Invasive alien plants (IAPs) are a significant threat to the biodiversity and ecological functioning of the sub-Antarctic Prince Edward Islands (PEIs); however, weed biological control has not yet been implemented as a management tool. The PEIs have had 23 alien plants recorded, of which several are considered beyond eradication and therefore could benefit from the release of safe and effective biocontrol agents. We used the South African Biological Control Target Selection (BCTS) system to provide a ranked list of the target species in order of priority for further biocontrol research. The highest-scoring species, and therefore those that should be considered first to be targeted, were Sagina procumbens and Cerastium fontanum. No biocontrol agents for IAPs have been released in any climate with as consistent low temperatures as in the PEIs, so the climatic compatibility of agents will be essential prior to any decision to release. However, with very few native species present on the PEIs, the programmes would involve limited host specificity testing, which would improve the feasibility and speed with which new biocontrol agents could be developed. Biocontrol may offer an environmentally safe and sustainable method of reducing the negative impacts of these priority species and thus warrants further investigation.

The Spurgia esulae gall midge was introduced in North Dakota in 1986 as a biocontrol agent for leafy spurge. Spurgia esulae causes stem tip galls thereby decreasing seed production and it has been most successful near wooded areas. However, a second control method is needed to reduce the leafy spurge infestation and prevent spread from roots. Imazethapyr, 2,4-D, or picloram application did reduce the number of S. esulae galls but not the number of larvae per gall. Long-term S. esulae population was not affected by herbicide application. The integration of herbicides with S. esulae would prevent leaf spurge spread from a wooded area and would reduce seed production within an area where herbicides generally cannot be used.

Levels of inter- and intrapopulation genetic variation were determined in five North American populations of leafy spurge using chloroplast DNA (cpDNA) RFLPs and RAPD markers. Thirteen plastome types were identified among 123 individuals collected from five geographically separated populations. Number of plastomes within a population ranged from one to seven, with four of the populations having a predominate type plus one or more rarer types. Some plastome types were shared by populations, but plastome distribution among populations was nonrandom. RAPD markers indicated greatest relatedness among individuals within a population. Relatedness among populations as established through RAPDs was greater for geographically closer populations; this relationship was not observed for cpDNA markers. Differences in the range of movement for pollen and seed may account for differences between results of the cpDNA and RAPD analyses. The high degree of genetic variability among North American leafy spurge suggests possible multiple introductions or a high degree of variability within leafy spurge populations in its native range.

Literature concerning interactions between herbicides and insects is reviewed. Herbicides can be toxic to insects or can affect them indirectly, such as by destroying food supplies, but they usually can be used with proper application timing without affecting insects adversely. Herbicides and biocontrol insects can interact either favorably or unfavorably depending on the life cycle stage of weed and insect, effect on predatory insects, changes in food quality, mobility of the biocontrol insect, and impact of herbicides on weed growth. Thus, interactions between a biocontrol insect and herbicide can be antagonistic, additive, complementary, or synergistic. Split-plot techniques to evaluate these interactions at an early stage of insect establishment are proposed to improve weed biocontrol.

Germination stimulants were tested for effectiveness on velvetleaf seed imbibition and germination and concurrent microbial attack of seed to deplete weed seed in soil. Several chemicals increased in vitro seed germination and decreased the numbers of hard, viable seed. The proportion of nonviable seed, 5% with Fusarium oxysporum (Schlech.), was enhanced to 40% by adding ethephon at 100 μg/ml. Fungal density on seedling roots and imbibed (nonviable seed) was increased by chemical treatment. Seedling emergence was reduced 15% when ethephon or carbofuran was applied to soil with the fungus. Shoot dry weight decreased and root infection increased with all treatments regardless of fungal inoculation. Butylate and carbofuran increased infection of imbibed seed in soil by F. oxysporum while ethephon and AC94377 increased infection by other soil fungi.

The plant pathogenic fungus Colletotrichum coccodes (Cc) and the plant growth regulator thidiazuron (TDZ) were evaluated in Maryland and Quebec for velvetleaf control in ‘Williams' and ‘Maple Arrow’ soybean. TDZ was applied at 0, 0.1, 0.2, 0.3, and 0.4 kg ai/ha alone or was combined with Cc at 109 spores/m2 when velvetleaf was at the 1- to 2-leaf (Trial 1) or 4- to 6-leaf (Trial 2) growth stages. Velvetleaf control increased with TDZ rate, and TDZ combined with Cc further increased control. TDZ reduced velvetleaf biomass and height, and Cc increased velvetleaf mortality. In Quebec, Cc also reduced the biomass of velvetleaf treated in Trial 1 and interacted positively with TDZ at this growth stage. Cc nearly halved the rates of TDZ required for 90 and 75% mortality of velvetleaf treated at the 1- to 2-leaf and 4- to 6-leaf stages to 0.09 and 0.12 kg/ha, respectively, in Quebec. Cc similarly lowered the rate of TDZ required for 75% stand reduction of velvetleaf in Trial 1 to 0.17 kg/ha in Maryland. Cool wet weather in Quebec contrasted with warm, dry weather in Maryland. Soybean biomass and yield were increased significantly by treatment with TDZ plus Cc in Trial 1 at both locations.

The effects of the fungal protein Nep1 and Pseudomonas syringae pv. tagetis (Pst) applied separately or in combination on Canada thistle, common ragweed, and common dandelion were examined in growth chamber experiments. Experiments examined five treatments: (1) untreated control, (2) Silwet L-77 (0.3%, v/v) control, (3) Nep1 (5 μg ml−1) plus Silwet L-77 (0.3%, v/v), (4) Pst (109 colony-forming units [cfu] ml−1) plus Silwet L-77 (0.3%, v/v), and (5) Pst (109 cfu ml−1) and Nep1 (5 μg ml−1) plus Silwet L-77 (0.3%, v/v). Foliar treatments were applied at 28, 26, and 21 d after planting for Canada thistle, common dandelion, and common ragweed, respectively. For all three species, foliar application of Nep1 alone or in combination with Pst caused rapid desiccation and necrosis of leaves, with the greatest effect on recent, fully expanded (RFE) leaves. Within 4 to 8 h after treatment (HAT), 60 to 80% of RFE leaves of all three species were necrotic. Measured 72 HAT, Pst populations in Canada thistle leaves treated with Nep1 plus Pst were approximately 105 cfu cm−2 compared with 107 cfu cm−2 for leaves treated with Pst alone. Measured 2 wk after treatment, foliar application of Nep1 reduced shoot dry weight of the three weeds by 30 to 41%. Treatment with Pst reduced shoot growth of common ragweed, Canada thistle, and common dandelion by 82, 31, and 41%, respectively. The large suppression of common ragweed shoot growth caused by Pst treatment was associated with a high percentage (60%) of leaf area exhibiting chlorosis. Treatment with Pst plus Nep1 did not result in significant decreases in shoot dry weight for Canada thistle and common dandelion compared with either treatment alone. For common ragweed, shoot growth reduction caused by applying Pst and Nep1 together was not greater than that caused by Pst alone.

Dactylopius tomentosus is composed of biotypes adapted to different Cylindropuntia species. One biotype is an important biological control agent of C. imbricata in South Africa while another has the potential for the control of C. fulgida var. fulgida. These two weed species occur in sympatry in some areas of South Africa, so the introduction of the second biotype could result in hybridization, which, in turn, could impact on the biological control programs through altered host specificity and fitness of the hybrids. To anticipate what might happen, reciprocal crosses were made between the two biotypes, and the biological performance of the resultant hybrids was compared with that of each parental lineage on C. imbricata and C. f. var. fulgida. The biotypes interbred freely and reciprocally in the laboratory. Comparisons of crawler and adult female traits showed differences in performance that were dependent on the origin of the maternal and paternal genomes. However, when all traits were combined into a ‘fitness index’, both hybrids clearly outperformed the parental lineages. The increase in fitness shown by the hybrids over their maternal lineage was greater on the alternative host of the maternal parent than on the natural host of the maternal parent. Therefore, in areas where the two cacti occur in sympatry, hybridization between the biotypes is not expected to be detrimental to the biological control of either weed.

In a variety of ecosystems, interactions between soil microbiota and weedy plants can strongly affect population and community dynamics of these plants. However, weed–soil microbe interactions are not well characterized in field-crop agroecosystems. In Minnesota (USA), we repeatedly applied a fungicide (benomyl) to field plots in a corn–soybean crop rotation in each of 3 years, and sowed experimental weed communities containing host species for arbuscular-mycorrhizal fungi (AMF) and non-host species. Benomyl typically suppresses formation of mycorrhizal symbiosis in AMF-host plant species, and may also affect other soil fungi. We assessed weed density and biomass production, and monitored AMF colonization rates in each of 3 years. We found that weed density, biomass, community composition and the relative performance of AMF-host and non-host weed species were all significantly responsive to fungicide applications, although for all attributes responsiveness was variable. Fungicide application increased total weed density and biomass production in nearly all cases; most effects were modest but reached a maximum of 49%. Fungicide application also increased the relative performance of non-host species in most cases, although most effects were again modest. Our findings are the first assessment of responses by field-crop weeds to direct manipulation of soil microbial communities in a field setting, and suggest that the population and community ecology of these weeds can be strongly affected by the fungal component of soil microbiota.

Arbuscular mycorrhizal fungi (AMF) are plant root symbionts that provide many benefits to crop production and agro-ecosystem function; therefore, management of AMF is increasingly seen as important to ecological farming. Agronomic weeds that form a symbiotic relationship with AMF can increase diversity and abundance of agronomically beneficial AMF taxa. Also, AMF can strongly affect plant community composition, and may thus provide some degree of biological control for weeds. Therefore, relationships between weeds and AMF have a dual significance in ecological farming, but are relatively unexamined. In glasshouse experiments, seedlings of 14 agronomic weed species were grown in the presence or absence of AMF inocula sampled from each of three types of cropping systems: organic, transitional-organic or high-input/conventional. For each weed species, AMF root colonization rates and growth responses to AMF were assessed. On the basis of observed colonization levels, the species were classified as strong hosts (five species), weak hosts (three) and non-host species (six). Among species, biomass responses to AMF were highly variable. Strong hosts showed more positive responses to AMF than weak hosts, although the range of responses was great. Non-hosts did not suffer consistent negative biomass responses to AMF, although strong biomass reductions were noted for certain species–inoculum combinations. Biomass responses to inocula from different cropping systems varied significantly among weed species in one of two experiments. Results suggest that weed–AMF interactions can affect weed community dynamics. We recommend investigation of these interactions in agro-ecosystems that use management methods likely to intensify weed–AMF interactions, such as conservation tillage and cover cropping.

The main effects of gall midges in tropical agriculture are as primary pests of cultivated plants; as predators on pests, especially mealybugs, scale insects and mites; as potential biocontrol agents of weeds and as pollinators of cocoa. Published information is briefly reviewed under these main headings, with particular emphasis on the sorghum midge, Contarinia sorghicola (Coquillett), on the Asian and African rice gall midges, Orseolia oryzae (Wood-Mason) and O. oryzivora Harris & Gagné and on cecidomyiid predators on mealybugs and scale insects.