A simple model was derived relating the phenotypic effect (g) of a major gene to observed values of the population mean and variance for a trait, at specified values of the major gene frequency and at specified basal values of the population mean and variance (in the absence of the major gene). This model was applied to a total of 549 observed values of ovulation rate in ewes of the Cambridge breed at Bangor under a range of assumptions. The mean values of ovulation rate were 2·44 for 243 ewes of 1 year of age and 37·54 for 306 ewes of 2 and 3 years of age with a coefficient of variation for both age sets of 0·50.

The results indicate a minimum value for g, in this data set, of 1·07 for 1 year old and 1·72 for 2 and 3 year old ewes. The results are also consistent with a frequency value in the region of 0·3 to 0·4, with the absence of dominance and with a reasonable concordance with Hardy-Weinburg equilibrium. The results also indicate that the value of g varies according to the background phenotype since it is lower for younger as compared with older ewes.

The hypothesis that increasing the amount of straw offered and allowing animals to refuse proportionately more than 0·1 to 0·2 would increase intake and quality of the straw consumed was tested in two experiments with castrated goats (aged 6 to 31 months) and one with wether sheep (aged 6 to 18 months). Each trial (over 21 to 42 days following 14 to 35 days preliminary feeding) involved individually feeding long barley straw and concentrate supplement (15 g dry matter (DM) per kg live weight (M075) daily) and monitoring the quantity and quality of straw offered and straw refused. The results supported the hypothesis. In experiment 1, with 18 goats per treatment, those allowed to refuse 500 rather than 200 g straw per kg DM offered consumed more (18·9 and 14·4 g DM per kg M75 per day, s.e.d. 0·70) and their straw refusals contained more digestible organic matter (DOM) in vitro (347 and 320 g/kg DM, s.e.d. 7·7). For both treatments, refusals were less digestible in vitro than the straw offered (412 g DOM per kg DM). Thirty-six goats in experiment 2 (over 42 days) offered increasing amounts of straw (18, 54 and 90 g DM per kg M075 per day) consumed more (15·5, 22·8 and 26·2 g DM per kg M per day, s.e.d. 0·74), refused more (125, 566 and 703 g/kg DM offered) and the refusals were of increasing digestibility in vitro (354, 370 and 403 g DOM per kg DM, s.e.d. 14·5). All refused straw was inferior to that offered (443 g DOM per kg DM). The estimated intake of straw DOM was markedly improved by offering more straw (7·2, 12·8 and 14·5 g per kg M75 per day). Experiment 3, using 30 wethers over 21 days fed as in experiment 2, showed similar treatment responses, although absolute intakes of straw were lower. The responses observed are comparable to improvements in intake following treatment of straw with alkali. Further research is required to determine optimum feeding rates as affected by straw quality and animal productivity level. Practical feeding strategies will also need to consider utilizing refused straw.

Thirty-nine Hereford × Friesian autumn-calving cows (mean calving date 17 September) and their Charolais-cross calves were used in a 2 × 2 × 2 factorial experiment to assess the effects of feeding level in winter and sward height in summer on intake and performance. Mean estimated metabolizable energy intakes during winter (30 October to 6 May) were 63 (L) or 92 (H) MJ/day. Two sward heights, 4·8 (SI) or 7·6 (Tl) cm, were imposed from turn-out to weaning on 3 July, and a further two sward heights, 4·7 (S2) or 8·0 (T2) cm, from weaning to housing on 1 October. At turn-out the H cows were 17 kg heavier (P > 0·05) than the L cows and their calves were 30 kg heavier (P < 0·01). Cow herbage intakes were significantly higher on the Tl and T2 treatments compared with the SI and S2 treatments as were live-weight changes: 0·94 v. –0·01 kg/day (P < 0·001) for Tl and SI treatments and 0·96 v. –0·18 kg/day (P < 0·001) for T2 and S2 treatments respectively. Calves on the Tl treatment had higher live-weight gains than those on the SI treatment (1·40 v. 0·63 kg/day; P < 0·001). After weaning the SI cows had significantly higher herbage organic matter intakes than the Tl cows (P < 0·05) and had significantly higher live-weight gains (0·51 v. 0·28 kg/day; P < 0·05). Calving difficulty and calf birth weight were not affected by any of the treatments. The results demonstrate that both previous and current level of nutrition have an effect on cow performance. When cows are turned out to pasture at body condition scores of 2·25 to 2·5, the sward heights recommended for spring-calving cows are equally appropriate for autumn-calving cows.

Four silages were prepared from perennial ryegrass: two unwilted, ensiled either without (UWN) or with formic acid additive (UWA) and two wilted for either 19 (WS) or 43 h (WL) prior to ensiling. The influence of conservation method on digestion in the rumen and the flow of organic mater (OM) and nitrogen (N) to the small intestine was studied using young steers fitted with rumen and duodenal T-piece cannulae. The silages were well preserved and of similar chemical composition despite a 1·5-fold increase in dry matter (DM) following wilting. The unwilted silages had a higher concentration of fermentation acids and a lower concentration of soluble N in the DM. Polyester bag studies showed that significantly more DM was potentially degradable but the degradation rate was slower in the rumen of steers fed UWA and WS silages (P < 0·05), however, rate of passage was not significantly different for the four treatments. Conservation method had little effect on post-feeding rumen ammonia concentration but feeding unwilted silages caused a greater depression in post-feeding rumen pH (P < 0·05). Wilting lowered N digestibility in the whole tract (P < 0·05), but had no effect on apparent digestion or degradability of N in the rumen as measured by the polyester bag method. Apparent OM digestibility in the whole tract was lower in steers given wilted rather than unwilted silages (P < 0·05), but disappearance of apparently digestible OM in the rumen was greatest for the 19 h wilted silage (WS, P < 0·05). It is concluded that, provided unwilted silage is well preserved, the use of formic acid has little effect on digestion of silage by steers and the effects of wilting are confined to a reduction in whole tract digestibility.

The effect of body condition on hypothalamic and pituitary function in post-partum suckled beef cows was studied. Thirty-eight Blue-Grey cows were calved at a body condition score of either 2·35 (L) or 2·80 (H), fed to maintain live weight and body condition thereafter and ovariectomized at either 5 or 9 weeks post partum. The H cows had higher LH pulse frequencies before ovariectomy (0·36 v. 0·28 pulses per h; P < 0·05) and 15 days after ovariectomy (0·86 v. 0·72 pulses per h; P < 0·01), indicating a direct effect of body condition on GnRH release from the hypothalamus. Body condition had no effect on the ability of the pituitary to release LH in response to a physiological dose (2·5 u.g) of GnRH in entire or ovariectomized cows at 5 or 9 weeks post partum. The magnitude of the increase in circulating levels of LH following GnRH injection was greater at 9 weeks compared with 5 weeks post partum (2·23 v. 1·73 u.g/1; P < 0·001) in entire cows, but there was no difference between 5 and 9 weeks in ovariectomized cows. Following ovariectomy there was a 2·5-fold increase in LH pulse frequency within 15 days and LH pulse frequency approached 1 pulse per h in all treatments groups. It is concluded that both the hypothalamus and the pituitary are capable of functioning normally by 5 weeks post partum in suckled beef cows, that the pulsatile release of GnRH from the hypothalamus is inhibited, possibly due to a very high sensitivity to oestradiol, and that the sensitivity of the pituitary to the negative feedback effect of oestradiol may decrease with time post partum.

A crossmothering experiment was conducted to measure the direct and the maternal components of the response to divergent selection for yearling growth rate in beef cattle. The animals were from three closed lines of Angus cattle. Two lines had been selected since 1974 for either high (high-line) or low (low-line) average daily gain from birth to yearling, and the third line was maintained as a randomly bred control-line. A total of 221 female calves born between 1984 and 1987 was used in the crossmothering experiment, and an additional 113 cows bearing calves in 1988 were used to obtain more records of milk production.

On average, high-line calves born in 1984-87 were 45 kg heavier at weaning (200 days of age) than low-line calves and 65 kg heavier at yearling age, corresponding to a proportional divergence in daily weight gain of 0·29 and 0·32 respectively. The direct component of the response to selection was 0·82 (s.e. 0-05) of the divergence in body weight at weaning and 0·89 (s.e. 0·05) at yearling age. The maternal component was 0·18 (s.e. 0·06) and 0·11 (s.e. 0·04) for weaning and yearling weight respectively. Over the years 1984-88, high-line dams produced 1·15 times the milk of low-line dams and only 1·03 times that of control-line dams. There were small differences in the composition of milk sampled in 1984 which resulted in the milk of high-line dams having a higher content of metabolizable energy (ME) than that of control-line dams. The ME in the milk consumed by the calves from the three selection lines was sufficient to fuel similar proportions of their pre-weaning growth and indicated that the expression of the maternal component of the selection response may be via small differences in the quantity and quality of the milk produced by the dams. Expression of the direct component appeared to be at least partially via differences in appetite of the calves. These results, together with results for sheep, mice and rats, show that the direct component of the response to selection for growth is much larger than the maternal component.

Two experiments were conducted to investigate the basis for an increase in ovulation rate in ewes given improved nutrition prior to mating.

Sixteen wether sheep were used in the first experiment in order to screen physiological and nutritional variables likely to be affected by protein and energy intake. They were given low protein (LP) and high protein (HP) diets (19·1 and 35·2 g nitrogen per kg dry matter (DM)) each at four levels of energy intake (range 7·67 to 27·06 MJ gross energy per day). Effects of treatment on digestibility and partition of digestion between the rumen and intestines were small but increased nitrogen intake increased both nitrogen retention and the concentration of some essential amino acids (EAA) in plasma. The dietary treatments imposed on the wethers had been given to 800 ewes previously in a separate trial designed to predict ovulatory response to dietary and energy intakes. When the variables measured in the wether sheep were compared with predicted ovulation for ewes given the same treatments, the strongest relationship was with plasma concentrations of branched-chain amino acids (BCAA; r = 0·95; P < 0·001). Correlations with other EAA were weaker (r = 0·61; P < 001) and were not significantly related to concentrations of non-essential amino acids (NEAA) in plasma (r = −0·21).

Eighty ewes in experiment 2 were given an LP diet, blood samples were taken and ovulation rate determined by laparoscopy. One group of 20 ewes was maintained on the diet and another group of 20 was given an HP diet starting on day 7 of the oestrous cycle. Blood samples were taken 5 days later and numbers of ova determined during the next cycle. There were no changes in the number of ova present in ewes maintained on the LP diet, but a change to the HP diet increased ovulation rate from 1·47 to 1·89 eggs per ewe. Ewes which had single or multiple ova when given the LP diet (pre-treatment) had similar concentrations of amino acids in their blood, but those given the HP diet increased blood concentrations of BCAA by proportionately 0·58 and other EAA by 0·29 whilst NEAA were unaffected. Ewes which increased their ovulation rate when given the HP diet had a greater increase in blood BCAA concentration than those which did not increase their ovulation rate. Both the concentration in blood and the magnitude of increase in BCAA concentration appear to affect the number of eggs released. This concept is supported by evidence that BCAA are able to exert regulatory effects on mammalian protein metabolism under some circumstances.

The effect of body condition at calving on ovarian follicle characteristics during post-partum anoestrus was studied in 38 suckling Blue-Grey cows with mean body-condition scores of 2·80 (s.e. 0·05) (H) or 2·35 (s.e. 0·05) (L) at calving and fed so that they maintained live weight and body condition during lactation. Cows of each level of body condition were ovariectomized at either 5 or 9 weeks after calving and follicles ≥3 mm in diameter were dissected out and incubated in a medium (Medium 199) for 2 h. Follicle incubates were assayed for progesterone, testosterone and oestradiol. L cows had fewer follicles ≥3 mm in diameter than H cows (24·1 v. 44·9; P < 0·05) at 5 weeks post partum due to a lower number of small (3 to 4 mm) follicles (12·9 v. 30·6; P < 0·05). At 9 weeks there was no effect of body condition on mean numbers of follicles (38·1 v. 40·4; P > 0·05). The proportions of healthy, intermediate and atretic follicles in all cows were 0·35, 0·20 and 0·45 respectively; these proportions were not affected by body condition or time of ovariectomy. Body condition did not affect the overall mean capacity of the cultured follicles to produce oestradiol or progesterone in vitro but testosterone production was higher in follicles from H than L cows (6406 v. 5206 pg/h; P < 0·05). However, at 9 weeks post partum a greater proportion of H than L cows had a large (>8 mm), healthy, highly oestrogenic follicle (4/8 v. 1/7; P = 0·18).

At 9 weeks post partum, the four large, healthy, oestrogen-active follicles (those secreting >250 pg/h) from H cows were approximately 11-fold more oestrogenic than the single comparable follicle from L cows (7100 v. 635 pg/h; P > 0·05). There was little difference at week 5 post partum: 3288 and 2785 pg/h (P > 0·05) for H and L cows respectively. Oestrogen-active follicles from H cows were significantly more oestrogenic at 9 than 5 weeks post partum (5807 v. 3021 pg/h; P < 0·05). The results suggest that the body condition-related reduction in the number and steroidogenic capacity of large oestrogenic follicles in post-partum cows may be associated with a reduction in LH pulsatility.

In three experiments, seven grass silages, four grass hays and winter barley straw, with and without anhydrous ammonia treatment, were offered ad libitum to a total of 134 Finn Dorset ewes in weeks 11 to 15 of pregnancy and intakes were measured. The untreated straw diet was supplemented with 300 g fresh weight of concentrates per ewe. Number of foetuses carried did not affect intakes in mid pregnancy. Organic matter digestibilities measured in vivo in week 15, ranged from 0·39 to 0·69 for hays and 0·53 to 0·79 for silages. Generally, between weeks 11 to 15 of pregnancy, hay intakes showed either little change or slight increases whereas intakes of silages or straws showed little change or slight decreases. Forage intake was higher on the better quality hays and silages and ewes gained weight and body condition, compared with losses for the poorer diets. In late pregnancy, the ewes were offered grass silage ad libitum and rations of concentrates according to a rationing programme. There were no residual effects of the mid-pregnancy treatments on lamb birth weights but ewes offered the poorer diets in mid pregnancy still tended to be lighter and thinner post partum. Equations are presented to predict the intakes of hays and silages from food and animal factors.

Rumen degradability of dry matter (DM), organic matter (OM), nitrogen (N), neutral-detergent fibre (NDF), hemicellulose, cellulose and lignin was evaluated with the in situ technique for maize silage and cocksfoot, timothy, fescue, lucerne and meadow hays. The degradability of each of the six forages was studied separately, each forage being used in turn as the main component of the diet offered to four fistulated cows. For each forage 300 g were mordanted with sodium dichromate and placed in the rumen when the same forage was studied. Faecal grab samples were collected to measure the forage transit time. Digestibility was evaluated using both lignin as an indicator and by an in vitro method.

Rumen outflow rate was higher for cocksfoot and lucerne hays than for maize silage and the meadow, timothy and fescue hays (P < 0·01). The effective degradabilities of DM and OM were higher in maize silage, fescue and lucerne than in cocksfoot, timothy or meadow hay (P < 0·01). Effective degradability of N was highest in lucerne and lowest in timothy and meadow hay (P < 0·01). The degradability of NDF, hemicellulose and cellulose for fescue was always the highest of the six forages (P < 0·01; P < 0·05; F < 0·01 respectively).

Rumen outflow rate was statistically correlated with the c value of DM (r = 0·47), N (r = 0·54), NDF (r = 0·43) and hemicellulose (r = 0·43). High correlations were observed between rate constants of degradation of NDF and hemicellulose, cellulose or lignin (0·93, 0·75 and 0·79 respectively). The regression between in vitro and lignin-derived digestibility was highly significant (P < 0·001, r2 = 0·902 residual s.e. 0·017). The multiple regression analysis between lignin-based digestibility and degradability coefficients, effective degradability and coefficients of faecal chromium excretion was highly significant (r = 0·748; residual s.e. = 0·03).

Twenty-four Finn ♂ × Dorset Down ♀ wether lambs about 10 weeks and weighing on average 18 kg were allocated to treatment groups as follows: untreated controls (U); 15 mg oestradiol-17β (OE) implanted subcutaneously (O); cimaterol (2 mg/kg dry matter (DM)) in the diet (C); and 15 mg OE implant + cimaterol (2 mg/kg DM in the diet) (OC). The lambs were offered a diet, restricted to 38 g/kg live weight (LW), and which contained an estimated 12·5 MJ metabolizable energy and 137 g crude protein per kg DM for the experimental period of 62 or 63 days. Comparisons were made for the main effects of OE and cimaterol and also interactions between OE and cimaterol. Treatment with OE, on average, resulted in greater LW gain, DM intake, empty body weight (EBW), body length and chest depth, weight of shoulder and loin joints and carcass crude protein, but had no effect on fat deposition in carcass or depots. As a proportion of EBW, the weights of the pituitary gland and accessory vesicular glands were increased, as was teat length in lambs treated with OE. Cimaterol treatment, on average, increased LW gain, EBW, killing-out ratio, carcass weight and its weight of crude protein and, unlike OE, decreased the weight of the pituitary gland as a proportion of EBW and reduced the weight and proportion of fat in the carcass and perirenal and retroperitoneal depots. Increases in the cross-sectional area of m. longissimus dorsi and in the weight of selected major and minor commercial joints were also obtained in cimaterol-treated lambs. The presence of significant interactions indicated that there was incomplete or no additivity between the effects of cimaterol and OE for LW gain, cross-sectional area of m. longissimus dorsi and weights of carcass and carcass crude protein, omental fat and of certain of the dissected joints. These results suggest either that the mechanisms of action of oestradiol-17β and cimaterol are not independent or that they are affected by similar metabolic or physiological processes which limit the ability of castrated male sheep to exhibit a positive response.

Five diets of identical metabolizable energy (ME) content were formulated, in which wheat silage, made from a variety bred for a high proportion of ears to roughage, was incorporated at the rate of 480 g/kg dry matter (DM). Their calculated ME density was 11-4 MJ/kg DM, the protein concentration of four of the five diets was 150 g/kg DM, and that of the fifth was 87 g/kg DM. The effectiveness of protein supplementation of such diets by non-protein nitrogen (NPN), fish meal (FM) or soya-bean and cottonseed oil meals (SBM and CSM) was studied in a digestibility and nitrogen balance trial, and in a feeding trial using Friesian and beef breed young bulls. One diet served as a negative control (NC). The apparent digestibility of organic matter (kg/kg DM) was 0·709, 0·708, 0·704, 0·680 and 0·667 (s.e. 0·0125) and nitrogen retention (g/kg M0·75 per day) was 0·20, 0·65, 0·65, 0·64 and 0·68 (s.e. 0·059; P < 0·01) for the NC, NPN, FM, CSM and SBM supplemented diets, respectively. The feeding trial was divided into two periods; the first 100 days, and from day 101 to slaughter. The NC groups of both breeds gained significantly less in both periods of the experiment than did the supplemented groups. There were no significant differences among the supplemented groups.

Three groups of lambs of about 25 kg live weight were given a concentrate diet either on its own (eight lambs) or supplemented with 10 g/kg ammonium chloride (eight lambs) or 20 g/kg sodium bicarbonate (12 lambs). At about 45 kg live weight the lambs were killed and their body composition was determined. The composition of their gains was also determined using information obtained from a fourth group of lambs (eight lambs) which were killed at the start of the experiment. Lambs given the ammonium chloride diet showed a reduction in blood pH while those given the sodium bicarbonate diet showed an increase to levels seen in lambs given forage diets. There were also marked effects on calcium (Ca), phosphorus (P) and magnesium retention with retentions being reduced in those given the ammonium chloride diet and increased in those given the bicarbonate diet. The average rates of retention of Ca and P in lambs given the bicarbonate diet were 9·1 and 5·9 g/kg empty-body weight gain and are comparable with rates seen in lambs given forage diets. These results suggest that dietary induced changes in blood acid-base status is a major factor contributing to the lower rates of retentions of these minerals in lambs given cereal-based diets.

Growing heifers (experiment 1) and wether lambs (experiments 2 and 3) were offered ammoniated cereal straw with or without a protein meal. The effects of supplementation with long-chain fatty acids (LCFA; as calcium salts of LCFA (CaLCFA) or fat prills) on nutrient utilization were studied.

Intake of the basal diet (g/kg live weight) was unaffected by the protein meal, but was often reduced by supplementation with LCFA, especially fat prills. Live-weight gain was increased both by supplementation with protein meal and CaLCFA. Fat prills only increased live-weight gain in the presence of protein meal and depressed live-weight gain in the absence of protein meal. There were small differences between the two sources of LCFA in their apparent effects on rumen fermentation.

Supplementation with protein meal increased relative protein content (P < 0·05) and tended to increase water content (P > 0·05) in the wethers in experiment 3 (corrected to equal empty-body weight at slaughter). Of the LCFA, only CaLCFA tended to increase relative body fat content (by proportionately 0·23; (P > 0·05) but decreased relative protein and water content by 0·05 and 0·06 (P < 0·05).

Supplementation of straw-based diets with as little as 20 g CaLCFA per kg food dry matter improved live-weight gain and efficiency of nutrient utilization of ruminants, particularly when offered in combination with a protein meal. There appeared to be marked differences in the effects of CaLCFA and fat prills on food intake, productivity and to some extent body composition.

Dairy ewes (Prealpes breed) were subjected to differential photoperiod treatments: either long (15·5 light (L): 8·5 dark (D) or short (8·5 L: 15·5 D). Treatments started 15 days before lambing and continued until the 5th week of lactation. After lambing the 22 ewes of each photoperiodic treatment were divided in two groups and fed on either a high or low plane (19 v. 10·5 MJ/day and 370 v. 237 g crude protein per day). Milk yield was not affected by photoperiod but significantly modified by feeding levels (1154 v. 789 ml/day). Protein content of the milk was reduced under long days (—4·9 g/1) compared with short days, while no effect was observed for fat content. Body chemical composition changes, estimated by the deuterium oxide (heavy water) dilution technique between weeks 1 and 4, were affected by both treatments, with a significant interaction between photoperiod and diet for body protein changes: under short days lipid and protein changes were closely related while under long days they were independent.

Blood samples were taken on day 23 of lactation from six ewes per group, every 30 min from 07.30 to 15.30 h for growth hormone (GH) and prolactin (PRL) measurements. The timing of GH and PRL pulses were different, but there was evidence that secretory patterns of each hormone were synchronized across ewes within the four treatments. Long photoperiod increased plasma PRL baseline rather than pulse amplitude, with no effect of diet. Undernutrition mainly increased GH pulse amplitude rather than baseline concentration, with no significant effects of photoperiod.

High spontaneous concentrations of GH were associated with body lipid mobilization (R2 = 0·51) and were also involved in the control of protein changes (R2 = 0·71). These results indicate that GH (and PRL) are involved in nutrient partitioning in the lactating ewe.

A dynamic, mechanistic model of lamb metabolism and growth was developed for the purpose of evaluating hypotheses regarding the mechanisms of action of growth promotants. The model relates tissue growth to DNA accretion and protein turn-over. State variables include circulating amino acids, glucose, lipids and acetate; four protein pools (carcass, viscera, other tissues and wool) and storage triacylglycerol are also included. Equations are mainly of the Michaelis-Menten form, allowing for nutrient utilization patterns to be determined by relative tissue affinities for substrates (ko.5), enzymatic capacities (Vmax) and substrate concentrations ([S]). Protein degradation rates are defined as first-order with respect to protein. The model adequately simulated growth from 20 to 40 kg empty body weight. Simulated changes in nutrient input yielded reasonable energy balance response patterns, although theoretical growth efficiencies were greater than those observed in practice. Variations in volatile fatty acid absorption patterns were accommodated well, with predicted nitrogen retention closely approximating experimental observations. The model also responded appropriately to changes in dietary protein level, with body fat varying inversely with amino acid absorption. In summary, the model was found to perform adequately for the purpose of examining mechanisms responsible for alteration of growth and body composition.

Responses of lambs to cimaterol (CIM), diethylstylbestrol (DES) and ovine growth hormone (GH) were examined using a mechanistic model of growing lamb metabolism. All three compounds increase growth of lean tissue (protein) and decrease fat gain, although the magnitudes of these responses vary. Our working hypothesis was that observed changes in nutrient partition between lean and fat gain were caused by alteration of rate constants for turn-over of muscle protein and fat. Individual experiments were simulated whilst varying values of the protein degradation constant (Kprolein) and Vmax for lipolysis (Kfat). Optimal parameter values were found by minimizing residual errors, calculated as the deviations of model predictions from experimental values for carcass protein and fat. Fitted values of Kfat and Kprotein (expressed as proportions of controls) for each simulation were: CIM (grazing), 1·20 (s.d. 0·05) and 0·86 (s.d. 0·025); CIM (pellet-fed), 1·11 (s.d. 0·115) and 0·87 (s.d. 0·032); DES, 1·33 (s.d. 0·111) and 0·94 (s.d. 0·024); GH, 1·77 (s.d. 0·139) and 0·97 (s.d. 0·025) respectively. These results demonstrate that different mechanisms may be responsible for the changes in carcass composition due to 3-adrenergic agonists, anabolic steroids and growth hormone. CIM probably exerts its effects via changes in protein and fat metabolism, whereas DES and GH appear to act mainly through changes in adipose tissue, with little or no effect on the rate constant for protein turn-over. Carcass composition is less sensitive to manipulation of adipose tissue metabolism than to changes in muscle protein metabolism.

During the first 2 weeks after kidding, 27 multiparous British Saanen goats were offered 1·5 kg hay (98 g crude protein (CP) per kg dry matter (DM)) and from 0·45 increasing to 0·70 kg concentrates (152 g CP per kg DM) daily. Week 2 was used as a covariance period. At week 3 of lactation all the goats were allocated to one of three groups and were offered hay ad libitum and 1 kg concentrates containing 117 (LP), 152 (MP) or 185 (HP) g CP per kg DM daily to week 15. During weeks 4 to 15, hay intake and milk yield were highest in the HP group. With increasing protein in the concentrates, hay DM intake was 1·20, 1·19 and 1·37 (s.e. 0·060) kg/day respectively and milk yield was 3·04, 3·21 and 3·36 (s.e. 0·080) kg/day respectively (linear response P < 001). There was no significant effect on the concentration of solids-not-fat, fat, total nitrogen or casein in milk. The digestibility of dietary organic matter, acid-detergent fibre and total nitrogen measured after week 15 was significantly greater with HP than with LP (P < 0·05), with MP values being intermediate (linear response P < 005). The rate of passage of ytterbium-labelled hay was unaffected by the treatments. The results indicate that, with lactating goats given hay and concentrates, beneficial responses may be expected to increasing concentrate CP to at least 185 g CP per kg DM.

Forty Menz rams weighing 20 to 22 kg and aged 13 to 18 months were individually offered 1·0 kg/day chopped maize stover and one of five supplements over a 70-day growth trial followed by a 10-day metabolism trial. The amounts of supplement offered were designed to supply 4·4 g nitrogen per day and with stover, sufficient nutrients to allow growth rates of 50 g/day. The control supplement, 80 g dry matter (DM) per day extracted noug (Guizotia abyssinica) meal, was compared with 194 to 212 g DM per day of fruits (pods (carpels) with seeds) of Acacia tortilis, A. albida, A. nilotica and A. sieberiana. For the control and four Acacia supplements respectively, mean growth rates were 32, 32, 21, 16, 4 (s.e.6) g/day and mean stover intakes 483, 430, 401, 347, 320 (s.e. 74) g DM per day. The low growth rates and intakes with A. sieberiana and to a lesser extent A. nilotica were probably related to their content of phenolic compounds including insoluble proanthocyanidins which may have lowered the digestibility of neutral-detergent fibre and nitrogen. A. tortilis and A. albida would appear to be of comparable nutritive value to noug meal as supplements to maize stover. Unlike noug meal Acacia fruits are widely available in rural areas of Africa. Further research is required to identify other tree species suitable for use as supplements and how to overcome the anti-nutritional factors.

Two experiments were conducted to determine the effect of including sucrose in diets on energy utilization by growing pigs. In the first experiment, the digestible energy (DE) content of sucrose was determined as 15·6 MJ/kg, indicating that gross energy was 0·96 digested. In the second experiment, iso-energetic diets were used to determine the effects of sucrose on growth and nutrient utilization. Sucrose was used to replace wheat gradually in four iso-energetic diets (15 MJ DE, 0·75 g lysine per MJ DE for pigs from 20 to 50 kg live weight and 15·1 MJ DE, 0·67 g lysine per MJ DE for pigs from 50 to 80 kg live weight). Pigs were fed ad libitum. Food intake (P < 0·01) and carcass daily gain (P < 0·05) increased with sucrose inclusion, independently of the level of sucrose in the diet. Food conversion ratio of male (boar) pigs on a carcass basis was unaffected by sucrose inclusion but increased with female pigs (P < 0·01). Killing-out proportion increased linearly with sucrose inclusion (P < 0·01). Sucrose inclusion improved energy retention (P < 0·05) and increased fat deposition (P < 0·05) but protein deposition was not affected. The weight of the full viscera (P < 0·01), empty digestive tract (P < 0·05), stomach (P < 0·01) and large intestine (P < 0·05) decreased with increasing sucrose inclusion. Sucrose did not affect blood triglycerides or cholesterol concentration (P > 0·05). Sucrose inclusion decreased the crude fibre and crude protein content of the diet and energy utilization was improved. Differences in gut fill indicated that sucrose-based diets were rapidly digested and absorbed.