Genetically modified, herbicide-resistant crop (HRC) cultivars, which allow for simplified weed control decisions compared with conventional cultivars, have considerable potential in Latin America. The number of herbicide applications can be reduced in HRCs, and otherwise difficult-to-control species, including red rice in rice or herbicide-resistant weeds, can potentially be managed. The American tropics include the centers of origin of several crops, such as corn and potato, and natural or agrestal floras containing wild near relatives of introduced crops, including rice and cotton, for which HRCs could be used. Potential direct impacts of HRC adoption on biodiversity in Latin America include changes in the genetic diversity of crops, increased volunteer crop problems, and invasion by resistant cultivars of natural areas beyond the farm boundary. Additionally, there is a risk associated with the escape of transgenes from HRCs, involving introgression to weedy relatives on a field scale leading to amplification effects of existing weeds or modification of gene pools of crop progenitors in the centers of origin or diversity. Possible indirect effects of HRCs include the potential expansion of agriculture into uncleared wild areas made economically attractive by the more efficient cropping system, and adverse effects on nontarget organisms and ecosystem processes. The occurrence of spontaneous hybrids of crop–weed or near-relative complexes is known in the United States for rice and sunflower, and the development of HRC–weed hybrids would provide a particularly difficult set of weed management problems. For crops in which HRCs are currently in use or under development, the greatest risks in Latin America appear to be with corn, cotton, and potato. However, some of the genetic and geographical barriers reduce the risk of hybridization between these crops and their wild relatives. Furthermore, unlike the case of feral rapeseed on road verges in Europe, there is no known example of a conventionally bred crop or interfertile hybrid with a near relative becoming established outside of cultivation in Latin America. Soybean, the most widely adopted HRC in Latin America to date, is an exotic beyond the range of its wild relatives. With the exception of O. glumaepatula, there appears to be little threat to endemic wild species from HRC rice as strong infertility barriers should prevent transgene flow, but possible transgene movement to the conspecific red rice may become an issue. Beyond the field boundary, a key question concerns the possible persistence of HRC x wild relative hybrids and the manner in which resistance traits affect their overall fitness. Designing practical risk assessment protocols on which to base HRC release approval is a considerable challenge, and it is vital that Latin American states continue to build and maintain functional biosafety regulatory structures.

Field experiments were conducted at five sites in Nebraska in 2000 and 2001 to determine the effect of planting depth and isoxaflutole rate on the response of an isoxaflutole-sensitive corn hybrid, ‘Pioneer 33-G’ across variable environments. Corn was planted at depths of 2.5 and 5.0 cm, and isoxaflutole was applied PRE at the recommended (1×) and twice the recommended (2×) rate. The effects of planting depth and herbicide rate on injury varied considerably across site–years. When injury was evident, it was generally greater at the high rate of isoxaflutole (2×) and at the shallow planting depth (2.5 cm). In most site–years, corn recovered from early season injury, and yields were not reduced, except at Scottsbluff, NE, and North Platte, NE, where soils were lower in organic matter and higher in pH. Isoxaflutole rates should be carefully selected for soils with low organic matter and high pH.

Foxtail, foxtail, where have you been? Been down to China, and I come back again! Foxtail, foxtail, where are you goin'? Any old where that the wind is a' blowin'!

James A. Duke, 1992.

Experimental controlled release starch granules (CRSG) containing 5.3% a.i. (w/w) norflurazon or 6% a.i. (w/w) simazine retarded the leaching of both herbicides in loamy sand soil columns when compared to commercial formulations of norflurazon [80% (w/w) dry flowable] or simazine [90% (w/w) water dispersible granule]. Barley bioassays indicated norflurazon and simazine remained in the surface 0 to 2.5 cm of soil when applied as CRSG formulations and moved to a depth of 15 cm when applied as commercial dry formulations and leached with 6 cm of water. CRSG placed on pre-wetted soil columns began to release norflurazon by 7 d at 25 C or 14 d at 15 C, and subsequent leaching moved norflurazon beyond the top 2.5 cm of the soil column.

Controlled-release formulations have the potential to reduce atrazine leaching but may reduce herbicide bioavailability and weed control. This research was conducted to determine if starch-encapsulated atrazine produced by the extrusion process could be modified to provide an optimum rate of herbicide release for adequate weed control and reduced leaching potential in sandy soils. The dry flowable (DF) and six starch-encapsulated atrazine formulations of various mesh size (14 to 20 and 20 to 40), clay content (0 and 5%), and extruder processing speed (200 and 400 rpm) were evaluated. All starch encapsulations reduced the amount of atrazine leaching from the surface 5 cm of soil columns by an average of 68% compared with the dry flowable formulation. Compared with the larger starch granules (14 to 20 mesh) 8% more atrazine in the smaller granules leached from the surface 5 cm. Atrazine efficacy was measured using an oat bioassay conducted in the greenhouse. Smaller granules (20 to 40 mesh) provided equivalent efficacy to DF while large granules (14 to 20 mesh) did not. Extruder speed and clay content did not affect efficacy or leaching potential. This study indicates that finer starch granules should result in faster atrazine release and improved weed control while still reducing the leaching potential of atrazine.

Field studies were conducted near Crowley, LA, in 2005 through 2007 to evaluate the effects of simulated herbicide drift on ‘Cocodrie’ rice. Each application was made with the spray volume varying proportionally to herbicide dosage based on a constant spray volume of 234 L ha−1 and an imazethapyr rate of 70 g ai ha−1. The 6.3%, 4.4 g ha−1, herbicide rate was applied at a spray volume of 15 L ha−1 and the 12.5%, 8.7 g ha−1, herbicide rate was applied at a spray volume of 29 L ha−1. An application of imazethapyr at one-tiller, panicle differentiation (PD), and boot resulted in increased crop injury compared with the nontreated rice. The most injury observed occurred on rice treated at the one-tiller timing. Imazethapyr at one-tiller, PD, and boot reduced plant height at harvest and primary and total (primary plus ratoon) crop yield, with the greatest reduction in primary crop yield resulting from imazethapyr applied at boot. Imazethapyr did not affect rice treated at primary crop maturity.

Smooth barley has emerged as a problematic weed in cereal crops of South Australia. After the recent reports of herbicide resistance and increase in seed dormancy in smooth barley, it was considered important to determine the herbicide resistance status and seedbank behavior of field populations of this weed species. A field survey was undertaken in the Upper North and Eyre Peninsula regions of South Australia in October 2012. Of the 90 smooth barley populations screened for resistance to quizalofop, 15% exhibited some level of resistance and 85% were susceptible. Resistance to acetolactate synthase (ALS)-inhibiting herbicides was low, with only 3 and 12% of populations classified as developing resistance to imazamox + imazapyr and sulfosulfuron, respectively. No multiple resistance patterns were observed; however, two ALS-inhibiting herbicide-resistant populations had sulfonylurea-to-imidazolinone cross-resistance. At the start of the growing season, the majority of smooth barley populations emerged rapidly (median 50% time to emergence [T50] = 8 d). In contrast, some populations of smooth barley displayed an extremely slow emergence pattern, with T50 of > 20 d. No direct linkage between seed dormancy and herbicide resistance was observed. However, two acetyl coenzyme A carboxylase-inhibiting herbicide-resistant populations were highly dormant and exhibited delayed emergence. The majority of smooth barley populations showed low-level or no seedbank persistence, but a few populations persisted for 1 yr. However, some weed populations had up to 20% seedbank persistence from 1 yr to the next. Overall there was a strong negative relationship between smooth barley seedling emergence and the level of seed persistence (R2 = 0.84, P < 0.05). This association indicated that greater seed dormancy could be responsible for extended persistence of the seedbank of this weed species. The study provides valuable insights into the general pattern of herbicide resistance and the behavior of the seedbank of smooth barley populations on South Australian farms.

Slow carrot emergence and canopy development render the crop a poor competitor with weeds. In this study, the ability to suppress weeds and maintain yield in the presence of weeds was compared among nine carrot varieties that included those selected by plant breeders for rapid vegetative canopy development compared to traditional varieties. Two weed management treatments were compared: handweeding for 21 d after carrot seeding versus handweeding for the entire carrot season. In years and locations with low to moderate weed pressure, such as in the 2014 study, differences among carrot varieties in weed competitiveness or tolerance were less apparent and therefore less relevant. Maximum carrot yield loss to weed competition among varieties was 28% in 2014. Yield loss in the presence of weeds was 15% or less with six of the nine carrot varieties. However, when weed pressure was intense in the 2015 study, both carrot plant density and carrot canopy development were inversely related to weed biomass. Carrot yield loss in the presence of weeds ranged from 38 to 87%. Despite correcting seeding populations for differences in germination among carrot varieties, carrot stand establishment varied greatly and would likely affect subsequent weed control measures such as timely cultivation or herbicide application. Future research efforts are warranted that consider carrot stand establishment factors and their relationship with integrated weed management programs.

Field experiments were conducted in 2008 and 2010 to determine crop tolerance and weed control efficacy of the POST herbicides bentazon, flumioxazin, and oxyfluorfen applied to direct-seeded dry bulb onions on organic soil. Postemergence application of oxyfluorfen at 0.071 kg ai ha−1 resulted in less than 20% onion injury when applied at the 2 and 4 onion leaf stages and provided good control of ladysthumb and common lambsquarters. Oxyfluorfen EC caused slightly higher visual injury than oxyfluorfen SC, but there was no difference in onion yield among the treatments. Application of flumioxazin at 0.036 of 0.072 kg ai ha−1 alone or in combination with pendimethalin ACS resulted in minimal onion injury and no yield reduction. Combining flumioxazin in a tank mix with pendimethalin EC, dimethenamid-P EC, or S-metolachlor EC resulted in significant onion injury and yield reduction. Flumioxazin plus S-metolachlor, dimethenamid-P, or pendimethalin improved ladysthumb control in one of two years. Bentazon applied at 0.56 kg ai ha−1 produced moderate onion injury and did not control yellow nutsedge adequately. Bentazon applied at 1.12 kg ai ha−1 provided good control of yellow nutsedge but caused serious onion injury and yield loss.

Two field experiments were conducted during 2018 at Paskeville and Arthurton, South Australia, to identify effective herbicide options for the control of thiocarbamate-resistant rigid ryegrass in wheat. Dose–response experiments confirmed resistance in both field populations (T1 and A18) of rigid ryegrass to triallate, prosulfocarb, trifluralin, and pyroxasulfone. T1 and A18 were 17.9- and 20-fold more resistant to triallate than susceptible SLR4. The level of resistance detected in T1 to prosulfocarb (5.9-fold) and pyroxasulfone (4-fold) was lower compared to A18, which displayed 12.1- and 7.8-fold resistance to both herbicides, respectively. Despite resistance, the mixture of two different preplant-incorporated (PPI) site-of-action herbicides improved rigid ryegrass control and wheat yield compared to a single PPI herbicide only. Prosulfocarb + triallate and prosulfocarb + S-metolachlor + triallate did not reduce rigid ryegrass seed set when compared to prosulfocarb applied alone at the higher rate (2,400 g ai ha–1). Pyroxasulfone + triallate PPI followed by glyphosate (1,880 g ai ha-1) as a weed seed set control treatment reduced rigid ryegrass seed production by 93% and 95% at both sites, respectively. These herbicides also significantly improved grain yield of wheat at Paskeville (22%) and Arthurton (38%) compared to the untreated.

Field experiments were established near Casselton and Fargo, ND, to evaluate the effect of aminopyralid soil residue on alfalfa, corn, soybean, and sunflower planted one or two growing seasons after treatment. At Fargo, ND, aminopyralid caused no injury or yield reduction to alfalfa, corn, and sunflower seeded 20 or 23 mo after treatment (MAT) in a silty clay soil. However, soybean yield was reduced when aminopyralid at 120 or 240 g ae ha−1 was fall- or spring-applied 20 or 23 mo before seeding. At Casselton, ND, aminopyralid injured alfalfa, soybean, and sunflower planted 8 and 11 MAT. Injury and yield reduction were less from treatments spring-applied than from those that were fall-applied. For example, aminopyralid at 120 g ha−1 applied in September caused 95, 94, and 100% injury to alfalfa, sunflower, and soybean, respectively, 8 MAT, whereas the same treatment applied in June caused 10, 8, and 44% injury 11 MAT. Aminopyralid at 120 g ha−1 continued to reduce soybean yield by an average of 45% at 20 MAT (fall-applied), but yield was similar to the control when aminopyralid was applied 23 mo before seeding (spring-applied). Warm soil with moderate moisture during the summer months appeared to be very important for degradation of aminopyralid. Corn was not affected by aminopyralid when seeded 8 or 11 MAT and appeared to be the best cropping option for land recently treated with aminopyralid. Aminopyralid applied at spot-treatment rates of 240 g ha−1 had long-term soil activity similar to picloram at 560 g ha−1.

Synthetic auxin herbicides are widely used because of their effective control of broadleaf weeds and safety in many turfgrass species. However, two synthetic auxin herbicides, triclopyr and aminocyclopyrachlor (AMCP; DPX-KJM44), are known to injure warm-season turfgrasses. Our objective was to quantify this injury through evaluations of turfgrass quality and turfgrass green cover in response to herbicide treatment. The results of this study indicate that relative to the labeled use rates of triclopyr (0.56 to 1.12 kg ae ha−1) and AMCP (0.053 kg ai ha−1), zoysiagrass is the only turfgrass tested with sufficient tolerance to the respective compounds for their use as weed-control agents. Bermudagrass and centipedegrass may be injured by triclopyr and AMCP at labeled rates, characterized by a reduction in turfgrass quality and green cover. St. Augustinegrass is not tolerant of either triclopyr or AMCP at labeled rates.

Studies were conducted under greenhouse conditions at Michigan State University and Texas Tech University to investigate the tolerance of Miscanthus × giganteus and Miscanthus sinensis to POST herbicides. Miscanthus sinensis and M. × giganteus were treated with 10 and 18 POST herbicide treatments, respectively. Plants were evaluated for injury as well as dry aboveground and belowground biomass production 28 days after treatment. Imazethapyr at 0.069 kg ai ha−1 caused 5% injury to M. sinensis, which was greater than the nontreated check. Imazethapyr, imazamox at 0.044 kg ai ha−1, and rimsulfuron at 0.017 kg ai ha−1 reduced aboveground biomass of M. sinensis compared with the nontreated check. Dicamba at 0.56 kg ai ha−1 and halosulfuron at 0.035 kg ai ha−1 resulted in M. sinensis aboveground biomass similar to the nontreated check. Injury exhibited by M. × giganteus was greater than the nontreated check with glyphosate at 0.84 kg ae ha−1 (54%), foramsulfuron at 0.037 kg ai ha−1 (32%), nicosulfuron at 0.035 kg ai ha−1 (28%), and imazamox at 0.044 kg ai ha−1 (10%). These treatments also yielded the lowest aboveground biomass values. The results of this study demonstrate that M. sinensis is more tolerant of the POST herbicides tested here than M.×x. giganteus. Several herbicide options may be available for weed management in M. sinensis and M. × giganteus stands following additional field trials to validate initial findings.

The development and spread of glyphosate-resistant (GR) horseweed has increased the use of dicamba as an alternative herbicide treatment. Research evaluated suspected glyphosate-resistant horseweed populations from DeKalb (GR-1) and Cherokee (GR-2) counties, Alabama, for response to glyphosate, dicamba, and glyphosate + dicamba. Populations used for resistance determination were tested at rosette and bolt growth stages. Glyphosate resistance evaluation treatments ranged from 0 to 36.0 kg ae ha−1. Data confirmed that GR-1 and GR-2 horseweed populations were 3.0 to 38 times more resistant to glyphosate than the susceptible population, according to population, data type, and growth stage at treatment. GR-1 and GR-2 populations were further evaluated for response to dicamba. Dicamba was applied at 0 to 1.12 kg ai ha−1, both with and without the addition of glyphosate at 1.12 kg ae ha−1. All populations had similar tolerance to dicamba, with the exception of GR-2 treated at the rosette growth stage, which had ~2-fold greater tolerance. When glyphosate was tank-mixed with dicamba, the response of GR populations was similar to that of dicamba alone. Therefore, any potential resistance-management benefit of tank-mixing dicamba with glyphosate may be negated when one is attempting to control GR horseweed. Conversely, adding glyphosate to dicamba drastically enhanced control of the susceptible population at both growth stages.

Soybean cultivars were grown in a hydroponic culture to evaluate tolerance to dimethenamid and metolachlor. Root length was reduced in 7 of the 32 cultivars with 50 ppb dimethenamid. ‘Asgrow 5403’ and ‘Bryan’ root lengths were reduced more than 20%. ‘Asgrow 6297’ was the only cultivar with greater than 12% root length reductions with 83 ppb metolachlor. The longest lateral root of all cultivars was shortened with dimethenamid. ‘NK-1460’ and Bryan expressed the most lateral root length reduction, at 73 and 62%, respectively. Asgrow 6297 was tolerant to dimethenamid, but lateral roots were reduced 16%. Metolachlor reduced the lateral root lengths of 12 cultivars, with Asgrow 5403, ‘Coker 6955,’ and Bryan reduced more than 20%. Bryan was the most sensitive cultivar to both herbicides, while ‘Brim’ was the most tolerant of cultivars evaluated.

A study was conducted to evaluate weed control with imazethapyr applied at different timings or a combination of timings with or without an early POST application of bensulfuron, bentazon plus aciflurofen, bispyribac, carfentrazone, halosulfuron, propanil plus molinate, or triclopyr in drill-seeded imidazolinone-resistant rice. Control of barnyardgrass, red rice, and alligatorweed was insufficient with imazethapyr at 87 g/ha PRE or at 53 g/ha late POST alone. Imazethapyr at 87 g/ha PRE followed by imazethapyr at 53 g/ha late POST controlled red rice 86% or greater but did not control hemp sesbania. The imazethapyr PRE followed by late POST combination was also weak on barnyardgrass and alligatorweed early in the season. The addition of an early POST application of the other herbicides to the imazethapyr combination improved overall weed control, especially hemp sesbania control. Among the early POST herbicides, bispyribac, carfentrazone, or propanil plus molinate were more effective in helping improve the overall weed control, resulting in better rice grain yield.

Laboratory experiments were conducted in 55-L aquaria to evaluate the efficacy of the aquatic herbicide endothall and the fungal pathogen Mycoleptodiscus terrestris (Gerd.) Ostazeski, applied alone and in combination against hydrilla. Treatments included 0.25, 0.50, and 1.25 mg ae/L endothall, 100, 200, and 400 colony-forming units (CFU)/ml M. terrestris, simultaneous integrated treatments of 0.25, 0.50, and 1.25 endothall + 100 or 200 CFU/ml M. terrestris, sequential integrated treatments of 100 and 200 CFU/ml M. terrestris + 0.25 and 0.50 mg ae/L endothall, and untreated controls. By 42 d after treatment (DAT), all treatments had significantly reduced shoot biomass levels of hydrilla compared with the untreated controls. Combining the two lowest herbicide rates with M. terrestris provided better hydrilla control than either treatment alone. Based on these results, an outdoor mesocosm study was conducted to evaluate the efficacy and selectivity of endothall and the pathogen applied alone and in combination against hydrilla, Illinois pondweed, American pondweed, and vallisneria. Treatments included 0.25 and 0.50 mg ae/L endothall, 100 and 200 CFU/ml M. terrestris, integrated treatments of 0.25 and 0.50 mg ae/L endothall + 100 and 200 CFU/ml M. terrestris, and untreated controls. Unlike the laboratory results, none of the treatments controlled hydrilla 100%. The combined treatments worked better than either treatment applied alone. By 42 DAT, all the combined treatments except 0.25 mg ae/L endothall + 100 CFU/ml M. terrestris had reduced above-ground hydrilla biomass by ≥ 90% compared with the untreated controls. All nontarget species sustained varying amounts of injury from endothall and M. terrestris applied alone or in combination.

Corn and soybean growers across Indiana were surveyed to assess their perceptions about the importance of preplant and POST weed control timing, focusing mainly on soybean production. Despite studies demonstrating the importance of planting into a clean field, almost a third of Indiana growers do not think it is important to plant into a weed-free seedbed and 74% do not use residual herbicides in glyphosate-resistant soybean production systems. Growers who farmed less than 200 ha were more likely to overestimate the ability of soybean to tolerate weed interference than growers who farmed more hectares. Growers who manage smaller farms were also more likely to use a one-pass weed control program than larger growers. This suggests that yield losses to weed interference may be greater for smaller farms than for larger farms. Weed size and density were the most common criteria used by growers to decide when to apply herbicides. This suggests that field scouting plays an important role in the decision-making process of growers. However, a substantial proportion of growers apply POST herbicides to large common lambsquarters and giant ragweed in an attempt to minimize the number of trips across the field for weed control. Delayed control of these species likely contributes to reduced crop yields, higher application rates, and to the survival of treated plants. Opportunities to improve control and increase yields through more optimal herbicide use appear possible for Indiana corn and soybean growers.

Herbicide-resistant weeds have impacted crop production throughout the United States, but the effect they have on extension programming has not been evaluated. In June 2007, 38 extension weed specialists throughout the United States, responded to a survey on herbicide-resistant (HR) weeds and the impact they are having on extension education programming. Survey results revealed that HR weeds have had a significant impact on extension programming particularly for agronomic crops. In the last 10 yr, agronomic weed specialists' extension programming was almost twice as likely to be impacted by the presence of HR weeds as compared to horticultural programming. In the next 5 yr, agronomic extension programming is twice as likely to be altered. Of 37 weed species reported, seven genera or species of weeds represented 80% of the major HR biotypes reported. These include Amaranthus species, horseweed, Setaria species, common lambsquarters, kochia, giant ragweed, and Lolium species. Five weed species (common ragweed, common lambsquarters, horseweed, kochia, and three foxtail species) exhibited weed by mode of action (MOA) interactions when evaluated as major or minor problems. Herbicide resistance problem severity differed for weed species, herbicide MOA, and crops. The results of this survey of university extension personnel confirm that HR weeds have impacted extension programming and will continue to impact programming in the future.

A host-specific fungus Colletotrichum truncatum strain 00-3B1 (Ct) was mixed with herbicides to improve the control of scentless chamomile, a noxious weed in western Canada. The compatibility of Ct conidia (spores) with herbicides was evaluated in vitro, and varying effects were observed with different products on spore germination. Clodinafop, glufosinate, MCPA, and 2,4-D ester were relatively benign and delayed the germination slightly, whereas dicamba, imazethapyr, metribuzin, and 2,4-D amine were noticeably more inhibitive. Bromoxynil, glyphosate, sethoxydim, and Merge® (spray adjuvant) were most inhibitive, showing >50% inhibition after 24 h. To determine potential synergy, Ct was applied at 7 × 106 spores/ml in tank mixtures with selected herbicides at 1× and 0.1× registered rates under greenhouse conditions. Combining Ct with MCPA, 2,4-D ester, clopyralid, or metribuzin at 1× rate resulted in synergistic or additive interaction on scentless chamomile, increasing weed control significantly when compared to Ct or herbicides applied alone. Similar applications of Ct with imazethapyr, 2,4-D amine, dicamba, or glyphosate were antagonistic. Treatments with Ct plus 1× metribuzin killed scentless chamomile completely, whereas neither Ct nor the herbicide alone caused plant death, suggesting the value of this tank mixture.