A Non-Dispersive X-ray Fluorescence Unit for the Analysis of Biological Tissue Sections

Abstract

An X-ray fluorescence analysis unit has been designed and built especially for the measurement of certain mineral elements in individual biological tissue sections. Such a section may contain in the neighborhood of 10^-10 grams of an element of interest, in a concentration in the range of 1-100 p.p.m.

The unit consists of a special high-power X-ray tube with a builtin interchangeable secondary radiator, which irradiates the speciman with the characteristic lines of the radiator element) and a proportional counter and pulse-height analyzer system, which provides analysis of the X-ray spectrum emitted by the specimen. Because the emitted spectrum is greatly simplified by the use of an appropriate radiator element, a diffracting crystal can be omitted, permitting a great increase in absolute sensitivity.

The system is feasible only because of two peculiarities of our biological specimens: they are so thin that matrix effects are negligible, and they consist essentially of a few mineral elements in a light matrix.

Design considerations, calibration procedures, procedures for the analysis of the observed proportional counter pulse-height spectra and results to date will be discussed.