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In vivo measurements of the production rates of bacteria in the rumen

Published online by Cambridge University Press:  27 March 2009

U. B. Singh ,
A. Varma ,
D. N. Verma ,
M. Lal  and
S. K. Ranjhan
U. B. Singh
Affiliation:
Division of Animal Nutrition, Indian Veterinary Research Institute, Izatnagar (U.P.), India
A. Varma
Affiliation:
Division of Animal Nutrition, Indian Veterinary Research Institute, Izatnagar (U.P.), India
D. N. Verma
Affiliation:
Division of Animal Nutrition, Indian Veterinary Research Institute, Izatnagar (U.P.), India
M. Lal
Affiliation:
Division of Animal Nutrition, Indian Veterinary Research Institute, Izatnagar (U.P.), India
S. K. Ranjhan
Affiliation:
Division of Animal Nutrition, Indian Veterinary Research Institute, Izatnagar (U.P.), India
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Summary

The production rates of bacteria have been measured in the rumen of zebu calves and buffalo calves. The animals were fed green oat continuously at 2 h intervals. [35S]sodium sulphate was fed to the animals for 5 days at 2 h intervals by mixing in the feed. On the sixth day the radioactive feed was stopped and replaced by non-radioactive feed. The decline in the specific radioactivity of bacterial cells in the rumen as a function of time was taken for calculating t/2. Simultaneously rumen volume was determined and pool size of bacterial cells was calculated in the rumen. A mathematical equation was applied to calculate the production rates of bacteria. The production rates of bacteria, on average, were 100·5 and 72·3 mg/min for buffalo calves and zebu calves, respectively.

Information

Type
Research Article
Information
The Journal of Agricultural Science , Volume 81 , Issue 2 , October 1973 , pp. 349 - 351
Copyright
Copyright © Cambridge University Press 1973

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References

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