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Effect of fish-meal supplementation and β-agonist administration on adipose tissue metabolism in steers given silage

Published online by Cambridge University Press:  25 May 2016

J. M. Dawson ,
C. P. Essex ,
A. Walsh ,
D. E. Beever ,
M. Gill  and
P. J. Buttery
J. M. Dawson
Affiliation:
Department of Applied Biochemistry and Food Science, University of Nottingham, Faculty of Agricultural and Food Sciences, Sutton Bonington Campus, Loughborough LE12 5RD
C. P. Essex
Affiliation:
Department of Applied Biochemistry and Food Science, University of Nottingham, Faculty of Agricultural and Food Sciences, Sutton Bonington Campus, Loughborough LE12 5RD
A. Walsh
Affiliation:
Institute for Grassland and Environmental Research, Hurley, Maidenhead SL6 5LR
D. E. Beever
Affiliation:
Institute for Grassland and Environmental Research, Hurley, Maidenhead SL6 5LR
M. Gill
Affiliation:
Institute for Grassland and Environmental Research, Hurley, Maidenhead SL6 5LR
P. J. Buttery
Affiliation:
Department of Applied Biochemistry and Food Science, University of Nottingham, Faculty of Agricultural and Food Sciences, Sutton Bonington Campus, Loughborough LE12 5RD
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Abstract

The effect of fish-meal supplementation or cimaterol administration on the composition, cellularity and metabolism of subcutaneous and perirenal adipose tissue was examined in young Friesian steers given grass silage. Animals (approx. 117 kg live weight) received either silage alone (group C; no. = 6) or supplemented with fish meal (150 g/kg silage dry matter; group FM; no. = 6) or cimaterol (0·06 mg/kg body weight per day) administered via osmotic minipumps (group CIM; no. = 6) for a period of 6 weeks. Samples of perirenal and subcutaneous adipose tissue obtained at slaughter were used for determination of tissue composition and in vitro rates of lipogenesis, lipolysis and substrate oxidation. FM animals showed significantly greater live-weight gains (0·61 kg/day) compared with C (0·21 kg/day, P < 0·001) while CIM animals had significantly lower rates of gain (0·04 kg/day, P < 0·05). This was considered to be related to increased heat loss from the β-agonist-treated animals as a result of very cold weather encountered during the experimental period. CIM administration reduced adipocyte size suggesting an increased number of cells per g tissue. Both lipolytic and lipogenic rates were reduced by cimaterol and there was some evidence that the response to insulin was diminished, at least with respect to substrate oxidation. FM increased lipogenesis from acetate and acetate oxidation rates in subcutaneous adipose tissue with similar trends in perirenal tissue. The results indicate that both fish-meal supplementation and cimaterol administration caused substantial changes in adipose tissue metabolism as determined using in vitro procedures, even though the magnitude and direction of the changes were not in strict accord with estimates of net fat accretion from related in vivo studies.

Keywords

cattlecimaterolfish meallipogenesislipolysis
Type
Research Article
Information
Animal Production , Volume 57 , Issue 3 , December 1993 , pp. 397 - 406
Copyright
Copyright © British Society of Animal Science 1993

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References

Beermann, D. H., Butler, W. R., Hogue, D. E., Fishell, V. K., Dalrymple, R. H., Ricks, C. A. and Scanes, C. G. 1987. Cimaterol-induced muscle hypertrophy and altered endocrine status in lambs, journal of Animal Science 65: 1514–1524.Google Scholar
Beever, D. E., Gill, M., Dawson, J. M. and Buttery, P. J. 1990. The effect of fishmeal on the digestion of grass silage by growing cattle. British journal of Nutrition 63: 489–502.Google Scholar
Brooks, B. J., Arch, J. R. S. and Newsholme, E. A. 1982. Effects of some hormones on the rate of the triacylglycerol/fatty acid substrate cycle in adipocytes and epididymal fat pads. FEES Letters 146: 327–330.CrossRefGoogle ScholarPubMed
Buttery, P. J., Dawson, J. M. and Harper, J. M. M. 1990. Exploiting the physiology of growth. Proceedings of the New Zealand Society of Animal Production 50: 59–72.Google Scholar
Dawson, J. M., Buttery, P. J., Gill, M. and Beever, D. E. 1989. Manipulation of lean deposition in forage-fed cattle. Asian-Australasian Journal of Animal Science 2: 243–245.Google Scholar
Dawson, J. M., Buttery, P. J., Lammiman, M. J., Soar, J. B., Essex, C. P., Gill, M. and Beever, D. E. 1991. Nutritional and endocrinological manipulation of lean deposition in forage-fed steers. British Journal of Nutrition 66: 171–185.Google Scholar
Dole, V. P. and Meinertz, H. 1960. Microdetermination of long chain fatty acids in plasma and tissues. journal of Biological Chemistry 235: 2595–2599.CrossRefGoogle ScholarPubMed
Eadera, J., Dalrymple, R. H., Delay, R. L., Ricks, C. A. and Romsos, D. R. 1987. Cimaterol, a novel β-agonist, selectively stimulates white adipose tissue lipolysis and skeletal muscle lipoprotein lipase activity in rats. Federation Proceedings 46: 1020 (abstr.).Google Scholar
Eisemann, J. H., Huntington, G. B. and Ferrell, C. L. 1988. Effects of dietary clenbuterol on metabolism of the hindquarters in steers. journal of Animal Science 66: 342–353.Google Scholar
Etherton, T. D., Thompson, E. H. and Allen, C. E. 1977. Improved techniques for studies of adipocyte cellularity and metabolism. journal of Lipid Research 18: 552–557.Google Scholar
Fain, J. N. and Garcia-Sainz, J. A. 1983. Adrenergic regulation of adipocyte metabolism. journal of Lipid Research 24: 945–966.Google Scholar
Fennessy, P. F., McEwan, J. C., Lord, E. A., Greer, G. J., Bain, W. E., Johnstone, P. D., Knowler, M. A., Dalrymple, R. H. and Ingle, D. L. 1990. Effect of cimaterol implants on lamb growth and carcass traits. New Zealand Journal of Agricultural Research 33: 413–427.Google Scholar
GENSTAT 5 Committee. 1987. GENSTAT 5 Reference Manual. Clarendon Press, Oxford.Google Scholar
Gill, M., Beever, D. E., Buttery, P. J., England, P., Gibb, M. J. and Baker, R. D. 1987. The effect of oestradiol-17β implantation on the response in voluntary intake, liveweight gain and body composition, to fishmeal supplementation of silage offered to growing calves. journal of Agricultural Science, Cambridge 108: 9–16.CrossRefGoogle Scholar
Hausdorff, W. P., Caron, M. G. and Lefkowitz, R. J. 1990. Turning off the signal: desensitization of β-adrenergic receptor function. FASEB Journal 4: 2881–2889.Google Scholar
Hood, R. L., Thompson, E. H. and Allen, C. E. 1972. The role of acetate, propionate and glucose as substrates for lipogenesis in bovine tissues. International Journal of Biochemistry 3: 598–606.CrossRefGoogle Scholar
Hu, C. Y., Suryawan, A., Forsberg, N. E., Dalrymple, R. H. and Ricks, C. A. 1988. Effect of cimaterol on sheep adipose tissue lipid metabolism. journal of Animal Science 66: 1393–1400.Google Scholar
Kim, Y. S., Lee, Y. B., Garrett, W. N. and Dalrymple, R. H. 1989. Effects of cimaterol on nitrogen retention and energy utilisation in lambs. journal of Animal Science 67: 674–681.CrossRefGoogle ScholarPubMed
Kirsch, D. M., Baumgarten, M., Deufel, T., Rinninger, F., Kemmler, W. and Haring, H. U. 1983. Catecholamineinduced insulin resistance of glucose transport in isolated rat adipocytes. Biochemical Journal 216: 737–745.CrossRefGoogle ScholarPubMed
Liu, C. Y., Boyer, J. L. and Mills, S. E. 1989. Acute effects of beta-adrenergic agonists on porcine adipocyte metabolism in vitro. journal of Animal Science 67: 2930–2936.CrossRefGoogle ScholarPubMed
Merkel, R. A., Dickerson, P. S., Johnson, S. E., Burkett, R. L., Burnett, R. J., Schroeder, A. L., Bergen, W. G. and Anderson, D. B. 1987. The effect of ractopamine on lipid metabolism in pigs. Federation Proceedings 46: 1177 (abstr.).Google Scholar
Mersmann, H. J. 1987. Acute metabolic effects of adrenergic agonists in swine. American Journal of Physiology 252: E85–E95.Google ScholarPubMed
Mersmann, H. J. 1992. Regulation of porcine adipose tissue lipolytic activity by adrenergic agonists and antagonists. Comparative Biochemistry and Physiology 102C: 407–411.Google Scholar
Miller, M. F., Garcia, D. K., Coleman, M. E., Ekeren, P. A., Lunt, D. K., Wagner, K. A., Procknor, M., Welsh, T. H. and Smith, S. B. 1988. Adipose tissue, longissimus muscle and anterior pituitary growth and function in clenbuterol-fed heifers. journal of Animal Science 66: 12–20.Google Scholar
Mills, S. E. and Orcutt, A. L. 1989. Clenbuterol-induced desensitization in murine adipocytes: relationship to in vivo effectiveness. Domestic Animal Endocrinology 6: 51–58.CrossRefGoogle ScholarPubMed
Mills, S. E. and Liu, C. Y. 1990. Sensitivity of Iipolysis and lipogenesis to dibutyryl-cAMP and β-adrenergic agonists in swine adipocytes in vitro. journal of Animal Science 68: 1017–1023.Google Scholar
Moloney, A., Allen, P., Joseph, R. and Tarrant, V. 1991. Influence of beta-adrenergic agonists and similar compounds on growth. In Advances in meat research, vol. 7 (ed. Pearson, A. M. and Dutson, T. R.), pp. 455–513. Elsevier Science, Essex.Google Scholar
Moser, R. L., Dalrymple, R. H., Cornelius, S. G., Pettigrew, J. E. and Allen, C. E. 1986. Effect of cimaterol (CL263,780) as a repartitioning agent in the diet for finishing pigs. journal of Animal Science 62: 21–26.CrossRefGoogle Scholar
O'Connor, R. M., Butler, W. R., Finnerty, K. D., Hogue, D. E. and Beermann, D. H. 1991. Acute and chronic hormone and metabolite changes in lambs fed the betaagonist, cimaterol. Domestic Animal Endocrinology 8: 537–548.Google Scholar
Pell, J. M., Elcock, C., Harding, R. L., Morrell, D. J., Simmonds, A. D. and Wallis, M. 1990. Growth, body composition, hormonal and metabolic status in lambs treated long-term with growth hormone. British Journal of Nutrition 63: 431–445.CrossRefGoogle ScholarPubMed
Peterla, T. A., Ricks, C. A. and Scanes, C. G. 1987. Comparison of β-agonist stimulation of in vitro Iipolysis in rat and sheep. Federation Proceedings 46: 1021 (abstr.).Google Scholar
Reeds, P. J. and Mersmann, H. J. 1991. Protein and energy requirements of animals treated with β-adrenergic agonists: a discussion. journal of Animal Science 69:1532–1550.Google Scholar
Sanderson, R. 1992. The effects of supplements of fishmeal and specific amino acids on the growth and efficiency of young steers given grass silage. PhD thesis, University of Reading.Google Scholar
Taylor, A. W., Garrod, J., McNulty, M. E. and Secord, D. C. 1973. Regenerating epididymal fat pad size and number after exercise training and three different feeding patterns. Growth 37: 345–354.Google Scholar
Thornton, R. F., Tume, R. K., Payne, G., Larsen, T. W., Johnson, G. W. and Hohenhaus, M. A. 1985. The influence of the β2-adrenergic agonist, clenbuterol on lipid metabolism and carcass composition of sheep. Proceedings of the New Zealand Society for Animal Production 45: 97–101.Google Scholar
Truscott, T. G., Wood, J. D. and Macfie, H. J. H. 1983. Fat deposition in Hereford and Friesian steers. 1. Body composition and partitioning of fat between depots. journal of Agricultural Science, Cambridge 100: 257–270.Google Scholar
Varley, H. 1967. Practical clinical biochemistry. 4th ed. Wiley.Google Scholar
Vernon, R. G. 1975. Effect of dietary safflower oil upon lipogenesis in neonatal lamb. Lipids 10: 284–289.CrossRefGoogle ScholarPubMed
Vernon, R. G. 1980. Lipid metabolism in the adipose tissue of ruminant animals. Progress in Lipid Research 19: 23–106.Google Scholar
Ward, J. R., Henricks, D. M., Jenkins, T. C. and Bridges, W. C. 1992. Serum hormone and metabolite concentrations in fasted young bulls and steers. Domestic Animal Endocrinology 9: 97–103.Google Scholar
Walsh, A., Gill, M. and Beever, D. E. 1990. The effect of fish meal supplementation on in-vitro subcutaneous adipose tissue metabolism in young cattle fed silage. Animal Production 50: 584 (abstr.).Google Scholar