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Neuropeptide F (Moniezia expansa): localization and characterization using specific antisera

Published online by Cambridge University Press:  06 April 2009

A. G. Maule ,
C. Shaw ,
D. W. Halton ,
G. P. Brennan ,
C. F. Johnston  and
S. Moore
A. G. Maule
Affiliation:
Comparative Neuroendocrinology Research Group, Schools of 1 Biology and Biochemistry
C. Shaw
Affiliation:
Clinical Medicine, The Queen's University of Belfast, Belfast BT7 INN, U.K.
D. W. Halton
Affiliation:
Comparative Neuroendocrinology Research Group, Schools of 1 Biology and Biochemistry
G. P. Brennan
Affiliation:
Comparative Neuroendocrinology Research Group, Schools of 1 Biology and Biochemistry
C. F. Johnston
Affiliation:
Clinical Medicine, The Queen's University of Belfast, Belfast BT7 INN, U.K.
S. Moore
Affiliation:
Peninsula Laboratories Europe Ltd, Merseyside WA9 3AJ, U.K.
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Summary

Immunocytochemical techniques used in conjunction with confocal scanning laser microscopy (CSLM) and electron microscopy have been used to demonstrate, for the first time, the distribution of the parasitic platyhelminth neuropeptide, neuropeptide F (NPF) in the cestode, Moniezia expansa. Antisera were raised to intact NPF(1–39) and to the C-terminal decapeptide of NPF(30–39). These antisera were characterized and validated for use in both immunocytochemistry and radioimmunoassay (RIA). NPF immunoreactivity (IR) was detected using both antisera throughout all of the major components of the central and peripheral nervous systems of the worm. The pattern of NPF-IR was found to mirror the IR obtained using a C-terminally directed pancreatic polypeptide (PP) antiserum and FMRFamide antisera; blocking studies using these antisera revealed that FMRFamide and PP antisera cross-react with NPF(M. expansa). RIA of acid-alcohol extracts of the worm measured 114 ng/g using the C-terminal NPF antiserum and 56 ng/g using the whole-molecule-directed antiserum. While the C-terminally-directed NPF antiserum cross-reacts with NPF-related peptides from other invertebrates, the whole-molecule-directed NPF antiserum is specific for NPF(M. expansa). The C-terminal NPF antiserum has potential for use in the identification and purification of NPF analogues from other platyhelminth parasites.

Keywords

Moniezia expansaimmunocytochemistryimmunogold labellingradioimmunoassayneuropeptide Fneuropeptide F antiserapancreatic polypeptideFMRFamide

Information

Type
Research Article
Information
Parasitology , Volume 105 , Issue 3 , December 1992 , pp. 505 - 512
Copyright
Copyright © Cambridge University Press 1992

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