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Chapter 1 - Flow Cytometry in Clinical Haematopathology

Basic Principles and Data Analysis of Multiparameter Data Sets

Published online by Cambridge University Press:  30 January 2025

By
Francis Lacombe  and
Marie-Christine Béné
Edited by
Anna Porwit  and
Marie Christine Béné
Anna Porwit
Affiliation:
Lunds Universitet, Sweden
Marie Christine Béné
Affiliation:
Université de Nantes, France
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Summary

This chapter is an introduction to flow cytometry aimed at newcomers in the field but also intended as a refresher for seasoned flow cytometrists confronted with unexpected data related to physical interferences, compensation problems, autofluorescence or aiming at harmonising instruments. It also provides counsel on panel building, sample handling and data display, fundamental points to consider in setting up new protocols.

Keywords

flow cytometrymultiparameterfluorochromesinstrumentssettingspanelscompensationgatinghistogramsdot plots
Type
Chapter
Information
Multiparameter Flow Cytometry in the Diagnosis of Hematologic Malignancies , pp. 1 - 14
Publisher: Cambridge University Press
Print publication year: 2025

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