Thirty Suffolk cross wether sheep aged about 12 months and weighing 57 kg on average were used. They had been given previously a high energy diet to produce a fat body condition and average condition score of 3·7. Animals were allocated to an initial slaughter group (IS) or to four dietary treatment groups (UL, UH, CL and CH) based on sugar-beet feed (L) or white-fish meal (H) supplemented with chopped straw and pelleted, without (UL and UH) or with (CL and CH) the β-adrenergic agonist cimaterol, to provide an estimated 20 g (L) or 130 g (H) rumen undegradable protein per kg dry matter (DM). The sheep were offered 0·3 kg of the appropriate pelleted diet in addition to 200 to 400 g of chopped barley straw to provide proportionately 0·7 of the estimated metabolizable energy requirement for maintenance and, where appropriate, 2·5 mg cimaterol per day. Comparisons were made for the main effects of dietary protein intake and cimaterol and interactions between dietary protein and cimaterol.

The animals on dietary treatments were slaughtered after 49 days. The animals exhibited variable reductions in live weight and weights of carcass and non-carcass components in comparison with the IS group and in response to restricted dietary energy intake. The only effect due to the elevated dietary protein provision was a smaller loss in live weight compared with the low protein diet. In contrast, compared with responses recorded in its absence, treatment with cimaterol resulted in significantly greater values for weights of cold carcass, cross-sectional area o/m. longissimus dorsi and weights of DM, crude protein and ash in the carcass with significantly smaller weights for raw fleece. In comparison with the IS group, significantly smaller changes due to cimaterol were recorded for the weights of carcass crude protein and ash. Cimaterol treatment significantly increased the weight loss of fat in the carcass and in the perirenal and retroperitoneal, but not omental, depots as components of fleece-free non-carcass fat which was also significantly reduced in total. Interactions suggested that losses in carcass weight and DM in the presence of cimaterol were less and gains in carcass protein and ash greater, on the high than on the low protein diet. The results suggest that under conditions of energy undernutrition cimaterol may induce smaller losses or larger gains in carcass but not non-carcass, crude protein at the expense of fat depletion in carcass and non-carcass fat depots.

The effect of active immunization against somatostatin and β-agonist administration alone and in combination on growth, carcass composition and endocrine responses was investigated in young steers given a pelleted diet of dried grass and barley (0·7: 0·3) ad libitum for 16 weeks. Immunization alone increased growth rate by proportionately 0·10 but had no effect on plasma growth hormone (GH) or insulin-like growth factor-1 (IGF-1) concentrations (P > 0·10). Carcass protein content was unchanged but fat content tended to be increased. This was possibly associated with increased (P < 0·10) plasma insulin concentrations observed in these animals. Cimaterol administration significantly increased growth rate (P < 0·05) and carcass weight (P < 0·01). Carcass protein content was significantly (P < 0·01) increased and carcass fat was significantly (P < 0·001) reduced. Administration of cimaterol to immunized animals resulted in an enhancement of the repartitioning effect of the pagonist although growth rates were intermediate between the separate treatments. The mechanism by which this was achieved is unclear. Plasma IGF-1 concentrations tended (P < 0·10) to be lower in these animals compared with controls. Plasma insulin concentrations were also reduced (P < 0·05) in these animals. Both immunized animals and those given cimaterol alone showed an increased GH response to a single intravenous injection of growth hormone releasing factor (GRF) (P < 0·05). Animals given the combined treatment however showed a reduced response suggesting that the effects were not mediated through direct changes in GH secretion. The improved lean:fat ratio in the carcass was observed to be mediated through an enhancement of the lipolytic response to the /J-agonist with only small effects on protein accretion. It is suggested that immunoneutralization of somatostatin may increase the sensitivity of adipose tissue to lipolytic stimuli. This could occur through changes in the secretion of gastrointestinal or pancreatic hormones as a result of neutralization of the inhibitory influences of somatostatin. Further work is required to clarify these effects.

Forty-eight culled dairy cows were assigned to one of two treatment groups to investigate the effect of a β-adrenergic agonist in mature cattle. They were finished during a 63-day period on a concentrate diet, containing 0 or 4 mg/kg cimaterol. No food was given in the 20 h prior to slaughter.

Cimaterol had no effect on food intake, but significantly improved food conversion efficiency and increased live-weight gain, carcass weight and dressing proportion (P < 0·05). Cimaterol induced muscle hypertrophy as evidenced by an improved EUROP carcass classification and a higher longissimus thoracis (LT) cross-sectional area (P < 0·001). EUROP fat score and fat content in the carcass and the LT were lowered (P < 0·05). Cimaterol resulted in brighter meat (higher L-value: 37·0 v. 35·6; P<0·05) with a higher shear force value (64·0 v. 46·4 Newton; P<0·01) compared with control animals. It seems likely that mature cattle receiving cimaterol respond similarly to younger growing animals.

Twenty-one 6-month-old Israeli Holstein heifers were used to determine the effect of three 6-month feeding regimes on growth, blood concentration of insulin, prolactin and insulin-like growth factor-1 (IGF-1), attainment of puberty and milk production during first lactation. Feeding regimes were as follows. (1) Restricted: the heifers were given food during months 1, 2 and 4, 5 of an experimental period, to support live-weight (LW) gain of 0·35 and 0·50 kg/day, respectively and during months 3 and 6 the heifers were given food to support compensatory growth. (2) Control: the heifers were given food to support LW gain of 1·0 kg/day. (3) Control + C: the heifers were given the same as the control, with a daily supplement of 0·05 mg/kg LW cimaterol (C) for 4 months. The total LW gain of the restricted heifers during the 6 months of the trial was significantly lower than that of the control heifers. Cimaterol improved growth rate only during the first 2 months of its application and its withdrawal was associated with severe LW gain retardation. The feeding regime employed in the restricted treatment was associated with a significant reduction in serum concentrations of insulin, prolactin and IGF-1 during the first restricted phase, followed by an elevation in the first compensatory phase. During the second restriction-compensation cycle, only the serum concentration of prolactin was significantly reduced. Cimaterol addition was also associated with a reduced blood concentration of the hormones. The animals in the restricted, control and control + C groups attained puberty at LW of 249·2, 277·6 and 304·9 kg (P <0·05), respectively. No effect of the treatments on milk yield was observed. The effect of the feeding regimes on skeletal growth and on metubolizable energy efficiency for growth is discussed.

The action of intravenous infusion of the βagonist cimaterol(2.5 mg/d) on whole-body N retention and protein synthesis in peripheral tissues was examined in growing sheep. Wool growth was determined from skin patch clippings and adjusted to total fibre production. Protein synthesis was measured, using sequential large dose injections of [ l-I3C]valine, leucine and phenylalanine and then [ring-d,]-phenylalanine, on biopsy samples from skin and m. longissirnus dovsi taken before β-agonist administration, at day 3 and day 15 of cimaterol infusion, and 15 d after withdrawal of the drug. Cimaterol increased total N retention by 1.9–2.3 g N/d (P < 0.01) over three successive 5 d periods. In contrast, wool growth was significantly reduced by 0.7 g N/d (P < 0.001) and the proportion of total N retained in wool declined from 0.71 to 0.25 (P < 0.01). The reduction in woo1 growth was accompanied by a decrease in protein fractional synthesis rate (FSR) in skin (11.6 v. 6.3%/d, P < 0.01). Muscle protein FSR, on the other hand, was markedly stimulated during cimaterol infusion (1.45 v. 3.01 %/d, P < 0.001) as was RNA concentration (P < 0.001), RNA:protein (P < 0.001) and protein:DNA (P < 0.05). The estimated increase in total protein synthesis in muscle (+ 24 to 30 g/d) due to cimaterol administration was counterbalanced by reductions for skin (-25 to 27 g/d); this may account for the lack of changes in whole-body protein synthesis following β-agonist administration reported in other studies. Although N retention rapidly returned to control values following withdrawal of the drug, both wool growth and skin protein synthesis remained depressed, while muscle protein FSR declined, but not to pre-treatment values. These results suggest a persistent action of cimaterol, but whether this is a function of residue concentrations or long-term metabolic responses is not known

The effect of fish-meal supplementation or cimaterol administration on the composition, cellularity and metabolism of subcutaneous and perirenal adipose tissue was examined in young Friesian steers given grass silage. Animals (approx. 117 kg live weight) received either silage alone (group C; no. = 6) or supplemented with fish meal (150 g/kg silage dry matter; group FM; no. = 6) or cimaterol (0·06 mg/kg body weight per day) administered via osmotic minipumps (group CIM; no. = 6) for a period of 6 weeks. Samples of perirenal and subcutaneous adipose tissue obtained at slaughter were used for determination of tissue composition and in vitro rates of lipogenesis, lipolysis and substrate oxidation. FM animals showed significantly greater live-weight gains (0·61 kg/day) compared with C (0·21 kg/day, P < 0·001) while CIM animals had significantly lower rates of gain (0·04 kg/day, P < 0·05). This was considered to be related to increased heat loss from the β-agonist-treated animals as a result of very cold weather encountered during the experimental period. CIM administration reduced adipocyte size suggesting an increased number of cells per g tissue. Both lipolytic and lipogenic rates were reduced by cimaterol and there was some evidence that the response to insulin was diminished, at least with respect to substrate oxidation. FM increased lipogenesis from acetate and acetate oxidation rates in subcutaneous adipose tissue with similar trends in perirenal tissue. The results indicate that both fish-meal supplementation and cimaterol administration caused substantial changes in adipose tissue metabolism as determined using in vitro procedures, even though the magnitude and direction of the changes were not in strict accord with estimates of net fat accretion from related in vivo studies.

An experiment was conducted to evaluate the effect of dietary cimaterol, a β-adrenergic agonist, on growth, carcass characteristics and pelt quality of kit mink. The 40 standard dark and 40 sapphire mink kits were assigned to a control or to one of three treatment groups (five males and five females of each colour phase per group). Treatment consisted of the inclusion of 0·5, 2 and 5 mg cimaterol per kg (dry) of a standard ranch mink diet (control). All animals were weighed at the initiation of the experiment (24 July) and at 28-day intervals until 16 October. At slaughter (12 December) body weights were recorded and pelts measured for length and evaluated for fur colour and quality by experienced fur graders. Samples of fur from similar sites on each animal were removed for measurement of guard hair and underfur diameter. Frozen carcasses were analysed for protein and fat content. Mink final weight decreased with increasing concentrations of dietary cimaterol (P < 0·05). Pelt length, which was found to be highly correlated with body size, was also reduced by cimaterol treatment. Feeding 2 and 5 mg cimaterol per kg diet to male kits, and 5 mg/kg to female kits caused a significant reduction in the proportion of carcass fat and increased the proportion of carcass protein (P < 0·05). Fur fibre diameter for both guard hair and underfur was not significantly affected by dietary cimaterol. There was also no significant effect of cimaterol treatment on fur quality. Cimaterol treatment did not result in diversion of nutrients from body proteins to the synthesis of fur and pelt proteins. Results of this research indicate that oral administration of cimaterol to mink will be of little practical value in fur production.

Twenty-four Finn ♂ × Dorset Down ♀ wether lambs about 10 weeks and weighing on average 18 kg were allocated to treatment groups as follows: untreated controls (U); 15 mg oestradiol-17β (OE) implanted subcutaneously (O); cimaterol (2 mg/kg dry matter (DM)) in the diet (C); and 15 mg OE implant + cimaterol (2 mg/kg DM in the diet) (OC). The lambs were offered a diet, restricted to 38 g/kg live weight (LW), and which contained an estimated 12·5 MJ metabolizable energy and 137 g crude protein per kg DM for the experimental period of 62 or 63 days. Comparisons were made for the main effects of OE and cimaterol and also interactions between OE and cimaterol. Treatment with OE, on average, resulted in greater LW gain, DM intake, empty body weight (EBW), body length and chest depth, weight of shoulder and loin joints and carcass crude protein, but had no effect on fat deposition in carcass or depots. As a proportion of EBW, the weights of the pituitary gland and accessory vesicular glands were increased, as was teat length in lambs treated with OE. Cimaterol treatment, on average, increased LW gain, EBW, killing-out ratio, carcass weight and its weight of crude protein and, unlike OE, decreased the weight of the pituitary gland as a proportion of EBW and reduced the weight and proportion of fat in the carcass and perirenal and retroperitoneal depots. Increases in the cross-sectional area of m. longissimus dorsi and in the weight of selected major and minor commercial joints were also obtained in cimaterol-treated lambs. The presence of significant interactions indicated that there was incomplete or no additivity between the effects of cimaterol and OE for LW gain, cross-sectional area of m. longissimus dorsi and weights of carcass and carcass crude protein, omental fat and of certain of the dissected joints. These results suggest either that the mechanisms of action of oestradiol-17β and cimaterol are not independent or that they are affected by similar metabolic or physiological processes which limit the ability of castrated male sheep to exhibit a positive response.

The effects of sex (wethers v. ewes), diet (chopped lucerne hay v. lucerne-barley pellets) and cimaterol on energy utilization by Suffolk cross lambs were determined by comparative slaughter. Quadratic regression of energy retention (RE) on metabolizable energy intake (MEI) enabled estimation of maintenance energy requirements (Em), efficiencies of gain (ktotal) and maximum rates of gain (REMAX). Regressions using RE in fat and protein v. MEI yielded analogous parameters for fat and protein deposition (Em fat, kfat, REMAX fat and Emprotcin, kprolein, REMAXprotcin respectively). Em was lower in wethers than ewes (455 v. 510 kJ/kg M0·75 per day respectively), but was unaffected by diet or cimaterol. Sex and cimaterol did not affect ktotai. which was higher in lambs given pellets compared with lambs given hay (0·417 v. 0·224 respectively). Similarly, REMAX was higher in lambs given pellets than in lambs given hay (326 v. 114 kJ/kg 0·75 per day respectively). None of the groups differed significantly in the parameters of fat deposition, which averaged 480 kJ/kg 0·75 per day for Em fal, 0·224 for ktat, and 250 kJ/kg M0·75 per day for REMAX, fat- Em.protein was lower in wethers than in ewes (466 v. 569 kJ/kg 0·075 per day, respectively), and was further reduced by cimaterol (418 and 507 kJ/kg 0·75 per day for wethers and ewes respectively). Estimates of kprotcin were higher in wethers than in ewes (0·091 v. 0·064 respectively), and were increased by cimaterol (0·115 and 0·089 for wethers and ewes respectively). Similarly REMAX protein was higher in wethers than in ewes (47 v. 37 kJ/kg 0·75 per day respectively), and was increased by cimaterol (58 and 48 kJ/kg 0·75 per day for wethers and ewes respectively). The repartitioning action of cimaterol was additive with effects of diet, intake and sex.