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1 results

  • A triazole linkage that mimics the DNA phosphodiester group in living systems

  • Afaf H. El-Sagheer, Tom Brown
  • Journal:
    Quarterly Reviews of Biophysics / Volume 48 / Issue 4 / November 2015
    Published online by Cambridge University Press:
    16 July 2015, pp. 429-436
    • Article
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  • We describe the development of a chemical process based on the CuAAC reaction (click chemistry) to ligate DNA strands and produce an unnatural triazole backbone linkage. The chemical reaction is templated by a complementary DNA splint which accelerates the reaction and provides the required specificity. The resultant 1,4-triazole linkage is read through by DNA and RNA polymerases and is biocompatible in bacterial and human cells. This work has implications for the synthesis of chemically modified genes and other large modified DNA and RNA constructs.