Mesotrione typically requires multiple applications to control emerged weeds in turfgrass. As it is absorbed by both foliage and roots, a controlled-release (CR) formulation could eliminate the need for multiple applications. Research was conducted to evaluate simulated-release scenarios that mimic a potential CR mesotrione formulation. A soluble-concentrate formulation of mesotrione was titrated to produce a stepwise change in mesotrione rates, which were applied daily to mimic predetermined release scenarios over a 3-wk period. CR scenarios were compared to a broadcast treatment of mesotrione at 280 g ai ha–1 applied twice at 3-wk intervals, and a nontreated. Mesotrione applied in three temporal-release scenarios controlled creeping bentgrass, goosegrass, nimblewill, smooth crabgrass, and white clover equivalent to the standard sprayed mesotrione treatment in every comparison. However, each CR scenario injured tall fescue two to seven times more than the standard treatment. Soil- and foliar-initiated repeat treatments were equivalent in most comparisons. Our data indicate that mesotrione applied in a temporal range to simulate controlled-release scenarios can deliver desired weed control efficacy comparable to sequential broadcast applications. More research is needed to elucidate proper timings and release scenarios to minimize turfgrass injury.

Weeds are often spatially aggregated in maize fields, and the level of aggregation varies across and within fields. Several annual weed species are present in maize fields before postemergence herbicide application, and herbicides applied will control several species at a time. The goal of this study was to assess the spatial distribution of multispecies weed infestation in maize fields. Ground-based imagery was used to map weed infestations in rain-fed maize fields. Image segmentation was used to extract weed cover information from geocoded images, and an expert-based threshold of 0.102% weed cover was used to generate maps of weed presence/absence. From 19 site-years, 13 (68%) demonstrated a random spatial distribution, whereas six site-years demonstrated an aggregated spatial pattern of either monocotyledons, dicotyledons, or both groups. The results of this study indicated that monocotyledonous and dicotyledonous weed groups were not spatially segregated, but discriminating these weed groups slightly increased the chances of detecting an aggregated pattern. It was concluded that weeds were not always spatially aggregated in maize fields. These findings emphasize the need for techniques allowing the assessment of weed aggregation prior to conducting site-specific weed management.

Oriental mustard seed meal (MSM), a byproduct generated by pressing the seed for oil, exhibits herbicidal properties. In turfgrass, soil fumigants such as methyl bromide are used to control weeds prior to renovation of turf. Environmental concerns have resulted in deregistration of methyl bromide, prompting the need for alternatives. The objective of this research was to determine the effect of MSM on the establishment of selected turfgrass weeds as well as inhibitory effects on establishment of desirable turfgrasses. Greenhouse experiments were conducted in 2006 and 2007 at the University of Missouri. MSM was amended in soil at 0, 1,350 (low), 2,350 (medium), and 3,360 kg ha−1 (high) concentrations. Weed species included annual bluegrass, large crabgrass, buckhorn plantain, white clover, and common chickweed. Turfgrass species included: Rembrandt tall fescue, Evening Shade perennial rye, and Riviera bermudagrass. All species were seeded into soil amended with MSM and either tarped or left untarped. All treatments were compared to dazomet (392 kg ha−1), a synthetic standard. Plant counts and biomass of all species were recorded 4 wk after seeding. Overall, tarped treatments suppressed weed emergence 27 to 50% more compared to untarped treatments, except for large crabgrass. High rates of MSM suppressed emergence of all weeds ≥ 63%. Compared to the untreated control, the density of buckhorn plantain, white clover, and common chickweed was reduced by ≥ 42% at low rates of MSM. Biomass of buckhorn plantain, annual bluegrass, common chickweed, white clover, and large crabgrass was reduced from 37 to 99% at high rates of MSM. MSM at high rates reduced stand counts of tall fescue and perennial ryegrass up to 81% and 77% respectively, compared to the untreated control. Regardless of MSM rates or tarping, suppression of common bermudagrass emergence did not exceed 30%; tarped treatments actually increased bermudagrass emergence by 22%. The biomass for tall fescue, perennial ryegrass, and bermudagrass was reduced by 85, 68, and 10%, respectively, at high rates of MSM. For tall fescue, MSM at all rates strongly suppressed seed germination by 7 d after planting (DAP) (up to 100%), with additional germination observed through 14 DAP, but not thereafter. In both trials, dazomet completely suppressed emergence of all weeds. MSM appears to suppress emergence and growth of a number of weeds common in turf, with potential selectivity for bermudagrass.

A 3-yr field study was conducted to determine the effect of a biological control agent, Sclerotinia minor Jagger, and a common herbicide, Killex, on the population dynamics of dandelion and other broadleaf species and on the dandelion seed bank. Treatments were applied as one spring, one early autumn, or a spring plus an early autumn treatment per year. The response of the dandelion population to a spring herbicide treatment was similar to two applications per year (spring and early autumn). Significantly less dandelion control occurred after the first early autumn application of the herbicide. Two weeks after application, spring or early autumn treatments with S. minor were equally effective in suppressing dandelions. In the second year of the two applications per year of S. minor treatment, weed control was equivalent to the herbicide. By the third year of the one spring application of S. minor, weed control was equivalent to the herbicide. Generally over the study period, the early autumn application of S. minor was less effective than the spring or the spring and early autumn applications. The S. minor treatments significantly reduced the dandelion seed bank, and this effect was not significantly different from the Killex herbicide treatment. The rate, frequency, and seasonal timing of application had no effect on the dandelion seed-bank size, but terminating the application would gradually replenish the seed bank. Populations of white clover, broadleaf plantain, birdsfoot trefoil, and common ragweed were similarly suppressed by either the S. minor or the herbicide treatments. Yellow woodsorrel significantly increased after 1 yr of herbicide treatment compared with the S. minor and untreated control treatments, indicating a possible weed species shift. Turf quality was improved because of the herbicide and S. minor treatments, but grass injury and smooth crabgrass invasion were recorded in 17% of herbicide-treated plots.

Clover inclusion may increase the sustainability of certain low-maintenance turfgrasses. However, selective weed control within mixed turfgrass–clover swards proves problematic because of clover susceptibility to herbicides. Research was conducted to identify common turf herbicides that are tolerated by three Trifolium species, including white clover, ball clover, and small hop clover, within low-maintenance turfgrass. Leaf and flower density, as well as plant height, were measured 4 wk after treatment as indicators of clover response to 14 herbicides. The three Trifolium spp. were moderately tolerant of bentazon (< 35% decrease in leaf density, height, or flowering). Simazine was well tolerated by white clover (< 5% decrease in all response variables), yet moderate injury to ball clover and small hop clover was observed (> 32% decrease in leaf density and > 27% decrease in flower density). Pronamide was well tolerated by white and ball clovers, with no effect on measured response variables; however, pronamide decreased small hop clover height and flower density (38 and 42%, respectively). Imazethapyr and imazamox were moderately well tolerated by white clover and small hop clover (< 39% decrease by all response variables), yet ball clover may be more susceptible to these herbicides than was anticipated based on previously reported tolerance. The herbicides 2,4-DB, halosulfuron, and metribuzin were well tolerated by white clover, with no effect on measured response variables; however, results suggest ball and small hop clovers were less tolerant. Clopyralid, 2,4-D, glyphosate, imazaquin, metsulfuron-methyl, and nicosulfuron resulted in varying degrees of injury across clover species and response variables, but, in general, these herbicides may not be viable options when attempting to maintain any of the three clover species tested. Further research is needed to quantify long-term effects of herbicide application on sward composition and clover succession.

Small broomrape, a holoparasitic weed, was recently introduced to the Pacific Northwest and contaminates a limited number of red clover fields in Oregon. Greenhouse and field studies were conducted to evaluate small broomrape response to common crop and weed species in the Pacific Northwest, and to evaluate a quick-screening method for plant species response to the parasite. In greenhouse studies, plants were grown in a hydroponic polyethylene bag system to allow for continuous visibility of their roots and monitoring of small broomrape seed germination and tubercle development. Results of the greenhouse study were validated in a field contaminated with small broomrape. In the greenhouse polyethylene bag study, small broomrape germinated and tubercles developed on alfalfa, arrowleaf clover, carrot, celery, crimson clover, lettuce, prickly lettuce, red clover, spotted catsear, subterranean clover, white clover, and wild carrot. Small broomrape germinated but did not develop tubercles when grown with barley, birdsfoot trefoil, common vetch, creeping bentgrass, cucumber, field corn, red fescue, flax, Italian ryegrass, nasturtium, oats, orchardgrass, perennial ryegrass, snap bean, sugar pea, sunflower, sweet corn, tall fescue, tomato, and wheat. In the field study, tubercles developed on alfalfa, arrowleaf clover, common vetch, crimson clover, red clover, subterranean clover, and white clover, but small broomrape flower stalks did not emerge in arrowleaf clover or crimson clover. Common vetch was a host for small broomrape in the field study but not in the hydroponic polyethylene bag system, thus, caution is warranted in using the quick screening method to elucidate potential small broomrape host species.

The objective of this study was to examine the effects of crop rotation (spring barley monoculture vs. spring barley–red clover 2-yr rotation), tillage (moldboard plow, chisel plow, no-till), and weed management (intensive, moderate, minimum) on plant–seedbank relationships for 19 weed species. Plant and seedbank density data were collected over 4 yr and analyzed by analysis of variance and correlation analysis to confirm treatment effects on plant–seedbank relationships. The relative frequency (difference between aboveground and seedbank frequency) of many species was more influenced by rotation, whereas species density appeared regulated more by weed management than by other factors. Frequency data confirmed that very few species were ubiquitous over time or treatment, aboveground or in the seedbank. The perennial species, field horsetail, quackgrass, white clover, and perennial sowthistle were more frequent aboveground than in the seedbank. This was also observed for annuals such as common hempnettle, sun spurge, catchweed bedstraw, and annual grasses. Treatment effects on abundance were inconsistent aboveground and in the seedbank across time for 12 of 19 species. The seven species that showed more consistent treatment response for abundance were frequent species present in 50% of the plots both aboveground and in the seedbank. For most species, plant density was correlated with either the previous or current year seedbank, but correlations were rarely of the same magnitude and significance over the years. Common chickweed was the only species for which treatment effects on the plant–seedbank relationship were confirmed for all 4 yr. Correlations between midseason plant populations and subsequent seedbanks confirmed the role of residual populations in replenishing the seedbanks, including those of perennials like quackgrass and dandelion. Overall, plant–seedbank relationships were tenuous for many weed species and varied over time with cropping practices and environment.

Clovers are commonly included as utility plants within mixed grass swards, such as pastures and roadside right-of-ways. As such, they provide supplemental nitrogen, quality forage, and insect habitat. Yet weed control within mixed swards is often hampered by the lack of selective herbicides that are tolerated by clovers. Differential tolerance of legumes to common row-crop and pasture herbicides has previously been reported, yet little information is available that is specific to clover species. Herbicide injury of clover is often inconsistent, hypothetically due to differential species tolerance. Field and greenhouse experiments were conducted with the objective of testing differential tolerance amongst four clover species. Our experiments suggest varying tolerances amongst clover species and common broadleaf herbicides. Only imazaquin control differed due to species; however, treatment by clover interactions were further demonstrated due to variable reductions in clover height. Imazaquin, 2,4-D, 2,4-DB, and triclopyr height reductions differed due to clover species. Differential clover response to herbicide treatment should be an important consideration when managing mixed grass–clover swards and should be accounted for in future research. On a more practical level, our experiments demonstrate a range of herbicides that effectively control clover species, including atrazine, dicamba, clopyralid, 2,4-D, triclopyr, metsulfuron, and trifloxysulfuron. However, results suggest that 2,4-DB, imazethapyr, and bentazon are candidate herbicides for weed control in scenarios in which clover is a desirable crop.

Mesotrione is under evaluation for registration in turfgrass for weed control, but often requires repeat treatments. Previous research in agricultural crops indicates tank mixtures with mesotrione improve weed control. Greenhouse trials were conducted in Blacksburg, VA, with 7 mo-old white clover, and field trials in Knoxville, TN, evaluated control of 5-yr old white clover in a stand of common bermudagrass. Data from both field and greenhouse trials indicate mesotrione applied in combination with bromoxynil, carfentrazone, or simazine control white clover better than any of these herbicides applied alone. Combinations of bromoxynil, carfentrazone, and simazine with mesotrione also decreased relative chlorophyll index and white clover fresh weights in agreement with visually estimated control.

Progress in bioherbicide development has been hindered by the strict moisture and temperature requirements of the living active ingredient. Application of a jute fabric to areas treated with a Sclerotinia minor granular bioherbicide improved broadleaf weed control and broadened the effective application period to include the warm summer season. When turfgrass plots treated with the bioherbicide were covered with burlap fabric for 3 d, broadleaf weed (dandelion, white clover, broadleaf plantain, buckhorn plantain, ground ivy, and prostrate knotweed) control was greatly enhanced. The cover was made of natural jute fibers that retained water but had sufficient transparency to allow 33% light penetration for continued growth of the grass. Virulence of the bioherbicide was maintained under elevated temperatures that would otherwise reduce efficacy. The bioherbicide was ineffective in the summer unless covered, but dandelion density, broadleaf weed ground cover, and dandelion survival were all reduced by the bioherbicide when plots were covered, even if applications were made in July. The efficacy of the bioherbicide was also enhanced under favorable conditions, and covering permitted reduced application rates without loss of efficacy. When applied at a rate of 20 g/m2 and covered, S. minor granules exerted significantly greater biocontrol of dandelion than 40 g/m2 without covering. Covering for up to 5 d did not cause any adverse effects on the turfgrass. This approach may overcome one obstacle to the commercialization of the Sclerotinia minor bioherbicide, permitting its deployment under challenging environmental conditions.