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8-pCPT, an Epac activator, impairs conditioned place preference based on nucleus accumbens amphetamine in rats

Published online by Cambridge University Press:  01 August 2013

Sung Woo Park ,
Ali Roohbakhsh  and
Richard J. Beninger
Sung Woo Park
Affiliation:
Centre for Neuroscience Studies, Queen's University, Kingston, ON, Canada
Ali Roohbakhsh
Affiliation:
Pharmaceutical Research Center, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
Richard J. Beninger*
Affiliation:
Centre for Neuroscience Studies, Queen's University, Kingston, ON, Canada Department of Psychology, Queen's University, Kingston, ON, Canada Department of Psychiatry, Queen's University, Kingston, ON, Canada
*
Dr. Richard J. Beninger, Department of Psychology, 62 Arch St., Queen's University, Kingston, ON, Canada K7L 3N6. Tel: +1 613 533 2486; Fax: +1 613 533 2499; E-mail: beninger@queensu.ca
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Abstract

Objectives

Dopamine receptor-mediated 3′,5′-cyclic adenosine monophosphate (cAMP)-dependent intracellular signalling is important for reward-related learning. cAMP activates cAMP-dependent protein kinase (PKA) and exchange protein directly activated by cAMP (Epac). We tested the hypothesis that reward-related learning may be mediated by Epac.

Methods

We evaluated conditioned place preference (CPP) on the basis of nucleus accumbens (NAc) injections of amphetamine (20 μg/0.5 μl/side) plus Sp-adenosine 3′,5′-cyclic monophosphorothioate triethylamanine (Sp-cAMPS) (0.1, 1.0, 10, 15, 20 μg/0.5 μl/side), an activator of both PKA and Epac, or amphetamine (20 μg) plus 8-(4-chlorophenylthio)-2′-O-methyladenosine-3′,5′-cyclic monophosphate (8-pCPT) (0.73, 1.27, 1.45, 2.89, 5.78, 11.56 μg/0.5 μl/side), an activator of Epac.

Results

In agreement with previous results, Sp-cAMPS dose-dependently impaired CPP. 8-pCPT impaired CPP at one dose (1.45 μg/0.5 μl/side) and we replicated this effect three times.

Conclusion

The results implicate Epac in the acquisition of reward-related learning.

Keywords

amphetamineexchange protein directly activated by cAMPincentive learningsecond messengers
Type
Original Articles
Information
Acta Neuropsychiatrica , Volume 26 , Issue 2 , April 2014 , pp. 104 - 111
Copyright
Copyright © Scandinavian College of Neuropsychopharmacology 2013 

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