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Two-dimensional crystallization of membrane proteins

Published online by Cambridge University Press:  17 March 2009

W. Kühlbrandt
W. Kühlbrandt
Affiliation:
EMBL, Meyerhofstr. 1, D-6900 Heidelberg, Germany
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Extract

In spite of several great breakthroughs, the overall rate of progress in determining high-resolution structures of membrane proteins has been slow. This is entirely due to the scarcity of suitable, well-ordered crystals. Most membrane proteins are multimeric complexes with a composite molecular mass in excess of 50000 Da which puts them outside the range of current solution NMR techniques. For the foreseeable future, detailed information about the structure of large membrane proteins will therefore depend on crystallographic methods.

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Research Article
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Quarterly Reviews of Biophysics , Volume 25 , Issue 1 , February 1992 , pp. 1 - 49
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Copyright © Cambridge University Press 1992

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