As a part of the multidisciplinary project entitled ‘Archaeological Investigations into the Late Pleistocene and Early Holocene of the Lim Channel, Istria’, archaeological research has been conducted at four sites: Romuald's Cave, Abri Kontija 002, Pećina at Rovinjsko Selo and Lim 001 (Figure 1). There is much debate on issues related to biological and behavioural continuity, to patterns of changes and adaptations during this crucial period, and to external factors (e.g. changes in ecology and climate). For example, a clearer insight is needed into how climatic change affects the ecology of specific regions, including changing sea levels. Additionally, there continues to be debate centring on who produced the earliest (Initial) Upper Palaeolithic industries in Europe. To achieve a more precise insight into long-term diachronic changes and cultural relations around the Adriatic, and to document the presence of Middle and Upper Palaeolithic humans in Istria, we concentrated on a single microregion (the Lim Channel in Istria, Croatia). Here we report work on the two sites that to date have yielded Pleistocene material: Romuald's Cave and Abri Kontija 002.