Working equids support the livelihoods of millions of low-income households worldwide and face several welfare challenges. Although equipment-related wounds are common, little is known about specific risk factors. This cross-sectional study surveyed equids used for cart-work in three Ethiopian towns. Number, size, severity and location of wounds were recorded for each animal, as well as work equipment characteristics and indicators of equipment fit and assembly. Questionnaires were conducted with each cart-driver focusing on equipment practices and attitudes. Logistic regression models were generated to investigate associations between equipment-related wounds (outcome) and equipment, work, driver and animal-related factors (predictors). In total, 369 equids and cart-drivers were surveyed. The prevalence of equipment-related wounds was 72.6% (268/369) with girth wounds being the predominant wound type in horses (50%; 122/244) while donkeys had predominantly shaft-related wounds (59%; 72/122). Donkeys were two times more likely to have equipment-related wounds than horses. The presence of equipment-related wounds was associated with factors such as previously having wounds, inadequate collar positioning and using purchased equipment compared to partly home-made equipment. Characteristics of specific equipment components were also associated with certain wound types, such as narrow saddle pressure points and saddle wounds. Equipment-related wounds are highly prevalent in working equids, representing a serious welfare concern. Factors relating to equipment design, fit and assembly were associated with the presence of wounds. Importantly, species differences require tailoring of preventive approaches amongst working equids. A better understanding of work equipment should therefore be promoted as part of wound prevention and animal welfare strategies.

Cystic echinococcosis (CE) is endemic in humans and domestic animals in eastern Africa. All the species of the Echinococcus granulosus sensu lato complex have been reported in this region except for E. equinus, possibly due to the small number of studies involving equids. This study reports the frequency of different Echinococcus species in donkeys from eastern Africa. A total of 5961 donkeys were examined during meat inspection in 3 slaughterhouses in Kenya. Identification of Echinococcus spp. was achieved through polymerase chain reaction-restriction fragment-length polymorphism and sequencing of the mitochondrial nicotinamide adenine dinucleotide (NADH) dehydrogenase subunit 1 gene. The prevalence of CE was 5.7% (337/5961). The 263 genotyped cysts belonged to E. equinus (n = 163), E. granulosus sensu stricto (n = 70), E. canadensis (G6/7) (n = 26) and E. ortleppi (n = 4). One donkey harboured a metacestode of Spirometra theileri. All E. equinus cases, except 2, originated from southern Ethiopia, whereas the other species were more evenly distributed across the study area. Most of the cysts belonging to E. equinus were fertile (111/163), while those of the other species were non-fertile. This is the first report of Echinococcus spp. in donkeys from sub-Saharan Africa and the first confirmation of E. equinus in East Africa. The frequent fertility of E. equinus cysts in donkeys affirms their suitability as intermediate hosts of this species, while low frequency and cyst fertility suggest a marginal role of donkeys in the transmission of E. granulosus s. s., E. canadensis (G6/7) and E. ortleppi.

Welfare issues relevant to equids working in developing countries may differ greatly to those of sport and companion equids in developed countries. In this study, we test the observer reliability of a working equine welfare assessment, demonstrating how prevalence of certain observations reduces reliability ratings. The assessment included behaviour, general health, wounds, and limb and foot pathologies. In Study 1, agreement between five observers and their trainer (the ‘gold standard’) was assessed using 80 horses and 80 donkeys in India. Intra-observer agreement was later tested on 40 of each species. Study 2 took place in Egypt, using nine observers, their trainer, 30 horses and 30 donkeys, adjusting some scoring systems and providing observers with more detailed guidelines than in Study 1. Percentage agreements, Fleiss kappa (with a weighted version for ordinal scores) and prevalence indices were calculated for each variable. Reliability was similar across both studies, but was significantly poorer for donkeys than horses. Age, sex, certain wounds and (for horses alone) body condition, consistently attained clinically-useful reliability. Hoofhorn quality, point-of-hock lesions, mucous membrane abnormalities, limb-tether lesions, and skin tenting showed poor reliability. Reporting the prevalence index alongside the percentage agreement showed that, for many variables, the populations were too homogenous for conclusive reliability ratings. Suggestions are made for improving scoring systems showing poor reliability, but future testing will require deliberate selection of a more diverse equine population. This could prove challenging given that, in both populations of horses and donkeys studied here, many pathologies apparently showed 90-100% prevalence.

Equine piroplasmosis (EP) is a tick-borne disease of economic importance, relevant in the international movement of equids. The causative agents are at least two apicomplexan protozoan parasites Babesia caballi and Theileria equi. To date, there is no study that estimates global and regional exposure of equids to EP. We therefore conducted a systematic review and meta-analysis to estimate the pooled prevalence and heterogeneity of EP using random-effects model. Six electronic databases were searched for publications on EP and assessed according to Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guidelines. A total of 66 eligible studies published between 1990 and 2019 and representing 24 041 equids were included. The overall pooled prevalence estimates (PPEs) of B. caballi was 22.3% (95% CI 21.7–22.8), while the overall PPE for T. equi was 29.4% (95% CI 28.7–30.0). The overall pooled prevalence due to co-infection with both parasites was 11.8% (95% CI 11.32–12.32). Also, subgroup analysis according to sex, age, diagnostic technique, equid species, region and publication years showed a substantial degree of heterogeneity across studies computed for both B. caballi and T. equi infections in equids. Awareness of the current status of EP globally will alert the relevant authorities and stakeholders where necessary on the need for better preventive and control strategies against the disease.

Donkeys facilitated trade and transport in much of the ancient world, but were seldom used in elite or leisure activities. While Tang Dynasty (AD 618–907) texts indicate that noble women played polo riding donkeys, this has never been documented archaeologically. Here, the authors present the first archaeological evidence of the significance of donkeys for elite Tang women through analyses of donkey remains recovered from the tomb of a Tang noblewoman in Xi'an, China. These findings broaden our understanding of the donkey's historic roles beyond simple load bearing.

The celebrated conservationist, and serial memoirist, Gerald Durrell often imaginatively revisited the Corfu of his childhood. Donkeys were integral to his vision of Greek rural life. Both the setting and the style of his literary output resisted what he regarded as unwelcome modernization. His 1968 publication The Donkey Rustlers, one of his few novels, shows how Durrell attempted to perpetuate an outdated view of both Greece and children's literature. It is argued that Durrell's well-attested affection for the Greek people was not well reflected by a narrative in which both foreign children and donkeys seem to come out on top.

Toxoplasmosis is one of the five parasitic diseases considered as a priority for public health action. The consumption of raw milk products represents a possible risk, in particular for certain categories of people. The aim of this study was to evaluate the possible effects of Toxoplasma gondii on milk yield and quality in sero-positive animals with parasitemia. Eighteen healthy lactating Amiata jennies, between 90 and 180 days were included in the study. Four donkeys scored positive for immunofluorescent antibody test (IFAT), and each IFAT positive donkey presented parasitic DNA both in the blood and milk. No significant differences were found between milk yield in PCR-positive donkeys compared with the negative cases, however the former tended to have a greater production. Milk quality in the positive donkeys showed a significantly lower percentage of casein (0.72% v. 0.81%) and ash (0.32% v. 0.37%). Positive cases had a highly significant larger average diameter of globules (2.35 µm) and fewer globules/ml (2.39×108). Somatic cell and bacterial counts were normal and in agreement with the literature. Toxoplasma gondii did not seem to present clinical forms in lactating jennies. Further in vivo studies are needed to further assess the risk of T. gondii transmission through donkey milk, together with the impact of different stages of infection on milk quality.

The work performance of two teams of four donkeys (heavy, 680 kg and light, 460 kg) and one pair of Jersey crossbred oxen (646 kg) was compared when they ploughed 4 hi day on four types of soil (clay, redsoil, sandy soil and sandy clay) using two types of plough, a conventional ox plough (40 kg) and a lighter prototype, the ‘Walco’ plough (32 kg) on an experimental farm. Work parameters were also measured with farmers’ cattle and donkey teams ploughing on f arms in Matobo and Nkayi districts. Working speed, power and effective field capacity (ETC) were higher for the ox-team (1·03 m/s, 920 W and 14·5 h/ha for the conventional plough and 0·99 m/s, 745 W and 13·9 h/hafor the Walco plough) and the heavier donkey team (0·87 m/s, 689 W and 14·2 h/hafor the conventional plough and 0·87 m/s, 787 W and 17·3 h/hafor the Walco plough) than for the lighter donkey team (0·59 m/s, 461 W and 22·1 h/hafor the conventional plough and 0·64 m/s, 445 W and 23·4 h/hafor the Walco plough). Expressed as a proportion of live weight or metabolic live weight there were no significant differences in draught forces exerted between teams but power output per unit live weight was greater in the ox-team than in the light donkey team but similar to that in the heavy donkey team. The Walco plough required a lower force (742 N) to operate than the conventional plough (816 N) but apart from this did not have any marked advantages over the conventional plough. On-farm, team sizes of donkeys varied from three to seven animals (team weight 340 kg to 1007 kg) and cattle team sizes from two to four animals (team weights 558 to 1709 kg). Regardless of team number, the heavier teams tended to out-perform the lighter teams (speed range 0·63 to 1·08 m/s, power 395 to 1136 W, EFC 9·1 to 25 h/ha)) with one exception, a well trained team of two oxen (team weight 879 kg, speed 1·02 m/s, power 775 W, EFC 9·1 h/ha). Donkeys tended to plough at a slower pace than oxen, with a lower power output, although when weight differences between teams were equalized (four heavy donkeys compared with two oxen), then there was little to chose between the species. Results suggested that teams of three or more donkeys can effectively be used for ploughing on the soils tested. The results highlighted the importance that team live weight and training/experience have in determining work performance.

Three experiments were undertaken to assess the Zimbabwean donkey. In the first study, 191 male and 144 female working donkeys from Matopos, Nkayi and Matobo districts were weighed. Age, sex and coat colour were recorded and heart girth, umbilical girth, body length and height were measured. There were no differences (P > 0⋅05) in live weight, 142 and 141 kg, heart girth 115 and 115 cm, body length 89 and 90 cm and height 105 and 105 cm between males and females. This suggests that draught potential might be similar between the sexes. Heart girth was the best single predictor of live weight: live weight (kg) = heart girth (cm)2⋅83/4786 (R2 = 0⋅86). Donkeys were similar in size to others in Africa. In the second study, the voluntary dry-matter intake (DMI) of a poor quality hay was measured for 35 days in nine male (mean live weight 150 kg) and nine female donkeys (142 kg) allocated to one of three treatment groups: water available ad libitum, or given every 48 h, or every 72 h. There were significant differences in daily water (P < 0.001) and DMIs (P < 0.05): 8.5, 4.9 and 5.1l and 3.1, 2.8 and 2.7 kg for the three treatment groups, respectively. However even with restricted access to water, donkeys maintained a relatively high DMI. In the third experiment a 3 Х 3 Latin square was designed with three teams of four male donkeys each, either working (5 h/day) I no access to food (5 h/day); not working/no access to food (5 h/day) or not working/access to food 24 h/day, for 63 days. For working and non-working donkeys, there were no significant differences (P > 0.05) in DMI, DM apparent digestibility and mean retention time (MRT) of hay. Time of access to food did not influence DMI. The apparent lack of response was attributed primarily to the poor quality of the hay.

Measurements have been made of the length and diameter of the cervix and caudal ostium position in relation to the vaginal floor in 30 jenny asses of Baudet du Poitou breed and crosses. These measurements were obtained on the 4th, 5th and 6th days of oestrus and the 6th and 8th days of dioestrus. The observed anatomical modifications were mainly associated with the animal's physiological state, age and morphological type (size). The cervical anatomy of the female donkey differs from that of the mare not only in size but also in the existence of a large cervical protrusion into the vagina which can lead to the occurrence of cervical lesions during parturition or from the mechanical action of mating, especially when the female is small. This anatomical conformation also makes intrauterine ejaculation difficult whether by natural service or by artificial insemination.

The extra energy used for walking on the level and on negative gradients above that used when standing still (Ew) (J/m per kg live weight) was measured in two entire male donkeys (Equus asinus). Ew was not affected by speed within the measured range (V = 0·6 to 1·3 m/s) but gradient (0, −10%, −15%) had a significant effect Ew−10% = 0·97 (s.e. 0·02), Ew−10% = 0·55 (s.e. = 0·03) and Ew−15% = 0·67 (s.e. 0·03).

The extra energy cost of carrying loads (Ec), defined as J/m per kg carried was measured using the same animals. Loads were placed over the animals shoulders and speed was varied within the range 0·6 to 1·3 m/s (Eclevel = 1·1 (s.e. 0·04), Ec−10% = 2·7 (s.e. 0·17) and Ec−15% = 3·3 (s.e. 0·20) were significantly different.

The energy cost of pulling loads (Ep) (f/m per kg) was measured while the animals pulled loads up to proportionately 0·17 of their live weight. The animals wore a breast-plate harness and walking speed was varied within the range 0·6 to 1·3 m/s. Mean values were 26·5 (s.e. 0·72) on the level, 15-3 (s.e. 1·2) on the −10% gradient and 6·2 (s.e. 0·43) on the −15% gradient.

The two donkeys used in this experiment were more efficient in both carrying and pulling loads than oxen and buffaloes. Negative gradients have a significant effect on energy consumption and when estimating the energy expenditure of working animals this factor should be taken into account.