Methiozolin selectively controls annual bluegrass in cool-season turfgrasses, and practitioners may wish to reseed desirable species in treated areas. Field experiments were conducted to evaluate reseeding intervals for creeping bentgrass, perennial ryegrass, and tall fescue following methiozolin applications. Turfgrass establishment varied for species, application timing (0, 2, 4, or 6 wk before seeding, WBS), and rates tested (0.56, 1.12, or 2.24 kg ai ha−1). Reductions in turf cover suggest that seeding of creeping bentgrass, perennial ryegrass, and tall fescue should be delayed 2 wk after methiozolin treatments at 0.56 kg ha−1. However, reseeding should be delayed after methiozolin treatments at 1.12 kg ha−1 for approximately 4, 4, and 2 wk for creeping bentgrass, perennial ryegrass, and tall fescue, respectively. Similarly, establishment was reduced on all dates from the nontreated after 2.24 kg ha−1 was applied at 4 WBS, suggesting that reseeding should be delayed for at least 6 wk on all three species at the high rate.