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Chronic lipopolysaccharide infusion fails to induce depressive-like behaviour in adult male rats

Published online by Cambridge University Press:  20 February 2015

Christina Weide Fischer ,
Nico Liebenberg Open the ORCID record for Nico Liebenberg [Opens in a new window] ,
Anne Mette Madsen ,
Heidi Kaastrup Müller ,
Sten Lund  and
Gregers Wegener
Christina Weide Fischer*
Affiliation:
Translational Neuropsychiatry Unit, Aarhus University, Risskov, Denmark
Nico Liebenberg
Affiliation:
Translational Neuropsychiatry Unit, Aarhus University, Risskov, Denmark
Anne Mette Madsen
Affiliation:
The National Research Centre for the Working Environment, Copenhagen, Denmark
Heidi Kaastrup Müller
Affiliation:
Translational Neuropsychiatry Unit, Aarhus University, Risskov, Denmark
Sten Lund
Affiliation:
Medical Research Laboratory, Medical Department M (Endocrinology and Diabetes), Aarhus University Hospital, Denmark
Gregers Wegener
Affiliation:
Translational Neuropsychiatry Unit, Aarhus University, Risskov, Denmark
*
Christina Weide Fischer, Translational Neuropsychiatry Unit, Aarhus University, Skovagervej 2, 8240 Risskov, Denmark. Tel: +457 847 1122; Fax: +45 8471124; E-mail: Christina.weide.fischer@clin.au.dk
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Abstract

Background

Chronic inflammation is implicated in numerous diseases, including major depression and type 2 diabetes mellitus (T2DM). Since depression and T2DM often co-exist, inflammatory pathways are suggested as a possible link. Hence, the establishment of an immune-mediated animal model would shed light on mechanisms possibly linking depression and metabolic alterations.

Objective

In this study we investigated a behavioural and metabolic paradigm following chronic infusion with low doses of lipopolysaccharide (LPS) using osmotic minipumps in male rats.

Methods

Behavioural testing consisted of evaluating activity level in the open field and depressive-like behaviour in the forced swim test. Metabolic assessment included measurement of body weight, food and water intake, and glucose and insulin levels during an oral glucose tolerance test.

Results

LPS-infused rats showed acute signs of sickness behaviour, but chronic LPS infusion did not induce behavioural or metabolic changes.

Conclusion

These results suggest that although inflammation is immediately induced as indicated by acute sickness, 4 weeks of chronic LPS administration via osmotic minipumps did not result in behavioural changes. Therefore, this paradigm may not be a suitable model for studying the underlying mechanisms that link depression and T2DM.

Keywords

central nervous systemdepressionneuroendocrinologypsychoneuroimmunology
Type
Short Communication
Information
Acta Neuropsychiatrica , Volume 27 , Issue 3 , June 2015 , pp. 189 - 194
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Copyright
© Scandinavian College of Neuropsychopharmacology 2015 

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