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Serum lipid peroxidation markers are correlated with those in brain samples in different stress models

Published online by Cambridge University Press:  11 July 2013

Asuman Celikbilek ,
Ayse Yesim Gocmen ,
Nermin Tanik ,
Nazmi Yaras ,
Piraye Yargicoglu  and
Saadet Gumuslu
Asuman Celikbilek
Affiliation:
Department of Neurology
Ayse Yesim Gocmen
Affiliation:
Department of Biochemistry, Medical School, Bozok University, Yozgat, Turkey
Nermin Tanik
Affiliation:
Department of Neurology
Nazmi Yaras
Affiliation:
Department of Biophysics
Piraye Yargicoglu
Affiliation:
Department of Biophysics
Saadet Gumuslu*
Affiliation:
Department of Biochemistry, Medical School, Akdeniz University, Antalya, Turkey
*
Saadet Gumuslu, Department of Biochemistry, Faculty of Medicine, Akdeniz University, 07070 Antalya, Turkey. Tel: +00 (90) 242 2496896; Fax: +00 (90) 242 2274495; E-mail: saadet.gumuslu@hotmail.com
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Abstract

Objective

Stress can stimulate increased production of oxygen radicals. We investigated the correlations between serum levels of lipid peroxidation markers and those in brain samples in different stress models.

Methods

Animals (n = 96) were divided equally into eight groups: a control group and groups treated with vitamin E (Vit E); exposed to immobilisation stress; exposed to immobilisation stress and treated with Vit E; exposed to cold stress; exposed to cold stress and treated with Vit E; exposed to both immobilisation and cold stress; and a final group exposed to both immobilisation and cold stress and treated with Vit E. Thiobarbituric acid-reactive substance (TBARS) in brain samples and levels of TBARS, corticosterone, conjugated dienes (CD), lipids, and paraoxonase-1 (PON1) activity in serum were analysed.

Results

Serum corticosterone (p < 0.001), CD (p < 0.05), lipid (p < 0.05) levels, and brain TBARS (p < 0.05) levels were significantly higher in all stress groups than in controls, and the elevated levels were reversed in the Vit E-treated stress groups (p < 0.05). Serum PON1 activity was not different among the groups (p > 0.05). Serum TBARS levels increased significantly in all stress groups (p < 0.05), but this elevation was only reversed in the group exposed to both immobilisation and cold stress and treated with Vit E (p < 0.001).

Conclusion

These results suggest that serum levels of lipid peroxidation markers can be determined readily and may be useful as indicators to evaluate the effects of oxidative stress in the brain.

Keywords

lipid peroxidationoxidative stressoxygen radicalsvitamin E
Type
Original Articles
Information
Acta Neuropsychiatrica , Volume 26 , Issue 1 , February 2014 , pp. 51 - 57
Copyright
Copyright © Scandinavian College of Neuropsychopharmacology 2013 

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