Recent research reported synergism between glufosinate plus very low rates of protoporphyrinogen oxidase (PPO)–inhibiting herbicides on select broadleaf weeds. Two field studies, each consisting of four trials, were conducted in 2020 and 2021 in commercial fields with glyphosate-resistant (GR) horseweed in Ontario, Canada. Study 1 evaluated GR horseweed control with glufosinate plus five PPO inhibitors at 5% of the label rate; study 2 evaluated what dose of saflufenacil is needed when co-applied with glufosinate to improve GR horseweed control. In study 1, glufosinate plus very low rates of PPO-inhibiting herbicides provided low GR horseweed control. At site 1, despite the synergistic increase in GR horseweed control with saflufenacil (1.25 g ai ha–1) plus glufosinate (300 g ai ha–1), the level of control did not exceed 42% at 2 and 4 wk after application (WAA); the interaction was additive at 8 WAA. The co-application of glufosinate (300 g ai ha–1) with pyraflufen-ethyl (0.34 g ai ha–1), pyraflufen-ethyl/2,4-D (26.4 g ai ha–1), flumioxazin (5.35 g ai ha–1), fomesafen (12 g ai ha–1), or sulfentrazone (7 g ai ha–1) resulted in an additive interaction for GR horseweed control at 2, 4, and 8 WAA. However, glufosinate plus pyraflufen-ethyl or sulfentrazone was antagonistic at 8 WAA. In study 2, similar doses of saflufenacil were required for 50%, 80%, and 95% GR horseweed control whether glufosinate was included in the mixture or not. Interactions between glufosinate (300 g ai ha–1) plus saflufenacil at 1.56, 3.13, 6.25, and 12.5 g ai ha–1 were antagonistic at 2, 4, and 8 WAA at sites 1, 2, and 3; all other interactions were additive. The results of this research indicate there was little to no benefit of adding very low rates of PPO-inhibiting herbicides to glufosinate to improve GR horseweed control under field conditions.

Glyphosate resistance in weed species has presented immense challenges for farmers in Ontario. The co-application of burndown plus residual herbicides provides control of glyphosate-resistant (GR) horseweed in soybean. Pyraflufen-ethyl/2,4-D is a premixed herbicide formulation sold under the tradename Blackhawk®. Five field experiments were conducted over a 2-yr period (2019, 2020) in fields in southwestern Ontario to ascertain the biologically effective dose of pyraflufen-ethyl/2,4-D, applied alone, or mixed with metribuzin, for GR horseweed control when applied preplant to soybean. Soybean visible injury for all treatments was <15%. At 8 wk after application (WAA), the calculated doses of pyraflufen-ethyl/2,4-D for 50%, 80%, and 95% control of GR horseweed were 390, 1,148, and >2,108 g ha−1, respectively. The addition of metribuzin to pyraflufen-ethyl/2,4-D reduced the doses of pyraflufen-ethyl/2,4-D for 50%, 80%, and 95% control of GR horseweed to 19, 46, and 201 g ha−1, respectively. Pyraflufen-ethyl/2,4-D + metribuzin controlled GR horseweed by 97%, which is comparable to the current industry standards. Based on these results, pyraflufen-ethyl/2,4-D + metribuzin (527 + 400 g ha−1) applied preplant can be used for GR horseweed control in soybean.

Wild poinsettia (Euphorbia heterophylla L.) is a troublesome broadleaf weed in grain production areas in South America. Herbicide resistance to multiple sites of action has been documented in this species, including protoporphyrinogen oxidase (PPO) inhibitors. We investigated the physiological and molecular bases for PPO-inhibitor resistance in a E. heterophylla population (RPPO) from Southern Brazil. Whole-plant dose–response experiments revealed a cross-resistance profile to three different chemical groups of PPO inhibitors. Based on dose–response parameters, RPPO was resistant to lactofen (47.7-fold), saflufenacil (8.6-fold), and pyraflufen-ethyl (3.5-fold). Twenty-four hours after lactofen treatment (120 g ha−1) POST, RPPO accumulated 27 times less protoporphyrin than the susceptible population (SPPO). In addition, RPPO generated 5 and 4.5 times less hydrogen peroxide and superoxide than SPPO, respectively. The chloroplast PPO (PPO1) sequences were identical between the two populations, whereas 35 single-nucleotide polymorphisms were found for the mitochondrial PPO (PPO2). Based on protein homology modeling, the Arg-128-Leu (homologous to Arg-98-Leu in common ragweed [Ambrosia artemisiifolia L.] was the only one located near the catalytic site, also in a conserved region of PPO2. The cytochrome P450 monooxygenase inhibitor malathion did not reverse resistance to lactofen in RPPO, and both populations showed similar levels of PPO1 and PPO2 expression, suggesting that metabolic resistance and PPO overexpression are unlikely. This is the first report of an Arg-128-Leu mutation in PPO2 conferring cross-resistance to PPO inhibitors in E. heterophylla.

The widespread occurrence of Palmer amaranth resistant to acetolactate synthase inhibitors and/or glyphosate led to the increased use of protoporphyrinogen oxidase (PPO)-inhibiting herbicides. This research aimed to: (1) evaluate the efficacy of foliar-applied fomesafen to Palmer amaranth, (2) evaluate cross-resistance to foliar PPO inhibitors and efficacy of foliar herbicides with different mechanisms of action, (3) survey the occurrence of the PPO Gly-210 deletion mutation among PPO inhibitor–resistant Palmer amaranth, (4) identify other PPO target-site mutations in resistant individuals, and (5) determine the resistance level in resistant accessions with or without the PPO Gly-210 deletion. Seedlings were sprayed with fomesafen (263 gaiha−1), dicamba (280 gaiha−1), glyphosate (870 gaiha−1), glufosinate (549 g ai ha−1), and trifloxysulfuron (7.84 gaiha−1). Selected fomesafen-resistant accessions were sprayed with other foliar-applied PPO herbicides. Mortality and injury were evaluated 21 d after treatment (DAT). The PPX2L gene of resistant and susceptible plants from a selected accession was sequenced. The majority (70%) of samples from putative PPO-resistant populations in 2015 were confirmed resistant to foliar-applied fomesafen. The efficacy of other foliar PPO herbicides on fomesafen-resistant accessions was saflufenacil>acifluorfen=flumioxazin>carfentrazone=lactofen>pyraflufen-ethyl>fomesafen>fluthiacet-methyl. With small seedlings, cross-resistance occurred with all foliar-applied PPO herbicides except saflufenacil (i.e., 25% with acifluorfen, 42% with flumioxazin). Thirty-two percent of PPO-resistant accessions were multiple resistant to glyphosate and trifloxysulfuron. Resistance to PPO herbicides in Palmer amaranth occurred in at least 13 counties in Arkansas. Of 316 fomesafen survivors tested, 55% carried the PPO Gly-210 deletion reported previously in common waterhemp. The PPO gene (PPX2L) in one accession (15CRI-B), which did not encode the Gly-210 deletion, encoded an Arg-128-Gly substitution. The 50% growth reduction values for fomesafen in accessions with Gly-210 deletion were 8- to 15-fold higher than that of a susceptible population, and 3- to 10-fold higher in accessions without the Gly-210 deletion.

Field trials were conducted to evaluate weed control and soybean tolerance with co-application of pyraflufen-ethyl and glyphosate. Pyraflufen-ethyl applied at 11, 23, or 45 g ai/ha in combination with glyphosate did not affect control of barnyardgrass, browntop millet, redroot pigweed, sicklepod, or smellmelon compared to glyphosate alone or co-applied with chlorimuron. Initial benefit (7 DAT) to pitted morningglory control with pyraflufen-ethyl plus glyphosate compared to glyphosate alone or co-applied with chlorimuron was not observed 28 DAT. Hemp sesbania control was increased by pyraflufen-ethyl at 45 g/ha plus glyphosate and glyphosate plus chlorimuron relative to glyphosate applied alone. Pyraflufen-ethyl plus glyphosate resulted in significant visual soybean injury, and a reduction in soybean yield was observed with pyraflufen-ethyl at 45 g/ha relative to glyphosate applied alone or in combination with chlorimuron. Results from a weed-free study confirmed crop injury and yield reduction potential with pyraflufen-ethyl plus glyphosate. Overall results suggest no benefit with respect to weed control or crop tolerance to pyraflufen-ethyl inclusion in a glyphosate-resistant soybean weed control program over that observed with currently registered co-application of glyphosate and chlorimuron.

Silvery threadmoss naturally reproduces through spore and bulbil production, both of which have potential to be controlled prior to establishment. Studies have not evaluated effects of turf protection products on moss protonema or gametophyte growth from spores or bulbils; consequently, most moss is controlled POST on putting greens. Initial studies were performed to determine the optimal growth temperature for spores and bulbils in sterile culture. Protonemata from spores grew optimally at 29.5 C and gametophytes from bulbils grew optimally at 22.5 C. Three subsequent in vitro studies were conducted to evaluate effects of turf protection products on moss development from spores or bulbils in axenic culture at a constant 24 C. Carfentrazone, which effectively controls mature silvery threadmoss gametophytes POST, also reduced green cover of moss protonemata and gametophyte production from spores and bulbils. All combinations with carfentrazone reduced area under the progress curve (AUPC) for green cover of moss for both spores and bulbils by 80% or more by 3 wk after treatment. Sulfentrazone, oxyfluorfen, oxadiazon, saflufenacil, flumioxazin, and pyraflufen-ethyl reduced AUPC of moss equivalent to carfentrazone for both propagule types. The two fosetyl-Al products, phosphite, and mineral oil caused an increase in silvery threadmoss cover between 22 and 113% of the nontreated for spores; however, only methiozolin positively influenced AUPC (90.2%) compared to the nontreated for bulbils. Though silvery threadmoss is typically targeted POST on putting greens, there are products that can provide PRE control, including the industry standard of carfentrazone. These data suggest that differences may occur between turf protection products in their ability to suppress silvery threadmoss establishment from spores or bulbils.

Desiccants are currently used to improve lentil dry-down prior to harvest. Applying desiccants at growth stages prior to maturity may result in reduced crop yield and quality, and leave unacceptable herbicide residues in seeds. There is little information on whether various herbicides applied alone or as a tank-mix with glyphosate have an effect on glyphosate residues in harvested seed. Field trials were conducted at Saskatoon and Scott, Saskatchewan, Canada, from 2012 to 2014 to determine whether additional desiccants applied alone or tank mixed with glyphosate improve crop desiccation and reduce the potential for unacceptable glyphosate residue in seed. Glufosinate and diquat tank mixed with glyphosate were the most consistent desiccants, providing optimal crop dry-down and a general reduction in glyphosate seed residues without adverse effects on seed yield and weight. Saflufenacil provided good crop desiccation without yield loss, but failed to reduce glyphosate seed residues consistently. Pyraflufen-ethyl and flumioxazin applied alone or tank mixed with glyphosate were found to be inferior options for growers as they exhibited slow and incomplete crop desiccation, and did not decrease glyphosate seed residues. Based on results from this study, growers should apply glufosinate or diquat with preharvest glyphosate to maximize crop and weed desiccation, and minimize glyphosate seed residues.