Climate change is causing the geographical ranges of some species to track suitable conditions. Habitat specialists, range-restricted species and species with limited dispersal abilities may be unable to track changing conditions, increasing their extinction risk. In response to changing conditions and species movement patterns, there is a need to account for the effects of climate change when designing protected areas and identifying potential climate refugia. We used ecological niche models projected into future climates to identify potential impacts of climate change on the distribution of 11 rupicolous reptile species in the Soutpansberg Mountains, South Africa. Lygodactylus incognitus, Lygodactylus soutpansbergensis, Platysaurus relictus and Vhembelacerta rupicola were identified as being vulnerable to climate change due to substantial reductions in suitable habitat and low spatial overlap between current and future niche envelopes. We identified areas of high conservation importance for the persistence of these species under present-day and projected future conditions. The western Soutpansberg was identified as an area of high conservation priority as it is a potential refuge under future projections. Projecting distributions of vulnerable species into future climate predictions can guide future research and identify potential refugia that will best conserve species with restricted ranges in a world with climate change.

The species of the Parmelia saxatilis complex occurring in the Iberian Peninsula were revised. Eight species are accepted, including a new species found in southern Spain, described as P. rojoi A. Crespo, V. J. Rico & Divakar. The new species, which forms a sister-group relationship with P. saxatilis s. str., is rare in the Iberian Peninsula and is restricted to higher altitudes of northern and central Spain. Parmelia rojoi differs from P. saxatilis by generally narrower isidia and a more fragile thallus. The segregation of the new species is also supported by ITS (rDNA) and Mcm7 (MS456) phylogeny and multispecies coalescent-based approaches, including StarBEAST and BP&P. Furthermore, the divergence of P. rojoi is dated back to the Pleistocene, c. 2.13 Ma. A key to the identification of species from the P. saxatilis complex with their diagnostic features is provided. All species of the complex known from Europe are also found in the Iberian Peninsula. We hypothesize that P. rojoi is a relict species that survived the Pleistocene glaciations in refugia in Spain and has been unable to extend its distributional range in postglacial periods.

Mitochondrial and nuclear sequence data from two Antarctic ameronothroid mites, Halozetes belgicae and Alaskozetes antarcticus, were used to address three key questions important for understanding both the evolution of biodiversity and its future conservation in the Antarctic Peninsula Region: i) Do populations of mites across the Antarctic Peninsula and Scotia Arc constitute distinct genetic lineages? ii) What implications does the spatial genetic structure in these species have for current understanding of the region’s glacial history? iii) What are the conservation implications of these findings? Our results indicate that both mite species have been present in the Antarctic since at least the Pliocene. At the regional scale, both species are comprised of a number of divergent, but sympatric, lineages that are genetically as distinct as some species within the genera Halozetes and Alaskozetes. At the local scale, complex structure suggests limited and stochastic post-Holocene dispersal. For both species, considerable spatial genetic structure exists across the region, similar to that found in other terrestrial invertebrates. These results support the implementation of stringent biosecurity measures for moving between the Scotia Arc islands and the Antarctic Peninsula, and throughout the latter, to conserve both evolutionary history and future evolutionary trajectories.

Myriospora signyensis Purvis, Fdez-Brime, M. Westb. & Wedin is described from Signy Island, South Orkney Islands, Antarctica, where it occurs predominantly on quartz mica schist. This represents the first record of the genus for Antarctica. The distinctive interrupted photobiont arrangement places it within the genus Myriospora (formerly known as the ‘Acarospora’ smaragdula group, or Silobia). The new species is characterized by having large, distinctly elevated, sessile apothecia with a prominent margin and a thallus that is usually lobed at the margins and variously orange-red, rust-coloured or brown-pigmented. Molecular phylogenetic analyses inferred with strong support that M. signyensis is closely related to M. scabrida which is similar in having a lobed, imbricate thallus with large and frequently somewhat raised apothecia, but which differs in never being rusty red, by frequently having a larger number of apothecia per areole/squamule and by having a thick and distinctive thalline epinecral layer. Myriospora signyensis is otherwise most similar to M. dilatata but the thallus of M. dilatata is never imbricate-lobate and the ascomata of M. signyensis have larger and more distinctly raised and sessile apothecia. A worldwide key to the 10 species currently recognized in the genus is presented.

During the warmer Holocene Period, two major climatic crises affected the Central African rainforests. The first crisis, around 4000 cal yr BP, caused the contraction of the forest in favor of savanna expansion at its northern and southern periphery. The second crisis, around 2500 cal yr BP, resulted in major perturbation at the forest core, leading to forest disturbance and fragmentation with a rapid expansion of pioneer-type vegetation, and a marked erosional phase. The major driver of these two climatic crises appears to be rapid sea-surface temperature variations in the equatorial eastern Atlantic, which modified the regional atmospheric circulation. The change between ca. 2500 to 2000 cal yr BP led to a large increase in thunderstorm activity, which explains the phase of forest fragmentation. Ultimately, climatic data obtained recently show that the present-day major rise in thunderstorms and lightning activity in Central Africa could result from some kind of solar influence, and hence the phase of forest fragmentation between ca. 2500 to 2000 cal yr BP may provide a model for the present-day global warming-related environmental changes in this region.

Our study combines new geological and paleoecological information to reconstruct the glacial history and terrestrial paleoenvironments on Haida Gwaii during the advance phase of the Fraser glaciation (Marine Isotope Stage 2). At Cape Ball on eastern Graham Island, five accelerator mass spectrometry radiocarbon ages ranging from 23,200±280 to 26,650±390 14C yr BP (ca. 27,000–31,400 cal yr BP) record the earliest approach of mainland glaciers to Haida Gwaii. Abundant marine dinoflagellate cysts indicate isostatic depression by glacial ice in Hecate Strait to the east. At Mary Point on the north coast of Graham Island, similar outwash of a piedmont lobe advancing westward along Dixon Entrance preserves plant remains dated from 19,270±360 to 23,740±300 14C yr BP (22,500–28,600 cal yr BP). These sediments also contain marine indicators. Plant macrofossils, pollen, and invertebrates support the geological evidence of a proglacial environment under a colder-than-present macroclimate. Although some trees were likely present on Graham Island at this time, tundra-like plant communities dominated low-lying areas. A large area that appears to have been ice-free during this time is a portion of the continental shelf off the east coast of Moresby Island, referred to provisionally as the “Hecate Refugium.”

Recent changes along the margins of the Antarctic Peninsula, such as the collapse of the Wilkins Ice Shelf, have highlighted the effects of climatic warming on the Antarctic Peninsula Ice Sheet (APIS). However, such changes must be viewed in a long-term (millennial-scale) context if we are to understand their significance for future stability of the Antarctic ice sheets. To address this, we present nine new cosmogenic 10Be exposure ages from sites on NW Alexander Island and Rothschild Island (adjacent to the Wilkins Ice Shelf) that provide constraints on the timing of thinning of the Alexander Island ice cap since the last glacial maximum. All but one of the 10Be ages are in the range 10.2–21.7 ka, showing a general trend of progressive ice-sheet thinning since at least 22 ka until 10 ka. The data also provide a minimum estimate (490 m) for ice-cap thickness on NW Alexander Island at the last glacial maximum. Cosmogenic 3He ages from a rare occurrence of mantle xenoliths on Rothschild Island yield variable ages up to 46 ka, probably reflecting exhumation by periglacial processes.

Late glacial scenarios of ice retreat and biogeography databases constrain the dispersal routes of obligate freshwater fishes into Atlantic Canada and Maine. Evidence indicates glacial ice covered the present-day mainland and offshore islands at 18,000 14C yr before present. Possible refugia for extirpated freshwater fishes were the exposed outer edge of the Grand Banks (east), exposed Georges Bank (south-Atlantic Refugium), and the Mississippi Refugium in the west. It is improbable that the region was recolonized from the offshore refugia. Rather, fishes recolonized from the east via the upper St. Lawrence River valley into the upper Saint John River, Maine (Lake Madawaska) from 11,000 to 12,000 14C yr BP. The short period of entry resulted in the low diversity of obligate freshwater species in the region. Lake Madawaska was breached and dispersal continued into the remainder of the region after 8000 14C yr BP. By 6000 14C yr BP, access routes to the east along low-lying coastal zones were blocked by rising sea levels, which isolated Prince Edward Island, Cape Breton Island, and most probably Nova Scotia. Natural dispersal across the region appeared complete by this time.

Carbon isotopes of soil organic matter (SOM) were used to evaluate and establish the chronology of the vegetation dynamics of an ecosystem presently composed of savannas surrounded by forests. The study was carried out on a 200-km transect along highway BR 319, on the border of Amazonas and Rondônia states, in southern Amazon, Brazil. Large ranges in δ13C values were observed in SOM collected from profiles in the savanna (−27 to −14‰) and forest regions (−26 to −19‰), reflecting changing distribution of 13C-depleted C3 forest and 13C-enriched C4 savanna vegetation in response to climate change. These results indicate that from about 17,000 to 9000 14C yr B.P., the study area was covered by forest vegetation. Between approximately 9000 and 3000 14C yr B.P., savanna vegetation expanded at the expense of the forest. Although the expansion of savanna did not occur with the same intensity along the study transect, this process was very clearly registered by 13C-enrichment in the SOM. Since 3000 14C yr B.P., the carbon isotope data suggest that forested regions have expanded. This study adds to the mounting evidence that extensive forested areas existed in the Amazon during the last glaciation and that savanna vegetation expanded in response to warm and dry conditions during the early to middle Holocene.

Prevention of heartworm disease caused by Dirofilaria immitis in domestic dogs and cats relies on a single drug class, the macrocyclic lactones (MLs). Recently, it has been demonstrated that ML-resistant D. immitis are circulating in the Mississippi Delta region of the USA, but the prevalence and impact of these resistant parasites remains unknown. We review published studies that demonstrated resistance in D.immitis, along with our current understanding of its mechanisms. Efforts to develop in vitro tests for resistance have not yet yielded a suitable assay, so testing infected animals for microfilariae that persist in the face of ML treatment may be the best current option. Since the vast majority of D. immitis populations continue to be drug-sensitive, protected dogs are likely to be infected with only a few parasites and experience relatively mild disease. In cats, infection with small numbers of worms can cause severe disease and so the clinical consequences of drug resistance may be more severe. Since melarsomine dihydrochloride, the drug used to remove adult worms, is not an ML, the ML-resistance should have no impact on our ability to treat diseased animals. A large refugium of heartworms that are not exposed to drugs exists in unprotected dogs and in wild canids, which may limit the development and spread of resistance alleles.

Phylogeographic inferences concerning marine species are largely based on intertidal species. In high latitudes, intertidal species have been affected by ice coverage and ice scour, and therefore show northern range limitations during glaciations. In this study, we use the subtidal horse mussel (Modiolus modiolus) to investigate whether generalizations about genetic structure of high latititude intertidal species, specifically in the North Atlantic, are representative of other near shore taxa. We analysed genetic diversity, molecular variance, and geographical patterns of genetic relatedness using data from the mtDNA CO1 gene. Although we do find little to no haplotype structure in the North Atlantic, our results show that north-eastern Pacific individuals represent a different haplotype network with no haplotypes in common with Atlantic individuals. Thus, M. modiolus in the Pacific may represent an unrecognized species. Genetic diversity and population expansion times suggest a Pacific origin is most likely, with subsequent dispersal to the Atlantic. The lack of genetic structure in the Atlantic suggests that a rapid range expansion occurred less than 50 KYA, rather than a stepping stone mode of dispersal.

Refugia-based treatment strategies aim to prolong anthelmintic efficacy by maintaining a parasite population unexposed to anthelmintics. Targeted selective treatment (TST) achieves this by treating only animals that will benefit most from treatment, using a determinant criterion (DC). We developed a mathematical model to compare various traits proposed as DC, and investigate impacts of TST and drenching frequency on sheep performance and anthelmintic resistance. Short term, decreasing the proportion of animals drenched reduced benefits of anthelmintic treatment, assessed by empty body weight (EBW), but decreased the rate of anthelmintic resistance development; each consecutive drenching had a reduced impact on average EBW and an increased impact on the rate of anthelmintic resistance emergences. The optimal DC was fecal egg count, maintaining the highest average EBW when reducing the proportion of animals drenched. Long-term, reducing the proportion of animals drenched had little impact on total weight gain benefits, across animals and years, whilst reducing drenching frequency increased it. Decreasing the frequency and proportion of animals drenched were both predicted to increase the duration of anthelmintic efficacy but reduce the total number of drenches administered before resistance was observed. TST and frequency of drenching may lead to different benefits in the short versus long term.

Many mires of high conservation value occur in Bulgaria. These mires acted as refugia during the dry phases of ice ages and therefore contain a high number of rare and disjunct species. The mires harbour specific ecotypes and genotypes of plants and animals, and thus provide an opportunity to test biogeographical hypotheses, and they also contain important information about the history of European mires. In this study we ranked all known mire and spring complexes in Bulgaria according to the occurrence of rare and threatened plant and mollusc species. This analysis shows a conspicuous concentration of rare species at several sites, and no correspondence between the importance of individual mires for biodiversity and their legal protection. Of the 10 mire complexes of highest priority only one is effectively protected. The remaining unprotected mires have either been destroyed or are threatened by ongoing development. Having joined the European Union, Bulgaria has built a Natura 2000 network that could provide an opportunity for mire conservation. However, destruction of mire habitats proceeds faster than the approval of Natura 2000 sites. There is thus a possibility that unique Bulgarian mires will be lost before the Natura 2000 system begins to perform its role. Only effective and timely protection of the mire remnants, together with appropriate management, will ensure the future of these unique habitats.

The herpetofauna of a floodplain monsoon rain forest in northern Australia is composed primarily of species from non rain forest habitats. The majority of frog species use rain forest as a seasonal refuge, and there is a marked increase in numbers during the dry season. Faunal richness lies within limits expected on the basis of the length of the dry season and species richnesses of non-Australian faunas. There are few lizard species and an abundance of frog species (none of which is a rain forest specialist) in comparison to rain forest herpetofaunas in other tropical regions. The impoverished lizard fauna, and the paucity of rain forest specialists may be because (a) seasonal invasion of rain forest by frogs prevents evolution of, or colonization by, specialists or (b) rain forest specialists may not have been able to cross semiarid habitats separating the Northern Territory from eastern Australian rain forests. The herpetofaunas of monsoon forests in Cape York Peninsula may provide a means of distinguishing between these hypotheses.

Integrated pest management strategies for cereal processing facilities often include both pheromone-baited pitfall traps and crack and crevice applications of a residual insecticide such as the pyrethroid cyfluthrin. In replicated pilot-scale warehouses, a 15-week-long experiment was conducted comparing population trends suggested by insect captures in pheromone-baited traps to direct estimates obtained by sampling the food patches in untreated and cyfluthrin-treated warehouses. Warehouses were treated, provisioned with food patches and then infested with all life stages of Tribolium castaneum (Herbst). Food patches, both those initially infested and additional uninfested, were surrounded by cyfluthrin bands to evaluate if insects would cross the bands. Results show that insect captures correlated with population trends determined by direct product samples in the untreated warehouses, but not the cyfluthrin-treated warehouses. However, dead insects recovered from the floor correlated with the insect densities observed with direct samples in the cyfluthrin-treated warehouses. Initially, uninfested food patches were exploited immediately and after six weeks harbored similar infestation densities to the initially infested food patches. These data show that pest management professionals relying on insect captures in pheromone-baited traps in cyfluthrin-treated structures could be deceived into believing that a residual insecticide application was suppressing population growth, when the population was actually increasing at the same rate as an untreated population.

The evolutionary relationships of several, mainly Iberian, Jasione taxa (Campanulaceae) were investigated using molecular data. A parsimony analysis of the internal transcribed spacer (ITS) region of nuclear ribosomal DNA was partially successful in elucidating some interspecific alliances. One of the main clades included three morphologically rather disparate species from S Spain and Morocco which might indicate that these areas were refugia during the last glaciations. The fact that most of the other taxa fell in a large polytomy suggests a recent origin of these species. Amplified fragment length polymorphisms (AFLPs) were analysed for a larger sample set of two taxa from within the ITS polytomy to assess the partition of genetic variation between and within populations. All the British accessions named J. montana var. montana were found to be close to those so-named from Spain. However, individual accessions of J. montana var. montana from Argyll (Scotland), Devon (SW England) and the Shetland Islands proved to be genetically different from each other. This might suggest a low level of genetic diversification from a common progenitor due to a rapid northward migration. It is proposed that J. montana var. bracteosa and J. maritima var. sabularia merit recognition at higher rank. The possibility that J. montana vars gracilis and latifolia are mere repetitive segregants of J. montana var. montana is considered.

Transgenic plants expressing Bacillus thuringiensis δ-endotoxins are now being used commercially in several crop species. These toxins have demonstrated good control of temperate (Ostrinia nubilalis) and tropical (Diatraea grandiosella and D. saccharalis) stemborers in maize. Resistance to B. thuringiensis toxins has been reported in over 11 species in both field and laboratory studies, demonstrating the need for resistance management strategies to prolong the efficacy of this valuable pest management tool within an integrated control programme. Resistance involves reduced binding of toxins to midgut epithelial cells and is generally considered to be a recessive trait. Resistance management will require the use of spatial and temporal refugia which may require unique schemes for each pest complex. Information is presented on the mode of action of cry toxins, resistance mechanisms, interaction of transgenic plants and biocontrol agents, and management/deployment strategies for transgenic maize in tropical ecologies.

Dormancy is described for adults of the stalk-eyed fly, Diopsis longicornis Macquart (Diopsidae: Diptera) in the tropical savanna of coastal Guinée, West Africa. Populations of diopsids maintained themselves within each chosen refugium throughout the dry season unless the site dried up. Sex ratio within the populations at each refugium remained constant throughout the dry period. Slightly more females than males were found (56%), and of these only 0.8% were gravid. Movement of stalk-eyed fly adults from the rice field to the refugia corresponded with the end of the rice cropping period (stage of ripening and harvest) at the end of the wet season (late October to November), whereas movement from the refugia to the rice field occurred towards the end of the dry season Gate (May/early June), but well in advance of the rice crop planting (July and August) of the following wet season. Insects retained their state of reproductive arrest in the dry season even when offered suitable oviposition sites, i.e. rice plants. Secondary host plants were investigated as possible oviposition sites and food sources for adult flies.