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Possible regulation of Oct60 transcription by a positive feedback loop in Xenopus oocytes

Published online by Cambridge University Press:  28 November 2012

Keisuke Morichika ,
Makoto Sugimoto ,
Katsuki Yasuda  and
Tsutomu Kinoshita
Keisuke Morichika*
Affiliation:
Department of Life Science, School of Science, Rikkyo University, 3–34–1 Nishi-ikebukuro, Toshima, Tokyo 178–8501, Japan.
Makoto Sugimoto
Affiliation:
Department of Life Science, School of Science, Rikkyo University, 3–34–1 Nishi-ikebukuro, Toshima, Tokyo 178–8501, Japan.
Katsuki Yasuda
Affiliation:
Department of Bioscience, School of Science and Technology, Kwansei Gakuin University, 2–1 Gakuen, Sanda, Hyogo, 669–1337, Japan.
Tsutomu Kinoshita
Affiliation:
Department of Life Science, School of Science, Rikkyo University, 3–34–1 Nishi-ikebukuro, Toshima, Tokyo 178–8501, Japan.
*
All correspondence to: Keisuke Morichika. Department of Life Science, School of Science, Rikkyo University, 3–34–1 Nishi-ikebukuro, Toshima, Tokyo 178–8501, Japan. Tel: +81 3 3985 4766. Fax: +81 3 3985 4766. e-mail: keisuke.morichika@rikkyo.ac.jp
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Summary

The POU family subclass V (POU-V) proteins have important roles in maintaining cells in an undifferentiated state. In Xenopus, expression of the POU-V protein Oct60 was detected in oocytes and was found to decrease in blastula- to gastrula-stage embryos. In addition, Oct60 overexpression inhibits some signals in early embryogenesis, including Activin/Nodal, BMP, and Wnt signalling. In this report, we analysed mechanisms of Oct60 promoter activation and discovered that Oct60 transcription was activated ectopically in somatic nuclei by oocyte extract treatment. Promoter assays demonstrated that Oct60 transcription was activated in oocytes specifically and that this activation was dependent on an Octamer-Sox binding motif. ChIP assays showed that the Oct60 protein binds the motif. These results suggest that Oct60 transcription is regulated by a positive-feedback loop in Xenopus oocytes.

Keywords

Oct60OogenesisPromoter analysisPOU family class VSelf-regulation
Type
Research Article
Information
Zygote , Volume 22 , Issue 2 , May 2014 , pp. 266 - 274
Copyright
Copyright © Cambridge University Press 2012 

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