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Detection and identification of Diphyllobothrium nihonkaiense plerocercoids from wild Pacific salmon (Oncorhynchus spp.) in Japan

Published online by Cambridge University Press:  25 March 2010

J. Suzuki ,
R. Murata ,
K. Sadamasu  and
J. Araki
J. Suzuki*
Affiliation:
Division of Clinical Microbiology, Department of Microbiology, Tokyo Metropolitan Institute of Public Health, Tokyo, Japan
R. Murata
Affiliation:
Division of Clinical Microbiology, Department of Microbiology, Tokyo Metropolitan Institute of Public Health, Tokyo, Japan
K. Sadamasu
Affiliation:
Division of Clinical Microbiology, Department of Microbiology, Tokyo Metropolitan Institute of Public Health, Tokyo, Japan
J. Araki
Affiliation:
Meguro Parasitological Museum, Tokyo, Japan
*
*Fax: +81-3-3368-4060 E-mail: Jun_Suzuki@member.metro.tokyo.jp
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Abstract

We investigated the risk of diphyllobothriasis from ingestion of wild Pacific salmon in Japan by surveying Diphyllobothrium plerocercoids in 182 salmon samples obtained from Japan. The plerocercoids were not detected in chum salmon (Oncorhynchus keta) (0/26), called Akizake in Japan, caught between September and November. However, the detection rate of plerocercoids in chum salmon, called Tokishirazu in Japan, caught between early April and June, was 51.1% (24/47) with an average of two plerocercoid larvae per fish. The detection rates of cherry salmon (Oncorhynchus masou) and pink salmon (Oncorhynchus gorbuscha) were 12.2% (10/82) and 18.5% (5/27), respectively, and the average number of plerocercoids per fish was 0.45 (37 larvae/82 fishes) and 0.22 larvae (6 larvae/27 fishes), respectively. Plerocercoids isolated from O. keta and O. masou were identified as Diphyllobothrium nihonkaiense on the basis of molecular analysis of the cox1 and nad3 genes. Moreover, four tapeworms (three from O. keta and one from O. masou) were obtained by infecting golden hamsters with plerocercoids. The morphological features of these tapeworms were similar to those of D. nihonkaiense isolated from humans. Therefore, we think that O. keta and not O. masou is the most important source of plerocercoid infections in Japan.

Information

Type
Research Papers
Information
Journal of Helminthology , Volume 84 , Issue 4 , December 2010 , pp. 434 - 440
Copyright
Copyright © Cambridge University Press 2010

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