Research was conducted from 2011 to 2015 to determine the effect of herbicide strategy on efficacy and evolution of herbicide resistance in weeds in a continuous glyphosate- and dicamba-resistant (GDr) soybean system. The nine herbicide strategies included sequential applications of glyphosate only, glyphosate plus dicamba with or without acetochlor, PRE application of residual herbicides with POST glyphosate or non-glyphosate herbicides, and their biennial rotation with one another. Giant foxtail and horseweed were the least problematic during all growing seasons. An increase in horseweed was observed by the end of the experiment especially in the plots where POST glyphosate was not used with PRE application of residual herbicides. Giant ragweed evolved resistance to glyphosate over a 4-yr period of selection with strategies that predominantly included PRE and POST glyphosate. Herbicide use strategies that included glyphosate-only and PRE application of residual herbicides fb POST glyphosate annually or in a biennial rotation were ineffective in controlling giant ragweed and glyphosate-resistant (GR) common waterhemp. Over the years, application of PRE herbicide mixtures before POST glyphosate application improved weed control and soybean yields compared with the glyphosate-only strategy. During all growing seasons, the greatest yield and reduction in total weed density before harvest was provided by herbicide use strategies that included glyphosate plus dicamba annually or in a biennial rotation regardless of the inclusion of acetochlor POST. Dicamba proved to be a valuable addition to improve the control of GR weeds. GDr soybean will provide growers with a new option for managing resistant weeds, but it needs to be used with caution, as multiple resistance in weeds, including waterhemp and giant ragweed, is already widespread.

Glyphosate-resistant (GR) common waterhemp (CW) is a localized weed in Ontario and one of the most problematic weeds in the US Corn Belt. First confirmed in Ontario in 2014, GR CW has now been confirmed in forty fields in three counties in Ontario as of 2015. Historically, the primary POST herbicides used for the control of CW in soybean were glyphosate, acifluorfen and fomesafen, but resistance to all three has been confirmed in many US states. Research was conducted in 2015 and 2016 to determine the control of GR CW with some of the new herbicide-resistant soybean technologies including glufosinate (LibertyLink), 2,4-D and glyphosate (Enlist), and isoxaflutole, mesotrione, and glufosinate (HPPD-resistant). Glyphosate-resistant CW was controlled (≥90%) all season with a two-pass weed control system across all herbicide-resistant soybean technologies evaluated. The two-pass weed control system in this research is defined as a PRE herbicide followed by a POST herbicide. At 12 WAA, the two-pass programs in LibertyLink, Enlist, and HPPD-resistant systems controlled GR CW up to 98, 98, and 92%, respectively, and reduced GR CW densities to 0 to 2% of the weedy control at 4 WAA. The two-pass programs provided greater GR CW control than PRE or POST herbicides alone. This study found that the use of two-pass weed control programs in glufosinate-resistant, glyphosate DMA/2,4-D choline-resistant and HPPD-resistant soybean can provide excellent control of GR CW, and can be valuable tools to reduce the selection intensity for herbicide-resistant weeds. Through the rotational use of different technologies, growers may be able to better manage their weed populations in reducing the risk of resistance when compared to the use of one herbicide repeatedly.

A field study was conducted for the 2014 and 2015 growing season in Arkansas, Indiana, Illinois, Missouri, Ohio, and Tennessee to determine the effect of cereal rye and either oats, radish, or annual ryegrass on the control of Amaranthus spp. when integrated with comprehensive herbicide programs in glyphosate-resistant and glufosinate-resistant soybean. Amaranthus species included redroot pigweed, waterhemp, and Palmer amaranth. The two herbicide programs included were: a PRE residual herbicide followed by POST application of foliar and residual herbicide (PRE/POST); or PRE residual herbicide followed by POST application of foliar and residual herbicide, followed by another POST application of residual herbicide (PRE/POST/POST). Control was not affected by type of soybean resistance trait. At the end of the season, herbicides controlled 100 and 96% of the redroot pigweed and Palmer amaranth, respectively, versus 49 and 29% in the absence of herbicides, averaged over sites and other factors. The PRE/POST and PRE/POST/POST herbicide treatments controlled 83 and 90% of waterhemp at the end of the season, respectively, versus 14% without herbicide. Cover crop treatments affected control of waterhemp and Palmer amaranth and soybean yield, only in the absence of herbicides. The rye cover crop consistently reduced Amaranthus spp. density in the absence of herbicides compared to no cover treatment.

Glyphosate-resistant (GR) common waterhemp has become a significant problem weed in Nebraska and several Midwestern states. Several populations of GR common waterhemp are also resistant to acetolactate synthase (ALS)-inhibiting herbicides, making them difficult to control with POST herbicides in GR soybean. Glufosinate-resistant (GFR) soybean is an alternate system for controlling GR common waterhemp, justifying the need for evaluating glufosinate-based herbicide programs. The objectives of this study were to compare POST-only herbicide programs (including one-pass and two-pass POST programs) with PRE followed by (fb) POST herbicide programs for control of GR common waterhemp in GFR soybean and their effect on common waterhemp density, biomass, and soybean yield. Field experiments were conducted in 2013 and 2014 near Fremont, NE in a grower’s field infested with GR common waterhemp. Glufosinate applied early- and late-POST provided 76% control of GR common waterhemp at 14 d after late-POST (DALPOST) compared with 93% control with a PRE fb POST program when averaged across treatments. The PRE application of chlorimuron plus thifensulfuron plus flumioxazin, S-metolachlor plus fomesafen or metribuzin, saflufenacil plus dimethenamid-P fb glufosinate provided ≥95% control of common waterhemp throughout the growing season, reduced common waterhemp density to ≤2.0 plants m─2, caused ≥94% biomass reduction, and led to 1,984 to 2,210 kg ha─1 soybean yield. Averaged across treatments, the PRE fb POST program provided 82% common waterhemp control at soybean harvest, reduced density to 23 plants m─2 at 14 DALPOST, and caused 86% biomass reduction and 1,803 kg ha─1 soybean yield compared with 77% control, 99 plants m─2, 53% biomass reduction, and 1,190 kg ha─1 yield with POST-only program. It is concluded that PRE fb POST programs with multiple effective modes of action are available for control of GR common waterhemp in GFR soybean.

Field experiments were conducted in Platte County, Missouri, during 2006 and 2007 to evaluate PRE, POST, and PRE followed by (fb) POST herbicide programs for the control of glyphosate-resistant waterhemp in soybean. All PRE fb POST treatments resulted in at least 66 and 70% control of glyphosate-resistant waterhemp in 2006 and 2007, respectively. Control of glyphosate-resistant waterhemp was less than 23% with lactofen and acifluorfen in 2006, but at least 64% in 2007. Variability in control likely resulted from differences in trial locations and a population of protoporphyrinogen oxidase (PPO)–resistant waterhemp at the Platte County site in 2006 compared with 2007. In both years, glyphosate resulted in less than 23% control of glyphosate-resistant waterhemp and provided the least control of all herbicide programs. Programs containing PRE herbicides resulted in waterhemp densities of less than 5 plants/m2, whereas the POST glyphosate treatment resulted in 38 to 70 plants/m2. Waterhemp seed production was reduced at least 78% in all PRE fb POST programs, from 55 to 71% in POST programs containing lactofen and acifluorfen and by only 21% in the POST glyphosate treatment. Soybean yields corresponded to the level of waterhemp control achieved in both years, with the lowest yields resulting from programs that provided poorest waterhemp control. PRE applications of S-metolachlor plus metribuzin provided one of the highest net incomes in both years and resulted in $271 to $340/ha greater net income than the glyphosate-only treatment. Collectively, the results from these experiments illustrate the effectiveness of PRE herbicides for the control of glyphosate-resistant waterhemp in glyphosate-resistant soybean and the inconsistency of PPO-inhibiting herbicides or PPO-inhibiting herbicide combinations for the control of waterhemp populations with multiple resistance to glyphosate and PPO-inhibiting herbicides.

Research was conducted to determine the competitiveness and fitness of a protoporphyrinogen oxidase (protox)-resistant common waterhemp biotype. Protox-resistant and protox-susceptible biotypes were grown under noncompetitive and competitive arrangements in the greenhouse. In the noncompetitive study, a single plant of each biotype was planted separately in 15-cm-diam pots. Photosynthesis, leaf area, and plant biomass were measured 10, 20, 30, and 40 d after transplanting (DATP). In general, photosynthesis rate and plant biomass were similar between biotypes. However, the protox-resistant biotype had higher leaf area than the susceptible biotype at 20, 30, and 40 DATP. A replacement series study was conducted in the greenhouse to evaluate the relative competitiveness of protox-resistant and protox-susceptible common waterhemp. Photosynthesis, leaf area, plant height, and plant biomass were measured 7, 14, 21, and 28 DATP. Protox-resistant and -susceptible common waterhemp were equally competitive 28 DATP. Relative crowding coefficient values 28 DATP were 0.86, 0.89, 1.09, and 1.13 for photosynthesis, leaf area, plant height, and plant biomass, respectively. This suggests protox-resistant and -susceptible common waterhemp were equally competitive and the frequency of protox-resistant biotype is unlikely to decrease in the absence of protox–herbicide selection pressure.

Because of the increasing number of glyphosate-resistant weeds, alternate herbicide-resistant crops and herbicides with different modes of action are required to protect crop yield. Glufosinate is a broad-spectrum POST herbicide for weed control in glufosinate-resistant crops, including soybean. The objective of this study was to compare herbicide programs with glufosinate applied singly at late-POST (LPOST) or sequentially at early POST (EPOST) followed by (fb) LPOST applications and PRE herbicides fb EPOST/LPOST glufosinate alone or tank-mixed with acetochlor, pyroxasulfone, or S-metolachlor in glufosinate-resistant soybean. A field experiment was conducted at the South Central Agriculture Laboratory in Clay Center, NE, in 2012 and 2013. Glufosinate applied in a single LPOST or sequential EPOST fb LPOST application controlled common lambsquarters, common waterhemp, eastern black nightshade, green foxtail, large crabgrass, and velvetleaf ≤ 82% and resulted in a weed density of 6 to 10 plants m−2 by the end of the season. Flumioxazin-, saflufenacil-, or sulfentrazone-based premixes provided 84 to 99% control of broadleaf and grass weeds tested in this study at 15 d after PRE application and a subsequent LPOST application of glufosinate alone controlled broadleaf and grass weeds 69 to 93% at harvest, depending on the herbicide program and weed species being investigated. The PRE application of sulfentrazone plus metribuzin fb EPOST glufosinate tank-mixed with acetochlor, pyroxasulfone, or S-metolachlor controlled the tested broadleaf and grass weeds ≥ 90%, reduced density to ≤ 2 plants m−2, and reduced weed biomass to ≤ 10 g m−2 and produced soybean yields of ≥ 4,450 and 3,040 kg ha−1 in 2012 and 2013, respectively. Soybean injury was 0 to 20% from PRE or POST herbicides, or both and was inconsistent, but transient, during the 2-yr study, and it did not affect soybean yield. Sulfentrazone plus metribuzin applied PRE fb glufosinate EPOST tank-mixed with acetochlor, pyroxasulfone, or S-metolachlor provided the highest level of weed control throughout the growing season and increased soybean yield compared with a single LPOST or a sequential EPOST fb LPOST glufosinate application. Additionally, these herbicide programs provide four distinct mechanisms of action that constitute an effective weed-resistance management strategy in glufosinate-resistant soybean.

Propane flaming could be an effective alternative tool for weed control in organic cropping systems. However, response of major weeds to broadcast flaming must be determined to optimize its proper use. Therefore, field experiments were conducted at the Haskell Agricultural Laboratory, Concord, NE in 2007 and 2008 using six propane doses and four weed species, including green foxtail, yellow foxtail, redroot pigweed, and common waterhemp. Our objective was to describe dose–response curves for weed control with propane. Propane flaming response was evaluated at three different growth stages for each weed species. The propane doses were 0, 12, 31, 50, 68, and 87 kg ha−1. Flaming treatments were applied utilizing a custom-built flamer mounted on a four-wheeler (all-terrain vehicle) moving at a constant speed of 6.4 km h−1. The response of the weed species to propane flaming was evaluated in terms of visual ratings of weed control and dry matter recorded at 14 d after treatment. Weed species response to propane doses were described by log-logistic models relating propane dose to visual ratings or plant dry matter. Overall, response of the weed species to propane flaming varied among species, growth stages, and propane dose. In general, foxtail species were more tolerant than pigweed species. For example, about 85 and 86 kg ha−1 were the calculated doses needed for 90% dry matter reduction in five-leaf green foxtail and four-leaf yellow foxtail compared with significantly lower doses of 68 and 46 kg ha−1 of propane for five-leaf redroot pigweed and common waterhemp, respectively. About 90% dry matter reduction in pigweed species was achieved with propane dose ranging from 40 to 80 kg ha−1, depending on the growth stage when flaming was conducted. A similar dose of 40 to 60 kg ha−1 provided 80% reduction in dry matter for both foxtail species when flaming was done at their vegetative growth stage. However, none of the doses we tested could provide 90% dry matter reduction in foxtail species at flowering stage. It is important to note that foxtail species started regrowing 2 to 3 wk after flaming. Broadcast flaming has potential for control or suppression of weeds in organic farming.

Greenhouse and laboratory experiments were conducted on common waterhemp and soil collected from 131 soybean fields in Missouri that contained late-season common waterhemp escapes. The objectives of these experiments were to determine the effects of soil sterilization on glyphosate-resistant (GR) and -susceptible (GS) common waterhemp survival, to determine the effects of soil sterilization and glyphosate treatment on infection of GR and GS common waterhemp biotypes by Fusarium spp., and to determine the soil microbial abundance and diversity in soils collected from soybean fields with differences in common waterhemp biotypes and herbicide and crop rotation histories. Common waterhemp biotypes were treated with 1.7 kg glyphosate ae ha−1 or left untreated once plants reached approximately 15 cm in height. Common waterhemp survival was visually assessed at 21 d after glyphosate treatment (21 DAT). To determine Fusarium infection frequency, a single intact common waterhemp root was harvested from each treatment at 0, 3, 7, 14, and 21 DAT and surface sterilized, and 10 to 15–mm common waterhemp root sections were plated on Komada culture medium. After 14 d incubation, fungal colonies were selected from colonized roots and maintained on potato dextrose agar medium amended with antibiotics before identification. Speciation of Fusarium isolates was conducted through microscopic examination of fungal characters and confirmed by sequencing and analysis of ribosomal DNA. Soil samples from 131 different collections were subjected to phospholipid fatty acid (PLFA) analysis and were conducted utilizing gas chromatography to determine the soil microbial community abundance and structure. Common waterhemp plants grown in sterile soils had the highest common waterhemp survival, regardless of biotype. After treatment with glyphosate, survival of GS common waterhemp grown in nonsterile soil was only 29% 21 DAT, whereas survival of GS common waterhemp grown in nonsterile soil was only 10%. Similarly, GR common waterhemp survival was reduced from 83 to 61% following treatment with glyphosate when grown in nonsterile compared to sterile soil. Fusarium spp. were recovered from only 12% of the assayed roots (223 treatments with Fusarium out of a total 1,920 treatments). The greatest occurrence of Fusarium root infection in both GR and GS common waterhemp occurred in nonsterile soils following a glyphosate treatment. Few differences in total PLFA were observed in field soil collected from locations with either GR or GS common waterhemp, and regardless of herbicide or crop history. This research supports previous findings that plant species are more sensitive to glyphosate in nonsterile than sterile soils and indicates glyphosate may predispose plants to soil-borne phytopathogens. This research also suggests that continuous use of glyphosate does not significantly affect soil microbial abundance or diversity.

The introduction of transgenic crops with resistance to 2,4-D will provide growers with new weed management options in soybean. Field and greenhouse experiments were conducted in 2010 and 2011 to determine the influence of weed height on compatibility of glufosinate and 2,4-D in resistant soybean. Results from these experiments indicate a significant increase in the rate of glufosinate or 2,4-D is needed when applied alone in order to achieve similar levels of weed control as lower rates of glufosinate and 2,4-D combinations. Mixtures of glufosinate and 2,4-D were more effective in controlling common waterhemp, Asiatic dayflower, and barnyardgrass than either herbicide alone, and with the magnitude of difference greater as the height of common waterhemp increased. Large crabgrass control decreased when glufosinate was applied with 2,4-D compared with glufosinate alone, with the magnitude of the reduction greater as weed height increased. Sequential application of glufosinate and 2,4-D eliminated the antagonism noted with tank mixtures. Soybean yields were also reduced by approximately 3% in response to 30 to 35–cm compared to 10 to 15–cm herbicide applications because of the combination of weed interference and reduced control. Overall, results from these experiments indicate that glufosinate plus 2,4-D combinations are likely to provide increased control of problematic weeds like Asiatic dayflower and common waterhemp while providing similar grass control as herbicide programs that contain glufosinate alone.

Field experiments were conducted at two locations in Missouri in 2012 and 2013 to evaluate herbicide programs in 4-hydroxyphenylpyruvate dioxygenase (HPPD)-inhibitor-resistant soybean, referred to as FG72 soybean, and their tolerance to four HPPD-inhibiting herbicides. At the Columbia location, PRE followed by (fb) POST and two-pass POST treatments provided 97% or greater control of all weeds except ivyleaf morningglory. At Moberly in 2012, PRE fb POST treatments provided 95% or greater control and 100% biomass reduction (BR) of glyphosate-resistant (GR) waterhemp, with the exception of isoxaflutole at 0.04 kg ha−1 plus S-metolachlor at 0.6 kg ha−1 plus metribuzin at 0.2 kg ha−1. In 2013, PRE fb POST treatments provided greater than 89% control and 93% BR. Two-pass POST treatments of isoxaflutole plus glyphosate always provided greater control and BR of GR waterhemp compared with glyphosate fb glyphosate. However, at Columbia, where glyphosate-susceptible weeds were present, there were no differences in control or BR between two-pass POST treatments. In the soybean tolerance experiment, isoxaflutole provided the lowest levels of injury. Applications of tembotrione at the 1× rate resulted in the greatest injury in both years. Topramezone at the 1× rate always provided less injury than tembotrione, but was always similar in BR. The 2× rates increased soybean injury over the 1× rate for the third trifoliate (V3) application, but not for the PRE and first-flower (R1) applications. V3 and R1 applications of isoxaflutole and mesotrione resulted in similar injury, height reduction, and BR to soybean 28 d after application in 2012 and 2013. Overall these results indicate that FG72 soybean could allow the use of HPPD-inhibiting herbicides such as mesotrione PRE along with isoxaflutole PRE and POST to provide an additional herbicide mechanism of action that was not previously available in soybean.

Glyphosate-resistant (GR) volunteer corn is a significant problem weed in soybean grown in rotation with corn in the midwestern United States and eastern Canada. The objective of this study was to evaluate the efficacy of glufosinate applied in single or sequential applications compared with acetyl-coenzyme A carboxylase (ACCase) inhibitors applied alone or tank mixed with glufosinate for controlling GR volunteer corn in glufosinate-resistant soybean. At 15 d after early-POST (DAEP), ACCase inhibitors applied alone controlled volunteer corn 76 to 93% compared to 71 to 82% control when tank mixed with glufosinate. The expected volunteer corn control achieved by tank mixing ACCase inhibitors and glufosinate was greater than the glufosinate alone, indicating that glufosinate antagonized ACCase inhibitors at 15 DAEP, but not at later rating dates. ACCase inhibitors applied alone or tank mixed with glufosinate followed by late-POST glufosinate application controlled volunteer corn and green foxtail ≥ 97% at 30 DAEP. Single early-POST application of glufosinate controlled common waterhemp and volunteer corn 53 to 78%, and green foxtail 72 to 93% at 15 DAEP. Single as well as sequential glufosinate applications controlled green foxtail and volunteer corn greater than or equal to 90%, and common waterhemp greater than 85% at 75 d after late-POST (DALP). Contrast analysis suggested that glufosinate applied sequentially provided greater control of volunteer corn at 15 and 75 DALP compared to a single application. Similar results were reflected in volunteer corn density and biomass at 75 DALP. Volunteer corn interference did not affect soybean yield, partly because of extreme weather conditions (hail and high winds) in both years of this study.

Field experiments were conducted in 2012 and 2013 to determine the effects of row spacing, seeding rate, and herbicide programs on multiple-resistant waterhemp control and yield in glufosinate-resistant soybean. The two herbicide programs evaluated were: (1) a PRE application of fomesafen plus S-metolachlor followed by early POST application of glufosinate plus acetochlor, referred to as the PRE followed by (fb) POST with residual (w/RES) herbicide program; and (2) an early POST followed by a late POST application of glufosinate, referred to as the two-pass POST herbicide program. Results indicate that the PRE fb POST w/RES program provides greater control of resistant waterhemp compared to the two-pass POST herbicide program. In 2012, the PRE fb POST w/RES program resulted in a 99% waterhemp density reduction and 156 kg ha−1 increase in yield compared to the 72% density reduction by the two-pass POST program. In 2013, the two-pass POST program was equally as effective on density reduction and yield as the PRE fb POST w/RES program. Waterhemp control and density reduction was always greatest with 19- and 38- compared to 76-cm rows. In 2012, the PRE fb POST w/RES program provided at least 95% control and greater than 98% density reduction across all row spacings, whereas the two-pass POST program provided 95%, 95%, and 85% control and 87%, 80%, and 50% density reduction in 19-, 38-, and 76-cm rows, respectively. Soybean seeding rate did not affect waterhemp control or density in either year. In both years, 165,000 seeds ha−1 yielded lower than the three higher seeding rates. Overall, results from these experiments indicates that the use of a PRE fb POST w/RES program, narrow-row spacing, and seeding rates of 240,000 to 315,000 seeds ha−1 or greater provides the greatest waterhemp control, density reduction, and soybean yield when multiple resistant waterhemp is present.

Field and greenhouse experiments were conducted to determine the level of glyphosate resistance in common waterhemp populations from Platte County (MO1) and Holt County, Missouri (MO2), and to determine the level and distribution of resistance to glyphosate, acetolactate synthase (ALS)–inhibiting herbicides, and protoporophyrinogen oxidase (PPO)–inhibiting herbicides across the MO1 site. Results from greenhouse experiments revealed that the MO1 and MO2 waterhemp populations were 19 and 9 times more resistant to glyphosate, respectively, than a susceptible waterhemp population. In field experiments, greater than 54% of waterhemp at the MO1 site survived 1.7 kg glyphosate ae ha−1 (twice the labeled rate) 6 wk after treatment. Tank-mix combinations of ALS- and PPO-inhibiting herbicides with glyphosate also failed to provide complete control of the waterhemp population at the MO1 site. Collection and screening of seed from individual female waterhemp accessions revealed multiple resistance to glyphosate, ALS-, and PPO-inhibiting herbicides across the MO1 site. All 14 waterhemp accessions collected across the MO1 site exhibited greater than 65% survival to 2× rates of glyphosate and thifensulfuron, and these accessions were spread across a 5-km2 (503-ha) area. Four waterhemp accessions collected across a 0.9-km2 (87-ha) area also exhibited 26 to 38% survival to 2× rates of lactofen. The results from these experiments provide evidence and confirmation of the first glyphosate-resistant waterhemp population in the United States and reveal that multiple resistance to glyphosate, ALS-, and PPO-inhibiting herbicides can occur in waterhemp.

Although the prolonged emergence pattern of common waterhemp is well known, its effect on flowering phenology and success is less well understood. The ecological significance of later common waterhemp cohorts could have been underestimated. Ecological knowledge of common waterhemp, such as reproductive phenology and success, would help us better understand the invasiveness of this weed and thus facilitate the development of more targeted control methods. Field studies were conducted at Ames, IA, to evaluate temporal variation in flowering phenology (e.g., date of flower initiation, number of flowering plants per day) and reproductive success (e.g., seed production) of natural common waterhemp cohorts. Later-emerging common waterhemp cohorts flowered quicker and had a relatively shorter flowering period than early cohorts. Common waterhemp cohorts showed very large variations for the duration of their vegetative phases, indicating different photoperiod sensitivity among cohorts. Furthermore, common waterhemp cohorts exhibited a pulsed flowering pattern, which was probably influenced by temporal distribution of rain events; up to seven distinct flowering pulses within 40 d were observed in 2009, and about eight flowering pulses scattered over a 60 d period were observed in 2010. Common waterhemp maintained high seed production throughout the growing season. Seed production for the entire cohort was influenced more by emergence timing, whereas individual plant fecundity was more affected by plant population densities. Common waterhemp demonstrates strong phenological plasticity by evolving a pulsed flowering pattern, which rendered it plastic enough to tailor flowering to variable environmental conditions thus facilitating effective pollination. Common waterhemp later cohorts have a strong seed production potential, which may be ecologically significant with regard to herbicide resistance evolution.

Glyphosate-resistant common waterhemp is a difficult-to-control annual broadleaf weed that has become a serious management challenge for growers in Nebraska and other states in the United States. The objectives of this study were to confirm glyphosate-resistant common waterhemp in Nebraska by quantifying level of resistance in a dose-response study, and to determine the sensitivity and efficacy of POST soybean herbicides for controlling suspected glyphosate-resistant common waterhemp biotypes. Seeds of suspected glyphosate-resistant common waterhemp biotypes were collected from seven eastern Nebraska counties. Greenhouse dose-response experiments were conducted to evaluate the response of common waterhemp biotypes to nine rates of glyphosate (0 to 16×). Common waterhemp biotypes were 3- to 39-fold resistant to glyphosate depending on the biotype being investigated and the susceptible biotype used for comparison. Results of the POST soybean herbicides efficacy experiment suggested that glyphosate-resistant biotypes, except a biotype from Pawnee County, had reduced sensitivity to acetolactate synthase (ALS)–inhibiting herbicides (chlorimuron-ethyl, imazamox, imazaquin, imazethapyr, and thifensulfuron-methyl). Glufosinate and protoporphyrinogen oxidase (PPO)–inhibiting herbicides (acifluorfen, fluthiacet-methyl, fomesafen, and lactofen) provided ≥ 80% control of glyphosate-resistant common waterhemp at 21 d after treatment (DAT). This study confirmed the first occurrence of glyphosate-resistant common waterhemp in Nebraska, and also revealed reduced sensitivity to ALS-inhibiting herbicides in most of the biotypes tested in this study.

The evolution of glyphosate and acetolactate synthase (ALS) inhibitor-resistant common waterhemp in the Midwestern United States has reduced the number of effective POST herbicide options for management of this problem weed in glyphosate-resistant soybean. Moreover, common waterhemp emerges throughout the crop growing season, justifying the need to evaluate herbicide programs that provide season-long control. The objectives of this study were to compare POST-only and PRE followed by (fb) POST herbicide programs for control of glyphosate-resistant common waterhemp in glyphosate-resistant soybean. Field experiments were conducted in 2013 and 2014 in Dodge County, NE, in a field infested with glyphosate-resistant common waterhemp. Programs containing PRE herbicides resulted in ≥83% control of common waterhemp and densities of ≤35 plantsm–2 at 21 d after PRE (DAPRE). Post-only herbicide programs resulted in <70% control and densities of 107 to 215 plants m–2 at 14 d after early-POST (DAEPOST) treatment. PRE fb POST herbicide programs, including saflufenacil plus imazethapyr plus dimethenamid-P, sulfentrazone plus cloransulam, or S-metolachlor plus metribuzin, fb fomesafen plus glyphosate; S-metolachlor plus fomesafen fb acifluorfen plus glyphosate resulted in >90% control of glyphosate-resistant common waterhemp throughout the growing season, reduced density to ≤7plantsm–2, ≥92% biomass reduction, and soybean yield >2,200kg ha–1. Averaged across herbicide programs, common waterhemp control was 84%, and density was 15 plants m–2 with PRE fb POST herbicide programs compared with 42% control, and density of 101 plants m–2 with POST-only herbicide programs at harvest. Results of this study indicated that PRE fb POST herbicide programs with effective modes of action exist for season-long control of glyphosate-resistant common waterhemp in glyphosate-resistant soybean.