Adsorption and catalytic decomposition of 4-nitrobenzenesulphonylmethylcarbamate (herbicide Nisulam) on Upton, Wyoming, bentonite saturated with different cations was studied using thin-layer chromatography and infrared spectroscopy. Nisulam is adsorbed at room temperature by coordination through the NO2 group to the exchange cation regardless of the cation's nature. On moderate heating (75°–90°C) this molecule decomposes to 4-nitrobenzenesulphonamide whereas a similar compound (herbicide Asulam) containing the NH2 functional group instead of NO2 is adsorbed by protonation at room temperature and decomposes into different products. For cations having a high polarizing power, a coordination bond between the Asulam molecule's C=O group and the exchange cation is established, and the molecule decomposes to sulphanilic and carbamic acid. In contrast, for cations having a low polarizing power there is no coordination, and the molecule decomposes mainly into sulphanilamide. Nisulam's coordination to the exchange cation through the NO2 group instead of C=O is ascribed to inductive and conjugation effects, typical of the nitro group.

Sugarcane growers in Florida have been reporting reduced control of fall panicum with asulam, the main herbicide used for POST grass control. Therefore, outside container experiments were conducted to determine the response of four fall panicum populations from Florida to asulam applied alone and to evaluate whether tank-mix combination with trifloxysulfuron enhances control. Asulam was applied at 230 to 7,400 g ai ha−1, corresponding to 1/16 to 2X the maximum labeled rate for a single application in sugarcane, with or without combination with trifloxysulfuron at 16 g ai ha−1. Three fall panicum populations were collected from fields in which reduced control had been reported, while one population was from a field not used for sugarcane production but adjacent to a sugarcane field. The potency of asulam based on ED50 values (the rate required to cause 50% dry weight reduction at 28 d after treatment) ranged from 2,249 to 5,412 g ha−1 for tolerant populations with reported reduced fall panicum control compared with 1,808 g ha−1 for the susceptible population from the field not used for sugarcane production, showing that the latter was most sensitive to asulam. Addition of trifloxysulfuron to asulam increased potency on fall panicum by 5- to 15-fold, indicating that the tank mix enhanced dry weight reduction for all populations. The probability of fall panicum survival (regrowth after aboveground biomass harvesting) at the labeled rate of asulam ranged from 2% to 47% compared with 0% to 6% when trifloxysulfuron was added to the tank mix. Our results show differential response of fall panicum populations in Florida to asulam, which can be overcome by tank mixing with trifloxysulfuron even for populations that are difficult to control in sugarcane, but no evolution of resistance to asulam.

Amitrole (3-amino-s-triazole), asulam (methyl sulfanilylcarbamate), glyphosate [N-(phosphonomethyl)glycine], and fosamine [ethyl hydrogen (aminocarbonyl)phosphonate] were effective in reducing the amount of shoot regrowth from field horsetail (Equisetum arvense L.) 3 months after treatment. The order of effectiveness was amitrole > glyphosate > asulam > fosamine. The advantage of adding surfactant to the spray formulation or applying herbicides to wet foliage was rate dependent for all herbicides except fosamine. Spray retention was increased by application to wet foliage and by the inclusion of surfactant in the formulation. Spray retention increased as spray volume and plant size increased and as plant growth became prostrate. A scanning electron microscope study of the shoot surfaces showed an abundance of silica-containing structures and the presence of epicuticular wax. Certain surfaces of the scale leaves did not have these characteristics, indicating that redistribution of spray to these areas may result in greater penetration with subsequent increases in herbicide performance.

Preemergence control of itchgrass in sugarcane with nonincorporated pendimethalin and prodiamine increased as rate for each herbicide increased from 2.2 to 3.4 kg ai ha-1. Itchgrass control at 3.4 kg ha-1 was similar for both herbicides, ranging from 78 to 94% (86% mean) for pendimethalin in six field experiments and 83 to 91% (86% mean) for prodiamine in three experiments. Incorporated trifluralin at 2.2 kg ha-1, the standard, gave 99% mean control of itchgrass. None of the nonincorporated treatments with pendimethalin or prodiamine consistently prevented a decrease in the sugarcane stalk population at harvest. Asulam applied postemergence at 3.7 kg ae ha-1 controlled itchgrass that survived the preemergence herbicides, and sugarcane stalk populations were maintained. Preemergence control of johnsongrass with nonincorporated treatments of pendimethalin or prodiamine at 2.8 kg ha-1, in a mixture with atrazine at 2.2 kg ai ha-1, ranged from 86 to 95% (89% mean) for pendimethalin and 80 to 95% (88% mean) for prodiamine. Metribuzin at 2.2 kg ha-1, the standard, gave 97% mean control of johnsongrass. Mixtures of pendimethalin with metribuzin or terbacil generally gave more effective control of johnsongrass than mixtures with atrazine. When used in conjunction with other herbicides, nonincorporated pendimethalin and prodiamine were effective alternatives to soil-incorporated treatments for weed control in sugarcane.

In glasshouse experiments, Panicum repens rhizomes with more than two nodes emerged from soil depths of 1 to 20 cm. Emergence from one-node rhizomes was greatly reduced at depths more than 5 cm. In contrast, culm emergence from ginger like rhizomes decreased when burial depth (BD) was greater than 20 to 30 cm. P. repens emerged from deeper soil levels with an increasing number of nodes per cutting. Culms emerged from 36% of total underground regenerative organs. About 75 and 95% of the emerged culms were recorded 30 and 60 d after burial, respectively, and 91% emergence was recorded from 20-cm BD. In field studies, only 9% of nodes produced culms following cross-plowing. About 61 and 67% of the emerged culms were recorded 50 and 60 d after land preparation, respectively. Results indicate that mechanical and chemical control methods could be most effective for P. repens when applied approximately 50 d after land preparation. Burying deeper than 30 cm by deep plowing and reducing rhizome length by cross-plowing might effectively reduce emergence of P. repens.

Seedling alfalfa was injured by chlorsulfuron and sethoxydim plus 2,4-DB but recovered the following year as alfalfa seed yields were comparable to the hand-weeded control. Fluazifopbutyl and flamprop-methyl (active isomer) at 0.5 kg/ha did not affect seedling alfalfa adversely; however, seed yields were lower the following year compared to those treated at the 0.25-kg/ha rate. In established alfalfa, sethoxydim alone or with 2,4-DB applied annually did not affect seed yields adversely. However, 2,4-DB alone reduced seed yields in 2 of 5 yr. Alfalfa tolerated chlorsulfuron at 0.015 kg/ha but was damaged at the 0.03-kg/ha rate. Initial injury and later recovery without adverse effects on seed yield also were observed following annual treatments with bromoxynil, MCPA plus 2,4-DB and bentazon plus 2,4-DB.

Sugarcane (Saccharum officinarum L.) was planted in the fall; the seed cane was healthy or infected either with sugarcane mosaic virus (strain H) or with ratoon stunting disease (RSD) (Clavibacter xyli). Herbicide treatments were applied the following spring and each subsequent spring during the 3-yr crop cycle. Hexazinone [3-cyclohexyl-6-(dimethylamino)-1-methyl-1,3,5-triazine-2,4(1H,3H)-dione] at 1.1 kg ai/ha was applied as a soil foliage treatment when sugarcane was about 30 cm tall. The methyl ester of diclofop {(±)-2-[4-(2,4-dichlorophenoxy)phenoxy] propanoic acid} at 1.1 kg ai/ha and the sodium salt of asulam {methyl [(4-aminophenyl)sulfonyl] carbamate} at 3.4 or 3.8 kg ai/ha were applied as foliage treatments when sugarcane was about 90 cm tall. Both mosaic and RSD reduced yield of sugar/ha by 16% for the 3-yr crop, primarily by causing a decrease in stalk population. Hexazinone reduced yield of sugar/ha by 24% primarily by causing a decrease in both stalk population and sugar content of stalks. Diclofop reduced yield by 7% and asulam caused no significant yield loss. Combinations of disease and herbicide treatment reduced yields in an additive manner, with the combination of hexazinone and either mosaic or RSD causing a 38% reduction.

Response of wild oat (Avena fatua L.) at the three-leaf stage to five postemergence herbicides, asulam (methyl sulfanilylcarbamate), barban (4-chloro-2-butynyl m-chlorocarbanilate), diclofop {2-[4-(2,4-dichlorophenoxy)phenoxy] propanoic acid}, difenzoquat (1,2-dimethyl-3,5-diphenyl-1 H-pyrazolium), and flamprop [N-benzoyl-N-(3-chloro-4-fluorophenyl)-DL-alanine] was measured and compared in nine physiological and biochemical processes (chlorophyll content, photosynthesis, photosynthate translocation, and changes in ATP, lipids, DNA, RNA, amino acids, and proteins). Content of chlorophyll a and b was reduced by the application of diclofop and difenzoquat. Photosynthesis was inhibited with all five herbicides, but photosynthate translocation from the leaves to the roots was reduced only with diclofop, difenzoquat, and flamprop. ATP production was inhibited by all herbicides except flamprop, which increased the ATP content. Incorporation of 32P into lipids was reduced by all herbicides except asulam. The incorporation of 32P into nucleic acids (DNA and RNA) was inhibited by all five herbicides. Serine and threonine, especially the latter, accumulated in large quantity with all herbicides except difenzoquat. Protein content (water-soluble,-insoluble, and total amount) was reduced by asulam, diclofop, and flamprop, but barban and difenzoquat reduced water-insoluble and total proteins only. Apparently, all herbicides examined affected more than one physiological function and biochemical process in wild oat.

Applications of 2,4-D [(2,4-dichlorophenoxy)acetic acid] at 1.1 kg/ha at the start of each growing season severely damaged established alfalfa (Medicago sativa L. ‘Beaver’) and reduced seed yield 32% over a 4-yr period. Dandelion (Taraxacum officinale Weber) populations were reduced but perennial sowthistle (Sonchus arvensis L.) increased in frequency. Dichlobenil (2,6-dichlorobenzonitrile) applied at 2.1 kg/ha controlled both dandelion and perennial sowthistle. Alfalfa was damaged initially but rapid recovery resulted in an increase in seed yield in 2 yr out of 4. Terbacil (3-tert-butyl-5-chloro-6-methyluracil) applied at 1.1 kg/ha also controlled dandelion and perennial sowthistle. No alfalfa damage was evident and seed yields were increased 34% over a 3-yr period. Seed yield increased 30% where asulam (methyl sulfanilylcarbamate) was applied at 4.5 kg/ha, but there was no visible effect on the growth of either alfalfa or weeds. Simazine [2-chloro-4,6-bis (ethylamino)-s-triazine], propham (isopropyl carbanilate) + PCMC (p-chlorophenyl-N-methyl carbamate), pronamide [3,5-dichloro(N-1,1-dimethyl-2-propynyl)benzamide], and diuron [3-(3,4-dichlorophenyl)-1,1-dimethylurea], did not affect alfalfa seed production significantly, although simazine and diuron did reduce dandelion populations.

Carpetgrass is a warm season grass used in the south as low maintenance turf, especially in wet areas where other turf species do not persist. The response of carpetgrass to postemergence (POST) herbicides currently available for turf producers has not been thoroughly investigated and was the objective of four experiments conducted over a 2-yr period. Slight reduction in turf quality followed application of atrazine (2.2 kg ai ha-1), bentazon (2.2 kg ai ha-1), imazaquin (0.4 and 0.6 kg ai ha-1), mecoprop (1.1 kg ai ha-1), triclopyr (0.6 kg ai ha-1), metsulfuron (0.2 kg ai ha-1), 2,4-D (1.1 kg ai ha-1), 2,4-D plus dicamba (0.8 + 0.3 kg ai ha-1), and 2,4-D plus dicamba plus mecoprop (0.9 + 0.1 + 0.5 kg ai ha-1). Marginally acceptable turf quality followed sulfometuron (0.2 kg ai ha-1), sethoxydim (0.3 and 0.6 kg ai ha-1), and diclofop (2.2 kg ai ha-1) treatments. Unacceptable turf quality resulted from asulam (2.2 kg ai ha-1) and MSMA (1.1 and 2.2 kg ai ha-1) treatments. Carpetgrass tolerated most POST broadleaf and sedge herbicides but was sensitive to most POST grass herbicides used in other turf species, with the exception of sethoxydim.

Old World climbing fern (OWCF) is a highly invasive fern that disrupts natural communities in central and southern Florida. OCWF produces copious, wind-blown spores that have propelled its rapid invasion of Florida's natural areas over the last few decades. Current management of OWCF is limited to herbicides and natural resource managers in Florida have questioned if herbicides inhibit spore germination. This study compared spore germination rates of OWCF exposed to six herbicides and a surfactant from 1 to 24 h under laboratory conditions. Spores of OWCF were highly susceptible to metsulfuron, but exhibited tolerance to imazapyr, glyphosate, fluroxypyr, asulam, and triclopyr. Spore germination rates were 0.4% for spores exposed to 0.1 g ai L−1 of metsulfuron, but 0% for rates greater than or equal to 0.2 g ai L−1 at 30 d after treatment (DAT). Reduction in spore germination was observed with all other concentrations of herbicides tested, ranging from 10.4% with triclopyr (40 g ai L−1) to 42.6% with asulam (4.2 g ai L−1) compared to 47.9% germination for untreated checks 30 DAT. Spores were highly sensitive to metsulfuron with herbicide concentrations required for 50 and 95% inhibition of spore germination (I50 and I95) measuring 0.014 and 0.063 g ai L−1, respectively; spores were greater than 1,000-fold more sensitive to metsulfuron compared to I95 concentrations of any other herbicide tested. These results indicate that metsulfuron exhibits potential to control OWCF spore germination but spores are tolerant to the five other herbicides tested.

It has been suggested that trifloxysulfuron might increase the efficacy of asulam for control of johnsongrass. Container and field studies were conducted to determine the efficacy of POST applications of trifloxysulfuron and asulam for johnsongrass control in sugarcane. Asulam was applied at 460 and 920 g ai/ha to container-grown johnsongrass plants, with and without 8 g ai/ha of trifloxysulfuron. Combinations of asulam and trifloxysulfuron generally reduced johnsongrass height, rhizome length, and biomass more than when either was applied alone. Results suggested that combinations of asulam and trifloxysulfuron were synergistic in their control of johnsongrass biomass 8 wk after treatment. In a sugarcane field heavily infested with rhizome johnsongrass, asulam was applied at 1,800, 2,800, and 3,700 g/ha with and without trifloxysulfuron at 16 g/ha. Asulam plus trifloxysulfuron generally controlled johnsongrass more effectively than either herbicide alone. The control of johnsongrass with asulam at 1,800 g/ha resulted in an increase in sugar yield of more than twice that in the nontreated control. Sugar yield increased further when asulam was applied at 2,800 g/ha or combined with trifloxysulfuron, but application of trifloxysulfuron alone did not increase yield. Combinations of asulam and trifloxysulfuron might slow the spread of rhizome johnsongrass enough to allow an increased number of ratoon crops before sugarcane fields need to be replanted.

Energycane has been proposed as a potential, perennial bioenergy crop for lignocellulosic-derived fuel production in the United States. Herbicides currently used in sugarcane and other crops can potentially be used in energycane if there is acceptable tolerance. Also, to limit future invasion of energycane escapes, herbicides used for perennial grass control could potentially be used for management of escapes. In container studies conducted outside, aboveground and belowground biomass of energycane was measured to evaluate energycane tolerance to 9 PRE and 19 POST herbicides used in sugarcane and other crops. PRE application of atrazine, diuron, mesotrione, metribuzin, pendimethalin, and S-metolachlor at rates labeled for sugarcane did not significantly injure (< 3%) or reduce energycane biomass compared with the nontreated plants 28 and 56 d after treatment (DAT). Injury from clomazone (54%), flumioxazin (7%), and hexazinone (29%) was observed 28 DAT. Injury from flumioxazin was transient and was not observed at 56 DAT. At 56 DAT, energycane injury increased to 71 and 98%, respectively, for clomazone and hexazinone. Hexazinone and clomazone applied PRE significantly reduced biomass compared with the nontreated plants. At 28 DAT, POST application of 2,4-D amine, ametryn, asulam, atrazine, carfentrazone, dicamba, halosulfuron, mesotrione, metribuzin, and trifloxysulfuron at labeled rates for sugarcane did not injure or significantly reduce energycane biomass compared with the nontreated plants. Injury was observed when clethodim (99%), clomazone (51%), diuron (51%), flumioxazin (21%), glufosinate (84%), glyphosate (100%), hexazinone (100%), paraquat (66%), and sethoxydim (100%) were applied POST, and each of these treatments reduced energycane biomass compared with the nontreated plants. These results show that several PRE and POST herbicides used for weed management in sugarcane may potentially be used in energycane for weed control. Also, based on our results, clethodim, glyphosate, and sethoxydim would be effective for management of energycane escapes.

Giant reed has been proposed as a bioenergy crop in the sugarcane production region of south Florida, where it has a high invasive potential. In an effort to limit future invasion of giant reed escapes in sugarcane, currently labeled sugarcane herbicides asulam and trifloxysulfuron were evaluated for its management. Greenhouse and field dose–response studies were conducted at the Everglades Research and Education Center in Belle Glade, FL, between 2010 and 2011. Herbicides were applied at rates ranging from 0.46 to 7.4 kg ha−1 asulam and 2 to 32 g ha−1 trifloxysulfuron, which represent 0.125× to 2× sugarcane labeled use rates, respectively. In the greenhouse, asulam and trifloxysulfuron reduced giant reed relative shoot dry weight by a maximum of 50% at 21 d after treatment (DAT). The probability of giant reed resprouting 35 d following herbicide treatment was greater for trifloxysulfuron when compared with asulam. In the field, it was predicted that a maximum of 69 and 55% giant reed control occurred with application of asulam and trifloxysulfuron, respectively, at 14 DAT. Relative shoot dry weight of giant reed treated with asulam and trifloxysulfuron was reduced by a maximum of 43% at 42 DAT. Application of asulam and trifloxysulfuron did not provide complete control of giant reed at twice the labeled sugarcane use rate, indicating that control of established giant reed in sugarcane with currently available herbicides would not be an option.

Tropical signalgrass is one of the most serious weed problems in the St. Augustinegrass sod production in Florida, and its presence increases production costs and lowers turfgrass quality. The objectives of our research were to: (1) evaluate herbicides preemergence and postemergence for control of tropical signalgrass and (2) compare control of tropical signalgrass and other problem weeds (torpedograss, blanket crabgrass, and India crabgrass) with postemergence herbicides. In preemergence herbicide field trials, only benefin + oryzalin, imazapic, imazapic + 2,4-D, and oryzalin provided ≥75% tropical signalgrass control 8 wk after application (WAA). By 11 WAA, only benefin + oryzalin and imazapic + 2,4-D provided ≥75% tropical signalgrass control. In greenhouse experiments, eight herbicide treatments were applied postemergence to tropical signalgrass seedlings at the two-, four-, six-, and eight-leaf stages. Asulam and CGA 362622 provided ≥89% tropical signalgrass control at all application timings. Imazaquin controlled tropical signalgrass ≥98% when applied before the eight-leaf stage. However, in field trials with mature tropical signalgrass (>20 cm stolons), none of the 20 herbicide treatments applied postemergence provided acceptable control.

Elephantgrass has been proposed as a potential feedstock for biofuel production in south Florida. To limit future invasion of escapes in sugarcane and vegetables, the response of newly established elephantgrass to glyphosate, clethodim, sethoxydim, asulam, and trifloxysulfuron was determined using dose–response curves. Log-logistic models were used to determine the herbicide dose required to produce 90% growth reduction (GR90). The GR90 values for shoot biomass at 21 d after treatment (DAT) were 477 g ae ha−1 of glyphosate, 262 g ai ha−1 of clethodim, 381 g ai ha−1 of sethoxydim, 12 kg ai ha−1 of asulam, and 94 g ai ha−1 of trifloxysulfuron. The GR90 values for root biomass at 35 DAT were 570 g ae ha−1 of glyphosate, 257 g ai ha−1 of clethodim, 432 g ai ha−1 of sethoxydim, 17 kg ai ha−1 of asulam, and 183 g ai ha−1 of trifloxysulfuron. Elephantgrass was predicted to exhibit 97, 98, 75, 1, and 5% mortality after application of glyphosate, clethodim, sethoxydim, asulam, and trifloxysulfuron, respectively, at the label use rates 35 DAT. Results suggest that glyphosate and clethodim will provide control of newly established elephantgrass at label use rates for spot treatments and in vegetables, respectively. Rates higher than the label use rate of sethoxydim will be required to provide acceptable control of newly established elephantgrass in vegetables. However, newly established elephantgrass was not controlled by asulam and trifloxysulfuron at label use rates, implying that control of escapes will be difficult in sugarcane.