbicides on protoporphyrinogen oxidase (protox, EC 1.3.3.4) inhibitor–resistant common waterhemp in corn and soybean. All corn herbicides tested gave greater than 90% common waterhemp control by 8 wk after postemergence herbicide treatment (WAPT). In soybean, common waterhemp control was less than 40% by 8 WAPT with postemergence protox-inhibiting herbicides lactofen and acifluorfen. However, preemergence protox-inhibiting herbicides sulfentrazone and flumioxazin gave greater than 85% common waterhemp control in both years. The greatest common waterhemp control in soybean was with glyphosate alone, alachlor + metribuzin, alachlor followed by (fb) glyphosate, and S-metolachlor + metribuzin fb glyphosate.

Field studies were conducted in 1995 and 1996 to investigate postemergence (POST) applications of rimsulfuron (12 g ai/ha) plus thifensulfuron-methyl (6 g ai/ha) in tank-mixtures with various acetolactate synthase (ALS)- and non–ALS-inhibitor herbicides for weed control in corn. Rimsulfuron plus thifensulfuron-methyl controlled giant foxtail and common lambsquarters at least 95% but did not control common ragweed. Rimsulfuron plus thifensulfuron-methyl tank-mixed with 20 g ai/ha primisulfuron-methyl, 17 g ai/ha CGA-152005 plus 18 g ai/ha primisulfuron, 18 or 36 g ai/ha halosulfuron-methyl, 18 g ai/ha nicosulfuron, or 280 g ai/ha dicamba controlled giant foxtail at least 89%, common lambsquarters at least 96% and, with the exception of the nicosulfuron combination, controlled common ragweed at least 88%. Rimsulfuron plus thifensulfuron-methyl tank-mixed with flumetsulam (26 g ai/ha) plus clopyralid (69 g ai/ha) plus 2,4-D (140 g ai/ha), atrazine (560 g ai/ha), 2,4-D (280 g/ha), or dicamba (308 g/ha) plus atrazine (588 g/ha) reduced the control of giant foxtail to less than 78% 26 d after treatment (DAT). Corn injury was less than 12% from rimsulfuron plus thifensulfuron-methyl and from mixtures of rimsulfuron plus thifensulfuron-methyl with other herbicides except when rimsulfuron plus thifensulfuron-methyl was mixed with flumetsulam plus clopyralid plus 2,4-D. This combination injured corn 26%. In these studies the appropriate tank-mix partners for rimsulfuron plus thifensulfuron-methyl were primisulfuron, CGA-152005 plus primisulfuron, and halosufluron-methyl.

Biotypes of eastern black nightshade were found in Illinois and Indiana that were not controlled by postemergence applications of imazethapyr. Greenhouse studies were conducted to determine whole-plant responses to the imidazolinone herbicides imazethapyr and imazamox and to the sulfonylurea herbicide primisulfuron-methyl. The Illinois and Indiana resistant biotypes were highly resistant to imazamox and imazethapyr but were not cross-resistant to primisulfuron-methyl. DNA sequencing of acetolactate synthase (ALS) genes showed that the molecular basis for resistance in both resistant biotypes was a single base-pair mutation within Domain C that changed an alanine to a threonine in their encoded enzymes. In vitro enzyme assays showed that the Illinois resistant biotype's ALS enzyme was 789- and 881-fold less sensitive than that of the susceptible biotype to imazamox and imazethapyr, respectively. The Indiana resistant biotype's ALS enzyme was 110- and 158-fold less sensitive than that of the susceptible biotype to imazamox and imazethapyr, respectively. These results are consistent with the amino acid substitution found in the ALS enzyme of both resistant biotypes of eastern black nightshade.

Palmer amaranth is a difficult-to-control broadleaf weed that infests corn and soybean fields in south-central and southwestern Nebraska and several other states in the United States. The objectives of this research were to confirm triazine and 4-hydroxyphenylpyruvate dioxygenase (HPPD)-inhibiting herbicide-resistant Palmer amaranth in Nebraska and to determine sensitivity and efficacy of POST-applied corn herbicides for control of resistant and susceptible Palmer amaranth biotypes. Seeds from a putative HPPD-resistant Palmer amaranth biotype from Fillmore County, NE were collected from a seed corn production field in fall 2010. The response of Palmer amaranth biotypes to 12 rates (0 to 12×) of mesotrione, tembotrione, topramezone, and atrazine was evaluated in a dose–response bioassay in a greenhouse. On the basis of the values at the 90% effective dose (ED90) level, the analysis showed a 4- to 23-fold resistance depending upon the type of HPPD-inhibiting herbicide being investigated and susceptible biotype used for comparison. This biotype also had a 9- to 14-fold level of resistance to atrazine applied POST. Results of a POST-applied herbicide efficacy study suggested a synergistic interaction between atrazine and HPPD-inhibiting herbicides that resulted in > 90% control of all Palmer amaranth biotypes. The resistant biotype had a reduced sensitivity to acetolactate synthase inhibiting herbicides (halosulfuron and primisulfuron), a photosystem-II inhibitor (bromoxynil), and a protoporphyrinogen oxidase inhibitor (fluthiacet-methyl). Palmer amaranth biotypes were effectively controlled (≥ 90%) with glyphosate, glufosinate, and dicamba, whereas 2,4-D ester provided 81 to 83% control of the resistant biotype and > 90% control of both susceptible biotypes.