Sulfentrazone was recently granted food-use tolerance approval for use on Brassica head and stem, as well as Brassica leafy vegetables. To date, one sulfentrazone registrant has listed those crops on its use label. In coastal California multiple crops per year including Brassica vegetables are grown in rapid succession; therefore, to avoid injury to rotational crops, herbicides used in those fields must be carefully selected. Given concerns about the relatively long soil persistence of sulfentrazone, studies were conducted to measure the response of direct-seeded carrot, lettuce, onion, spinach, and seeded tomato planted 3, 6, 9, and 12 mo after sulfentrazone application at 0, 112, 224, and 336 g ai ha−1. Eight plant-back studies were conducted during 2010–11 and 2012–13. Data collected were injury estimates, and stand and dry weights. Results indicate that it is safe to plant carrot and tomato 3 mo after sulfentrazone application at rates up to 336 g ai ha−1. Lettuce and green onion should not be planted within 9 mo of sulfentrazone application. Spinach should not be planted within 12 mo of sulfentrazone application.

Organic no-till (NT) management strategies generally employ high-residue cover crops that act as weed-suppressing mulch. In temperate, humid regions such as the mid-Atlantic USA, high-residue winter cover crops can hinder early spring field work and immobilize nutrients for cash crops. This makes the integration of cover crops into rotations difficult for farmers, who traditionally rely on tillage to prepare seedbeds for early spring vegetables. Our objectives were to address two separate but related goals of reducing tillage and integrating winter cover crops into early spring vegetable rotations by investigating the feasibility of NT seeding spinach (Spinacia oleracea L.), an early spring vegetable, into winterkilled cover crops. We conducted a four site-year field study in the Piedmont and Coastal Plain regions of Maryland, USA, comparing seedbed conditions and spinach performance after forage radish (FR) (Raphanus sativus L.), a low-residue, winterkilled cover crop, spring oat (Avena sativa L.), the traditional winterkilled cover crop in the area, a mixture of radish and oat, and a no cover crop (NC) treatment. NT seeded spinach after FR had higher yields than all other cover crop and tillage treatments in one site year and was equal to the highest yielding treatments in two site years. Yield for NT spinach after FR was as high as 19 Mg ha−1 fresh weight, whereas the highest yield for spinach seeded into a rototilled seedbed after NC was 10 Mg ha−1. NT seeding spring spinach after a winterkilled radish cover crop is feasible and provides an alternative to both high-residue cover crops and spring tillage.

To assess the genetic diversity of spinach germplasm, 250 individuals of 50 accessions collected from geographically diverse regions (West Asia, East Asia, Japan, Europe and the USA) were analysed using simple sequence repeat (SSR) markers. A total of 39 polymorphic alleles were identified, with an average of 6.5 alleles per locus for six loci. The overall gene diversity (0.62) in the entire set of individuals suggests that the germplasm has high genetic variability. The West Asian accessions showed the highest gene diversity, with a value of 0.57, followed by the East Asian accessions. These results help confirm the notion that spinach originated from West Asia. Hierarchical analysis of molecular variance revealed significant genetic differentiation among the geographical regions, which accounts for 26% of the total variation detected. Furthermore, pairwise Φst values indicate low genetic differentiation between the East Asian and Japanese germplasm accessions, both of which showed high genetic differentiation from the European accessions. The differentiation between the East Asian and European gene pools may be attributed to the founder effect associated with crop dissemination, as well as to the selection and genetic drift that occurred during the breeding process.

Spinach (Spinacia oleracea L.) seed quality is important for subsequent establishment of baby leaf and other spinach products. The indeterminate flowering pattern in spinach produces seeds of different sizes, and consequently a non-uniform seed lot. Sorting seeds based on novel sorting methods might therefore improve the establishment of spinach for producers. Spinach seeds were harvested at five different times (H1, H2, H3, H4 and H5) starting 3 weeks before estimated optimum harvest time. The harvested seeds were sorted according to chlorophyll fluorescence (CF) and seed size. Two harvest times 6 d apart were selected for further studies of seed development and germination counts. Analysis of mean germination time (MGT) of four germination counts (3, 7, 14 and 21 d) showed that smaller seed sizes ( < 3.25 mm) have lower MGT than seeds of larger sizes (>3.25 mm). A larger proportion of 2.5–3.25 mm size seeds had germinated on day 3 than both their larger and smaller counterparts at the later time of harvest (H4). Seeds with a diameter below 2.5 mm displayed the lowest MGT. Commercially, the use of chlorophyll fluorescence (CF)-sorted seeds, in combination with seed size sorting, may provide a useful tool for enhancing seed quality. Here our results demonstrate that a greater number of seeds with a low CF level had germinated on day 3 compared to the seeds with high CF levels. It is of relevance to develop a technology that could identify seeds that are sufficiently developed to germinate and successfully establish in the field regardless of seed size.

A greenhouse pot culture experiment was conducted to study the effect of graded levels ofwaste mica (0, 10, 20 and 40 g kg-1) on reducing the radiocesium uptake byspinach (Spinacia olerecea L) and lettuce (Lactuca sativaL.) grown in 134Cs-contaminated (at 37 k Bq kg-1 soil)Inceptisols, Vertisols and Ultisols. The biomass yield, and potassium content and itsuptake by crops have been significantly improved by waste mica application. The cropsgrown in Vertisols recorded higher biomass yield, and K content and its uptake as comparedwith Inceptisols and Ultisols. The average 134Cs transfer factor valuesrecorded were : 0.21, 0.17 and 0.26 at the first cutting, 0.15, 0.12 and 0.28 at thesecond cutting and 0.07, 0.05 and 0.23 at the third cutting from Inceptisols, Vertisolsand Ultisols, respectively. Waste mica significantly suppressed radiocesium uptake, theeffect being more pronounced at 40 g mica kg-1soil. There exists an inverserelationship between the 134Cs transfer factors with plant potassium contentand also the K uptake by the crops

The aim of the present study was to evaluate Mg absorption from a test meal served with an oxalate-rich vegetable, spinach, as compared with a test meal served with a vegetable with a low oxalate content, kale. Mg absorption was measured by a stable-isotope technique based on extrinsic labelling of the test meals and faecal monitoring of the excreted isotope labels. Nine healthy adults participated in the study. The test meals were based on 100g phytate-free white bread, served with 300g spinach (6·6mmol oxalate; 0·7mmol 25Mg label added, 5·0mmol total Mg) or 300g kale (0·1mmol oxalate; 1·2mmol 26Mg label added, 4·8mmol total Mg). The test meals were served on days 1 and 3, at breakfast and lunch, using a cross-over design. The results from the present study demonstrated that apparent Mg absorption was significantly lower from the meal served with spinach (26·7 (sd 10·4) %) than the meal served with kale (36·5 (sd 11·8) %) (P=0·01). However, the lower fractional apparent Mg absorption from the test meal served with spinach can be assumed to be, at least partly, counterbalanced by the higher native Mg content of spinach as compared with kale. Although based on indirect evidence, i.e. not based on an evaluation of added (or removed) oxalic acid, the difference in Mg absorption observed in the present study is attributed to the difference in oxalic acid content between the two vegetables.

Our knowledge on the absorption of folate is incomplete. The deconjugation process as a possible limiting factor in the absorption of folates was investigated. The study also attempted to validate the use of the area under the serum response curve (AUC) from food compared with folic acid as a proxy variable for food folate bioavailability. Folate absorption was determined in healthy ileostomy volunteers (n 11) using a single-dose short-term protocol. In a randomised crossover design, volunteers received spinach meals and a supplement. Based on analysis of test meals and ileostomy effluents, there was no difference in folate absorption between spinach with a mono-:polyglutamate ratio 40:60 and the same spinach with a 100:0 ratio. The absolute absorption of spinach folate (79 %) calculated from the difference between folate intake and folate content of ileostomy effluents was approximately equal to the relative absorption (81 %) calculated from the AUC after consumption of spinach meals in relation to the AUC after consumption of the folic acid supplement. We conclude that the deconjugation process is not a limiting factor in the absorption of spinach folates. Comparison of AUC of food folate v. folic acid in a short-term protocol may be suitable for assessing food folate bioavailability.