Immediate posttreatment irrigation has been proposed as a method to reduce hybrid bermudagrass [Cynodon dactylon (L.) Pers. × Cynodon transvaalensis Burtt Davy] phytotoxicity from topramezone. Immediate irrigation is impractical, because it would take a turfgrass sprayer 10 to 15 min to cover an average golf course fairway or athletic field. There is also insufficient evidence regarding how posttreatment irrigation, immediate or otherwise, influences mature goosegrass [Eleusine indica (L.) Gaertn.] control from topramezone or low-dose topramezone plus metribuzin programs. We sought to investigate bermudagrass and E. indica response to immediate, 15-min, and 30-min posttreatment irrigation compared with no irrigation following topramezone at 12.3 g ae ha−1, the lowest labeled rate, or topramezone at 6.1 g ha−1 plus metribuzin at 210 g ai ha−1. We also evaluated placement of each herbicide and their combination on soil, foliage, and soil plus foliage to help elucidate the mechanisms involved in differential responses between species and herbicide mixtures. Responses were largely dependent on trial due to bermudagrass injury from high-dose topramezone being nearly eliminated by immediate irrigation in one trial and only slightly affected in another. When posttreatment irrigation was postponed for 15 or 30 min, topramezone alone injured bermudagrass unacceptably in both trials. Bermudagrass was injured less by low-dose topramezone plus metribuzin than by high-dose topramezone. All posttreatment irrigation timings reduced E. indica control compared with no posttreatment irrigation. The herbicide placement study suggested that topramezone control of E. indica is highly dependent on foliar uptake and that phytotoxicity of both bermudagrass and E. indica is greater from topramezone than metribuzin. Thus, posttreatment irrigation likely reduces topramezone rate load with a concomitant effect on plant phytotoxicity of both species. Metribuzin reduced 21-d cumulative clipping weight and tiller production of plants, and this may be a mechanism by which it reduces foliar white discoloration from topramezone.

Goosegrass [Eleusine indica (L.) Gaertn.] remains problematic for bermudagrass [Cynodon dactylon (L.) Pers.] turf managers due to the ineffective, selective control of mature plants with available postemergence herbicides and lack of sufficient residual activity from those herbicides to control seedling plants. Topramezone controls mature E. indica, but past efforts to suppress potential injury to bermudagrass turf have been inconsistent. We hypothesized that metribuzin at 210 g ai ha−1 in admixture with topramezone would improve bermudagrass tolerance while conserving mature E. indica control. In preliminary field studies, metribuzin mixed with topramezone at 1.2 or 2.5 g ae ha−1 applied twice at a 3-wk interval reduced bermudagrass injury and white discoloration compared with topramezone applied alone, but metribuzin did not safen bermudagrass to mesotrione. Topramezone at 3.7 g ha−1 plus 210 g ha−1 metribuzin applied twice at a 3-wk interval offered improved bermudagrass tolerance while it still controlled mature E. indica during 15 field and 2 greenhouse studies in Virginia. This program offered a 10-fold decrease in suprathreshold duration of white discoloration compared with topramezone alone at 6.1 g ha−1. Bermudagrass absorbed three times less radioactivity than E. indica at timings up to 48 h after treatment with [14C]topramezone. Bermudagrass also metabolized twice as much topramezone compared with E. indica at 48 h after treatment. Metribuzin reduced 14C absorption by approximately 25% in both species. These studies confirm the performance of a novel, low-dose topramezone plus metribuzin program for mature E. indica control in bermudagrass turf and suggest that selectivity between bermudagrass and E. indica to topramezone is due to differential absorption and metabolism. The fact that metribuzin reduces topramezone absorption in both species suggests that it may help reduce bermudagrass phytotoxic response to topramezone, but its role in altering selectivity between bermudagrass and E. indica may be due to other factors.

Only four herbicides are registered for smooth crabgrass or goosegrass control on creeping bentgrass golf putting greens. None of the four herbicides control weedy grasses for the entire season or control weeds postemergence when applied once at labeled rates. Three of the product labels prohibit repeated use or application during stressful summer conditions. We hypothesized frequently applying herbicides at low doses could provide season-long control of summer grasses while minimizing turf injury. Seven field experiments were conducted on creeping bentgrass putting greens to evaluate various herbicides applied monthly, biweekly, or weekly for postemergence and residual control of goosegrass and smooth crabgrass as well as creeping bentgrass putting green tolerance. Metamifop applied twice monthly at 200 g ai ha−1, topramezone applied eight times weekly at 1.5 g ae ha−1, and siduron applied weekly at 5.6 kg ai ha−1 or four times biweekly at 11 kg ha−1 did not injure creeping bentgrass greater than 10% and maintained creeping bentgrass quality and cover equivalent to nontreated turf. Weekly or biweekly programs of fenoxaprop or quinclorac caused unacceptable injury and quality decline. Metamifop applied monthly and either fenoxaprop program controlled both smooth crabgrass and goosegrass by 97% to 99% throughout the growing season. Programs containing either quinclorac or siduron controlled smooth crabgrass by 99% to 100% but did not control goosegrass greater than 39%. All topramezone programs controlled smooth crabgrass by 69% to 77% and goosegrass by 93% to 98%. In additional studies, siduron applied five times biweekly did not injure creeping bentgrass on putting greens and controlled smooth crabgrass by more than 90% at seasonal, cumulative rates between 17 and 65 kg ai ha−1. This method of frequent, low-dose herbicide treatment to control smooth crabgrass and goosegrass on golf putting greens is novel and currently could be legally implemented with siduron.

In this study, we characterized secretory granules in somatotroph (STCs) and corticotroph (CTCs) cells from the anterior pituitary of rats, in conjunction with different experimental treatments with bee venom (BV). In the rats injected for 30 days with daily BV doses equivalent to one sting, we found significant changes in secretory granules' diameter: reduced by 48.15% in STCs and increased by 5.09% in CTCs, and especially a shift to gray into their intensity profile: increased by 237.04% in STCs and by 212.38% in CTCs. In the rats injected with a single high BV dose, the granules' diameter was reduced in both STCs (by 7.14%) and CTCs (by 4.67%—significant) and their gray intensity profile increased by 200% in STCs and by 51.71% in CTCs (both are significant). The changes in the gray profile reflected a reduced content of granules in the cells, consistent with an increase of the plasma levels of GH and ACTH in all cases. We concluded that the reduced hormone cargo of granules in STCs and CTCs resulted from an accelerated cell secretion. The results obtained for the two types of cells correlated, indicating a similar reaction of these secretory cells to the prolonged and acute presence of BV in the organism.

Aryloxyphenoxypropionate (AOPP) herbicides are used to control bermudagrass contamination in various turfgrasses. Applying AOPP herbicides alone can cause unacceptable injury to zoysiagrass but injury can be reduced when tank-mixed with triclopyr. There are limited data illustrating the extent of bermudagrass control and zoysiagrass cultivar tolerance when these compounds are combined. Research was conducted to determine the efficacy of multiple AOPP herbicides applied alone and tank-mixed with triclopyr for bermudagrass control in zoysiagrass turf. Treatments include three sequential applications of cyhalofop (0.32 kg ai ha−1), fenoxaprop (0.14 kg ha−1), fluazifop (0.11 kg ha−1), or quizalofop (0.09 kg ha−1) applied alone and tank-mixed with triclopyr (1.12 kg ae ha−1) applied to ‘Tifway’ bermudagrass, and ‘Diamond’, ‘Palisades’, and ‘Zenith’ zoysiagrass. Tifway bermudagrass control ranged from 41 to 69% and digital image analysis turf coverage data ranged from 18 to 50% for AOPP herbicides applied alone. The addition of triclopyr to AOPP herbicides increased bermudagrass control (64–79%) and reduced turf coverage (8–29%). Palisades and Zenith zoysiagrass exhibited less injury (1–18%) and greater turf coverage (84–86%) when AOPP herbicides were tank-mixed with triclopyr compared to AOPP herbicides applied alone. Diamond zoysiagrass was not tolerant to any AOPP herbicides applied alone or tank-mixed with triclopyr, except for fluazifop alone (18% injury and 93% turf coverage). Visual ratings and digital image analysis turf coverage data had a strong negative correlation over all tested turfgrasses. In general, AOPP herbicides plus triclopyr will control bermudagrass greater and injure zoysiagrass less compared to AOPP herbicides applied alone; however, these mixtures can cause unacceptable injury to Diamond zoysiagrass.

Turfgrass weed scientists commonly use visual ratings (VR) to assign a numerical value to a turfgrass or weed response. These ratings lack quantifiable numerical values and are considered subjective. Alternatives to VR, including line intersect analysis (LIA) and digital image analysis (DIA), have been used to varying extents in turfgrass research. Alternatives can be expensive, labor intensive, and can require extensive calibration and increased time for data acquisition. Minimal research has been conducted evaluating rating methods used in turfgrass weed science. Trials were conducted in 2007 and 2008 to evaluate ratings methods used to quantify large crabgrass populations as influenced by tall fescue mowing height (2.5, 5.1, 7.6, and 10.2 cm). Percent large crabgrass cover was assessed utilizing VR, LIA, and DIA to determine if differences existed among evaluation methods. Pairwise comparisons, Pearson's correlation, and linear regression were performed to compare evaluations. All rating methods were significantly correlated to one another. Differences of large crabgrass cover estimates existed between LIA and DIA data at all mowing heights and between VR and DIA data at the 7.6 and 10.2 cm mowing heights in 2007. Authors believe that shadows produced by the turf canopy at higher (≥ 7.6 cm) mowing heights increased DIA estimates of large crabgrass cover. At trial initiation in 2007, researchers did not capture calibration images because the methodology to eliminate a shadow influence using a standard digital image had not been published. Additional DIA calibration in 2008 corrected for canopy shadows, and no differences were observed in large crabgrass cover between all evaluation methods indicated by nonsignificance pairwise comparisons and estimated regression parameters. These data indicate VR are no different than LIA or DIA in estimating large crabgrass cover as affected by tall fescue mowing height.

Growth chamber experiments were conducted to assess the effects of foliage-only, soil-only, and foliage-plus-soil placements of amicarbazone on annual bluegrass and creeping bentgrass growth. Evaluated herbicide treatments included amicarbazone at 49 or 147 g ai ha−1, as well as bispyribac-sodium at 74 g ai ha−1 for comparative purposes. Data from this research agree with previous reports of amicarbazone plant uptake. Amicarbazone is absorbed via above- and belowground pathways; however, plant growth is inhibited more by root uptake. Compared to foliage-only amicarbazone placement, soil-only placement more than doubled reductions in aboveground biomass and root mass 56 d after treatment (DAT), whereas no differences were detected between placements including soil contact. Across all evaluated parameters in this research, amicarbazone (49 g ha−1) impacted creeping bentgrass growth similarly to bispyribac-sodium, whereas annual bluegrass growth was inhibited more by amicarbazone, suggesting it provides a more efficacious chemical option for end-user applications.

Evaluation of turfgrass performance at low nitrogen fertility levels is important because many home lawns are fertilized below common recommendations. The objective of this study was to evaluate visible quality and weed susceptibility of common and alternative cool season grasses under multiple management regimes in Wisconsin. A split-split plot completely randomized block design was used to evaluate ‘Kingfisher' Kentucky bluegrass (Kentucky bluegrass), ‘Kenblue' Kentucky bluegrass, ‘Victory II' chewings fescue, ‘Grande II' tall fescue, and ‘Jiffe II' perennial ryegrass. Each species was mowed at 3.5, 6.0, or 8.5 cm, and fertilized with 0, 98, or 196 kg ha−1 yr−1 of nitrogen. Visible quality and weed cover were evaluated four times annually for 3 yr. Tall fescue had the greatest turf quality across all treatments. Kingfisher Kentucky bluegrass, an improved variety, responded most dramatically to nitrogen fertilization, with quality rating improved from 5.1 to 7.1 when annual nitrogen applications totaled 196 kg ha−1 compared to the nonfertilized control. Kenblue Kentucky bluegrass, a common variety, had the greatest weed cover at all mowing heights and fertilizer rates. Assessment of common dandelion flowers by digital image analysis revealed that improved and common Kentucky bluegrass had greater common dandelion cover than fine or tall fescue when herbicides were withheld for 2.5 yr. Background soil fertility was found to have a significant impact on visible quality and weed cover. In an area with eroded, low-fertility soil, improved Kentucky bluegrass required 196 kg N ha−1 yr−1 to maintain high quality and limit weed invasion. These results suggest that tall fescue is best suited to low and high input conditions, while improved varieties of Kentucky bluegrass performed acceptably only under high inputs.

Amicarbazone is a photosystem II–inhibiting herbicide recently registered for annual bluegrass control in established turf systems that include creeping bentgrass. However, research to date reveals potential issues with creeping bentgrass tolerance to amicarbazone. Currently, the plant-growth regulator paclobutrazol is widely adopted by turf managers for chemical annual bluegrass suppression in creeping bentgrass putting greens. Field experiments were conducted throughout North Carolina in the spring of 2010 and 2011 to assess treatment regimens that included amicarbazone (49, 65, or 92 g ai ha−1) and paclobutrazol (70, 140, or 280 g ai ha−1) applied alone, as tank-mixtures, or used in tandem, at varying rates and sequential timings for annual bluegrass control in creeping bentgrass putting greens. In general, regimens including both compounds provided greater annual bluegrass control and acceptable turfgrass tolerance compared with stand-alone applications of amicarbazone at 8 and 12 wk after initial treatment (WAIT). When comparing regimens that included amicarbazone at 49 or 65 g ha−1, creeping bentgrass tolerance was greater for the higher application rate applied less frequently. These results indicate amicarbazone usage on creeping bentgrass greens may be beneficially affected with the incorporation of paclobutrazol to treatment regimens because annual bluegrass control with the combination was equal to or greater than stand-alone amicarbazone applications, and creeping bentgrass tolerance was superior 8 and 12 WAIT.